Review
Biochemical Research Methods
Emiko Kinoshita-Kikuta, Tohru Koike, Eiji Kinoshita
Summary: The Phos-tag technique is suitable for quantitative analysis of His- and Asp-phosphorylated proteins in a bacterial two-component system. This technique allows monitoring of auto-phosphorylation reactions of HK and RR, as well as phosphotransfer reactions from HK to RR. Additionally, the technique can be used for profiling potential HK inhibitors, providing a simple and convenient approach for drug discovery targeting the bacterial TCS.
JOURNAL OF PROTEOMICS
(2022)
Article
Chemistry, Analytical
Yanyan Niu, Yuxue Chen, Xiaoping Zhang, Hui Xie, Guiling Luo, Wei Sun
Summary: This study developed a PEC aptasensor for quantitative analysis of Cd(II) by changing the sensing interface structure to amplify the detection signal. The sensor showed high sensitivity with a detection limit of 1.1 x 10-11 mol/L and was successfully used for water sample detection.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Yasunori Sugiyama, Yuuki Uezato
Summary: Protein kinases play crucial roles in regulating biological processes and disease pathogenesis. Investigating the phosphorylation status of protein kinases is essential for understanding biological phenomena and disease mechanisms. Phos-tag, a tool for studying protein phosphorylation, along with techniques like Phos-tag SDS-PAGE, has been developed for analyzing protein phosphorylation.
JOURNAL OF PROTEOMICS
(2022)
Review
Chemistry, Analytical
Sze Shin Low, Zetao Chen, Yaru Li, Yanli Lu, Qingjun Liu
Summary: This review provides a comprehensive overview of the field of photoelectrochemical biosensing, with a focus on the design principles and synthesis methods of g-C3N4-based PEC biosensors. The review highlights the three design principles of g-C3N4-based PEC biosensors, providing researchers with comprehensive design guidelines.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Harunori Yoshikawa, Kohei Nishino, Hidetaka Kosako
Summary: In this study, novel ERK substrates were identified using a phosphoproteomic approach, and the phosphorylation of these substrates by active ERK was confirmed in both cellular and in vitro experiments. The study demonstrates the usefulness of Phos-tag SDS-PAGE for validating candidate substrates of protein kinases.
JOURNAL OF PROTEOMICS
(2022)
Review
Chemistry, Inorganic & Nuclear
Yunlei Zhou, Huanshun Yin, Shiyun Ai
Summary: The photoelectrochemical (PEC) assay is an emerging innovative detection technique that has played a significant role in advancing analytical methods. Two-dimensional layered nanomaterials, including various semiconductors, have garnered considerable attention for their excellent performance in PEC sensing and biosensing applications. The future prospects for the application of 2D layered nanomaterials in PEC technology are promising.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Analytical
Cuiping Fu, Xuefeng Wang, Fumin Xue, Peihua Zhu, Weijia Zhou, Shenguang Ge, Jinghua Yu
Summary: A photoelectrochemical biosensor based on a multiple signal amplification strategy was developed for highly sensitive detection of microRNA. The sensor utilized TiO2 and CuInS2-sensitized TiO2 layers to enhance photoelectrical properties and achieve initial signal amplification. Signal amplification was further carried out through an enzyme-free hybridization reaction. The sensor exhibited excellent linear response, with a wide detection range and low detection limit. Clinical biomolecular monitoring of miRNA using this sensor demonstrated excellent quantitative detection capability.
Article
Chemistry, Analytical
Takanobu Nobori, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama
Summary: The translated text describes a novel FRET-based protein kinase activity monitoring method using quantum dots and fluorophore-labeled substrate peptides. By utilizing phos-tag to capture phosphate groups and induce FRET, the system successfully measures protein kinase activity, demonstrating the feasibility of the assay.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Burcak Demirbakan, Mustafa Kemal Sezginturk
Summary: A novel electrochemical immunosensor has been developed for the diagnosis of cardiovascular disease. The immunosensor exhibits a wide detection range and low detection limit, making it suitable for clinical applications.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ying Qin, Jingyi Zhang, Rong Tan, Zhichao Wu, Mingwang Liu, Jinli Li, Miao Xu, Wenling Gu, Chengzhou Zhu, Liuyong Hu
Summary: In this study, an in situ-sensitized photoelectrochemical biosensor using a small-molecule probe was developed for accurate detection of a-glucosidase activity. The in situ sensitization approach enhanced the light-harvesting property and photoexcited carrier separation efficiency, resulting in excellent accuracy, a low detection limit, and outstanding anti-interference ability.
Article
Chemistry, Analytical
Meng-Jie Li, Si-Yu An, Ying Wu
Summary: A sensitive photoelectrochemical biosensing platform was developed for quantitative monitoring of miRNA-141 using Au nanoparticles@graphitic-like carbon nitride as the signal generator and T7 exonuclease-involved target cycle amplification. The biosensor showed gradually inhibited PEC signal with increasing miRNA-141 concentration in the range from 1 fM to 1 nM, with a detection limit of 0.3 fM, providing a valuable scheme for early disease diagnosis and biological research.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Emiko Kinoshita-Kikuta, Momoka Yoshimoto, Marina Yano, Eiji Kinoshita, Tohru Koike
Summary: This article discusses an assay of tyrosine protein kinase ABL activity using an Escherichia coli protein expression system. The study involves co-expressing human tyrosine kinase ABL and its substrate in E. coli and detecting tyrosine phosphorylation using Phos-tag SDS-PAGE. Results show different kinase activity levels in mutant forms of ABL compared to the wild-type, indicating a correlation between phosphorylation states and enzymatic activity.
Article
Chemistry, Physical
Xue Zhang, Chuanjun Xi, Yuanhang Yue, Puhui Deng, Linping Zhang, Yu Hou
Summary: By modifying g-C3N4 with coral-like NiSe2, a composite photocatalyst with high efficiency was prepared, which greatly enhances the photocatalytic performance of g-C3N4 and achieves efficient photocatalytic hydrogen generation.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Biophysics
Mingjun Xiao, Minghui Zhu, Ruo Yuan, Yali Yuan
Summary: In this work, an ultrasensitive PEC biosensor based on dual-sensitized PDA/ZnO@MoS2 QDs photoactive material was proposed for detection of miRNA-182-5p. The DSN-assisted target recycling amplification triggered a multilocus domino-like DNA cascade reaction on DNA nanonet, which significantly enhanced the detection sensitivity. The developed biosensor exhibited a low detection limit of 0.17 fM, providing a new avenue for sensitive, fast and reliable sensing of various DNA/RNA.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Chemical
Li Yan, Jingyang Hou, Tiantian Li, Yanbo Wang, Chunbo Liu, Tianyu Zhou, Wei Jiang, Dandan Wang, Guangbo Che
Summary: An integrated CN photocatalyst with enhanced photocatalytic performance was successfully fabricated through thermal-induced copolymerization of PEI precursor with urea. The optimized photocatalyst showed efficient degradation of tetracycline and hydrogen production under visible light, providing a reference for developing simple, low-cost, and environmentally friendly CN photocatalysts to address energy crisis and environmental problems.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Correction
Biophysics
Min-hui Zhao, Lin Cui, Bing Sun, Quanbo Wang, Chun-yang Zhang
BIOSENSORS & BIOELECTRONICS
(2020)
Correction
Biophysics
Bing Sun, Jing Dong, Lin Cui, Tiantian Feng, Jiujuan Zhu, Xuanhe Liu, Shiyun Ai
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Yufei Wang, Tianyou Chen, Chunxia Huang, Yanran Wang, Jing Wu, Bing Sun
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Xuan-He Liu, Ya-Wen Yang, Xiao-Ming Liu, Qing Hao, Li-Mei Wang, Bing Sun, Jing Wu, Dong Wang
Article
Thermodynamics
Jiujuan Zhu, Hanhan Fan, Bing Sun, Hongbin Qi
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2020)
Article
Acoustics
Xiaoming Liu, Mulin Zhuo, Wendi Zhang, Man Gao, Xuan-He Liu, Bing Sun, Jing Wu
ULTRASONICS SONOCHEMISTRY
(2020)
Article
Chemistry, Physical
Jiujuan Zhu, Hongbin Qi, Bing Sun, Hanhan Fan, Xiaoyun Zhang
JOURNAL OF MOLECULAR LIQUIDS
(2020)
Article
Chemistry, Multidisciplinary
Xindong Wang, Xinyu Wang, Bo Qu, Nuernisha Alifu, Ji Qi, Ruiyuan Liu, Qinrui Fu, Ruifang Shen, Qi Xia, Lijun Wu, Bing Sun, Jibin Song, Youping Lin, Xin Huang, Anjun Qin, Jun Qian, Ben Zhong Tang, Guanying Chen
Summary: This study introduces a class of biocompatible dye-protein optical nanoprobes with long-term photostability, superbrightness, and enriched functional groups, capable of producing stable and bright fluorescence for various applications in biological imaging.
Article
Chemistry, Physical
Tiantian Feng, Daniel Streater, Bing Sun, Korlan Duisenova, Dong Wang, Yi Liu, Jier Huang, Jian Zhang
Summary: In this study, the photophysical properties of three highly crystalline imine-linked 2D COFs were systematically investigated using experimental and computational methods. It was found that the different electron affinities in the materials can influence the excited state, charge transfer direction, and lifetime, thus affecting the performance of the materials. One of the materials exhibited a greater degree of delocalization, resulting in a long-lived charge-separated state.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Chongqing Yang, Kaiyue Jiang, Qi Zheng, Xinle Li, Haiyan Mao, Wenkai Zhong, Cheng Chen, Bing Sun, Haimei Zheng, Xiaodong Zhuang, Jeffrey A. Reimer, Yi Liu, Jian Zhang
Summary: This study reports the synthesis of COFs with efficient charge transport and chemical stability, as well as the growth of thin films on various substrates. The thin films exhibit excellent semiconducting properties and significant increases in conductivity. Additionally, density functional theory calculations reveal the anisotropic nature of carrier transport in the framework.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Tianyou Chen, Yufei Wang, Saiyu Zhang, Zhen Gao, Dajun Zhao, Jing Wu, Kun Shen, Bing Sun
Summary: In this study, a novel photoactive material, Ti3C2Tx/AgI heterojunction material (HM), was synthesized for cathodic photoelectrochemical (PEC) sensing. The Ti3C2Tx/AgI HM exhibited enhanced electron-hole separation and charge transport, leading to excellent PEC performance. A cathodic PEC sensor was developed for glutathione (GSH) detection, showing high selectivity and stability.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Physical
Tiantian Feng, Qing Hao, Bing Sun, Dong Wang
Summary: Metal-covalent organic frameworks (MCOFs) with regular structures, adjustable pore channels, designable structures, and abundant tunable metal-catalytic active sites are promising candidates for advanced single-atom enzymes.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Tiantian Feng, Bing Sun, Qing Hao, Jing Li, Ying Xu, Hong Shang, Dong Wang
Summary: Highly crystalline Fe-COFs were successfully constructed using a subcomponent assembly strategy. The spectral results confirm the coordination of Fe(ii) ions with the nitrogen atoms of the iminopyridine linkages.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Yu Gu, Chunhua Li, Yingbin Wang, Wenxin Lu, Hong Shang, Bing Sun
Summary: Precisely constructing lithiophilic active sites on the anode surface is an effective strategy for suppressing the dendrite growth of lithium in Li-metal batteries. In this study, a phthalocyanine polymer with lithiophilic active sites was designed and applied on the copper foil surface, successfully inhibiting the dendrite growth through homogeneous nucleation and deposition processes. The phthalocyanine polymer exhibited reduced overpotential, high coulombic efficiency, and prolonged lifespan.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Chunhua Li, Yu Gu, Yingbin Wang, Bing Sun, Hong Shang
Summary: This study demonstrates the preparation of a lithiophilic two-dimensional porous conjugated porphyrin polymer on copper foil for achieving uniform lithium deposition, leading to improved performance of high-energy density lithium metal batteries. By designing precise lithiophilic sites, it is possible to decrease nucleation overpotential, suppress dendritic growth, and enhance electrochemical performance of the electrode.
DALTON TRANSACTIONS
(2021)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)