Article
Biochemical Research Methods
Stefano Fornasaro, Alessandro Esposito, Fiorella Florian, Alberto Pallavicini, Luigina De Leo, Tarcisio Not, Cristina Lagatolla, Marica Mezzarobba, Alessia Di Silvestre, Valter Sergo, Alois Bonifacio
Summary: Significant differences were found in the faecal samples of celiac patients and those on a gluten-free diet using SERS spectroscopy and 16S rRNA sequencing analysis. Although there were no significant differences in bacterial composition, the relative intensity of xanthine and hypoxanthine in the SERS spectra of patients on a gluten-free diet was significantly increased.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Mihaela Chis, Alois Bonifacio, Valter Sergo, Calin Cainap, Vasile Chis, Monica Baia
Summary: This study conducted experimental and theoretical analysis of CAP to characterize its structure and vibrational properties, revealing the adsorption geometry and orientation of the molecules on the gold surface. The results showed that a good signal can be obtained even at low concentrations.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Engineering, Electrical & Electronic
Doaa Sulaiman, Alwan M. Alwan, Walid K. Hamoudi
Summary: Bimetallic alloys nanoparticles were synthesized and combined with Si nano-pillars substrates to prepare SERS sensors, showing excellent recognition of ultra-low concentrations of chlorpyrifos solutions, with the highest enhancement factor obtained in Au-Pd sensors.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2021)
Article
Food Science & Technology
Francesco Corrias, Alessandro Atzei, Riccardo Taddeo, Nicola Arru, Mattia Casula, Rachid Salghi, Mariateresa Russo, Alberto Angioni
Summary: This study investigated the bio-distribution and persistence of fipronil and its metabolite in poultry under simulated natural farming conditions. The results showed that inappropriate use of unauthorized pesticides can lead to severe contamination of entire poultry farms, highlighting the importance of proper pesticide management in agricultural practices.
Article
Chemistry, Analytical
Andra-Sorina Tatar, Sanda Boca, Alexandra Falamas, Denisa Cuibus, Cosmin Farcau
Summary: In this study, high-density gold nanostar films were fabricated through self-assembly on polystyrene plates, and their use as solid plasmonic substrates for surface-enhanced optical spectroscopies was explored. The films demonstrated dual-mode surface-enhanced fluorescence and surface-enhanced Raman scattering effects under visible and NIR laser excitation. The experimental results were supported by theoretical modelling, and the potential application of these substrates in the detection of biologically relevant molecules was demonstrated.
Article
Food Science & Technology
Min-Hui Lin, Lin Sun, Fanbin Kong, Mengshi Lin
Summary: This study established a novel, facile, and rapid testing technique using surface-enhanced Raman spectroscopy (SERS) coupled with gold nanostars as a SERS substrate to detect pesticide residues in green tea. The spiky tips of gold nanostars acted as SERS hot spots to enhance Raman signals, while the rough topography increased surface area for better substrate-analyte interactions. The SERS method showed great potential for qualification and quantification of trace contaminants in foods with a detection limit of 0.2 mg/kg for paraquat in green tea samples.
Article
Food Science & Technology
Luyao Wang, Pei Ma, Hui Chen, Min Chang, Ping Lu, Ning Chen, Yanbing Yuan, Nan Chen, Xuedian Zhang
Summary: This study developed a fast, sensitive, economical, and lossless surface-enhanced Raman spectroscopy (SERS)-based method for the detection of residues of widely used insecticides and herbicides, CPF and 2,4-D. The method can accurately detect mixed pesticide residues on an apple surface at a minimum concentration of 0.001 mg L-1, which is below the pesticide residue standard. It has potential for accurate detection of pesticide residues in other agricultural and food industries.
Review
Chemistry, Analytical
Angela I. Lopez-LOrente
Summary: Surface enhanced Raman spectroscopy (SERS) is a powerful technique attracting attention, where the use of different gold nanostructures can enhance the SERS response. The shape of nanostructures and nanogaps play a crucial role in SERS efficiency. The creation of flexible supports for SERS substrates is a recent trend in the field.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Physical
Wuwen Yan, Siyu Chen, Pan Li, Ronglu Dong, Hyun-Hang Shin, Liangbao Yang
Summary: In this study, a platform for monitoring label-free molecules at the single-molecule level using surface-enhanced Raman spectroscopy (SERS) was developed based on the photothermal effect of gold nanorods (GNRs). The results showed that single crystal violet (CV) molecules can be observed blinking for up to 4 minutes using dynamic surface-enhanced Raman spectroscopy (D-SERS). The research provides a novel idea for exploring the temporal and spatial dynamic processes of different reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Physical
Wuwen Yan, Siyu Chen, Pan Li, Ronglu Dong, Hyun-Hang Shin, Liangbao Yang
Summary: In this study, a platform for monitoring single molecules using surface-enhanced Raman spectroscopy (SERS) was developed based on the photothermal effect of gold nanorods (GNRs). By utilizing laser re-irradiation to create nanometer gaps between GNRs, the blinking behavior of single crystal violet (CV) molecules was observed for up to 4 minutes using dynamic surface-enhanced Raman spectroscopy (D-SERS). The confinement of CV molecules in sub-nanometer spaces and the dynamic capture of their orientations provide a new approach for exploring the temporal and spatial dynamic processes of different reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Applied
Lin Sun, Zhilong Yu, Fouad K. Alsammarraie, Min-Hui Lin, Fanbin Kong, Meizhen Huang, Mengshi Lin
Summary: A novel SERS method coupled with CNF-based wipers was developed for rapid detection of pesticide residues in vegetables. The method showed satisfactory performance in detecting ferbam within a few minutes with a detection limit of 50 μg/kg. The enhancement factor of the SERS substrate was calculated to be around 10^4 with satisfactory reproducibility.
Article
Biochemistry & Molecular Biology
Fahad M. M. Aldosari
Summary: This study investigated the size dependency of gold nanoparticles (AuNPs) on their surface-enhanced Raman scattering (SERS) activity by using labeled AuNPs. The results showed that the SERS signal increases with the increasing size of AuNPs, and the preferred candidates for Raman labelling of spherical gold within the range of 5 nm to 100 nm are BPT and 4-AATP.
Article
Spectroscopy
Yazhou Qin, Shusheng Yin, Mingjie Chen, Weixuan Yao, Yingsheng He
Summary: In this study, a highly sensitive surface-enhanced Raman scattering (SERS) method was developed for detecting the opioid drug fentanyl and its analogs. By controlling the morphology of the prepared nanocrystals and performing density functional theory (DFT) calculations, the differentiation of different analogs was achieved.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Cristina Gellini, Marina Macchiagodena, Marco Pagliai
Summary: The adsorption geometry of alizarin on silver nanoparticles was studied using density functional theory calculations, considering different charged species present in solution at different pH values. The results accurately reproduced the measured SERS spectra and elucidated the adsorption geometry of the dye on the silver substrate.
Review
Chemistry, Multidisciplinary
Changchun Wen, Liping Wang, Li Liu, Xing-Can Shen, Hua Chen
Summary: This review discusses the recent progress in the application of gold-based nanomaterials as SERS probes in biomedical research, primarily for in vivo disease diagnosis and imaging. Gold nanomaterials have outstanding surface plasmon resonance effects, structural adjustability, and good biocompatibility.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Analytical
Muhammad Muhammad, Chang-sheng Shao, Qing Huang
Summary: This study successfully developed a label-free biosensor for detecting interleukin-6 and achieved quantitative evaluation in a low concentration range. The research indicates that the aptamer-based SERS biosensor can be a promising tool for quick and convenient medical diagnosis applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Food Science & Technology
Mohammed Sharif Swallah, Xiaoqing Yang, Jiaxin Li, Joseph Kudadam Korese, Sainan Wang, Hongliang Fan, Hansong Yu, Qing Huang
Summary: Glycine max, an economically important grain crop, has varied potential health benefits. Recent studies have focused on the bioavailability and bioaccessibility of soybean bioactive compounds. This review presents evidence-based information on the therapeutic and nutritional importance of soybean phytochemicals and their implications on general health. The bioavailability, metabolism, and safety of soybean phytochemicals are also discussed, as well as the need for clinical trials to confirm these functional properties in humans.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Spectroscopy
Guohua Yao, Qing Huang
Summary: Acetylation is a common and important protein modification. This study explored the vibrational assignments and geometric structures of acetylated lysines through Raman and infrared spectroscopy, providing guidance for detecting protein acetylation using spectroscopic techniques.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Chao Liu, Shenhao Wang, Xuan Dong, Qing Huang
Summary: This study proposes a simple and rapid method for in situ detection of pesticide residues on fruits and vegetables using surface-enhanced Raman spectroscopy (SERS). The flexible and transparent SERS substrates can be easily pasted onto the surface of agricultural products for residue detection. The experimental results show excellent detection performance and repeatability.
Review
Biochemistry & Molecular Biology
Mohammed Sharif Swallah, Precious Bondzie-Quaye, Yahui Wu, Adolf Acheampong, Frederick Leo Sossah, Shereen M. M. Elsherbiny, Qing Huang
Summary: Ganoderma lucidum (G. lucidum) is a traditional Asian medicine with a long history, known for its health-improving and life-extending properties. It contains bioactive metabolites such as polysaccharides, triterpenoids, and polyphenols, which have antioxidant, anti-inflammatory, anticancer, hepatoprotective, and antidiabetic activities. This mushroom species is widely used in the food industry as a nutrient supplement.
Article
Fisheries
Xinxin Zheng, Changsheng Shao, Jianxia Zhu, Leisheng Zhang, Qing Huang
Summary: Through experiments on normal cells, it was found that astaxanthin can significantly enhance the cell viability under gamma-ray radiation conditions by reducing reactive oxygen species (ROS) in the cells and alleviating radiation-induced DNA damage. Furthermore, astaxanthin was found to activate the Nrf2 signaling pathway and upregulate antioxidant proteins HO-1 and p-Nrf2, which helps alleviate oxidative stress caused by ionizing radiation. This study not only provides evidence of the outstanding antioxidant properties of astaxanthin, but also expands its potential applications in radiation protection.
AQUACULTURE INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Shereen M. Elsherbiny, Mahmoud A. Khalifa, Adolf Acheampong, Chao Liu, Precious Bondzie-Quaye, Mohammed S. Swallah, Xiuping Lin, Qing Huang
Summary: The potential of Bi2MoO6 nanoparticles as a photocatalyst for decomposing organic pollutants has been widely explored, but their potential for photodynamic therapy (PDT) has not been investigated. In this study, a novel nanocomposite based on Bi2MoO6/ MoS2/AuNRs was designed to overcome the limitation of UV absorption. The nanocomposite exhibited high photodynamic ability and POD-like activity under NIR-II light irradiation.
Review
Mycology
Precious Bondzie-Quaye, Mohammed Sharif Swallah, Adolf Acheampong, Shereen M. Elsherbiny, Edward Opong Acheampong, Qing Huang
Summary: Ganoderic acids (GA), secondary metabolites from Ganoderma lucidum, have diverse pharmacological activities and therapeutic effects. However, they are produced at low concentrations and require tedious cultivation. The elucidation of GA biosynthesis regulatory mechanisms has garnered interest, and strategies to control production and increase yield are explored. This review discusses insights into environmental and genetic regulation of GA biosynthesis and challenges in understanding GA diversification.
MYCOLOGICAL PROGRESS
(2023)
Article
Engineering, Environmental
Cao Fang, Changsheng Shao, Shenhao Wang, Yahui Wu, Chao Liu, Qing Huang
Summary: Antibiotics are widely used and their discharge into the environment poses a constant potential threat to the ecosystem. This study focused on the treatment of wastewater containing levofloxacin and sulfadiazine, two typical antibiotics, using atmospheric-pressure dielectric barrier discharge (DBD). The results showed that the total antibiotics removal efficiency could be improved compared to separate single antibiotic treatment. The role of plasma-induced reactive oxygen/nitrogen species (RONS) was examined, with hydroxyl radicals (OH) playing a major role in levofloxacin degradation and ozone and peroxynitrite also contributing to sulfadiazine degradation. The bio-toxicity evaluation demonstrated the safety of the DBD degradation products for the ecological environment.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Biochemistry & Molecular Biology
Mengmeng Duan, Yalin Li, Fengqiu Zhang, Qing Huang
Summary: This study induced the transformation of B-DNA to Z-DNA using NaCl, MgCl2, and ethanol, and monitored the transformation process using Fourier transform infrared (FTIR) spectroscopy. The structural changes were examined, and the DNA chirality alterations were validated by circular dichroism (CD) measurements. Z-DNA characteristic signals were observed in the IR spectrum, and the intensity ratios increased with Z-DNA content. Compared to CD spectra, IR spectra showed higher sensitivity to Z-DNA and provided more information about the molecular structure change. In conclusion, this study established a reliable FTIR analytical approach to assess BZ DNA conformational changes, which can help understand the Z-DNA transition mechanism and study Z-DNA functions in biological systems.
Review
Biotechnology & Applied Microbiology
Adolf Acheampong, Lamei Li, Shereen M. Elsherbiny, Yahui Wu, Mohammed Sharif Swallah, Precious Bondzie-Quaye, Qing Huang
Summary: This review investigates strategies to enhance Astaxanthin production from H. pluvialis, focusing on genetic and environmental factors and transcriptomic dynamics. Genetic engineering and culture optimization are effective approaches to increase Astaxanthin production, while new technologies may become principal strategies in the future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Guohua Yao, Chao Liu, Shereen M. Elsherbiny, Qing Huang
Summary: Ribose, the central unit in RNA, plays a crucial role in studying the origin of life and the chiral homogeneity of biological molecules. However, traditional chiral recognition methods for ribose are time-consuming, expensive, and limited to laboratory settings. In this study, a simple and convenient approach utilizing colorimetry and SERS was developed to quickly detect the chirality of ribose, providing a faster, cost-effective, and portable solution.
Article
Chemistry, Analytical
Fengqiu Zhang, Changsheng Shao, Yahui Wu, Wei Zhao, Xumiao Jing, Cao Fang, Qing Huang
Summary: In this study, a GFP fluorescent probe was utilized to construct a cell model for real-time observation of the dynamic change of glycolysis. It was found that TSA, an HDAC inhibitor, significantly enhanced glycolysis and inhibited cell proliferation. The mechanism of this process was associated with the regulation of HIF-1 alpha and c-Myc.
Article
Materials Science, Biomaterials
Shereen M. Elsherbiny, Changsheng Shao, Adolf Acheampong, Mahmoud A. Khalifa, Chao Liu, Qing Huang
Summary: This study demonstrates the potential of carbon quantum dots derived from broccoli as an effective photodynamic agent in Caenorhabditis elegans models. The carbon quantum dots showed excellent water solubility and optical properties, and exhibited strong photodynamic therapy efficiency by inducing germline apoptosis through the cep-1/p53 pathway.
BIOMATERIALS SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Muhammad Muhammad, Chang-Sheng Shao, Chao Liu, Qing Huang
Summary: The study developed a surface-enhanced Raman spectroscopy (SERS) assay for quantitative and targeted detection of exosomal miR-122 in mice after radiation treatments, showing increased expression of miR-122 postirradiation and the potential to assess drug-induced hepatic inflammation by detecting miR-122 using this method.
ACS APPLIED BIO MATERIALS
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
