Article
Chemistry, Analytical
Ariany Soares Coco, Fabiana Vasconcelos Campos, Camilo Arturo Rodriguez Diaz, Marco Cesar Cunegundes Guimaraes, Adilson Ribeiro Prado, Jairo Pinto de Oliveira
Summary: In this study, a biosensor utilizing the localized surface plasmon resonance (LSPR) of gold nanoparticles (AuNPs) was developed to detect glyphosate in food samples. AuNPs were synthesized, functionalized with either cysteamine or anti-glyphosate antibody, and characterized using various techniques. Both conjugates successfully detected the presence of glyphosate, with the antibody functionalized AuNPs exhibiting broader detection range and better performance. This study demonstrates the potential of AuNP-based biosensors for glyphosate detection in food samples.
Article
Chemistry, Multidisciplinary
Angeline S. Dileseigres, Yoann Prado, Olivier Pluchery
Summary: The functionalization of gold nanoparticles with thiol molecules in solution is crucial for their application development. In this study, the changes in localized surface plasmon resonance (LSPR) peak due to the reaction between thiol molecules and gold nanoparticles were investigated using UV-visible spectroscopy. The saturation of thiol molecules on the nanoparticle surface was confirmed, and the wavelength shift was accurately predicted using an analytical model. The kinetics of the functionalization reaction were found to be rapid, completing in less than an hour.
Article
Nanoscience & Nanotechnology
Chia-Wen Kuo, Sheng-Hann Wang, Shu-Cheng Lo, Wei-Han Yong, Ya-Lun Ho, Jean-Jacques Delaunay, Wan-Shao Tsai, Pei-Kuen Wei
Summary: The study proposes a method for sensitive detection of small molecules by coupling image dipoles of gold nanoparticles with Fano resonance of capped gold nanoslits. The coupling mechanism was verified through calculations and experiments, showing potential for detecting low concentrations of small molecules. This resonant coupling significantly pushes the sensitivity boundary, with the detection limit reported to be 3 orders of magnitude lower than prism-based SPR.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Vivek Semwal, Oliver Rishoj Jensen, Ole Bang, Jakob Janting
Summary: This paper presents numerical and experimental results on the effects of spherical gold nanoparticles (AuNPs) size on Localized Surface Plasmon Resonance (LSPR) refractive index (RI) sensitivity, Figure of Merit (FoM), and penetration depth (dp). The study also investigates the influence of AuNP dimer interparticle distance (ds) on these parameters. The results show that a diameter of 60 nm AuNPs provides the best FoM. Furthermore, the interparticle distance between d = 20 nm and 60 nm AuNPs significantly affects the RI sensitivity. The importance of penetration depth information for biosensing using aptamers is discussed in relation to aptamer size.
Article
Chemistry, Analytical
Yaliang Huang, Ting Sun, Lin Liu, Ning Xia, Yuehua Zhao, Xinyao Yi
Summary: The innovative method proposed for microRNA detection enhances sensitivity using duplex-specific nuclease and streptavidin-modified gold nanoparticles. The hybridization-enzymolysis recycling reactions in solution allow for simple operation, fast response, high sensitivity, and excellent specificity, making it a viable option for novel sensing platforms.
Article
Engineering, Electrical & Electronic
Santosh Kumar, Zhu Guo, Ragini Singh, Qinglin Wang, Bingyuan Zhang, Shuang Cheng, Feng-Zhen Liu, Carlos Marques, Brajesh Kumar Kaushik, Rajan Jha
Summary: This study introduces a fiber-optic localized surface plasmon resonance (LSPR) based biosensor for sensitive detection of Shigella bacterial species. By controlled etching and coating with nanomaterials, the sensor achieves increased refractive index sensitivity and excitation of localized plasmons. Results show successful and efficient detection of Shigella with high sensitivity, making it potentially valuable in the future as an alternative to existing commercial methods.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hirotaka Uzawa, Takehiro Nagatsuka, Yasuo Seto, Yoshihiro Nishida, Masato Saito, Eiichi Tamiya
Summary: Glycolipid chips with a double layer of Au nanoparticles were developed for detecting biological toxins. The chips consisted of an under and an upper layer of Au nanoparticles on glass plates, linked with 1,8-octanedithiol using a self-assembled monolayer technique. A tris-sialo glycosphingolipid, ganglioside GT1b, was immobilized on the upper layer and botulinum toxin was detected using localized surface plasmon resonance (LSPR). The GT1bCer-coated chip with a double layer of Au nanoparticles showed enhanced LSPR detection of the toxin compared to single monolayers. The combination of 60 and 40 nm Au nanoparticles in the under and upper layer, respectively, achieved the best results for toxin detection below 5 ng/mL using a portable LSPR device.
Article
Engineering, Electrical & Electronic
Yumei Zhang, Liyun Ding, Jue Zhao, Xingdong Jiang, Fei Ma
Summary: Acetylcholine is an important neurotransmitter that can be detected using a simple electrostatic interaction to create an optical fiber biosensor. The biosensor has high sensitivity and a linear range, making it more convenient, simple, and cost-effective compared to traditional detection methods.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Zhoutao Sun, Chen Kang, Xiaohui Fang, Hongmei Liu, Jinxin Guo, Xinping Zhang
Summary: Research demonstrates high sensitivity detection of crystal violet in aqueous solution using a capillary-based static liquid phase SERS method without the need for pre-functionalization, achieving an enhancement factor as high as 10^8 and a detection limit of 10^-9 M.
NANOSCALE ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Nur Afifah Ahmad Nazri, Nur Hidayah Azeman, Mohd Hafiz Abu Bakar, Nadhratun Naiim Mobarak, Tg Hasnan Tg Abd Aziz, Ahmad Rifqi Md Zain, Norhana Arsad, Yunhan Luo, Ahmad Ashrif A. Bakar
Summary: An optical sensor-based localized surface plasmon resonance (LSPR) sensor was demonstrated for sensitive and selective chlorophyll detection through the integration of amino-functionalized carbon quantum dots (NCQD) and triangle silver nanoparticles (AgNPs). The sensor showed high sensitivity and detection limits, with no interference from other ionic species.
Article
Engineering, Electrical & Electronic
Devi Taufiq Nurrohman, Nan-Fu Chiu
Summary: Gold nanoparticles (AuNPs) have unique properties and are widely used. This study investigates the signal characteristics of AuNPs-based localized surface plasmon resonance (LSPR) immunosensor through theoretical and experimental approaches. Simulation results show that the particle distance and the presence of dielectric material greatly affect the extinction and scattering spectra, while experimentally, the detection range of the sensor is limited by the enhanced electric field and the occurrence of the Hook effect at higher concentrations.
IEEE SENSORS JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Chien-Hsiu Li, Ming-Hsien Chan, Yu-Chan Chang, Michael Hsiao
Summary: Gold nanotechnology has revolutionized molecular biology applications, particularly in diagnosing and treating at the molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has enhanced molecule detection and increased the possibility of analyzing atoms. Gold nanoparticles offer superior analytical properties that can be utilized for drug screening, gene sequencing, and detecting physiological functions.
Review
Chemistry, Analytical
Fahad Usman, John Ojur Dennis, A. I. Aljameel, M. K. M. Ali, O. Aldaghri, K. H. Ibnaouf, Zakariyya Uba Zango, Mahnoush Beygisangchin, Ahmed Alsadig, Fabrice Meriaudeau
Summary: Lung cancer is the most common and deadliest cancer globally, and early diagnosis is crucial for survival. Optical biosensors, particularly plasmonic sensors, offer a promising solution for detecting lung cancer biomarkers, providing high sensitivity, freedom from electrical hazards, and multiplexing capability.
Article
Pharmacology & Pharmacy
Rayssa Helena Arruda Pereira, Wanderson Juvencio Keijok, Adilson Ribeiro Prado, Jairo Pinto de Oliveira, Marco Cesar Cunegundes Guimara
Summary: This study demonstrates a rapid and sensitive method for detecting trace levels of ochratoxin A in food by combining gold nanoparticles with anti-ochratoxin A IgG. The sensitivity of this nanosensor is very high, with a lower detection limit of about 0.001 pg mL-1, reducing detection limits by roughly 10 times compared to other methods.
Article
Chemistry, Physical
Shaopeng Chang, Lixuan Liu, Congpu Mu, Fusheng Wen, Jianyong Xiang, Kun Zhai, Bochong Wang, Leiming Wu, Anmin Nie, Yu Shu, Tianyu Xue, Zhongyuan Liu
Summary: We report a high-sensitivity surface plasmon resonance (SPR) RNA sensor using metallic 2D GeP5 nanosheets as the sensing material. Detection of SARS-CoV-2 RNA sequences was achieved with high sensitivity and excellent selectivity. This work demonstrates the immense potential of GeP5-based SPR sensors for advanced biosensing applications and paves the way for utilizing GeP5 nanosheets in novel sensor devices.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Agricultural Engineering
Aye Aye Myint, Patrick Hariyanto, Muhammad Irshad, Cao Ruqian, Sabrinna Wulandari, Min Eui Hong, Sang Jun Sim, Jaehoon Kim
Summary: A novel integrated extraction technique was demonstrated to achieve high recovery of natural astaxanthin from wet encysted Haematococcus pluvialis without the need for a pre-drying step. By researching the most suitable green solvent and optimizing the extraction conditions, a high recovery of astaxanthin and extraction yield were achieved. The biphasic solvent system played a key role in this process.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Ranjna Sirohi, Ashutosh Kumar Pandey, Panneerselvam Ranganathan, Shikhangi Singh, Aswathy Udayan, Mukesh Kumar Awasthi, Anh Tuan Hoang, Chaitanya Reddy Chilakamarry, Sang Hyoun Kim, Sang Jun Sim
Summary: There has been increasing attention on the use of photobioreactors for microalgae cultivation. Understanding the state-of-the-art designs, advantages, and limitations is crucial for classifying and applying different photobioreactors effectively.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Aswathy Udayan, Ranjna Sirohi, Nidhin Sreekumar, Byoung-In Sang, Sang Jun Sim
Summary: Microalgae are unicellular photosynthetic organisms that can produce high-value metabolites, making them potential candidates for nutraceutical and industrial products. Research on cost-effective cultivation systems and harvesting methods is crucial for increasing biomass yield and reducing downstream processing costs to fully utilize the potential of microalgae in industry.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Ayon Tarafdar, Ranjna Sirohi, Palanisamy Athiyaman Balakumaran, R. Reshmy, Aravind Madhavan, Raveendran Sindhu, Parameswaran Binod, Yogesh Kumar, Deepak Kumar, Sang Jun Sim
Summary: Bisphenol A (BPA) is a toxic endocrine disrupting chemical with immunosuppressive activity, disrupting the endogenous hormones and requiring proper detection and remediation methods. More research is needed on integrated remediation technologies and the impact of food processing operations on BPA.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Plant Sciences
Sabeela Beevi Ummalyma, Ranjna Sirohi, Aswathy Udayan, Pooja Yadav, Abhay Raj, Sang Jun Sim, Ashok Pandey
Summary: Microalgae are cell factories that can be used to produce renewable energy, food, feed, pharmaceuticals, and nutraceuticals. The challenges of mass cultivation and biomass recovery limit the industrial application of microalgae. Cultivating microalgae in wastewater has been encouraged for sustainable bioeconomy. Wastewater from the food industry provides a less-toxic growth medium for low-cost microalgal biomass production. Microalgae are potential biocatalysts for wastewater bioremediation and offer a universal alternative for resource recovery from wastewater.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Agricultural Engineering
Jaemin Joun, Ranjna Sirohi, Sang Jun Sim
Summary: This study compared the culture method using sodium acetate and glucose as organic carbon sources with autotrophy in Haematococcus pluvialis. In the 12-day culture, mixotrophy with sodium acetate and glucose increased biomass by 40.4% and 77.1% respectively compared to autotrophy, but the mechanisms were different. Mixotrophy with glucose increased biomass by directly supplying substrate and ATP to the TCA cycle while inhibiting photosynthesis. Gene expressions related to glycolysis and carbon fixation pathway were confirmed in autotrophy and mixotrophy with glucose and acetate. The proposed metabolism in mixotrophy with acetate and glucose was analyzed through autotrophic and heterotrophic metabolism.
BIORESOURCE TECHNOLOGY
(2023)
Correction
Agricultural Engineering
Ayon Tarafdar, Ranjna Sirohi, Vivek Kumar Gaur, Sunil Kumar, Poonam Sharma, Sunita Varjani, Hari Om Pandey, Raveendran Sindhu, Aravind Madhavan, Reshmy Rajasekharan, Sang Jun Sim
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Young Joon Sung, Byung Sun Yu, Ha Eun Yang, Dong Hoon Kim, Ju Yeon Lee, Sang Jun Sim
Summary: This study provides an in-depth analysis of microalgae-based biohydrogen production, focusing on large-scale cultivation systems and efficient downstream processes. Strategies to reduce carbon emissions and improve productivity simultaneously are discussed by considering each production process. This research has significant implications for achieving environmental and economic sustainability.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Jeong Seop Lee, Young Joon Sung, Dong Hun Kim, Ju Yeon Lee, Sang Jun Sim
Summary: A hybrid system for fabricating an easy-to-scale-up photosynthetic bioreactor (PBR) was developed, and the design of the baffle and the conditions of the gas sparger were optimized to improve biomass productivity. The newly developed PBR showed improved PHB production feasibility.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Ha Eun Yang, Byung Sun Yu, Sang Jun Sim
Summary: The aim of this study was to increase the productivity of biomass with high astaxanthin content by developing a mutant strain of Haematococcus pluvialis with strong environmental tolerance. The mutant M5 strain, selected through gamma irradiation, showed improved biomass and astaxanthin production under high-salinity and high-light intensity conditions. M5 demonstrated an increase in biomass and astaxanthin productivity by 86.70% and 66.15% respectively, compared to untreated cells.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
So Young Lee, Jeong Seop Lee, Sang Jun Sim
Summary: Wastewater treatment plants emit a large amount of greenhouse gases, but mixotrophic microalgae cultivation using wastewater has been proposed as a solution for CO2 mitigation and wastewater treatment. This study used waste soy sauce as a medium for Chlorella sorokiniana UTEX 2714 cultivation and found that using a 20% (v/v) 10-fold diluted soy sauce medium increased biomass and fatty acid methyl ester (FAME) productivity by 1.93 and 1.76 times, respectively. High biomass productivity was achieved under high-intensity light with a high soy sauce content, although it inhibited cell growth in photoautotrophic environments. Furthermore, the combination of industrial flue gas treatment with wastewater led to a 42% improvement in biomass production. These results suggest that mixotrophic microalgal cultivation has the potential to address climate change and water pollution while producing valuable products and contributing to a sustainable society.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biophysics
Sojin Song, Jong Uk Lee, Myeong Jin Jeon, Soohyun Kim, Chan-Nyoung Lee, Sang Jun Sim
Summary: Alzheimer's disease is a neurodegenerative condition that is challenging to diagnose early due to the lack of robust biomarkers and limited access to neuroimaging and cerebrospinal fluid tests. Exosomes in body fluids are being explored as diagnostic biomarkers that reflect neuropathological features. A programmable curved plasmonic nanoarchitecture-based biosensor has been developed to accurately diagnose Alzheimer's disease.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Plant Sciences
Jaemin Joun, Ranjna Sirohi, Byung Sun Yu, Ha Eun Yang, Sang Jun Sim
Summary: Microalgae cultivation using wastewater is an effective method for wastewater treatment and carbon footprint reduction. Wastewater provides essential nutrients for microalgae growth, while greenhouse gas emissions can be mitigated through Carbon Capture, Utilization, and Storage technology. This review comprehensively discusses the application of microalgae in capturing carbon and treating wastewater, highlighting the potential for improving productivity, reducing costs, and promoting environmental sustainability.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Ranjna Sirohi, V. Vivekanand, Ashutosh Kumar Pandey, Ayon Tarafdar, Mukesh Kumar Awasthi, Amita Shakya, Sang Hyoun Kim, Sang Jun Sim, Hoang A. Tuan, Ashok Pandey
Summary: The transition from fossil fuels to non-conventional sources is necessary for addressing the global energy crisis and environmental issues. Organic waste from various industries can be utilized to produce gaseous fuels through different biochemical strategies. However, current biogas and biohydrogen production practices are inefficient and require interventions to improve biofuel yield. Adding biochar has been shown to enhance gaseous fuel production by adsorbing inhibitors and activating gas-producing microorganisms. This review explores the effect of biochar on gaseous biofuel production and highlights the need for a circular bioeconomy approach.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Chemistry, Multidisciplinary
Sang Ki Kim, Jong Uk Lee, Myeong Jin Jeon, Soo-Kyung Kim, Sang-Hyun Hwang, Min Eui Hong, Sang Jun Sim
Summary: A colorimetric-fluorescent dual-mode lateral flow immunoassay sensor incorporating metal-enhanced fluorescence (MEF) was developed for the rapid and sensitive serological detection of SARS-CoV-2 IgG.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
