Article
Biophysics
Melanie MacGregor, Hanieh Safizadeh Shirazi, Kit Man Chan, Kola Ostrikov, Kym McNicholas, Alex Jay, Michael Chong, Alexander H. Staudacher, Thomas D. Michl, Aigerim Zhalgasbaikyzy, Michael P. Brown, Moein Navvab Kashani, Adam Di Fiore, Alex Grochowski, Stephen Robb, Simon Belcher, Jordan Li, Jonathan M. Gleadle, Krasimir Vasilev
Summary: The non-invasive diagnostic platform presented in this study combines microfluidic technology with PDD principles and immunocapture technology to detect shed bladder cancer cells in patient urine, achieving highly specific capture rates. In a pilot study, it successfully differentiated bladder cancer patients from non-cancer controls, showing potential for post-treatment surveillance and cancer detection in suspected bladder cancer patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Cell Biology
Jinshan Xu, Shuxiong Zeng, Jun Li, Li Gao, Wenjun Le, Xin Huang, Guandan Wang, Bingdi Chen, Zhensheng Zhang, Chuanliang Xu
Summary: The study developed a novel UCT method called Nano-cytology by mixing urine with mNPs to improve the sensitivity of bladder cancer detection. The Nano-cytology assay showed significantly higher sensitivity compared to the UCT assay, and the combination of the two approaches further increased sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Antonio Domenech-Carbo, Jose Luis Pontones, Clara Domenech-Casasus, David Ramos
Summary: The theoretical model on chemical signaling for diagnosis combines signals for marker and inert/interfering metabolites, resulting in asymptotic relationships between signal intensities. Voltammetric features associated with oxidation of urine metabolites and Zn (II) reduction were well-matched with the model, leading to a non-invasive electrochemical detection of bladder cancer. Different diagnostic criteria displayed high sensitivity, specificity, and positive/negative predictive values.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Oncology
Hideo Fukuhara, Takahiro Nohara, Koshiro Nishimoto, Yutaka Hatakeyama, Yuki Hyodo, Yoshiyasu Okuhara, Masafumi Oyama, Atsushi Mizokami, Keiji Inoue, Hideyasu Matsuyama
Summary: This study investigated risk factors for orally administered 5-aminolevulinic acid-induced hypotension in bladder cancer patients undergoing photodynamic diagnosis-assisted transurethral resection of bladder tumor. The results showed that a history of hypertension and general anesthesia were associated with increased risk of hypotension, while the use of calcium antagonists was associated with reduced risk of hypotension.
Article
Urology & Nephrology
Yair Lotan, Iftach Chaplin, Hamed Ahmadi, Xiaosong Meng, Sidney Roberts, Sanam Ladi-Seyedian, Aditya Bagrodia, Vitaly Margulis, Solomon Woldu, Siamak Daneshmand
Summary: The study evaluated the utility of blue-light flexible cystoscopy (BLFC) for surveillance of non-muscle-invasive bladder cancer (NMIBC). Results showed that incorporating BLFC in clinical practice has the potential advantages of detecting cancer in cases with normal WLC and detecting additional cancers in patients with positive WLC and BLFC, which can improve surveillance and performance of office-based biopsy. Further research is needed to determine cost-effectiveness and impact on recurrence rates.
Article
Chemistry, Analytical
Regina Belugina, Evgenii Karpushchenko, Aleksandr Sleptsov, Vladimir Protoshchak, Andrey Legin, Dmitry Kirsanov
Summary: This study explores the feasibility of using a simple electrochemical multisensor system to distinguish between urine samples from patients with confirmed bladder cancer and healthy volunteers. The best model achieved good accuracy and sensitivity with data from individuals of different ages and tumor grades, with even better performance observed in a limited age group. The proposed screening method shows promise for further research due to its simplicity.
Article
Biophysics
Mohammed Zniber, Parastoo Vahdatiyekta, Tan-Phat Huynh
Summary: Electronic tongues have been widely used in quality monitoring and disease diagnosis. However, their application in cancer diagnosis from urine samples is underestimated. This review highlights various analytical techniques and machine learning methods used in e-tongues for urine analysis, emphasizing their ability to distinguish between patients diagnosed with cancer and healthy controls.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Li Yan, Congcong Shen, Pengjun Jiang, Yunjin Bai, Runlian Qu, Wu Peng, Zeliang Wei, Ke Huang, Jing Zhou, Rundong Zhang, Piaopiao Chen
Summary: This study proposed a homogeneous enzyme-free and nucleic acid-based catalytic hairpin assembly and cascade recognition reactions strategy for the dual visualization and fluorescence signal detection of bladder cancer marker miRNA-126 in clinical urine. The sensitivity of miRNA analysis was significantly improved by integrating cascade amplification and material cation exchange reaction. The experimental results were consistent with other detection methods and the assay showed high specificity and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Oncology
Rikiya Taoka, Yuki Matsuoka, Mari Yamasaki, Naoya Kani, Tomoko Honda, Satoshi Harada, Yoichiro Tohi, Takuma Kato, Homare Okazoe, Hiroyuki Tsunemori, Nobufumi Ueda, Mikio Sugimoto
Summary: The study over 11 years showed that using 5-aminolevulinic acid-photodynamic diagnosis during transurethral resection can reduce residual bladder cancer and improve the cumulative intravesical recurrence-free survival in patients with non-muscle-invasive bladder cancer.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Gibum Lee, Wonseok Choi, Chulhong Kim, Sei Kwang Hahn, Jung Ho Lee
Summary: Researchers have developed a hyaluronate-BP-upconversion nanoparticle (HA-BP-UCNP) complex for non-invasive multimodal therapy of skin cancer using near-infrared light for photoacoustic imaging, photodynamic therapy, and photothermal therapy.
Review
Urology & Nephrology
Julia Oto, Alvaro Fernandez-Pardo, Marta Roca, Emma Plana, Fernando Cana, Raquel Herranz, Javier Perez-Ardavin, Cesar David Vera-Donoso, Manuel Martinez-Sarmiento, Pilar Medina
Summary: This study identified novel non-invasive diagnostic and staging biomarkers for bladder cancer through mass spectrometry-based metabolomics, which may improve the management of the disease.
WORLD JOURNAL OF UROLOGY
(2022)
Article
Urology & Nephrology
Keita Kobayashi, Hideyasu Matsuyama, Taketo Kawai, Atsushi Ikeda, Makito Miyake, Koshiro Nishimoto, Yuto Matsushita, Kazumasa Komura, Takashige Abe, Haruki Kume, Hiroyuki Nishiyama, Kiyohide Fujimoto, Masafumi Oyama, Hideaki Miyake, Keiji Inoue, Takahiko Mitsui, Mutsushi Kawakita, Chikara Ohyama, Atsushi Mizokami, Hajime Kuroiwa
Summary: The study evaluated the efficacy of photodynamic diagnosis-transurethral resection of bladder tumor for high-risk non-muscle invasive bladder cancer, showing a significantly lower residual tumor rate in the photodynamic diagnosis group compared to the white-light group.
INTERNATIONAL JOURNAL OF UROLOGY
(2022)
Article
Medicine, General & Internal
Makito Miyake, Yasushi Nakai, Tomomi Fujii, Takuya Owari, Shunta Hori, Yosuke Morizawa, Daisuke Gotoh, Satoshi Anai, Kazumasa Torimoto, Nobumichi Tanaka, Yoshihiko Hirao, Kiyohide Fujimoto
Summary: Clinical evidence suggests that PDD assistance reduces the risk of repeated bladder recurrence in NMIBC patients following TURBT, with no instances of multiple recurrence in the PDD-TURBT group within 1000 postoperative days. Compared to WL-TURBT, PDD assistance decreases 6.6 recurrences per 10,000 person-days, indicating its potential as a better prognostic factor for NMIBC patients.
Article
Multidisciplinary Sciences
Javier Monreal-Trigo, Miguel Alcaniz, M. Carmen Martinez-Bisbal, Alba Loras, Lluis Pascual, Jose Luis Ruiz-Cerda, Alberto Ferrer, Ramon Martinez-Manez
Summary: Bladder cancer is a significant cause of cancer-related deaths, and patients require surgery and lifelong surveillance. This study successfully used a voltammetric electronic tongue to analyze urine samples and differentiate between bladder cancer and non-bladder cancer. The results provide a promising approach for the development of non-invasive, low-cost, and user-friendly strategies for bladder cancer diagnosis and follow-up.
Article
Urology & Nephrology
Michele Morelli, Michael Baboudjian, Davide Vanacore, Bastien Gondran-Tellier, Robin McManus, Gilles Karsenty, Eric Lechevallier, Emanuele Montanari, Romain Boissier
Summary: The systematic use of PDD after BCG induction increased the detection rate of BCG-refractory tumors in high-risk NMIBC patients, leading to significant modifications in treatment approaches. PDD-C showed higher sensitivity and specificity in detecting BC compared to WL-C.
JOURNAL OF ENDOUROLOGY
(2021)
Article
Chemistry, Physical
Bidhari Pidhatika, Melanie Macgregor
Summary: A proof of concept study was conducted to develop dual-functional surfaces that can provide both biopassivity and bioactivity. A biopassive platform was created by grafting acetylene-decorated PMOXA brushes onto an Nb2O5 surface. Various azide-decorated compounds of different sizes and chemical structures were then reacted with the biopassive brush platform through a click reaction. The characterization results showed successful binding of benzyl-triazole fragments, specific and nonspecific interactions between mannose-azide molecules and the PMOXA-acetylene brush, and a successful reaction between AMP-azide and the PMOXA-acetylene brush platform. These results validated the original approach of generating dual-functional surfaces.
SURFACE REVIEW AND LETTERS
(2023)
Article
Nanoscience & Nanotechnology
Xuan Luo, Wenjin Xing, Iliana Delcheva, Fayed Abdullah Alrashaidi, Amir Heydari, Dennis Palms, Vi Khanh Truong, Krasimir Vasilev, Zhongfan Jia, Wei Zhang, Peng Su, Kasturi Vimalanathan, Aghil Igder, Gregory A. Weiss, Youhong Tang, Melanie MacGregor, Colin L. Raston
Summary: A shear-stress-mediated platform called portable vortex-fluidic device (P-VFD) has been developed to overcome the diffusion-limit and achieve fast reaction rates. The platform consists of a plasma oxazoline-coated polyvinyl chloride (POx-PVC) film with tough hydrogel covalently bound to its surface and a reactor tube for reactions. The hydrogel arrays on the film provide a strong matrix for entrapping biomolecules and tolerate shear stress, resulting in a >6-fold increase in reaction rate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Optics
Vuyelwa Ncapayi, Neethu Ninan, Thabang C. Lebepe, Sundararajan Parani, Aswathy Ravindran Girija, Richard Bright, Rodney Maluleke, Olanrewaju A. Aladesuyi, Tetsuya Kodama, Krasimir Vasilev, Oluwatobi S. Oluwafemi
Summary: In this study, NIR emitting colloidal Mn-doped CuInSe/ZnS QDs were synthesized via the hydrothermal method and showed good photostability. The QDs exhibited selective imaging of Gram-positive bacteria and prostate cancer cells, making them potential candidates for cancer-bacteria dual-imaging in biomedicine.
JOURNAL OF LUMINESCENCE
(2023)
Article
Physics, Applied
Alexandru Gheorghiu, Daisy Yang, Iliana Delcheva, Craig Priest, Melanie MacGregor
Summary: Plasma-polymerized polyoxazoline (POx) thin films can be used as a fast and solvent-free method for electrode functionalization. This study investigated the effects of current exposure, extended incubation, and repeated electrode rinses on the stability of polymethyloxazoline thin films. The films were found to become more diffusive after incubation and rinse steps, and changes in nanotopography were observed after exposure to current, suggesting a change in the film structure.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Chemistry, Physical
Pawel Mierczynski, Agnieszka Mierczynska-Vasilev, Krasimir Vasilev, Malgorzata I. Szynkowska-Jozwik
Summary: This study presents a mechanistic investigation of methane oxy-steam reforming and LNG (OSR-LNG) processes using Fourier transform infrared (FTIR) spectroscopy for the first time. FTIR measurements were conducted in the temperature range of 200-600 degrees Celsius to propose the reaction mechanisms of oxy-steam reforming of CH4 or LNG. It was discovered that the dissociation of methane into carbon-containing molecules and/or onto the catalyst surface is a crucial step in determining the reaction rate. These findings can be applied to the design of catalytic systems for natural gas reforming.
REACTION KINETICS MECHANISMS AND CATALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Yang Yu, Houyi Sun, Qunshan Lu, Junyuan Sun, Pengfei Zhang, Linran Zeng, Krasimir Vasilev, Yunpeng Zhao, Yu Chen, Peilai Liu
Summary: CaO2-loaded 2D titanium carbide nanosheets (CaO2-TiOx@Ti3C2, C-T@Ti3C2) with sonodynamic and chemodynamic features were developed. These nanosheets exhibited ideal antibacterial effects and promoted osteogenic transformation, which are crucial for the treatment of bacterial infections and wound healing.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Pawel Mierczynski, Agnieszka Mierczynska-Vasilev, Waldemar Maniukiewicz, Krasimir Vasilev, Malgorzata Szynkowska-Jozwik
Summary: In this study, multi-walled carbon nanotubes (MWCNTs) were prepared by chemical vapour deposition (CVD) using acetylene as a carbon source over an iron catalyst. The feasibility of MWCNTs supported monometallic and bimetallic catalysts for hydrogen production during steam reforming of methanol (SRM) and methanol decomposition (DM) was investigated. SEM and XRD methods were used to evaluate the structural characteristics of MWCNTs. TPR and XRD methods were used to analyze the physicochemical properties of the catalysts. The promotion effect of palladium on methanol conversion rate and H2 productivity in the case of the copper catalysts was demonstrated, which was attributed to the formation of Pd-Cu alloy compound.
Editorial Material
Chemistry, Multidisciplinary
Krasimir Vasilev
Article
Chemistry, Multidisciplinary
Evelyn Osehontue Uroro, Richard Bright, Jing Yang Quek, Krasimir Vasilev
Summary: A novel approach for drug administration using stimulus-responsive carriers is introduced in this study to minimize drug dosage and frequency, thereby reducing cytotoxicity levels. Nanocomposites loaded with rifampicin (RFP) were prepared using lipases-sensitive polycaprolactone (PCL) and showed responsive behavior and improved biocompatibility compared to the free drug.
Article
Engineering, Biomedical
Junyuan Sun, Fei Yang, Lianlei Wang, Haichao Yu, Zhijie Yang, Jingjing Wei, Krasimir Vasilev, Xuesong Zhang, Xinyu Liu, Yunpeng Zhao
Summary: Stem cell transplantation is a promising therapeutic method for intervertebral disc degeneration. However, oxidative stress in the degenerated region impairs the efficiency of the treatment. In this study, Coenzyme Q10 was encapsulated in lecithin micelles and successfully delivered to stem cells in vitro and in animal models, providing a potential therapeutic agent for intervertebral disc degeneration.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
E. O. Uroro, R. Bright, P. R. Lakshika Dabare, J. Y. Quek, N. Goswami, K. Vasilev
Summary: This study developed an enzyme-responsive antibacterial nanomaterial that selectively releases antibacterial agents in the presence of bacterial lipase. It showed minimal toxicity to human fibroblast cells, indicating potential applications in healthcare products and devices for infection prevention and treatment.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Tuyet Pham, Tien Thanh Nguyen, Ngoc Huu Nguyen, Andrew Hayles, Wenshao Li, Duy Quang Pham, Chung Kim Nguyen, Trung Nguyen, Jitraporn Vongsvivut, Neethu Ninan, Ylias Sabri, Wei Zhang, Krasimir Vasilev, Vi Khanh Truong
Summary: This study presents a novel sustainable technology, using argon atmospheric plasma jet, to transform microalgae into bioactive coatings with excellent antibacterial activity and biological compatibility. This technology has the potential to combat wound infections and advance wound care research.
Review
Pharmacology & Pharmacy
Yanping He, Krasimir Vasilev, Peter Zilm
Summary: Dental caries is a multifactorial biofilm disease caused by cariogenic bacteria that ferment carbohydrates to lactic acid, leading to a loss of minerals in teeth. Developments in pH-responsive delivery systems that release antibacterial agents in response to low pH have shown promise as a targeted therapy for dental caries.
Article
Materials Science, Multidisciplinary
Jesus Romo-Rico, Richard Bright, Smriti Murali Krishna, Krasimir Vasilev, Jonathan Golledge, Mohan V. Jacob
Summary: In this research, graphene (Gr) was deposited on a medical-grade cobalt-chromium (CoCr) alloy surface using Origanum vulgare as a precursor material. The biocompatibility and antibacterial properties of CoCr-Gr were investigated. The results suggest that CoCr-Gr could be a potential antibacterial coating material for implantable devices.
Article
Nanoscience & Nanotechnology
Panthihage Ruvini L. Dabare, Akash Bachhuka, Jing Yang Quek, Lluis F. F. Marsal, John Hayball, Krasimir Vasilev
Summary: It has been shown that macrophage polarization can be regulated by modifying the surface features of biomaterials, and this effect can be maintained over a prolonged period. In this study, differentiated THP-1 cells were used to control macrophage polarization on nano-rough surfaces for 7 days. The results demonstrate that regardless of surface roughness, the expression of pro-inflammatory cytokines decreases while the expression of anti-inflammatory cytokines increases, and the predominant cell type on the modified surfaces exhibits an M2 anti-inflammatory phenotype.