Article
Food Science & Technology
Zhendi Yu, Zhangkai Xu, Jiang Chen, Lili Chen, Ningbo Liao, Ronghua Zhang, Dongqing Cheng
Summary: Foodborne diseases are a critical food safety issue globally, with shellfish carrying various foodborne viruses posing a risk of infection. Factors such as age, season, and type of shellfish are associated with different levels of infection risk.
Article
Food Science & Technology
Pengzhen Li, Xiaoxuan Feng, Baiyan Chen, Xiaoying Wang, Zuyue Liang, Li Wang
Summary: A multiplex PCR system was developed for simultaneous detection of eight foodborne pathogenic bacteria. The system exhibited high sensitivity and specificity, and showed good stability and sensitivity in artificially contaminated samples.
Article
Food Science & Technology
Natharin Ngamwongsatit, Soraya Chaturongakul, Ratchaneewan Aunpad
Summary: Microbial contamination in foods can cause illnesses and significant losses in both the food industry and public health sectors. This study developed a multiplex PCR (m-PCR) assay for the rapid and reliable detection of six common foodborne pathogens and hygiene indicator microorganisms. The m-PCR showed comparable results to the gold-standard culture technique but had a significantly shorter processing time.
FOODBORNE PATHOGENS AND DISEASE
(2023)
Article
Food Science & Technology
Eun-Ji Shin, Soo-Jeong Lee, Hoon-Jae Jeong, Ju-Hoon Lee
Summary: A novel multiplex real-time PCR method was developed for rapid and accurate serogrouping of Escherichia coli O-serotypes, enabling identification in food environments.
Article
Chemistry, Analytical
Dechnarong Pimalai, Thitirat Putnin, Wassa Waiwinya, Chuleekorn Chotsuwan, Noppadol Aroonyadet, Deanpen Japrung
Summary: A highly sensitive electrochemical biosensor has been developed for the detection of multiplex micro ribonucleic acids (miRNAs), showing high selectivity, stability, and sensitivity. It is capable of detecting breast cancer biomarkers miRNA-155, miRNA-16, and miRNA-21 simultaneously, making it a potential tool for breast cancer screening in normal human serum.
Article
Biochemical Research Methods
Shiyu He, Yanzhi Huang, Yingwei Ma, Haoyan Yu, Bo Pang, Xingxing Liu, Caihong Yin, Xiaomu Wang, Yuan Wei, Yuling Tian, Chao Zhao, Kun Xu, Juan Wang, Chunping Lv, Xiuling Song, Minghua Jin
Summary: A new detection system using magnetic separation, MPCR, and CE technologies was developed to simultaneously detect four foodborne pathogens. The combination of magnetic separation, MPCR, and CE greatly increased detection sensitivity, with a detection limit for bacterial DNA of 10(-5)-10(-7)ng mu L-1. This technique showed good sensitivity and specificity in detecting bacteria in food samples, without the need for time-consuming enrichment cultures.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Ying Chen, Zixuan Wang, Qiaozhen Shi, Shengxiong Huang, Taotao Yu, Linyan Zhang, Huan Yang
Summary: This study presents a multiplex PCR method for detecting five food-relevant virulence pathogenicity genes of intestinal pathogens, offering a rapid and accurate approach for pathogen identification.
Article
Food Science & Technology
Lanxin Zhao, Xiaoyan Zhao, E. Hengchao, Tingting Fan, Enjing Tian, Chunling Wang, Yanmei Zhang, Xiaobei Li, Zhiyong Zhao, Changyan Zhou
Summary: A multiplex TaqMan real-time PCR assay was developed in this study for simultaneous detection of multiple highly toxic mushroom species, with the ability to specifically identify two specific highly toxic mushroom species in other mushroom types. The method had high sensitivity and was successfully applied to cooked mushrooms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Christopher Maier, Katharina Hofmann, Christopher Huptas, Siegfried Scherer, Mareike Wenning, Genia Luecking
Summary: Two triplex qPCR assays were developed for rapid detection and quantification of seven frequent and proteolytic Pseudomonas species in raw milk, along with a universal probe for total Pseudomonas counts. The assays showed high specificity and accurate quantification within linear detection ranges suitable for raw milk samples. Variability in species composition was observed in raw milk samples, with P. lundensis and P. proteolytica/P. gessardii being the predominant species detected.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Van-Giap Nguyen, Thi-Bich-Phuong Cao, Van-Truong Le, Ha-Thai Truong, Thi-Thanh-Huong Chu, Huu-Anh Dang, Thi-Hoa Nguyen, Thi-Luyen Le, Thi-My-Le Huynh
Summary: Infectious respiratory diseases in poultry can be caused by various pathogens and requires multiplex screening. In this study, a new multiplex PCR method was developed to detect Ornithobacterium rhinotracheale and infectious laryngotracheitis virus simultaneously.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
T. R. Kannaki, Priyanka Edigi, Nishitha Yalagandula, Santosh Haunshi
Summary: Multiplex PCR technique was developed and validated for simultaneous detection and differentiation of major tumor causing and immunosuppressive viral diseases of chicken, showing high sensitivity and specificity in detecting Marek's disease, avian leukosis, and Reticuloendotheliosis viruses.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Supaporn Wacharapluesadee, Piyapha Hirunpatrawong, Sininat Petcharat, Pattama Torvorapanit, Anusara Jitsatja, Nattakarn Thippamom, Sasiprapa Ninwattana, Chanchanit Phanlop, Rome Buathong, Ratanaporn Tangwangvivat, Chonticha Klungthong, Piyawan Chinnawirotpisan, Taweewun Hunsawong, Krairerk Suthum, Suparerk Komolsiri, Anthony R. Jones, Stefan Fernandez, Opass Putcharoen
Summary: The rapid emergence of highly severe and transmutable SARS-CoV-2 variants has led to an urgent need for rapid and multiplex molecular testing to identify these variants. A nucleotide matrix-assisted laser-desorption-ionization time-of-flight mass spectrophotometry (MALDI-TOF MS)-based assay called point mutation array (PMA) was developed to identify and differentiate major SARS-CoV-2 variants of concern (VOCs), including Alpha, Beta, Delta, and Omicron. The PMA panels showed high sensitivity in detecting VOCs and could determine co-infections or recombination of multiple variants.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Brigitte Sigrist, Jessica Geers, Sarah Albini, Dennis Rubbenstroth, Nina Wolfrum
Summary: Avian bornaviruses were first identified as causative agents of a disease in parrots in 2008. Currently, detection of these viruses is primarily done through RT-PCR, but existing methods have limitations in specificity and breadth of detection. A new multiplex real-time RT-PCR assay has been developed to overcome these limitations and simultaneously detect and differentiate avian bornaviruses.
Article
Chemistry, Analytical
Azusa Ooi, Eiji Tada
Summary: A channel-flow triple electrode (CFTE) was used to monitor the dissolution of Pt-Cu alloys during electrochemical measurements. It was found that Pt dissolution was below detection limit during anodic polarization, but both Pt and Cu dissolution were detected under potential cycling for a Pt-75 at% Cu alloy. The CFTE provides an excellent method for monitoring the dissolution of Pt-Cu alloys with high time-resolution.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Tohid Piri-Gharaghie, Ghazal Ghajari, Naz Tavakoli Lahijani, Renzon Daniel Cosme Pecho, Fahdil Hussam, Roxana Yolanda Castillo-Acobo, Mona Aghassizadeh-Sherbaf
Summary: This study aimed to develop a multiplex reverse transcriptase polymerase chain reaction (m-RT-PCR) method for simultaneous detection of important avian viral pathogens. Specific primers were designed and synthesized using conserved segments of viral genetic sequences, and recombinant vectors were synthesized as positive controls. The d-optimal approach was used to optimize PCR conditions. The m-PCR assay showed high specificity, sensitivity, and reproducibility in detecting and evaluating viral infection in birds, with the ability to detect 8 target genes and identify 8 viral pathogens from infected samples. The test also demonstrated that live animal oral swabs were as effective as tissue sampling from dead animals, making it a valuable tool for viral infection analysis in poultry.
Article
Chemistry, Analytical
Alejandra Ben Aissa, Narayanan Madaboosi, Mats Nilsson, Maria Isabel Pividori
Summary: This study utilizes isothermal amplification techniques for the detection of E. coli based on DNA, with preconcentration and amplification on magnetic particles. It shows improved analytical performance and eliminates the need for bulky equipment.
Article
Multidisciplinary Sciences
Patricia de Maria Silva Figueiredo, Jose Costa Sampaio Filho, Alzirene de Jesus Sales Sodre, Jose Ribamar de Castro Junior, Ingrid Santos Goncalves, Rodrigo Vieira Blasques, Rodrigo S. Correa, Benedicto Augusto Vieira Lima, Larissa dos Anjos Marques, Denise Fernandes Coutinho, Ana Paula Silva de Azevedo dos Santos, Tassio Romulo Silva Araujo Luz, Rita de Cassia Mendonca de Miranda, Julliana Ribeiro Alves dos Santos, Antonio Carlos Doriguetto, Maria Isabel Pividori, Manfredo Horner, Paulo Cesar Mendes Villis
Summary: In this study, novel triazene compounds were synthesized and tested for their antimicrobial and antifungal activities. The results demonstrated the potential of triazenes as agents against resistant bacterial and fungal strains, with promising activities observed against both gram-positive and gram-negative bacteria, as well as various fungi.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Juan Carlos Porras, Mireia Bernuz, Jennifer Marfa, Arnau Pallares-Rusinol, Merce Marti, Maria Isabel Pividori
Summary: Lateral flow assay (LFA) is a paper-based test designed for detecting specific analytes in low-resource settings. While colloidal gold is commonly used for detection, carbon nanoparticles (CNPs) have shown to be more sensitive, cost-effective, and stable, making them promising alternatives for improving the detection technology.
Review
Biochemical Research Methods
J. Marfa, R. R. Pupin, M. P. T. Sotomayor, M. I. Pividori
Summary: Magnetic particles and molecularly imprinted polymers have been widely utilized in scientific and technological applications, revolutionizing separation and bioanalytical methodologies; Hybrid molecularly imprinted polymers offer a cheaper and more stable alternative to biologically modified magnetic particles; The direct detection of electroactive analytes based on magnetic-MIP preconcentration shows potential in various fields such as food safety and environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xu Han, Gerard Boix, Mateusz Balcerzak, Oscar Hernando Moriones, Mary Cano-Sarabia, Pilar Cortes, Neus Bastus, Victor Puntes, Montserrat Llagostera, Inhar Imaz, Daniel Maspoch
Summary: This study reports the development of a metal-organic framework based composite for the controlled release of iodine, which exhibits antibacterial properties without causing drug resistance. The composite was successfully integrated into a polymer to create antibacterial films, which showed effective long-term release of iodine. In vitro tests confirmed the antibacterial activity of these films against gram-positive and gram-negative bacteria.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biophysics
Silio Lima Moura, Arnau Pallares-Rusinol, Luciano Sappia, Merce Marti, Maria Isabel Pividori
Summary: This article introduces a biosensor combining immunomagnetic separation and electrochemical biosensing based on the intrinsic ALP activity of exosomes. By improving the analytical performance for detecting ALP activity in exosomes, reliable differentiation between breast cancer patients and healthy individuals is achieved.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiaoqian Tang, Qi Zhang, Maria Isabel Pividori, Zhaowei Zhang, Jean-Louis Marty, Gaelle Catanante
Summary: Patulin contamination is a serious health risk factor for food safety and human health. In this study, a sensitive aptamer-based lateral flow assay was developed for rapid and simple patulin detection in apple juice.
Article
Environmental Sciences
Jesica M. Soder-Walz, Clara Torrento, Camelia Algora, Kenneth Wasmund, Pilar Cortes, Albert Soler, Teresa Vicent, Monica Rosell, Ernest Marco-Urrea
Summary: A novel Dehalobacter strain capable of transforming TCM to dichloromethane was obtained, along with its ability to transform other chlorinated compounds. Analysis of 16S rRNA genes confirmed growth of Dehalobacter with TCM and other chlorinated compounds as electron acceptors, and isotope fractionation during the transformation process was studied. These findings demonstrate a potential tool for differentiating TCM degraders in field studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Pedro Guirado, Elisenda Miro, Yaidelis Iglesias-Torrens, Ferran Navarro, Susana Campoy, Tyler Scott Alioto, Jessica Gomez-Garrido, Cristina Madrid, Carlos Balsalobre
Summary: This study describes the presence of a plasmid and its transferability in a multi-drug resistant strain of Campylobacter jejuni. The plasmid not only carries tetracycline resistance genes, but also a gene cluster that provides resistance to kanamycin and gentamycin. Additionally, the study suggests the possibility of genetic exchange between Campylobacter and distantly related G-positive bacterial genera.
Article
Chemistry, Analytical
Arnau Pallares-Rusinol, Silio Lima Moura, Merce Marti, Maria Isabel Pividori
Summary: This study developed a new method for the sensitive detection of cancer by preconcentrating exosomes released by tumor cells from serum using immunomagnetic separation and measuring GAPDH transcripts in exosomes using reverse transcription polymerase chain reaction and electrochemical genosensing. The application of this method in breast cancer cells and human serum samples showed high sensitivity and specificity, suggesting it as a promising strategy for minimal invasive liquid biopsy.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Amanda Cano, Miren Ettcheto, Mireia Bernuz, Raquel Puerta, Ester Esteban de Antonio, Elena Sanchez-Lopez, Eliana B. Souto, Antonio Camins, Merce Marti, Maria Isabel Pividori, Merce Boada, Agustin Ruiz
Summary: Extracellular vesicles are secreted by cells and play important roles in intercellular communication, immune responses, human reproduction, and synaptic plasticity. They have potential as biomarkers for diseases due to their biological relevance and easy accessibility. They can also be used as mirrors of brain diseases as they can cross the blood-brain barrier. This review explores their biogenesis, functions, and potential in clinical practice, as well as their role in major human brain diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Infectious Diseases
Maria Lajarin-Reinares, Eloy Pena-Rodriguez, Mariona Canellas-Santos, Elisabet Rosell-Vives, Pilar Cortes, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: Topically applied antimicrobial drugs have several advantages, but the widespread use of antibiotics has led to increasing antimicrobial resistance. Drug repurposing, such as with disulfiram, may provide an attractive alternative for treating multidrug-resistant bacteria in skin infections.
Article
Immunology
Julia Lopez-Perez, Jennifer Otero, Miquel Sanchez-Osuna, Ivan Erill, Pilar Cortes, Montserrat Llagostera
Summary: This study investigates the emergence of Salmonella strains with reduced susceptibility to a cocktail of three phages in different scenarios. The results show that the mechanisms of resistance vary in different environments, but they do not significantly impair the success of Salmonella biocontrol and oral phage therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
David Fernandez-Verdejo, Pilar Cortes, Albert Guisasola, Paqui Blanquez, Ernest Marco-Urrea
Summary: This study investigated the sequential transformation of chloroform to acetate using bioelectrochemical systems (BESs) and syntrophic dechlorinating cultures. The results showed the advantages of combining bacteria to fully detoxify chlorinated compounds in BESs, expanding the application of this technology for treating polluted water bodies.
ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY
(2022)
Article
Immunology
Jordi Corral, Maria Perez-Varela, Miquel Sanchez-Osuna, Pilar Cortes, Jordi Barbe, Jesus Aranda
Summary: Acinetobacter baumannii is a clinically significant pathogen with increasing worldwide importance, especially due to its ability to acquire resistance genes. This bacterium can move through two types of motility: twitching and surface-associated, with strain MAR002 exhibiting both. Mutants with altered motility patterns were identified through spontaneous rifampin-resistant mutations in the rpoB gene. Transcriptomic analysis revealed deregulated genes within each motility phenotype, highlighting the key role of surface-associated motility and limited role of twitching in the pathogenicity of A. baumannii strain MAR002.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
