Journal
NANO LETTERS
Volume 20, Issue 1, Pages 526-533Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b04263
Keywords
Nanoreactor; oxidative cancer therapy; enzyme reactions; glucose oxidase; enzymatic therapeutics; enzyme protection
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Funding
- Federal Ministry of Education and Research of Germany
- Max Planck Society
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In situ generation of anticancer agents at the place of the disease is a new paradigm for cancer therapy. The production of highly potent drugs by nanoreactors through a facile synthesis pathway is demanded. We report an oncolytic nanoreactor platform loaded with the enzyme glucose oxidase (GOX) to produce hydrogen peroxide. For the first time, we realized a core-shell structure with encapsulated GOX under mild synthetic conditions, which ensured high remaining activity of GOX inside of the nanoreactor. Moreover, the nanoreactor protected the loaded GOX from proteolysis and contributed to increased thermal stability of the enzyme. The nanoreactors were effectively taken up into different cancer cells, in which they produced hydrogen peroxide by consuming intracellular glucose and oxygen, thereby leading to effective death of the cancer cells. In summary, our robust nanoreactors are a promising platform for effective anticancer therapy and sustained enzyme utilization.
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