Article
Chemistry, Analytical
Zhongjiao Feng, Jiasheng Wu, Meiyu Jiang, Jie Sha, Weimin Liu, Haohui Ren, Wenjun Zhang, Chun-Sing Lee, Pengfei Wang
Summary: The pH homeostasis of Golgi is crucial for maintaining normal cell functions. The development of an effective Golgi-targeting fluorescent probe (Golgi-hNR) that can sensitively detect pH changes is of great significance. By using Golgi-hNR, real-time tracking of Golgi pH variations in living cells has been successfully visualized.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Shumin Hou, Yong Wang, Yukun Zhang, Wenjing Wang, Xin Zhou
Summary: This study developed a fluorescent probe QG-S for quantifying cellular GSH and monitoring GSH dynamic variations in real time. The probe showed good performance in real-time monitoring of GSH dynamic variations and quantitatively determining GSH concentration in living cells.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Junxiong Yao, Qian Meng, Han Xu, Changlong Xu, Xiaohua Cao, Huanan Huang, Cuibing Bai, Rui Qiao
Summary: Real-time and accurate detection of intracellular GSH content is a challenge in the field of medicine and pathology. This study presents a novel fluorescent probe DPND with aggregation induced emission (AIE) characteristic for selective and quantitative detection of GSH. The probe shows low toxicity and good biocompatibility, making it a promising candidate for in vivo detection of GSH.
Article
Chemistry, Multidisciplinary
Haixian Ren, Fangjun Huo, Caixia Yin
Summary: Abnormal concentration of glutathione (GSH) is a risk factor for various diseases, and real-time monitoring of GSH levels is crucial for diagnosis and treatment. A dual-reversible-site probe with high selectivity for GSH has been developed for imaging intracellular GSH levels, providing insights into GSH-related events.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Applied
Sumita Subedi, Lok Nath Neupane, Pramod Kumar Mehta, Keun-Hyeung Lee
Summary: A new ratiometric fluorescent chemodosimeter for Hg2+ was developed based on an irreversible reaction of phenylboronic acid with Hg2+, showing selective and sensitive signal behavior to low concentrations of Hg2+ ions. The real-world application demonstrated quantification of Hg2+ ions in groundwater and tap water samples spiked with known amounts of Hg2+ ions, showcasing its potential environmental and biological sensing applications.
Article
Chemistry, Analytical
Xiaodi Rong, Caiyun Liu, Mingzhu Li, Hanchuang Zhu, Yan Zhang, Meijun Su, Xin Wang, Xiwei Li, Kun Wang, Miaohui Yu, Wenlong Sheng, Baocun Zhu
Summary: GSH concentration changes in Golgi are crucial for monitoring and treating tumor cells, making Golgi-targeted therapy a key strategy for effective cancer treatment.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zhoupeng Zheng, Shumin Feng, Shengyi Gong, Guoqiang Feng
Summary: Gol-CO is an effective fluorescence probe for imaging CO in Golgi apparatus, with high selectivity and sensitivity. It can target Golgi apparatus in living cells and zebrafish, and visualize the level increase of CO under oxidative stress.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Xin Zhang, Lin Chen, Ying-Ying Wei, Jing-Lei Du, Shi-Ping Yu, Xu-Guang Liu, Wei Liu, Yan-Jun Liu, Yong-Zhen Yang, Qiang Li
Summary: A fluorescence probe called GTCDs was developed for efficient imaging monitoring of Golgi apparatus. It targets the Golgi apparatus by forming hydrogen bonds with COX-2 on its surface, allowing for accurate visualization of Golgi apparatus' status.
Article
Chemistry, Analytical
Jingcan Qin, Hao Tian, Fei Kong, Yuanyuan Guo, Wenxian Du, Chuan Zhang, Hongmei Gu, Yuehua Li
Summary: Probes play a crucial role in the early diagnosis of tumors, especially tumor microenvironment-responsive probes. A GSH-activated near-infrared fluorescent (NIRF) and photoacoustic (PA) dual-modal probe exhibited significant signal enhancement and specificity, making it ideal for tumor-specific imaging.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Qian Zhang, Yonghe Tang, Sirui Song, Juanjuan Peng, Weiying Lin
Summary: A Golgi-targeted fluorescent probe (Gol-NTR) for nitroreductase (NTR) detection was developed, exhibiting good selectivity and low cytotoxicity. Gol-NTR was successfully applied for the first time to detect NTR in the Golgi apparatus.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhoupeng Zheng, Shengyi Gong, Jinzheng Zhang, Yijia Liu, Guoqiang Feng
Summary: A novel fluorescent probe for detecting Golgi NO level changes is reported, which has low cytotoxicity, high sensitivity, and selectivity. This probe can detect both endogenous and exogenous NO in cells and zebrafish, and can be used to study the rotenone-induced PD model.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Shaohui Han, Hui Zhang, Xiuxiu Yue, Jingpei Wang, Lei Yang, Benhua Wang, Xiangzhi Song
Summary: A ratiometric fluorescent probe CP was developed to selectively and rapidly discriminate between Cys and Hcy by introducing a phenylethynyl group. With distinct ratiometric fluorescence changes induced by the presence of Cys or Hcy, the probe was able to visualize and discriminate between intracellular Cys and Hcy in HeLa cells and zebrafish.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Shumin Feng, Zhoupeng Zheng, Shengyi Gong, Guoqiang Feng
Summary: This study reports a Golgi-targetable fluorescence probe for the detection of peroxynitrite. The probe exhibits low cytotoxicity, high sensitivity and selectivity, and displays unique targeting properties in living cells. The probe can be used to monitor peroxynitrite in the Golgi and evaluate drug-induced liver injury.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Environmental
Lin Wang, Yanyan Ma, Weiying Lin
Summary: In recent years, heavy metal mercury (II) pollutants have caused serious harm to human health and ecosystems. In this study, a novel fluorescent probe Coa-SH was designed and developed, which showed high selectivity for the detection of Hg2+ by utilizing the Hg2+-induced chemical reaction. The probe was proven to be effective in detecting mercury ions in aqueous solutions and in living organisms.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Chemistry, Applied
Feiran Liu, Xiaoli Zhang, Jing Jing, Xiaoling Zhang
Summary: Golgi stress is a major stress response in the Golgi apparatus that may disrupt redox balance and be related to various diseases. Hydrogen peroxide (H2O2), an important reactive oxygen species, plays a crucial role in the Golgi apparatus and abnormal concentrations of H2O2 can cause oxidative damage. On the other hand, hydrogen sulfide (H2S) acts as a protective agent to alleviate Golgi stress. A Golgi-targeted fluorescent probe, Golnap-H2O2, has been developed as a specific imaging tool for detecting H2O2 in the Golgi apparatus and as a potential H2S donor for cytoprotection.
Article
Medicine, Research & Experimental
Chunxia Chen, Shihai Yan, Shuang Qiu, Zhirong Geng, Zhilin Wang
Summary: The study demonstrated that FG4592 promotes the proliferation and migration of BMSCs, accelerating fracture healing. Additionally, HIF-1 alpha plays a crucial role in this process.
Article
Pharmacology & Pharmacy
Liu Shun, Wang Yi-xuan, Wang Hai-dan, Geng Zhi-rong, Yan Shi-hai
Summary: This study established a method to detect seven effective components in SKTM simultaneously using UHPLC-MS/MS, which was validated to have good repeatability and accuracy. This method may provide a new reliable approach for quality control of SKTM.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Taiyu Guo, Xinyue Chen, Wangbo Qu, Bin Yang, Ruowei Tian, Zhirong Geng, Zhilin Wang
Summary: A novel fluorescent probe, CySI, was developed to simultaneously distinguish cysteine and homocysteine with excellent selectivity and fast response. This probe could visualize exogenous Cys and Hcy in cells and monitor endogenous Cys fluctuations independently in mitochondria through the red emission channel.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Chunxia Chen, Shihai Yan, Zhirong Geng, Zhilin Wang
Summary: The HIF-1 alpha PHD inhibitor IOX2 improves the proliferation and migration of BMSCs by upregulating intracellular Ca2+ and decreasing ROS, thus promoting fracture healing.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Plant Sciences
Shihai Yan, Jiayin Chang, Xuehui Hao, Jiang Liu, Xiying Tan, Zhirong Geng, Zhilin Wang
Summary: The study found that berberine can alleviate the occurrence of colitis-associated colorectal tumors by altering the composition of intestinal flora. Berberine increases beneficial bacteria and levels of short-chain fatty acids, reducing inflammation and the risk of cancer.
Article
Multidisciplinary Sciences
Shi-Hai Yan, Li-Mu Hu, Xue-Hui Hao, Jiang Liu, Xi-Ying Tan, Zhi-Rong Geng, Jing Ma, Zhi-Lin Wang
Summary: This study revealed the direct binding target protein of berberine in colorectal cancer cells, which is pyruvate kinase isozyme type M2 (PKM2). Berberine inhibits the progression of colorectal cancer through hydrophobic interaction and pi-pi interaction, and it also inhibits the reprogramming of glucose metabolism.
Article
Chemistry, Applied
Taiyu Guo, Ruowei Tian, Wangbo Qu, Bin Yang, Zhirong Geng, Zhilin Wang
Summary: A mitochondria-targeted turn-on fluorescent sensor, CySH, with near-infrared absorption and emission wavelengths, was developed for the selective determination of copper, showing high selectivity and excellent anti-interference ability.
Article
Biochemistry & Molecular Biology
Tianxiang Li, Zhirong Geng, Ju Zhang, Lu Xu, Xiaoli Zhu
Summary: This study demonstrates the protective effects of pentapeptide bursopentin (BP5) against endotoxemia-induced acute lung injury. BP5 effectively alleviates lung injury by reducing oxidative stress and excessive inflammatory response through activation of the Nrf2 pathway. This mechanism involves the regulation of Keap1-Nrf2 interaction and Akt/GSK3 beta/Fyn pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Spectroscopy
Wangbo Qu, Taiyu Guo, Bin Yang, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: DFSN is a fluorescent probe with AIE and ESIPT, which can efficiently track hypochlorous acid in vivo. The probe has good selectivity and sensitivity, and can detect hypochlorous acid with the naked eye.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Wangbo Qu, Bin Yang, Taiyu Guo, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: In this study, a dual-response mitochondria-targeted fluorescent probe HBTN was developed for the quantitative detection of viscosity and HOCl. The probe showed promising results in both in vitro and in vivo experiments, providing a practical tool for monitoring viscosity and HOCl.
Editorial Material
Pharmacology & Pharmacy
Qiuwang Zhang, Shihai Yan, Dongdong Sun, Zhirong Geng, Peixiang Zhang
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Bin Yang, Ruowei Tian, Taiyu Guo, Wangbo Qu, Jiao Lu, Yong Li, Zhou Wu, Shihai Yan, Zhirong Geng, Zhilin Wang
Summary: This study reports the latest progress in using tetraphenylethylene (TPE) fluorophores as a tool for detecting intracellular adenosine triphosphate (ATP). Six different ATP probes were synthesized, and probes with dual positive charge sites showed better selectivity and sensitivity. Among them, P2, with a D-π-A structure, 1,4-bis(bromomethyl)benzene linker, and dual positive charge recognition sites, exhibited higher sensitivity and selectivity in ATP detection. This research has important application value in monitoring the fluctuations of mitochondrial ATP levels.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Hong Huang, Junyao Xu, Siqi Zhang, Jing Zhao, Shun Liu, Lei Tian, Haidan Wang, Zhirong Geng, Shihai Yan
Summary: This study used network pharmacology combined with UHPLC-MS/MS, molecular docking, and in vivo validation to investigate the active components and therapeutic mechanisms of SKTMG in the treatment of heart failure. The results identified 192 active compounds and 307 potential consensus targets for SKTMG, and discovered 10 core target genes related to the MAPK signal pathway. Additionally, SKTMG components were found to bind to specific targets and inhibit phosphorylation, leading to improved CHF in rats.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Hai-Li Yang, Li-Fei Bai, Zhi-Rong Geng, Hao Chen, Le-Tian Xu, Yang-Cun Xie, Dong-Jin Wang, Hong-Wei Gu, Xiao-Ming Wang
Summary: As an excellent fluorescent nanomaterial, carbon quantum dots (CQDs) possess various properties that make them suitable for biomedical applications. This review provides an overview of the synthesis methods, optical properties, and biomedical applications of CQDs, including detection, imaging, and drug delivery. The review also discusses the challenges and future prospects of CQDs in the biomedical sector.
MATERIALS TODAY ADVANCES
(2023)
Article
Chemistry, Applied
Bin Yang, Wangbo Qu, Taiyu Guo, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: A tetraphenylethylene fluorophore based on D-pi-A structure was used to design and synthesize the TPE-PyFP probe for selective detection of ATP. TPE-Py-FP showed a turn-on response after adding ATP and had a wide linear range and low limit of detection. The detection mechanism was mainly the electrostatic interaction between the positively charged sites of TPE-Py-FP and the negatively charged triphosphate structure of ATP.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).