Article
Biochemistry & Molecular Biology
Shao-Li Yang, Ngan Tran, Meng-Ying Tsai, Chin-Min Kimmy Ho
Summary: The research shows that the downregulation of MYB16 by SPCH in meristemoids is crucial for stomatal patterning, indicating the pivotal role of SPCH in the correct placement of polarity proteins essential for stomatal formation.
Article
Plant Sciences
Haodong Huang, Xianpeng Yang, Minglu Zheng, Shiyou Lu, Huayan Zhao
Summary: This study reveals the regulatory roles of KCS3 and KCS12 in cuticle biosynthesis. Mutations in KCS3 have a greater impact on wax production, while mutations in KCS12 exert more severe effects on cutin synthesis. KCS12 and KCS3 have additive effects on cuticle formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Paul Grunhofer, Tyll Stocker, Yayu Guo, Ruili Li, Jinxing Lin, Kosala Ranathunge, Heiko Schoof, Lukas Schreiber
Summary: In this study, the physiological effects of osmotic stress and salt treatment on poplar roots were compared. The results showed differences in the root responses to the two types of stress, with only osmotic stress leading to significant reductions in radial water transport. The findings suggest that enhancing root suberization processes could be a promising strategy for increasing tolerance in poplar, particularly against toxic sodium chloride.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Robert G. Orr, Fabienne Furt, Erin L. Warner, Erin M. Agar, Jennifer M. Garbarino, Sarah E. Cabral, Michelle L. Dubuke, Allison M. Butt, Mary Munson, Luis Vidali
Summary: The interaction between Rab-E and myosin XI is a conserved feature of polarised growth in plants, and it is essential for the normal plant growth. This interaction is specifically mediated by a certain residue in myosin XI, and it supports the spatial and temporal coupling of both proteins at sites of active exocytosis.
Article
Plant Sciences
Katsuaki Takechi, Hiroaki Nagase, Tomoyuki Furuya, Koro Hattori, Yoshikatsu Sato, Kensuke Miyajima, Tomofumi Higuchi, Ryuya Matsuda, Susumu Takio, Hirokazu Tsukaya, Hiroyoshi Takano
Summary: This study found that the PpAN2 genes play a crucial role in cell elongation regulation in moss plants, showing partial redundancy with the PpAN1 genes. Additionally, transgenic experiments revealed that the activity of AN is dependent on the N-terminal regions.
Article
Biochemistry & Molecular Biology
Yali Zhou, Xusheng Huang, Ting Hu, Shuwei Chen, Yao Wang, Xianfei Shi, Miao Yin, Runzhi Li, Jiping Wang, Xiaoyun Jia
Summary: This study identified PfGPAT9 as a key player in the biosynthesis and accumulation of seed oil and PUFAs in Perilla frutescens. Heterogeneous expression of PfGPAT9 in tobacco plants significantly increased total oil and unsaturated fatty acid levels without negative effects on other agronomic traits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Carolina Elejalde-Palmett, Ignacio Martinez San Segundo, Imene Garroum, Laurence Charrier, Damien De Bellis, Antonio Mucciolo, Aurore Guerault, Jie Liu, Viktoria Zeisler-Diehl, Asaph Aharoni, Lukas Schreiber, Benedicte Bakan, Mads H. Clausen, Markus Geisler, Christiane Nawrath
Summary: This study reveals the role of SlABCG42 in the formation of plant cutin, suggests that downregulation of certain tomato genes can affect cutin deposition and cuticle formation, and demonstrates the export activities of AtABCG32 and SlABCG42 for various cutin precursors in vivo.
Article
Plant Sciences
Shirsa Palit, Amey J. Bhide, Boominathan Mohanasundaram, Madhusmita Pala, Anjan K. Banerjee
Summary: In this study, the researchers investigated the role of a bryophyte-specific protein called SHORT-LEAF (SHLF) in plant development. They found that the signal peptide and the conserved tandem direct repeat (TDR) domains of SHLF are crucial for its function. SHLF acts as a secretory protein, and the TDRs contribute to the production of secreted peptides that are essential for its function. The researchers also discovered that the mutant form of SHLF lacks these peptides, and supplementation with the peptides or secretome from normal SHLF can partially or fully restore the mutant phenotype.
Article
Agricultural Engineering
Mathilde Marc, Regis Risani, Eric Desnoes, Xavier Falourd, Bruno Pontoire, Ruben Rodrigues, Rita Escorcio, Ana Paula Batista, Romain Valentin, Nathalie Gontard, Cristina Silva Pereira, Christelle Lopez, Eric Leroy, Denis Lourdin, Didier Marion, Benedicte Bakan
Summary: The study produced hydrophobic elastomers by mimicking the natural polyester networks of plant cuticles, using a sustainable process of polycondensation of glycerol and hydroxy fatty acids. The co-polyesters exhibited good functional properties and showed potential applications in antifouling due to reduced adhesion of bacterial cells.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Lin Zhang, Song Wang, Ji-Chang Han, Guan-Pin Yang, Ke-Hou Pan, Ji-Lin Xu
Summary: The study demonstrates that overexpression of AtDGAT in Nannochloropsis significantly increases lipid and TAG content without compromising algal growth. Enhancing TAG content through genetic engineering is a feasible and effective strategy for commercial biofuel production. This is the first successful attempt of plant-derived DGAT gene overexpression in Nannochloropsis, bringing novel insights into genetic manipulation of oleaginous microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Li -Li Liu, Yun-Quan Deng, Xin-Xiu Dong, Cheng-Feng Wang, Fang Yuan, Guo-Liang Han, Bao-Shan Wang
Summary: Ferulic acid plays an important role in the composition and structural properties of the cuticle, and its deficiency in cutin leads to reduced drought tolerance.
Article
Plant Sciences
Lei Shi, Yuqin Chen, Jun Hong, Gaodian Shen, Lukas Schreiber, Hagai Cohen, Dabing Zhang, Asaph Aharoni, Jianxin Shi
Summary: AtMYB31, a transcription factor that modulates wax biosynthesis, is involved in seed development in Arabidopsis.
Article
Biochemistry & Molecular Biology
Lucie Kriegshauser, Samuel Knosp, Etienne Grienenberger, Kanade Tatsumi, Desiree D. Guetle, Iben Sorensen, Laurence Herrgott, Julie Zumsteg, Jocelyn K. C. Rose, Ralf Reski, Daniele Werck-Reichhart, Hugues Renault
Summary: The HCT gene plays a critical role in the phenylpropanoid pathway in the moss species Physcomitrium patens, similar to its function in seed plants. Phylogenetic analysis suggests deep conservation of HCT function across embryophytes. Enzyme assays and metabolic profiling indicate that P. patens HCT is involved in the production of caffeate derivatives and cuticle formation in bryophytes.
Article
Multidisciplinary Sciences
Yanhua Ge, Yi Gao, Yuling Jiao, Ying Wang
Summary: This study reveals the regulation of midrib formation in moss by the orthologs of Arabidopsis LATERAL SUPPRESSOR gene, which is a key regulator of axillary meristem initiation in seed plants. The PpLAS/AtLAS genes have conserved functions in promoting cell division in both lineages, highlighting the importance of the conserved regulatory module in different developmental programs.
Article
Plant Sciences
Boominathan Mohanasundaram, Amey J. Bhide, Shirsa Palit, Gargi Chaturvedi, Maneesh Lingwan, Shyam Kumar Masakapalli, Anjan K. Banerjee
Summary: A novel bryophyte-specific gene, SHLF, was identified in this study, which affects leaf length and auxin distribution patterns in plants. Studies on the functional characteristics of SHLF and phylogenetic analysis could provide insights into the evolutionary adaptations of early land plants.
Article
Plant Sciences
Juyoung Kim, Ryeo Jin Kim, Saet Buyl Lee, Mi Chung Suh
Summary: Interactions between KCS9 and other proteins in the fatty acid elongase complex are essential for the synthesis of very-long-chain fatty acids (VLCFAs). This study provides insights into the quaternary structure and protein-protein interactions of the fatty acid elongase complex, highlighting their importance for efficient VLCFA synthesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Soohyun Oh, Doil Choi
Summary: Nonhost resistance is a plant immune response that prevents pathogenicity against the plant. The underlying molecular mechanisms of nonhost resistance are similar to host plant immune responses. The resistance is conferred by physicochemical barriers and defense responses induced by molecular recognition events. The relative contribution of each component may depend on evolutionary distances between plant species.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Plant Sciences
Mi Chung Suh, Hyun Uk Kim, Yuki Nakamura
Summary: Lipids are important biological molecules in plants, but their functions are still largely unknown compared to proteins and nucleic acids. Recent molecular biological approaches have revealed novel functions of lipids in plant function, and an increasing number of plant scientists are studying how lipids affect various aspects of plant function. This special issue provides a timely update from experts in the field, presenting cross-disciplinary discussions that explore new research angles on important plant physiological issues from the perspective of lipids.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Ryeo Jin Kim, Seat Buyl Lee, Garima Pandey, Mi Chung Suh
Summary: The formation of a hydrophobic cuticle layer on aerial plant parts played a critical role in protecting early land plants from the terrestrial environment. This study identified a transcriptional activator called PpWIN1, which is involved in the biosynthesis of cutin and cuticular wax. PpWIN1 was found to be highly expressed in specific plant parts and its overexpression resulted in increased levels of cutin and wax. This research provides important insights into the molecular mechanisms of cuticle biogenesis in early terrestrial plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Lei Jian, Kiyoon Kang, Yumin Choi, Mi Chung Suh, Nam-Chon Paek
Summary: The MYB transcription factor OsMYB60 positively regulates cuticular wax biosynthesis and contributes to drought tolerance in rice plants. The loss of function of OsMYB60 leads to downregulation of wax biosynthesis genes and reduces the amount of total wax on leaf surfaces.
Article
Plant Sciences
Ye-Eun Seo, Hye-Young Lee, Haeun Kim, Xin Yan, Sang A. Park, Myung-Shin Kim, Cecile Segonzac, Doil Choi, Hyunggon Mang
Summary: The Phytophthora capsici RxLR effector CRISIS2 triggers cell death by suppressing the plasma membrane proton pump in the host plant, thereby supporting the hemibiotrophic life cycle of the pathogen. CRISIS2 physically and functionally associated with PMAs and induced host cell death independent of immune receptors. This study identified a novel RxLR effector that plays multiple roles in the suppression of plant defense and in the induction of cell death to support the pathogen hemibiotrophic life cycle.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Ye-Eun Seo, Xin Yan, Doil Choi, Hyunggon Mang
Summary: Pathogens induce cell death in host plants for successful proliferation, and the regulation of plasma membrane H+-ATPases (PMAs) plays a crucial role in this process. This study reveals a mechanism by which pathogens manipulate PMA activity through interaction with host proteins, leading to cell death.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Biotechnology & Applied Microbiology
Soohyun Oh, Sejun Kim, Hyo-Jeong oPark, Myung-Shin Kim, Min-Ki Seo, Chih-Hang Wu, Hyun-Ah Lee, Hyun-Soon Kim, Sophien Kamoun, Doil Choi
Summary: In this study, multiple functional NLRs were identified from nonhost pepper that can recognize effectors of the potato late blight pathogen Phytophthora infestans and confer disease resistance in a surrogate system. These findings suggest that nonhost NLRs could be a valuable resource for developing crops with durable resistance against fast-evolving pathogens.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Semi Kim, Kyeong-Ryeol Lee, Mi Chung Suh
Summary: In this study, two isoforms of the WRI1 gene, PfWRI1A and PfWRI1B, were identified and found to be predominantly expressed in developing Perilla seeds. Ectopic expression of these isoforms significantly increased the levels of storage oils in tobacco leaves, particularly the polyunsaturated fatty acids. Furthermore, the expression of genes involved in fatty acid biosynthesis was also upregulated. These findings suggest that PfWRI1A and PfWRI1B have potential applications in enhancing the accumulation of storage oils with increased polyunsaturated fatty acids in oilseed crops.
Article
Multidisciplinary Sciences
Myung-Shin Kim, Seung-Won Lee, Kibum Kim, Yerin Kim, Hyunjoo Hwang, Peter Hinterdorfer, Doil Choi, Kisung Ko
Summary: Transgenic plants are a promising strategy for producing valuable biotherapeutic proteins, but optimization of codon sequences and expression cassette elements is necessary. This study utilized single-cell RNA sequencing to analyze the expression pattern of a llama antibody in transgenic tobacco leaves, providing a foundation for selecting the best cell types or tissues for high production.
Article
Plant Sciences
Soeui Lee, Hye-Young Lee, Hui Jeong Kang, Ye-Eun Seo, Joo Hyun Lee, Doil Choi
Summary: Transient and rapid increase in cytosolic Ca2+ is crucial for plant defense against pathogens. This study reveals that cyclic nucleotide-gated channels (CNGCs) play a key role in mediating Ca2+ influx during this process, and AVRblb2 interacts with CNGCs via calmodulin and calmodulin-like proteins. Moreover, Avrblb2 inhibits calcium channel activation by preventing the release of calmodulin and calmodulin-like proteins. The formation of active heteromeric channels with other CNGCs allows fine-tuning of cytosolic Ca2+ level for appropriate responses to diverse signals.
Review
Plant Sciences
Saet Buyl Lee, Mi Chung Suh
Summary: This study summarizes the regulatory mechanisms underlying cuticular wax biosynthesis in plants, including transcriptional, post-transcriptional, post-translational, and epigenetic levels. The advances in our understanding of these regulatory mechanisms have improved our knowledge of how plants balance carbon metabolic pathways between optimal growth and development and defense against abiotic stresses.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)