Synthesis of low immunogenicity RNA with high-temperature in vitro transcription

RNA Modifications Modulate Activation of Innate Toll-Like Receptors

(2019) Isabel Freund et al.   Genes

A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA

(2019) Markus Baiersdörfer et al.   Molecular Therapy-Nucleic Acids

PKR activation by non-canonical ligands: a 5′-triphosphate requirement versus antisense contamination.

(2019) Sarah A Safran et al.   RNA

Efficient inhibition of RNA self-primed extension by addition of competing 3′-capture DNA-improved RNA synthesis by T7 RNA polymerase

(2019) Yasaman Gholamalipour et al.   NUCLEIC ACIDS RESEARCH

An origin of the immunogenicity of in vitro transcribed RNA

(2018) Xin Mu et al.   NUCLEIC ACIDS RESEARCH

Large-Scale in Vitro Transcription, RNA Purification and Chemical Probing Analysis

(2018) Fariha Kanwal et al.   CELLULAR PHYSIOLOGY AND BIOCHEMISTRY

3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses

(2018) Yasaman Gholamalipour et al.   NUCLEIC ACIDS RESEARCH

Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge

(2017) Norbert Pardi et al.   Nature Communications

RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling

(2016) Ann Fiegen Durbin et al.   mBio

Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5′-diphosphates

(2014) Delphine Goubau et al.   NATURE

mRNA-based therapeutics — developing a new class of drugs

(2014) Ugur Sahin et al.   NATURE REVIEWS DRUG DISCOVERY

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

(2011) Katalin Karikó et al.   NUCLEIC ACIDS RESEARCH

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY