Journal
TALANTA
Volume 207, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.120300
Keywords
Sodium alginate hydrogel; Metalloporphyrinic metal-organic framework nanosheets; Aptamer; Thrombin; Flow injection chemiluminescence
Categories
Funding
- Shandong Provincial Natural Science Foundation of China, China [ZR2016BM01]
- Horizontal Scientific Research Project of China [W15077]
- National Natural Science Foundation of China, China [21605094]
- Youth Science fund of Shandong Academy of Sciences, China [2017QN006]
- Shandong Province Natural Science Institute Joint Fund [ZR2015YL006]
Ask authors/readers for more resources
A signal-on chemiluminescence biosensor was established for detecting thrombin. The thrombin aptamer1-functionalized magnetic sodium alginate (Malg-Apt1) hydrogel was synthesized by physical interaction between sodium alginate and Ca2+, and it was used in the biosensor for separating and enriching thrombin. Ethylenediamine tetraacetic acid (EDTA) was used to chelate with Ca2+ to dissolve the hydrogel and release thrombin. A metalloporphyrinic metal-organic framework nanosheet, named as Cu-TCPP(Co) MOFs, was prepared as signal amplification strategy. Cu-TCPP(Co) MOFs/Au-ssDNA (ssDNA: single-strand DNA) was synthesized for controllable further amplification of chemiluminescent signal. The thrombin aptamer2-functionalized magnetic carbon nanotubes (MCNTs-Apt2) were used as a matrix, and Cu-TCPP(Co) MOFs/Au-ssDNA was adsorbed on the MCNTs by the complementary pairing of the partial bases between ssDNA and Apt2. Compared with ssDNA, Apt2 has a stronger interaction with thrombin. Therefore, thrombin can trigger the release of Cu-TCPP(Co) MOFs/Au-ssDNA to achieve signal amplification. Under the optimal conditions, the biosensor could detect thrombin as low as 2.178 x 10(-13) mol/L with the range from 8.934 x 10(-13) to 5.956 x 10(-10) mol/L and exhibited excellent selectively. Moreover, the signal-on chemiluminescence biosensor showed potential application for the detection of thrombin in body fluids.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available