Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 618-624Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.003
Keywords
Fluorescent biosensors; DNA-templated silver nanoclusters; Fluorescence signal enhancement; Homo-adenine binding molecules; Coralyne
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Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning (MSIP) [2012M3A2A1051683]
- Center for BioNano Health-Guard - MSIP of Korea as Global Frontier Project [H-GUARD_2013M3A6B2078964]
- Industrial Source Technology Development Program of the Ministry of Knowledge Economy (MKE) [2010-10038662]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10038662] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2013M3A6B2078964, 2012M3A2A1051682] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A novel, label-free, turn-on fluorescence strategy to detect molecules that bind to adenine-rich DNA sequences has been developed. The probe employs DNA-templated silver nanoclusters (DNA-AgNCs) as the key detection component. The new strategy relies on the formation of non-Watson-Crick homo-adenine DNA duplex, triggered by strong interactions with homo-adenine binding molecules, which brings a guanine-rich sequence in one strand close to DNA-AgNCs located on the opposite strand. This phenomenon transforms weakly fluorescent AgNCs into highly emissive species that display bright red fluorescence. Finally, we have shown that the new fluorescence turn-on strategy can be employed to detect coralyne, the most representative homo-adenine binding molecule that triggers formation of a non-Watson-Crick homo-adenine DNA duplex. (C) 2014 Elsevier B.V. All rights reserved.
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