Article
Entomology
Xiaobin Zheng, Yanran Wan, Min Tao, Jiangjiang Yuan, Kun Zhang, Jing Wang, Youjun Zhang, Pei Liang, Qingjun Wu
Summary: The Obst protein plays an important role in the acquisition and transmission of Tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis, while the ApoD and Orai proteins do not have this effect.
Article
Agronomy
Min Tao, Yanran Wan, Xiaobin Zheng, Kanghua Qian, Austin Merchant, Baoyun Xu, Youjun Zhang, Xuguo Zhou, Qingjun Wu
Summary: This study investigated the genetic basis of the male-biased sex ratio in Frankliniella occidentalis, a vector of tomato spotted wilt orthotospovirus (TSWV), using proteomic analysis and RNAi technology.
PEST MANAGEMENT SCIENCE
(2022)
Article
Microbiology
Wanhong Zhang, Yubing Jiao, Chengying Ding, Lili Shen, Ying Li, Yanbi Yu, Kun Huang, Bin Li, Fenglong Wang, Jinguang Yang
Summary: A sensitive detection system based on CRISPR/Cas13a was developed for rapid and accurate diagnosis of tomato spotted wilt virus (TSWV), demonstrating high selectivity and improved sensitivity compared to traditional RT-PCR methods.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Hongwei Wang, Xiujuan Wu, Xiande Huang, Shujun Wei, Zhijun Lu, Jian Ye
Summary: This study provides evidence for the seed-borne transmission of Tomato spotted wilt orthotospovirus (TSWV) in pepper plants. The study shows that TSWV can be transmitted through seeds, with the endosperm being the main virus-containing seed organ. These findings are significant for researchers, certification agencies, the seed industry, and policy makers when evaluating the importance of TSWV in vegetable production worldwide.
Article
Biochemistry & Molecular Biology
Zhijun Zhang, Jiahui Zhang, Xiaowei Li, Jinming Zhang, Yunsheng Wang, Yaobin Lu
Summary: The transmission of insect-borne viruses involves intricate interactions between viruses, host plants, and vectors. Chemical compounds play a vital role in these interactions. This study demonstrates that a plant virus infection can alter the chemical composition of its host plant, making it more attractive to its vector and enhancing the vector's biological performance. These findings provide insights into the physiological mechanisms behind the virus's benefits to its vector.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chul-Young Kim, Yonggyun Kim
Summary: The nonstructural protein (NSs) of Tomato spotted wilt virus acts as a silencing suppressor in insects and can be used as a synergist for RNA pathogens to control insect pests.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Microbiology
Pin-Chu Lai, Mark R. Abney, Sudeep Bag, Albert K. Culbreath, Rajagopalbabu Srinivasan
Summary: Investigating population genetics of Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) isolates collected from peanut cultivars with varying levels of TSWV resistance revealed no evidence of resistance exerting selection pressure on TSWV. Despite differences in susceptibility to thrips among evaluated cultivars, no significant impact of resistance on TSWV was found.
Article
Agronomy
Tatsuya Kon, Noriko Watanabe, Hironori Ootake, Shin-ichi Fuji
Summary: Diseases associated with tomato spotted wilt orthotospovirus (TSWV) pose a serious threat to vegetable production worldwide. A new strain of TSWV, designated as TSWV-Fukushima:cucumber 2019 (TS-FC19), was identified as the causative agent of cucumber leaf necrosis disease in Fukushima Prefecture. This is the first detailed report on the biological characteristics of a cucumber-infecting TSWV.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Virology
Hongzheng Tao, Zhiqiang Jia, Xue Gao, Min Gui, Yongzhong Li, Yating Liu
Summary: MicroRNAs (miRNAs) that are regulated by TSWV infection in peppers were identified using high-throughput sequencing technology and their functions and target genes were analyzed. The study found 101 differentially expressed miRNAs in TSWV-infected peppers and several of these miRNAs were validated. The function of these miRNAs and their target genes were predicted through functional and pathway enrichment analysis, revealing their regulatory role in plant-pathogen interactions and plant hormone signal transduction. These findings provide insights into the mechanism of TSWV-host interactions.
Article
Microbiology
Yi-Ju Chen, Sudeep Pandey, Michael Catto, Soraya Leal-Bertioli, Mark R. Abney, Sudeep Bag, Mark Hopkins, Albert Culbreath, Rajagopalbabu Srinivasan
Summary: This study demonstrates the resistance of wild diploid Arachis species and peanut-compatible-induced allotetraploids against TSWV and thrips through thrips-mediated transmission assays and feeding assays. Among the evaluated genotypes, certain diploids and tetraploid hybrids exhibited reduced TSWV infection, virus accumulation, and thrips feeding damage.
Article
Agriculture, Multidisciplinary
Branka Petrovic, Ana Vucurovic, Katarina Zecevic, Goran Delibasic, Branka Krstic, Ivana Stankovic
Summary: The study identified resistance-breaking and resistance-inducing isolates of TSWV in Serbia, indicating independent evolutionary events. Phylogenetic analyses revealed gene flow between different regions, highlighting the importance of continued monitoring to prevent the establishment of resistant TSWV isolates.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Agronomy
Shohei Matsuura, Yushi Takehara, Tamito Sakurai
Summary: We evaluated the suppressive effect of prohydrojasmon (PDJ) on Frankliniella occidentalis and tomato spotted wilt virus (TSWV) in chrysanthemums under semi-commercial conditions. The overhead sprinkling of plants with PDJ significantly reduced feeding damage on leaves and the reproduction of larval offspring. PDJ also suppressed the occurrence of TSWV transmitted by viruliferous adult thrips, suggesting its potential in controlling orthotospovirus diseases.
Article
Virology
Naga Charan Konakalla, Sudeep Bag, Anushi Suwaneththiya Deraniyagala, Albert K. Culbreath, Hanu R. Pappu
Summary: The study demonstrated the effectiveness and feasibility of inducing RNA interference (RNAi) resistance against TSWV in tobacco plants using a non-transgenic strategy. By examining the mechanisms of action and transmission of double-stranded RNA molecules, the research showed the potential for managing TSWV in tobacco crops through topical application of dsRNAs.
Article
Fisheries
Chul-Young Kim, Shabbir Ahmed, David Stanley, Yonggyun Kim
Summary: Tomato spotted wilt virus (TSWV) is transmitted by specific thrips, including the western flower thrips, and induces immune responses in the insects. We found that TSWV infection triggers the release of a damage-associated molecular pattern called DSP1, leading to the activation of immune responses in the thrips.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Agronomy
Heron Delgado Kraide, Jorge Massaki Hasegawa, Julio Cesar Barbosa, Viviana Marcela Camelo-Garcia, Gabriel Madoglio Favara, Felipe Franco de Oliveira, Camila Geovana Ferro, Eike Yudi Nishimura Carmo, Elison Fabricio Bezerra Lima, Armando Bergamin Filho, Jorge Alberto Marques Rezende
Summary: Tomato crops in Brazil are affected by viral diseases such as ToSRV, ToCV, GRSV, and TCSV. Mixed infections can lead to synergistic interactions and resistance breakdown. This study evaluated the interaction between ToCV and orthotospoviruses in tomato plants with the Sw-5 gene, as well as the attractiveness of ToCV-infected plants to thrips. The results showed that ToCV pre-infection did not influence the effectiveness of the Sw-5 resistance gene against GRSV under field conditions.
Article
Entomology
Xiaobin Zheng, Yanran Wan, Min Tao, Jiangjiang Yuan, Kun Zhang, Jing Wang, Youjun Zhang, Pei Liang, Qingjun Wu
Summary: The Obst protein plays an important role in the acquisition and transmission of Tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis, while the ApoD and Orai proteins do not have this effect.
Article
Entomology
Peng Liang, Jie Ning, Wenlu Wang, Pu Zhu, Lianyou Gui, Wen Xie, Youjun Zhang
Summary: This study found that the antioxidant enzymes in Bemisia tabaci MED can help the insect adapt to long-term and short-term high temperatures by eliminating excessive reactive oxygen species (ROS).
Article
Entomology
Peng Wei, Xinying Zeng, Haonan Han, Yiqing Yang, Youjun Zhang, Lin He
Summary: Detoxification is crucial for agricultural pests to withstand pesticides, and cytochrome P450s, carboxyl/choline esterases (CCEs), and glutathione-S-transferases are responsible for this ability. Previous studies mainly focused on the effects of upregulation, downregulation, or mutation on CCEs, while the role of alternative splicing remains unclear. In this study, we identified two variants of TcCCE23 in Tetranychus cinnabarinus, and found that splicing affected the detoxification ability of TcCCE23. Overexpression of the spliced variant, CCE23-V2, resulted in higher mortality of mites under fenpropathrin exposure compared to the control group. Furthermore, molecular docking analysis suggested that splicing enlarged the substrate binding cavity of CCE23-V2, potentially enhancing its ability to accommodate fenpropathrin molecules. These findings indicate that splicing plays a significant role in enhancing the detoxifying capability of TcCCE23.
Article
Entomology
Kun Yang, Qin Peng-Hao, Meng-Ying Yuan, Lei Chen, You-Jun Zhang, Dong Chu
Summary: Communities of bacteria, especially symbionts, play a vital role in the growth and development of pests like Bemisia tabaci Mediterranean (MED), an invasive insect. This study investigated the infection density patterns of Cardinium in MED populations and the effects of high temperatures. Two isofemale lines from the same Chinese population and from other countries were examined using different techniques. The results revealed two different infection density patterns of Cardinium and showed that high temperature significantly affected the diversity and functions of bacterial communities in whiteflies with high-density Cardinium.
Article
Entomology
Jiaqi Zhu, Fu Wang, Youjun Zhang, Yuting Yang, Dengke Hua
Summary: Bradysia odoriphaga is a major insect pest in Asia that affects 30 commercial crops in seven plant families. The long-term use of chemical pesticides has led to resistance in these pests, as well as environmental issues and food contamination. In this study, the BodoOBP10 protein in B. odoriphaga was identified as playing a role in the recognition and localization of host plants. Silencing BodoOBP10 expression through RNA interference significantly reduced the insects' response to sulfur-containing compounds.
JOURNAL OF INSECT SCIENCE
(2023)
Letter
Entomology
Xingnuo Li, Peng Liang, Mingyue Wu, Shaoli Wang, Qingjun Wu, Wen Xie, Youjun Zhang
Article
Multidisciplinary Sciences
Zhaojiang Guo, Le Guo, Yang Bai, Shi Kang, Dan Sun, Jianying Qin, Fan Ye, Shaoli Wang, Qingjun Wu, Wen Xie, Xin Yang, Neil Crickmore, Xuguo Zhou, Youjun Zhang
Summary: In this study, the diamondback moth and its pathogen Bacillus thuringiensis were used to investigate the adaptive evolutionary mechanism. The host adaptation to the pathogen was associated with the insertion of a retrotransposon into the promoter of the MAP4K4 gene. This insertion enhanced the host defense mechanism against the pathogen by activating a hormone-modulated MAPK signaling cascade. This research provides new insights into the coevolutionary mechanism between host organisms and their microbial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Entomology
Hongran Li, Zhihui Jiang, Jincheng Zhou, Xin Liu, Youjun Zhang, Dong Chu
Summary: The study determined eight cryptic species in China and found that their distribution is related to geographical and environmental factors. It also found that the infection patterns of the whitefly and its endosymbionts are closely associated with ecological factors. This highlights the need to study the expansion of invasive species and host-endosymbiont interactions within an environmental and geographic context.
Article
Agronomy
Fengbo Yang, Haowei Shen, Tianyu Huang, Qixi Yao, Jinyu Hu, Juan Tang, Rong Zhang, Hong Tong, Qingjun Wu, Youjun Zhang, Qi Su
Summary: This study found that high levels of flavonoids in tomatoes enhance the plant's direct and indirect defenses against herbivores, increase resistance to pests, and attract predatory bugs. The high levels of flavonoids suppress the growth of herbivores and promote plant growth. Therefore, the use of transgenic plants can utilize the beneficial role of flavonoids in integrated pest management while maintaining or improving resistance against other pests and pathogens.
PEST MANAGEMENT SCIENCE
(2023)
Article
Genetics & Heredity
Xiang Wen, Kaiyang Feng, Juan Qin, Peng Wei, Peng Cao, Youjun Zhang, Zhiguang Yuchi, Lin He
Summary: This study reveals the mechanism by which a nuclear hormone receptor, TcHR96h, regulates the overexpression of the detoxification gene TcGSTm02 in response to cyflumetofen exposure. The study also shows that TcHR96h directly binds to cyflumetofen. These findings provide insights into the mechanisms of xenobiotic detoxification and metabolism in arthropods and are crucial for understanding adaptation in polyphagous herbivores.
Article
Horticulture
Yating Liu, Jinxi Xie, Wenlu Wang, Yanyuan Lei, Xuguo Zhou, Youjun Zhang, Wen Xie
Summary: The fruitless gene plays a key role in controlling sexual behavior in insects. In this study, two fru transcripts were identified and characterized in Bemisia tabaci. The results showed significant differences in the expression of fru-a and fru-b between females and males, which were also regulated by transcription factors.
Article
Agriculture, Multidisciplinary
Jinhang Li, Peng Wei, Juan Qin, Kaiyang Feng, Guangmao Shen, Wei Dou, Youjun Zhang, Peng Cao, Zhiguang Yuchi, Thomas Van Leeuwen, Lin He
Summary: Investigation found that acaricides inhibiting mitochondrial succinate dehydrogenase (SDHIs) have excellent control over phytophagous mites but limited toxicity to predatory mites and other beneficial organisms. The molecular mechanism of this selectivity is not fully understood. The main reason for this selectivity is that SDHIs have approximately 200-fold stronger inhibition on the SDH target in spider mites compared to predatory mites, indicating strong target-site selectivity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Horticulture
Jisong Zhang, Liping Huang, Zhuo Zhang, Zhanhong Zhang, Deyong Zhang, Youjun Zhang, Xiaobin Shi, Yong Liu
Summary: Insect vectors and insect-borne plant viruses pose a serious threat to agricultural production. Insecticides are commonly used to prevent the transmission of insect-borne viruses. However, the relationship between insect vector resistance and arboviruses has not been extensively studied. This study explores the effect of Tomato chlorosis virus (ToCV) on the insecticide resistance of Bemisia tabaci MED. The results show that ToCV infection upregulates detoxification-related genes and increases the activity of detoxification enzymes in B. tabaci, leading to reduced sensitivity to certain insecticides.
Article
Chemistry, Multidisciplinary
Zhaojiang Guo, Yang Bai, Xinyi Zhang, Le Guo, Liuhong Zhu, Dan Sun, Kaiyue Sun, Xudan Xu, Xin Yang, Wen Xie, Shaoli Wang, Qingjun Wu, Neil Crickmore, Xuguo Zhou, Youjun Zhang
Summary: This study reveals the role of N-6-methyladenosine (m(6)A) RNA modification in modulating the availability of juvenile hormone (JH) and its importance in host-pathogen interactions. It identifies the involvement of two m(6)A methyltransferase subunit genes in repressing the expression of a JH-degrading enzyme, leading to an increased JH titer and mitigating fitness costs associated with defense against the Bt pathogen.