Journal
TALANTA
Volume 205, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.120131
Keywords
Fluorescence; Aptamer; Carbon dots; Humic acid; Quenching; Aflatoxin
Categories
Funding
- Natural Science Foundation of Shandong Province, China [ZR2019MD025]
- 13th Five -Year National Key Development Program of China [2016YFD0401403, 2018YFD0401103]
- National Natural Science Foundation of China [41771342, 31872889]
- Funds of Shandong Double Tops Program [SYL2017XTTD01, SYL2017XTTD15]
- Key R&D programs in Shandong [2018YYSP029]
- Dodd -Walls Centre for Photonic and Quantum Technologies
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This work described a fluorometric and aptamer-based assay for aflatoxin B1 (AFB1). Aptamer-modified carbon dots (DNA-CDs) were first synthesized as fluorescence probes, then reacted with humic acid (HAs) which acted as quencher of the blue fluorescence of the CDs. It was found that HAs can readily adsorb ssDNA aptamers due to the presence of a rich surface chemistry (quinoidal units, aromatic rings and sugar moieties). This resulted in quenching of the fluorescence of the CDs (with excitation/emission peaks at 360/450 nm), probably due to pi interactions. If the nanoprobe was reacted with AFB1, the DNA-CDs detached from the HAs and fluorescence was restored. Under optimized experimental conditions, the assay had a linear response in the 0.1-0.8 ng mL(-1) AFB1 concentration range, with a low limit of detection of 70 pg mL(-1).
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