Article
Medicine, Research & Experimental
G. Kulikova, M. M. Ziganshina, A. Shchegolev, G. T. Sukhikh
Summary: The study found that the expression pattern of fucosylated glycans and the morphometric characteristics of the placental villi vary in patients with different severities of preeclampsia (PE), potentially affecting the function of the placental barrier.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Marina M. Ziganshina, Galina V. Kulikova, Kamilla T. Muminova, Alexander I. Shchegolev, Ekaterina L. Yarotskaya, Zulfiya S. Khodzhaeva, Gennady T. Sukhikh
Summary: This study analyzed the effect of antihypertensive therapy on the expression of fucosylated glycans in fetal capillaries and their correlation with maternal hemodynamics and other factors in patients with preeclampsia. The results showed that antihypertensive therapy affected the expression of fucosylated glycans and their correlation with maternal hemodynamics and other factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Marina M. Ziganshina, Nataliya V. Dolgushina, Galina V. Kulikova, Nafisa M. Fayzullina, Ekaterina L. Yarotskaya, Nailia R. Khasbiullina, Nigora F. Abdurakhmanova, Aleksandra V. Asaturova, Alexander I. Shchegolev, Alina A. Dovgan, Gennady T. Sukhikh
Summary: This study investigated the composition of the glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women. Compared to normal endometrium, thin endometrium showed significantly less expression of MECA-79 glycans, which are crucial for implantation. Unique glycans containing LacNAc unit and binding to MECA-79 MAbs were identified as potential markers of endometrium receptivity in thin endometrium.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
Salvatore Desantis, Giovanni Michele Lacalandra, Miguel Batista, Olga Amann, Dario Antonelli, Davide Monaco
Summary: The exposure of epididymal spermatozoa to seminal plasma affects the glycocalyx composition of dromedary camel ES, potentially enhancing assisted reproductive techniques. More comprehensive studies are needed to evaluate the fertilization capacity of seminal plasma-treated ES for their application in dromedary camel assisted reproduction technology.
Article
Biochemistry & Molecular Biology
Dayuan Shi, Xinyao Zhou, Luyao Cai, Xing Wei, Luye Zhang, Qianqian Sun, Fenhe Zhou, Luming Sun
Summary: Changes in placental DNA methylation patterns in sFGR may have functional implications for differentially methylated genes and regulatory regions. The study validates the presence of abnormally methylated CYP11A1 gene in the placenta of sFGR.
Article
Pharmacology & Pharmacy
Lewis J. Renshall, Frances Beards, Angelos Evangelinos, Susan L. Greenwood, Paul Brownbill, Adam Stevens, Colin P. Sibley, John D. Aplin, Edward D. Johnstone, Tambet Teesalu, Lynda K. Harris
Summary: This study demonstrates the potential of using EGF-loaded, peptide-decorated liposomes to improve placental function. GPS-decorated liposomes containing EGF significantly increased amino acid transporter activity in healthy placental tissues, but were ineffective in those from pregnancies with FGR. Targeted liposomes represent a safe and useful tool for developing new therapies for placental dysfunction.
Article
Environmental Sciences
Guangquan Chen, Shiyi Xiong, Qiao Jing, Cornelis A. M. van Gestel, Nico M. van Straalen, Dick Roelofs, Luming Sun, Hao Qiu
Summary: This study investigated the effects of polystyrene nanoplastics (PS-NPs) on fetal development and cholesterol metabolism in mice. The exposure to PS-NPs led to a decline in fetal weights and abnormal cell morphologies in the placenta and fetus. Transcriptomic and metabolomic analyses revealed disturbances in cholesterol metabolism and other pathways, offering new insights into the mechanisms underlying the detrimental effects of PS-NPs on placenta and fetus.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Hua-Long Zhu, Xue-Ting Shi, Xiao-Feng Xu, Yong-Wei Xiong, Song-Jia Yi, Guo-Xiang Zhou, Wei-Bo Liu, Miao-Miao Huang, Lan Gao, Cheng Zhang, Ling-Li Zhao, De-Xiang Xu, Hua Wang
Summary: The study found that environmental cadmium exposure can lead to fetal growth restriction by activating PERK-regulated placental mitophagy and inhibiting P4 synthesis. Additionally, placental mitophagy is associated with reduced progesterone levels and all-cause fetal growth restriction.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Environmental Sciences
Hao Zhang, Yi Zheng, Xiaoyun Liu, Xia Zha, Mabrouk Elsabagh, Yi Ma, Honghua Jiang, Hongrong Wang, Mengzhi Wang
Summary: This study investigated the potential relationship between oxidative stress, autophagy, and apoptosis in pregnant ewes exposed to BPA. The results showed that BPA exposure led to placental insufficiency, fetal growth restriction, autophagy, endoplasmic reticulum stress, mitochondrial dysfunction, oxidative stress, and apoptosis. Additionally, the study revealed that autophagy could alleviate BPA-induced apoptosis, mitochondrial dysfunction, and oxidative stress.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Immunology
Huan Yang, Jie He, Shuai Huang, Hongbing Yang, Qingjie Yi, Yuelan Tao, Miaomiao Chen, Xuemei Zhang, Hongbo Qi
Summary: The study identified a large number of eccDNAs/ecDNAs in FGR, highlighting their potential association with factors such as gestational age. These molecules were found to be mainly enriched in immune-related functions and pathways, overlapping with non-coding RNAs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Developmental Biology
A. Arias, J. A. Schander, M. Bariani, F. Correa, A. P. Dominguez Rubio, M. Cella, C. B. Cymeryng, M. L. Wolfson, A. M. Franchi, J. Aisemberg
Summary: Maternal dexamethasone administration during late gestation results in fetal and placental growth restriction, potentially due to altered placental angiogenesis.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
R. Aughwane, N. Mufti, D. Flouri, K. Maksym, R. Spencer, M. Sokolska, G. Kendall, D. Atkinson, A. Bainbridge, J. Deprest, T. Vercauteren, S. Ourselin, A. L. David, A. Melbourne
Summary: The study found that MRI technique can reveal differences in feto-placental oxygen saturation between normal and early-onset fetal growth restriction (FGR) pregnancies, and this difference is associated with disease severity.
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2021)
Article
Reproductive Biology
Jessica F. Hebert, Jess A. Millar, Rahul Raghavan, Amie Romney, Jason E. Podrabsky, Monique Y. Rennie, Allison M. Felker, Perrie O'Tierney-Ginn, Mayu Morita, Elizabeth A. DuPriest, Terry K. Morgan
Summary: Research in a mouse model indicates that maternal AGT genetic variants can lead to reduced uteroplacental angiogenesis, particularly in male fetuses. This suggests that male fetal sex exacerbates the pathologic impact of maternal genotype on growth restriction.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Hua-Long Zhu, Xue-Ting Shi, Xiao-Feng Xu, Guo-Xiang Zhou, Yong-Wei Xiong, Song-Jia Yi, Wei-Bo Liu, Li-Min Dai, Xue-Lin Cao, De-Xiang Xu, Hua Wang
Summary: Gestational exposure to environmental stress can lead to fetal growth restriction (FGR) with reduced levels of melatonin (MT) in the placenta. MT offers protective effects against FGR by inhibiting BNIP3-dependent mitophagy, potentially serving as a therapeutic agent against environmental stress-induced developmental toxicity.
Article
Biochemistry & Molecular Biology
Natasha de Alwis, Sally Beard, Natalie K. Binder, Natasha Pritchard, Tu'uhevaha J. Kaitu'u-Lino, Susan P. Walker, Owen Stock, Katie Groom, Scott Petersen, Amanda Henry, Joanne M. Said, Sean Seeho, Stefan C. Kane, Stephen Tong, Lisa Hui, Natalie J. Hannan
Summary: Elevated levels of OLAH in the placenta may be associated with placental dysfunction in pregnancies complicated by preterm preeclampsia and fetal growth restriction. Further research is required to understand the role of OLAH in the placenta, and whether these changes are a maternal adaptation or consequence of disease.
Article
Hematology
Radhika Nagappan, Willy A. Flegel, Kshitij Srivastava, Eleanor C. Williams, Ivan Ryzhov, Alexander Tuzikov, Oxana Galanina, Nadezhda Shilova, Gennady Sukhikh, Holly Perry, Nicolai V. Bovin, Stephen M. Henry
Summary: This study evaluated the feasibility of using SARS-CoV-2 peptide Kode Technology-modified red cells to develop an assay for detecting SARS-CoV-2 antibodies. The results showed that C19-kodecytes have high specificity and sensitivity on routine serologic platforms, making them suitable for serological testing related to COVID-19.
Article
Biochemical Research Methods
Ivan M. Ryzhov, Alexander B. Tuzikov, Alexey Nizovtsev, Ludmila K. Baidakova, Oxana E. Galanina, Nadezhda Shilova, Marina M. Ziganshina, Nataliya Dolgushina, Guldana R. Bayramova, Gennady T. Sukhikh, Eleanor C. Williams, Radhika Nagappan, Stephen M. Henry, Nicolai Bovin
Summary: In the near future, the number of required COVID-19 antibody tests is expected to increase to hundreds of millions, utilizing a variety of analytical methods. This study compared three method variations for detecting specific immunoglobulins against peptides of the SARS-CoV-2 spike protein. The results showed that the function-spacer-lipid constructs performed better, particularly in erythrocyte-based serologic tests.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Federico M. Ruiz, Francisco J. Medrano, Anna-Kristin Ludwig, Herbert Kaltner, Nadezhda V. Shilova, Nicolai V. Bovin, Hans-Joachim Gabius, Antonio Romero
Summary: This study presents the first crystallographic information on the N-terminal extension of rat galectin-5, revealing the structured organization around the central Trp77 of the carbohydrate recognition domain. The ligand-bound structure demonstrates a shift in the amino acid alignment, potentially acting as a molecular switch for new contacts in this region.
Article
Immunology
Marina M. Ziganshina, Nadezhda Shilova, Nailia R. Khasbiullina, Anastasia Terentyeva, Elena L. Dolgopolova, Alexey Yu Nokel, Ekaterina L. Yarotskaya, Roman G. Shmakov, Nicolai Bovin, Gennady T. Sukhikh
Summary: A possible mechanism of immune tolerance in pregnancy is the production of blocking antibodies in the placenta, which protect the fetus from the mother's immune system. The specificity and nature of these antibodies are still unknown. However, the study found that the repertoire of placental antibodies is less variable and qualitatively different from those in the peripheral blood, and this repertoire is narrower in women with pre-eclampsia compared to healthy pregnancy. Some antibodies are found exclusively in the placenta, while others are only found in the placenta of healthy women.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Nikiforova, Victoria V. Golovchenko, Polina Mikshina, Olga A. Patova, Tatyana A. Gorshkova, Nikolai Bovin, Nadezhda Shilova
Summary: This study deciphers the carbohydrate specificity of plant proteins using glycoarrays and discovers the ability of ricin to bind polysaccharides, expanding the knowledge of its specificity. Additionally, it finds a wide variety of antibodies to plant polysaccharides in human peripheral blood.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Biochemistry & Molecular Biology
Nadezhda Shilova, Nicolai Bovin, Diana Maltseva, Svetlana Polyakova, Marina Sablina, Hideaki Niwa, Galina Zakharova, Maria Raygorodskaya, Lyuba Bufeeva, Yury Belyi, Dmitry Hushpulian, Alexander Tonevitsky
Summary: Viscumin, a lectin used in anti-cancer therapy, has been found to have the ability to bind glycans with 6-O-SuGal moiety and some aGal-terminated glycans. However, its interaction with parent neutral f3-galactosides is weak. Further studies reveal that viscumin's two glycan-binding sites have different specificities, with one preferring charged sulfated and sialylated moieties.
Article
Biochemistry & Molecular Biology
E. Slivka, A. B. Tuzikov, S. Khaidukov, V. A. Komarova, S. M. Henry, N. Bovin, E. M. Rapoport
Summary: This study compared the properties of three synthetic analogs and found that they have similar localization patterns and can be interchanged when studying the incorporation/release processes. However, there are some differences in the insertion process and release kinetics.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marina M. Ziganshina, Kamilla T. Muminova, Nailia R. Khasbiullina, Zulfiya S. Khodzhaeva, Ekaterina L. Yarotskaya, Gennady T. Sukhikh
Summary: This paper assesses the changes in blood vessels during preeclampsia and describes the vascular patterns. The study found that in early-onset preeclampsia, there were changes in all structural components of endothelial glycocalyx, as well as severe disorders in central hemodynamics, arterial stiffness, and myocardial function. In late-onset preeclampsia, there were changes in hyaluronic acid levels, central hemodynamics, and myocardial function, which may indicate potential cardiovascular disorders.
Article
Parasitology
Marinela Contreras, Rita Vaz-Rodrigues, Lorena Mazuecos, Margarita Villar, Sara Artigas-Jeronimo, Almudena Gonzalez-Garcia, Nadezhda V. Shilova, Nicolai V. Bovin, Sandra Diaz-Sanchez, Elisa Ferreras-Colino, Ivan Pacheco, Jindrich Chmelar, Petr Kopacek, Alejandro Cabezas-Cruz, Christian Gortazar, Jose de la Fuente
Summary: This study investigates the role of tick salivary components and proteins with and without alpha-Gal modifications in modulating the immune response against the carbohydrate. The results show that exposure to tick saliva leads to allergic reactions, abnormal behavior, and mortality in zebrafish. The antibody titers against alpha-Gal and tick salivary gland proteins are significantly higher in the saliva protein fractions and deglycosylated saliva group.
PARASITES & VECTORS
(2023)
Review
Virology
Marina M. Ziganshina, Nadezhda V. Shilova, Eugenia O. Khalturina, Natalya V. Dolgushina, Sergey Borisevich, Ekaterina L. Yarotskaya, Nicolai V. Bovin, Gennady T. Sukhikh
Summary: Antibody-dependent enhancement (ADE) is a phenomenon where virus-specific antibodies paradoxically cause enhanced viral replication and/or excessive immune responses, leading to infection exacerbation, tissue damage, and multiple organ failure. The evidence for ADE in COVID-19 is insufficient and prediction and confirmation of ADE are challenging due to the lack of specific laboratory markers. The role of anti-glycan antibodies (AGA) in ADE in viral infections, including COVID-19, has not been studied extensively.
Article
Biochemistry & Molecular Biology
P. S. Obukhova, M. M. Ziganshina, N. Shilova, A. A. Chinarev, G. Pazynina, A. Y. Nokel, A. Terenteva, N. R. Khasbiullina, G. T. Sukhikh, A. A. Ragimov, E. L. Salimov, V. Butvilovskaya, S. M. Polyakova, J. Saha, N. Bovin
Summary: Previous studies have shown that healthy individuals lack natural antibodies against sialylated glycoproteins, but have moderate levels of antibodies against unnatural oligosaccharides. This study focused on investigating antibodies against beta Neu5Ac in detail, and discovered the presence of these antibodies in pregnant women.
Review
Nanoscience & Nanotechnology
A. Yu Nokel, N. Bovin, N. Shilova
Summary: The review discusses the main methods for modifying plastic as a functional material for manufacturing biochips, including physical, chemical, and physical-chemical methods for introducing functional groups and achieving effective immobilization of biomolecules. The advantages and disadvantages of existing approaches are discussed in terms of their applicability to various tasks.
NANOBIOTECHNOLOGY REPORTS
(2022)
