Journal
PLANT AND SOIL
Volume 448, Issue 1-2, Pages 479-494Publisher
SPRINGER
DOI: 10.1007/s11104-020-04448-w
Keywords
Metacaspase; AhMC1; Aluminum toxicity; PCD; Peanut root tips
Categories
Funding
- National Natural Science Foundation of China [31776190, 31560346, 31660350, 31701356, 31860334]
- Guangxi Natural Science Foundation of China [2016GXNSFBA380223]
- Training Program for 1000 Young and Middle-aged Backbone Teachers of Guangxi Higher Education Institution in 2019
Ask authors/readers for more resources
Aims Metacaspases are cysteine-dependent proteases, which play essential roles in programmed cell death (PCD), and caspase-3-like protease is the crucial executioner. However, its response mechanism to aluminum (Al)-induced PCD is still elusive. Methods Here, the type I metacaspase gene in peanut (Arachis hypoganea L.), AhMC1, was cloned from the Al-sensitive cultivar ZH2. Physiological and biochemical methods, as well as gene expression analyses, were employed to explore its function in Al-induced PCD in peanut root tips. Results AhMC1 had a 1068-bp open reading frame, encoding a peptide of 355 amino acids, and the purified protein exhibited a high caspase-3-like protease activity. Its expression levels in different tissues of peanut varieties ZH2 and 99-1507 (Al-tolerant) varied under Al-stress conditions. The subcellular localization indicated that AhMC1 was transferred from mitochondria into the cytoplasm. Furthermore, overexpressing AhMC1 reduced the resistance to Al stress. Sense transgenic plants showed a low relative root growth rate, and reduced superoxide dismutase, peroxidase, and catalase activities, compared with wild-type and antisense transgenic plants under Al-stress conditions, but had a high root-cell death rate, and increased Al and maleic dialdehyde contents. Conclusions The data suggest that metacaspase AhMC1 is a positive factor in Al-induced PCD in peanut root tips.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available