Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 19-24Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.026
Keywords
Electrochemical immunosensor; Biosensor; Screen-printed carbon electrode; Allergen; Peanut; Ara h 1
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) - POPH-QREN-Tipologia 4.1-Formacao Avancada [SFRH/BPD/68883/2010]
- European Union (FEDER funds through COMPETE)
- National Funds (FCT) [Pest-C/EQB/LA0006/2013]
- European Union (FEDER funds) [NORTE-07-0124-FEDER-000069]
- Fundo Social Europeu
- Ministerio da Ciencia, Tecnologia e Ensino Superior
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A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery > 96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut. (C) 2014 Elsevier B.V. All rights reserved.
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