Article
Biophysics
Silio Lima Moura, Arnau Pallares-Rusinol, Luciano Sappia, Merce Marti, Maria Isabel Pividori
Summary: This article introduces a biosensor combining immunomagnetic separation and electrochemical biosensing based on the intrinsic ALP activity of exosomes. By improving the analytical performance for detecting ALP activity in exosomes, reliable differentiation between breast cancer patients and healthy individuals is achieved.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Oncology
Jingjing Yu, Arsha Ostowari, Amber Gonda, Kiarash Mashayekhi, Farshid Dayyani, Christopher C. W. Hughes, Maheswari Senthil
Summary: Exosomes are small extracellular vesicles that carry important information for intercellular communication. They are produced abundantly by tumor cells and carry tumor-specific content, such as DNA, RNA, and proteins, which play significant roles in cancer development and progression. Due to their presence in various biofluids, exosomes can be developed as liquid biopsy tools for early diagnosis, molecular profiling, disease surveillance, and treatment response monitoring. This review focuses on the diagnostic, prognostic, and predictive potential of exosomal biomarkers in colorectal and gastric cancers, and discusses the challenges and limitations of their clinical application.
Article
Immunology
Bingduo Wang, Aliona Woehler, Johannes Greven, Rebekka J. S. Salzmann, Cindy M. Keller, Tobias Tertel, Qun Zhao, Uemit Mert, Klemens Horst, Ludmila Lupu, Markus Huber-Lang, Martijn van Griensven, Tom Erik Mollnes, Sebastian Schaaf, Robert Schwab, Christian P. Strassburg, Ingo G. H. Schmidt-Wolf, Bernd Giebel, Frank Hildebrand, Veronika Lukacs-Kornek, Arnulf G. Willms, Miroslaw T. Kornek
Summary: This study investigated the potential of serum-derived extracellular vesicles (EVs) as biomarkers for decision-making in polytrauma. The findings suggest that specific types of small EVs may serve as valuable, non-invasive, and objective biomarkers for assessing and monitoring the severity of polytrauma and associated organ damage.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Amaia Sandua, Estibaliz Alegre, Alvaro Gonzalez
Summary: Lung cancer is a major cause of cancer-related deaths globally, with late detection and frequent treatment resistance being common issues. Exosomes, as microvesicles secreted by cells, especially tumor cells, play a crucial role in lung cancer development and can be utilized for diagnosis, prognosis, and follow-up. However, the isolation and analysis of exosomes can be challenging, yet their cargo proteins, RNA, and DNA provide valuable insights into lung cancer progression and management.
Review
Medicine, Research & Experimental
Kai Zhao, Xiangyu Li, Yuanxin Shi, Yun Lu, Peng Qiu, Zhengdong Deng, Wei Yao, Jianming Wang
Summary: Cholangiocarcinoma is an aggressive malignancy with a high fatality rate. Exosomes, as communication messengers, play important roles in tumor microenvironment and have potential value in the diagnosis and treatment of cholangiocarcinoma.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Laura Benecke, Mali Coray, Sandra Umbricht, Dapi Chiang, Fabricio Figueiro, Laurent Muller
Summary: Glioblastomas, highly aggressive tumors with low survival rates, create an immunosuppressive microenvironment through the transportation of biomolecules by exosomes. Recent studies have shown the significant impact of tumor-derived exosomes on immune cells, highlighting their potential as diagnostic tools and therapeutic targets for aggressive tumor cells like glioblastomas. Exosomes serve as important instruments for molecular understanding of tumors given their structural and functional properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Maria Lamminaho, Jouni Kujala, Hanna Peltonen, Maria Tengstrom, Veli-Matti Kosma, Arto Mannermaa
Summary: The study found that high cfDI is an independent prognostic factor for poor overall survival and breast cancer-specific survival in breast cancer cases, and its inclusion in logistic regression models improves predictive performance, suggesting its prognostic potential.
Review
Biochemistry & Molecular Biology
Aleksei Shefer, Alena Yalovaya, Svetlana Tamkovich
Summary: Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in women. Understanding the mechanisms behind tumor progression is crucial. Exosomes, which are vesicles that mediate communication between cells, have been found to play a significant role in breast cancer pathogenesis. Recent studies have shown that tumor-derived exosomes can activate multiple signaling pathways and promote tumor development through the transfer of biologically active molecules. This review explores the involvement of exosomes in breast cancer and discusses their potential as diagnostic biomarkers for liquid biopsy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Artur Slomka, Bingduo Wang, Tudor Mocan, Adelina Horhat, Arnulf G. Willms, Ingo G. H. Schmidt-Wolf, Christian P. Strassburg, Maria A. Gonzalez-Carmona, Veronika Lukacs-Kornek, Miroslaw T. Kornek
Summary: Liquid biopsies, as biomarkers in cancer diagnosis, have great potential but also limitations. This review focuses on comparing extracellular vesicles (EVs) and circulating tumor cells (CTCs) and discussing their similarities in characterization and quantification. While it is not concluded which is better between CTCs and EVs, the review provides reasons to intensify research on both.
Review
Chemistry, Physical
Bingqian Lin, Yanmei Lei, Junxia Wang, Lin Zhu, Yuqi Wu, Huimin Zhang, Lingling Wu, Peng Zhang, Chaoyong Yang
Summary: Liquid biopsy based on exosomes provides valuable information for biomarker analysis, but faces challenges in exosome isolation and molecular detection. The advancement of microfluidics technology has significantly improved the selectivity and sensitivity of exosome capture and molecular detection.
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Review
Oncology
Xiangrong Liu, Dimitri Papukashvili, Zhixiang Wang, Yan Liu, Xiaoxia Chen, Jianrong Li, Zhiyuan Li, Linjie Hu, Zheng Li, Nino Rcheulishvili, Xiaoqing Lu, Jinfeng Ma
Summary: Breast cancer is the most common malignancy in women, and traditional cancer detection methods are insufficient in detecting the complete cancer genomic landscape, necessitating the development of new strategies. Liquid biopsy has gained attention for its simplicity, early detection, and effective monitoring, with miRNAs potentially playing a crucial role in the diagnosis and prognosis of breast cancer.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Samuel Amintas, Veronique Vendrely, Charles Dupin, Louis Buscail, Christophe Laurent, Barbara Bournet, Jean-Philippe Merlio, Aurelie Bedel, Francois Moreau-Gaudry, Julian Boutin, Sandrine Dabernat, Etienne Buscail
Summary: Extracellular vesicles (EVs) are small vesicles produced by healthy tissues and tumor cells, carrying a rich content of biomolecules, which play important roles in cancer control and progression. They are being studied as carriers of liquid biopsy biomarkers, with double-stranded DNA (dsDNA) in EVs showing potential as a promising biomarker for tumor presence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Francisco Gabriel Ortega, German E. Gomez, Coral Gonzalez-Martinez, Teresa Valero, Jose Exposito-Hernandez, Ignacio Puche, Alba Rodriguez-Martinez, Maria Jose Serrano, Jose Antonio Lorente, Martin A. Fernandez-Baldo
Summary: Prostate cancer is the most common malignancy in males, and early diagnosis is crucial for better prognosis. We have developed a portable platform for quantifying prostate-specific antigen (PSA) that can be read on a smartphone. This technique is fast, easy to use, specific, sensitive, and reproducible, making it a promising tool for healthcare applications.
Review
Oncology
Shiyu Li, Ming Yi, Bing Dong, Ximin Tan, Suxia Luo, Kongming Wu
Summary: Exosomes, as a new type of liquid biopsy, have the potential to be diagnostic or prognostic biomarkers by delivering cargos including nucleic acids and protein, involving in tumorigenesis, tumor growth, metastasis, and drug resistance.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Multidisciplinary Sciences
Patricia de Maria Silva Figueiredo, Jose Costa Sampaio Filho, Alzirene de Jesus Sales Sodre, Jose Ribamar de Castro Junior, Ingrid Santos Goncalves, Rodrigo Vieira Blasques, Rodrigo S. Correa, Benedicto Augusto Vieira Lima, Larissa dos Anjos Marques, Denise Fernandes Coutinho, Ana Paula Silva de Azevedo dos Santos, Tassio Romulo Silva Araujo Luz, Rita de Cassia Mendonca de Miranda, Julliana Ribeiro Alves dos Santos, Antonio Carlos Doriguetto, Maria Isabel Pividori, Manfredo Horner, Paulo Cesar Mendes Villis
Summary: In this study, novel triazene compounds were synthesized and tested for their antimicrobial and antifungal activities. The results demonstrated the potential of triazenes as agents against resistant bacterial and fungal strains, with promising activities observed against both gram-positive and gram-negative bacteria, as well as various fungi.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Angela Quintana, Vicente Peg, Aleix Prat, Teresa Moline, Guillermo Villacampa, Laia Pare, Patricia Galvan, Rodrigo Dientsmann, Peter Schmid, Giuseppe Curigliano, Eva Munoz-Couselo, Jose Perez-Garcia, Merce Marti, Juan Blanco-Heredia, Carla Dos Anjos, Miguel Vazquez, Leticia De Mattos-Arruda, Javier Cortes
Summary: Only a small percentage of TNBC patients have high levels of TILs, while another portion lacks TILs. The low levels of TILs at diagnosis may be due to lymphocytes being deactivated by immune checkpoints in local lymph nodes, preventing migration to the tumor.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Chemistry, Multidisciplinary
Juan Carlos Porras, Mireia Bernuz, Jennifer Marfa, Arnau Pallares-Rusinol, Merce Marti, Maria Isabel Pividori
Summary: Lateral flow assay (LFA) is a paper-based test designed for detecting specific analytes in low-resource settings. While colloidal gold is commonly used for detection, carbon nanoparticles (CNPs) have shown to be more sensitive, cost-effective, and stable, making them promising alternatives for improving the detection technology.
Article
Biophysics
Silio Lima Moura, Arnau Pallares-Rusinol, Luciano Sappia, Merce Marti, Maria Isabel Pividori
Summary: This article introduces a biosensor combining immunomagnetic separation and electrochemical biosensing based on the intrinsic ALP activity of exosomes. By improving the analytical performance for detecting ALP activity in exosomes, reliable differentiation between breast cancer patients and healthy individuals is achieved.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiaoqian Tang, Qi Zhang, Maria Isabel Pividori, Zhaowei Zhang, Jean-Louis Marty, Gaelle Catanante
Summary: Patulin contamination is a serious health risk factor for food safety and human health. In this study, a sensitive aptamer-based lateral flow assay was developed for rapid and simple patulin detection in apple juice.
Review
Oncology
Andrea Aran, Laia Garrigos, Giuseppe Curigliano, Javier Cortes, Merce Marti
Summary: The study of TCR repertoire in cancer patients is important for understanding the anti-tumoural response, predicting prognosis, and determining response to immune checkpoint inhibitor therapies. The TCR repertoire is influenced by various factors and can provide valuable insights for modulating immune responses and predicting outcomes.
Article
Chemistry, Analytical
Arnau Pallares-Rusinol, Silio Lima Moura, Merce Marti, Maria Isabel Pividori
Summary: This study developed a new method for the sensitive detection of cancer by preconcentrating exosomes released by tumor cells from serum using immunomagnetic separation and measuring GAPDH transcripts in exosomes using reverse transcription polymerase chain reaction and electrochemical genosensing. The application of this method in breast cancer cells and human serum samples showed high sensitivity and specificity, suggesting it as a promising strategy for minimal invasive liquid biopsy.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Amanda Cano, Miren Ettcheto, Mireia Bernuz, Raquel Puerta, Ester Esteban de Antonio, Elena Sanchez-Lopez, Eliana B. Souto, Antonio Camins, Merce Marti, Maria Isabel Pividori, Merce Boada, Agustin Ruiz
Summary: Extracellular vesicles are secreted by cells and play important roles in intercellular communication, immune responses, human reproduction, and synaptic plasticity. They have potential as biomarkers for diseases due to their biological relevance and easy accessibility. They can also be used as mirrors of brain diseases as they can cross the blood-brain barrier. This review explores their biogenesis, functions, and potential in clinical practice, as well as their role in major human brain diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Amanda Cano, Ester Esteban-de-Antonio, Mireia Bernuz, Raquel Puerta, Pablo Garcia-Gonzalez, Itziar de Rojas, Claudia Olive, Alba Perez-Cordon, Laura Montrreal, Raul Nunez-Llaves, Oscar Sotolongo-Grau, Emilio Alarcon-Martin, Sergi Valero, Montserrat Alegret, Elvira Martin, Pamela V. Martino-Adami, Miren Ettcheto, Antonio Camins, Assumpta Vivas, Marta Gomez-Chiari, Miguel angel Tejero, Adelina Orellana, Lluis Tarraga, Marta Marquie, Alfredo Ramirez, Merce Marti, Maria Isabel Pividori, Merce Boada, Agustin Ruiz
Summary: Alzheimer's disease (AD) is commonly preceded by a prodromal phase characterized by high levels of A beta and p-tau-mediated neuronal injury and mild cognitive impairment (MCI). Differential diagnosis of MCI causes in these prodromal stages is a challenge for clinicians. Blood biomarkers, particularly plasma extracellular vesicles (pEVs), are emerging as potential tools for early AD detection.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Immunology
Angela Quintana, Enrique Javier Arenas, Cristina Bernado, Jose Fernandez Navarro, Jonatan Gonzalez, Anna Esteve-Codina, Teresa Moline, Merce Marti, Giuseppe Curigliano, Peter Schmid, Vicente Peg, Joaquin Arribas, Javier Cortes
Summary: The heterogeneity of immune infiltration in breast cancer is an important research area. This study found that the heterogeneity of TILs is related to the presence of immune infiltration, rather than patient characteristics.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Mengyuan Li, Angela Quintana, Elena Alberts, Miu Shing Hung, Victoire Boulat, Merce Marti Ripoll, Anita Grigoriadis
Article
Immunology
Andrea Aran, Gonzalo Lazaro, Vicente Marco, Elisa Molina, Ferran Abanco, Vicente Peg, Maria Gion, Laia Garrigos, Jose Perez-Garcia, Javier Cortes, Merce Marti
Summary: Tumor-infiltrating lymphocytes (TILs) have predictive and prognostic value in breast cancer. TCR differences between CD4+ and CD8+ TILs were observed, and a more restricted TCR repertoire with higher similarity was found in CD4+ TILs.
FRONTIERS IN IMMUNOLOGY
(2023)