Review
Chemistry, Analytical
Hafiz Muhammad Junaid, Shahid Munir, Madeeha Batool
Summary: Ionic liquid mediated carbon dots (IL-CDs) have emerged as new sensing probes with unique physical, chemical and optical properties, as well as ecofriendly nature. This review evaluates the use of IL-CDs as sensing probes for various chemical species, highlighting the structural differences, versatility in sensing modes, types of entities being sensed, and performance efficiency compared to ordinary CDs.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Physical
Vaibhavkumar N. Mehta, Milltal L. Desai, Hirakendu Basu, Rakesh Kumar Singhal, Suresh Kumar Kailasa
Summary: This article discusses the importance of integrating fluorescent nanomaterials with analytical tools, introduces the applications of fluorescent nanomaterials in the analysis of metal ions and cell bioimaging, and emphasizes their advantages such as high selectivity, sensitivity, and low detection limits.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Analytical
Jing Li, Fan Mo, Lei Guo, Junhua Huang, Zheng Lu, Qin Xu, Hongbo Li
Summary: The study proposes a novel design using 3-thiopropionic acid-capped CdTe quantum dots (QDs) for copper ion photoelectrochemical sensing, which offers a wider linear range and lower detection limit, as well as additional advantages in terms of cost-effectiveness, simplicity, and selectivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Yongchao Du, Yaxi Li, Yunliang Liu, Naiyun Liu, Yuanyuan Cheng, Qiuzhong Shi, Xiang Liu, Zhimin Tao, Yumeng Guo, Jianguo Zhang, Najmeh Askaria, Haitao Li
Summary: The research developed a carbon dot sensor using corn stalk powder as a carbon precursor, which can be used for the detection of Fe3+ ions and biological cell imaging. The sensor showed high selectivity and sensitivity towards Fe3+ ions, while also exhibiting low cytotoxicity and good biocompatibility.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yu-Syuan Lin, Zong-Yu Yang, Anisha Anand, Chih-Ching Huang, Huan-Tsung Chang
Summary: This study successfully synthesized hydrophobic carbon dots (TO-CDs) and explored their applications in detecting sodium copper chlorophyllin (SCC) and Cu2+ ions. TO-CDs exhibited good detection performance and selectivity toward SCC and Cu2+ ions. These findings are of great significance for the rapid screening of Cu2+ ions in environmental samples and SCC in foods.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agricultural Engineering
Xinyan Hou, Jikun Xu, Pengfei Zhou, Lin Dai, Jiaming Zhang, Xiao Xiao, Chuntao Zhang, Kaifu Huo
Summary: In this study, a solvent-engineered method was proposed to synthesize multicolor carbon dots with distinct nanostructures and partially surface-functionalized domains. These carbon dots exhibited rapid and accurate fluorometric detection of metal ions including Fe3+, Ag+, Cu2+, and Al3+. The findings highlight the importance of lignin molecular state, quantum size, zeta potential, electrostatic, and cation-π interactions in determining the fluorescence properties and sensitivity to polar solvents. This research not only provides a scalable approach for nanoscale design using lignin, but also contributes to the development of multicolor carbon dots for specific metal-ion recognition.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Analytical
Hari Krishna Sadhanala, Saurav Aryal, Kusha Sharma, Ziv Orpaz, Shulamit Michaeli, Aharon Gedanken
Summary: In this study, photoluminescent N-doped carbon dots were synthesized and used for the selective detection and imaging of intracellular magnesium ions. This is the first report of using carbon dots for sensing and imaging of magnesium ions in cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Jorge Espina-Casado, Tania Fontanil, Alfonso Fernandez-Gonzalez, Santiago Cal, Alvaro J. Obaya, Marta Elena Diaz-Garcia, Rosana Badia-Laino
Summary: Carbon dots were synthesized from glutathione and surface-modified with beta-Cyclodextrin and FP, showing potential applications as drug carriers. The fluorescence changes allowed distinction of cancer cells with no alterations in cell morphology, enabling bioimaging and cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Qinhong Yin, Xiaolan Yang, Lihua Yang, Dezhi Yang, Yaling Yang, Yanqin Zhu
Summary: In this study, iodine and copper-doped carbon dots were synthesized using a simple hydrothermal process, exhibiting peroxidase-like activity and high catalytic effectiveness. The results showed that these nanozymes can be used to develop a colorimetric sensor for the quick and accurate detection of morphine.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Ahmed Mohammed Ali Abdulkhaleq, Yixu Zhao, Kun Qian, Xueshi Wang, Zhongfu Zuo, Xibin Zhou
Summary: Researchers have developed a fluorescent probe based on carbon dots for the sensitive and selective detection and imaging of endogenous and exogenous peroxynitrite (ONOO-) in cells and animal models. The probe shows low cytotoxicity and has been successfully applied for imaging of exogenous and endogenous ONOO- in zebrafish and mice.
Article
Chemistry, Analytical
Xiaorui Dong, Lihong Shi, Wei Zhou, Shaomin Shuang
Summary: Dual-emission carbon dots (D-CDs) were developed for ratiometric recognition of Co2+ and EDTA, displaying continuous color changes and visual quantification. The D-CDs exhibited dual emissions at 562 nm and 610 nm under 450 nm excitation. The fluorescence of D-CDs can be quenched by Co2+ and recovered by EDTA, enabling visual quantification of Co2+ and EDTA using a smartphone app.
MICROCHEMICAL JOURNAL
(2023)
Article
Spectroscopy
Min Tian, Junqiu Zhang, Yaoming Liu, Yingte Wang, Yong Zhang
Summary: A facile, economical and green method for the preparation of nitrogen-doped carbon dots (N-CDs) was established via hydrothermal synthesis in this study. The prepared N-CDs showed high sensitivity towards cobalt (II) ion (Co2+) and demonstrated potential applications in water sample analysis and biological tissue imaging.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Feng-Yi Wu, Ji-Liang Yang, You-Sheng Ye, Ya-Qiong Kong, Rong Wu, Hai-Yan Wang, Xin Wang
Summary: Recently, MoS2 quantum dots (QDs) have gained widespread attention as a potential luminescent material. However, research on the multicolor emission of MoS2 QDs has been limited. In this study, an in situ iodine doping strategy is used to synthesize tunable-photoluminescent (PL) MoS2 QDs. The emission wavelength of the MoS2 QDs can be tuned by fine iodine doping, and the as-synthesized I-MoS2 QDs combined with Fe3+ serve as a fluorescence sensor for F- ions in water. The fluorescence probe exhibits a linear response to F- ions and has a low limit of detection.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Pooja Chauhan, Deepa Mundekkad, Amitava Mukherjee, Savita Chaudhary, Ahmad Umar, Sotirios Baskoutas
Summary: In this study, carbon dots synthesized under different temperature conditions were found to exhibit high selectivity and sensitivity towards tyrosine in amino acid sensing. The binding efficiency and biocompatibility of the developed carbon dots with other biologically active components were evaluated.
Article
Chemistry, Analytical
Zanling Huang, Shuqi Zhu, Abebe Reda Woldu, Wenhua Gao, Jing-Xin Jian, Paul K. Chu, Qing-Xiao Tong, Liangsheng Hu
Summary: This study demonstrates a bimetallic ion co-catalyzed electrochemical glucose detection by introducing Co(II) and Cu(II) into an alkaline solution. By using nitrogen-doped carbon nanotubes loaded on carbon cloth as the working electrode, the system achieves high sensitivity, low detection limit, good selectivity, and stability. Through systematic electrochemical and structural analysis, the mechanism of Co(II) and Cu(II) co-catalysis in glucose detection is elucidated.
MICROCHEMICAL JOURNAL
(2023)
Article
Surgery
Yuyang Yang, Qiong Li, Ting Yu, Jialiang Mao, Yanling Wang, Wenjie Wu
Summary: We evaluated the efficacy and clinical outcomes of bicanalicular-nasal silicone stents for deep canalicular lacerations and found that they have significant advantages in anatomical restoration. The results of anatomical, functional, and cosmetic evaluations showed that bicanalicular-nasal silicone stent placement effectively restored the anatomy of the lacrimal system, improved eyelid function, and achieved satisfactory cosmetic realignment.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Letter
Surgery
Wenjie Wu, Haiwen Lu, Guanghao Piao, Rui Liu
ASIAN JOURNAL OF SURGERY
(2023)
Article
Genetics & Heredity
Pu-Gen An, Wen-Jie Wu, Yu-Fang Tang, Jie Zhang
Summary: Adenoid cystic carcinoma (ACC) is a common malignancy of the major salivary glands with high recurrence rate and poor prognosis. Single-cell RNA sequencing was performed to analyze tumor heterogeneity, immune cell infiltration, and cell communication in ACC tissues. The heterogeneity of epithelial cells in ACC tissues was significantly higher than in normal tissues, and immune cells were almost absent. We identified malignant cell clusters, abnormal immune cell composition, and enhanced communication between cells in the tumor tissue.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Ophthalmology
Xiaobao Liu, Mengting Yu, Yue Huang, Qiong Li, Wenjie Wu
Summary: This study evaluated the effects of primary posterior continuous curvilinear capsulorhexis (PPCCC) on intraocular lens (IOL) tilt and decentration after cataract surgery. The results showed that the PPCCC group had less IOL decentration at 3 months postoperatively, suggesting better IOL centrality with PPCCC.
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2023)
Article
Physics, Multidisciplinary
F. P. An, W. D. Bai, A. B. Balantekin, M. Bishai, S. Blyth, G. F. Cao, J. Cao, J. F. Chang, Y. Chang, H. S. Chen, H. Y. Chen, S. M. Chen, Y. Chen, Y. X. Chen, Z. Y. Chen, J. Cheng, Z. K. Cheng, J. J. Cherwinka, M. C. Chu, J. P. Cummings, O. Dalager, F. S. Deng, Y. Y. Ding, X. Y. Ding, M. V. Diwan, T. Dohnal, D. Dolzhikov, J. Dove, H. Y. Duyang, D. A. Dwyer, J. P. Gallo, M. Gonchar, G. H. Gong, H. Gong, W. Q. Gu, J. Y. Guo, L. Guo, X. H. Guo, Y. H. Guo, Z. Guo, R. W. Hackenburg, Y. Han, S. Hans, M. He, K. M. Heeger, Y. K. Heng, Y. K. Hor, Y. B. Hsiung, B. Z. Hu, J. R. Hu, T. Hu, Z. J. Hu, H. X. Huang, J. H. Huang, X. T. Huang, Y. B. Huang, P. Huber, D. E. Jaffe, K. L. Jen, X. L. Ji, X. P. Ji, R. A. Johnson, D. Jones, L. Kang, S. H. Kettell, S. Kohn, M. Kramer, T. J. Langford, J. Lee, J. H. C. Lee, R. T. Lei, R. Leitner, J. K. C. Leung, F. Li, H. L. Li, J. J. Li, Q. J. Li, R. H. Li, S. Li, S. C. Li, W. D. Li, X. N. Li, X. Q. Li, Y. F. Li, Z. B. Li, H. Liang, C. J. Lin, G. L. Lin, S. Lin, J. J. Ling, J. M. Link, L. Littenberg, B. R. Littlejohn, J. C. Liu, J. L. Liu, J. X. Liu, C. Lu, H. Q. Lu, K. B. Luk, B. Z. Ma, X. B. Ma, X. Y. Ma, Y. Q. Ma, R. C. Mandujano, C. Marshall, K. T. McDonald, R. D. McKeown, Y. Meng, J. Napolitano, D. Naumov, E. Naumova, T. M. T. Nguyen, J. P. Ochoa-Ricoux, A. Olshevskiy, H. -R. Pan, J. Park, S. Patton, J. C. Peng, C. S. J. Pun, F. Z. Qi, M. Qi, X. Qian, N. Raper, J. Ren, C. Morales Reveco, R. Rosero, B. Roskovec, X. C. Ruan, B. Russell, H. Steiner, J. L. Sun, T. Tmej, K. Treskov, W. -H. Tse, C. E. Tull, B. Viren, V. Vorobel, C. H. Wang, J. Wang, M. Wang, N. Y. Wang, R. G. Wang, W. Wang, X. Wang, Y. Wang, Y. F. Wang, Z. Wang, Z. Wang, Z. M. Wang, H. Y. Wei, L. H. Wei, W. Wei, L. J. Wen, K. Whisnant, C. G. White, H. L. H. Wong, E. Worcester, D. R. Wu, Q. Wu, W. J. Wu, D. M. Xia, Z. Q. Xie, Z. Z. Xing, H. K. Xu, J. L. Xu, T. Xu, T. Xue, C. G. Yang, L. Yang, Y. Z. Yang, H. F. Yao, M. Ye, M. Yeh, B. L. Young, H. Z. Yu, Z. Y. Yu, B. B. Yue, V. Zavadskyi, S. Zeng, Y. Zeng, L. Zhan, C. Zhang, F. Y. Zhang, H. H. Zhang, J. L. Zhang, J. W. Zhang, Q. M. Zhang, S. Q. Zhang, X. T. Zhang, Y. M. Zhang, Y. X. Zhang, Y. Y. Zhang, Z. J. Zhang, Z. P. Zhang, Z. Y. Zhang, J. Zhao, R. Z. Zhao, L. Zhou, H. L. Zhuang, J. H. Zou
Summary: We present a new determination of the smallest neutrino mixing angle 013 and the mass-squared difference Delta m232 using a final sample of 5.55 x 106 inverse beta-decay (IBD) candidates with the final-state neutron captured on gadolinium. Compared to the previous Daya Bay results, selection of IBD candidates has been optimized, energy calibration refined, and treatment of back-grounds further improved. The resulting oscillation parameters are sin22013 = 0.0851 +/- 0.0024, Delta m232 = (2.466 +/- 0.060) x 10-3 eV2 for the normal mass ordering or Delta m232 = -(2.571 +/- 0.060) x 10-3 eV2 for the inverted mass ordering.
PHYSICAL REVIEW LETTERS
(2023)
Article
Clinical Neurology
Ngan Yin Chan, Wen Jie Wu, Joey Wing Yan Chan, Kate Ching Ching Chan, Albert Martin Li, Sandra Sau Man Chan, Kit Tai Hau, Yun Kwok Wing
Summary: This study explored the association between sleep and academic performance in a large and representative sample of school children and adolescents in Hong Kong. It found that there is an inverted U-shaped relationship between sleep duration and academic performance, with an optimal level of sleep associated with better academic performance. The findings highlight the importance of systematic sleep education and intervention to encourage optimal sleep patterns for students, which can have a positive effect on their academic performance.
Article
Cell Biology
Wenjie Wu, Jialuo Xu, Dan Gao, Zhenliang Xie, Wenjing Chen, Wenjing Li, Qiang Yuan, Lina Duan, Yuhan Zhang, Xiaoxiao Yang, Yingying Chen, Ziming Dong, Kangdong Liu, Yanan Jiang
Summary: TOPK and YB1 are highly expressed in esophageal cancer and correlate with poor prognosis. TOPK knockout inhibits cell proliferation, and this effect can be reversed by rescuing YB1 expression. TOPK phosphorylates YB1 to activate eEF1A1 transcription, subsequently activating the AKT/mTOR signaling pathway.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Hui Wang, Zichao Wen, Wenjie Wu, Zhexian Sun, Zulfia Kisrieva-Ware, Yiqi Lin, Sicheng Wang, Hansong Gao, Haonan Xu, Peinan Zhao, Qing Wang, George A. Macones, Alan L. Schwartz, Phillip Cuculich, Alison G. Cahill, Yong Wang
Summary: Electromyometrial imaging (EMMI) has been developed to noninvasively and accurately image the 3D uterine electrical activation in sheep. In this study, the authors developed and applied a human EMMI system to image and evaluate 3D uterine electrical activation patterns during human term labor. The system successfully generated noninvasive uterine surface electrical potential maps, electrograms, and activation sequences using up to 192 electrodes, providing detailed 3D images and quantification of uterine contractions.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Electrical & Electronic
Wenjie Wu, Lin Qiu, Xing Liu, Jien Ma, Jian Zhang, Min Chen, Youtong Fang
Summary: This article proposes a model-free sequential predictive control method for an active front-end modular multilevel converter (AFE-MMC). A generalized ultra-local model of the MMC is first established, and a predictor-based neural network (PNN) is deployed to estimate the unmodeled dynamics and perturbations. A sequential predictive control with variable candidate set is proposed to eliminate the weighting factors in the control process. The proposed method does not require knowledge of mathematical models or the identification of weighting factors, resulting in enhanced performance and less tuning work.
IEEE JOURNAL OF EMERGING AND SELECTED TOPICS IN POWER ELECTRONICS
(2023)
Article
Engineering, Electrical & Electronic
Lan Cheng, Lin Qiu, Wenjie Wu, Xing Liu, Jien Ma, Youtong Fang
Summary: This article proposes a virtual voltage vector-based sequential model-free predictive control method for parallel converters, which improves the reliability and robustness of the system by addressing the issue of unbalanced connections between parallel inverters.
IEEE JOURNAL OF EMERGING AND SELECTED TOPICS IN POWER ELECTRONICS
(2023)
Article
Engineering, Electrical & Electronic
Xing Liu, Lin Qiu, Wenjie Wu, Jien Ma, Youtong Fang, Zhouhua Peng, Dan Wang
Summary: This article investigates a novel finite control set model predictive control (FCS-MPC) for sensorless three-phase four-arm modular multilevel converter (MMC). The main objectives of the proposed solution are to avoid the cumbersome online tuning of weighting factors and to achieve the balance of multiple control objectives, allowing a lower control complexity and associated costs.
IEEE JOURNAL OF EMERGING AND SELECTED TOPICS IN POWER ELECTRONICS
(2023)
Article
Genetics & Heredity
Mei-Rong Bai, Hao-Yue Pei, Ying Zhou, Huan-Lei Song, Wei-Hua Pan, Yi-Ming Gong, Wen-Jie Wu, Wen-Wen Yu, Meng-Meng Cui, Bei-Lin Gu, Xun Chu, Wei Cai
Summary: This study aimed to investigate the association between common variants in the JAG1 gene and the risk of biliary atresia (BA) in the Chinese Han population. The study found significant associations between two single nucleotide polymorphisms (rs6077861 and rs3748478) within the JAG1 gene and BA susceptibility. Knockdown of JAG1 homologs in a zebrafish model led to defective biliary development. These findings suggest that JAG1 may be involved in the etiology of BA.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Qing Liu, Qiaoyun Long, Jiayu Zhao, Wenjie Wu, Zexin Lin, Wei Sun, Ping Gu, Tuo Deng, Kerry Martin Loomes, Donghai Wu, Alice P. S. Kong, Jingying Zhou, Alfred S. Cheng, Hannah Xiaoyan Hui
Summary: Through transcriptomic profiling at the single-cell and single-nucleus resolution, we reconstructed the cellular atlas in mouse subcutaneous white adipose tissue and revealed the transcriptomes and intercellular cross-talks during brown remodeling. Our findings identified subpopulations in mature adipocytes, adipose stem and progenitor cells, and endothelial cells, and uncovered their interconversion and reprogramming in response to cold. Our data unveil fundamental changes during cold-evoked adipose browning.
Meeting Abstract
Obstetrics & Gynecology
Zhexian Sun, Hansong Gao, Wenjie Wu, Sicheng Wang, Peinan Zhao, D. Michael Nelson, Pamela K. Woodard, Yong Wang
REPRODUCTIVE SCIENCES
(2023)
Article
Ophthalmology
Xinna Wu, Xiaobao Liu, Suzhen Xiao, Yajing Cai, Mengting Yu, Binqiang Xu, Yanling Wang, Wenjie Wu
Summary: A retrospective case series study demonstrated that primary customized phacoemulsification combined with goniosynechialysis (phaco-GSL) is a safe and effective surgical approach for patients with refractory acute primary angle closure (APAC) and high intraocular pressure (IOP). The study showed significant improvement in visual acuity and reduction in IOP, with minimal complications.
INTERNATIONAL OPHTHALMOLOGY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).