Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 66-70Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.024
Keywords
Human immunodeficiency virus DNA; Cytosine-Ag+-cytosine coordination chemistry; Electrochemical DNA sensor; Exonuclease III-assisted target recycling; Immobilization-free
Categories
Funding
- National Natural Science Foundation of China [21405128, 81302696]
- National Science and Technology [2012ZX10004801-003-006]
- Military Science and Technology [AWS11C001]
- Research (Initial) Fund for the Doctoral Program of Xinxiang University (XRC)
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A new homogeneous electrochemical sensing strategy based on exonuclease III-assisted target recycling amplification was utilized for simple, rapid and highly sensitive detection of human immunodeficiency virus (HIV) DNA on an immobilization-free Ag(I)-assisted hairpin DNA through the cytosine-Ag+-cytosine coordination chemistry. The assay involved target-induced strand-displacement reaction accompanying dissociation of the chelated Ag+ in the hairpins and exonuclease III-triggered target recycling. Initially, the added target DNA hybridized with hairpin DNA to disrupt the Ag(I)-coordinated hairpin probe and releases the coordinated Ag+ ion. Then, the newly formed DNA double-stranded DNA could be cleaved by exonuclease III, and released target HIV DNA, which retriggered the strand-displacement reaction with the hairpin for target recycling, thereby resulting in formation of numerous free Ag+ ions in the detection cell. The released Ag+ ions can be readily captured by the negatively charged electrode, and subsequent anodic-stripping voltammetric detection of the captured Ag+ ions are conducted to form the anodic current for the production of the electronic signal within the applied potential. Under optimal conditions, the exonuclease III-based sensing system exhibited good electrochemical responses for the detection of HIV DNA at a concentration as low as 23 fM. (C) 2015 Elsevier B.V. All rights reserved.
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