Review
Microbiology
Zhiyi Wang, Biao Chen, Tong Zhang, Guohui Zhou, Xin Yang
Summary: Rice stripe mosaic disease caused by rice stripe mosaic virus has become a significant rice disease in southern China. The virus is mainly spread by leafhoppers, causing symptoms such as yellow stripes, leaf distortion, and empty grains in rice plants. Researchers have made significant progress in understanding the disease's geographical distribution, symptoms, gene functions, and control measures.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Severine Lacombe, Martine Bangratz, Hoang Anh Ta, Thanh Duc Nguyen, Pascal Gantet, Christophe Brugidou
Summary: Rice ragged stunt virus (RRSV) is a destructive virus in the rice culture areas of South and Far-Eastern Asia, for which no genetic resistance has been identified. Chemical treatments are currently used, but RNA-silencing approaches have been developed as resistance strategies. While miRNA-based strategies targeting viral genomes did not achieve efficient resistance against RRSV, siRNA-based strategies targeting viral fragments showed efficient and environmentally friendly resistance in transgenic rice plants.
Article
Multidisciplinary Sciences
Kelvin T. Chiong, Will B. Cody, Herman B. Scholthof
Summary: This study demonstrates that using viral RNA suppressors can enhance the efficiency of CRISPR/Cas9 gene editing by regulating the expression of Cas9. Age was found to affect the efficacy of the RNA suppressor P19 during gene editing in plants. Co-delivery of Cas9 and sgRNA using a single viral vector can improve editing efficiency.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Qiang Ma, Fengmin Wang, Weiqi Song, Chaorui Huang, Kaili Xie, Zhongyan Wei, Yanjun Li, Jianping Chen, Hehong Zhang, Zongtao Sun
Summary: Plant auxin response factor OsARF17 plays a crucial role in plant defense against diverse rice viruses. Through comparative transcriptome analysis, it was found that down-regulated differentially expressed genes (DEGs) involved in plant-pathogen interaction and plant hormone signal transduction pathways were enriched in OsARF17 mutants under Rice stripe mosaic virus (RSMV) inoculation. The study suggests that OsARF17-mediated antiviral immunity is achieved by affecting the interaction between different phytohormones and regulating defense gene expression.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Dongsheng Jia, Guozhong Luo, Wei Shi, Ye Liu, Huan Liu, Xiaofeng Zhang, Taiyun Wei
Summary: Rice stripe mosaic virus (RSMV) and rice gall dwarf virus (RGDV) frequently co-infect rice plants in Guangdong province, China, and this co-infection has a synergistic effect on viral replication and pathogenicity. The co-infection also enhances the transmission of RSMV by the leafhopper vector, while having little effect on the transmission of RGDV. Moreover, non-viruliferous leafhoppers preferentially feed on co-infected rice plants, further enhancing viral transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Decai Tuo, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, Wenbin Liao, Di Sun, Xiaoying Li, Wentao Shen
Summary: Cassava is an important crop in least-developed and developing countries, and a CsCMV-based VIGS system was developed in this study, showing successful gene silencing in different cassava varieties and Nicotiana benthamiana, providing a new tool for gene function studies in cassava.
Article
Biotechnology & Applied Microbiology
Maria Luz Annacondia, German Martinez
Summary: RNA silencing plays a crucial role in mediating virus resistance in plants. The interaction between viruses and RNA silencing machinery is complex, involving the loading of viral small interfering RNAs (vsiRNAs) into host ARGONAUTE (AGO) proteins. This study shows that vsiRNAs from cucumber mosaic virus (CMV) can affect endogenous sRNA levels and displace other endogenous sRNAs when loaded into AGO proteins. Additionally, the viral suppressor of RNA silencing (VSR) 2b counteracts this by loading vsiRNAs and mRNA-derived siRNAs to affect gene expression.
Article
Plant Sciences
Ying Zhai, Anirban Roy, Hao Peng, Daniel L. Mullendore, Gurpreet Kaur, Bikash Mandal, Sunil Kumar Mukherjee, Hanu R. Pappu
Summary: This study identifies four silencing suppressors encoded by Croton yellow vein mosaic virus (CYVMV) and its cognate betasatellite, and investigates their subcellular localizations, interaction behavior, and roles in symptom induction and intercellular virus movement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Kira Tiedge, Janessa Destremps, Janet Solano-Sanchez, Magda Lisette Arce-Rodriguez, Philipp Zerbe
Summary: FoMV-mediated VIGS has been successfully achieved in both leaves and roots of switchgrass, providing an alternative approach for studying gene functions and physiological traits in this bioenergy crop.
Article
Biochemistry & Molecular Biology
Robert Pertermann, Ralph Peter Golbik, Selvaraj Tamilarasan, Torsten Gursinsky, Selma Gago-Zachert, Vitantonio Pantaleo, Iris Thondorf, Sven-Erik Behrens
Summary: Plant viruses can produce suppressor proteins that inhibit RNA silencing, a mechanism of antiviral plant immunity. These proteins can bind to virus-derived siRNAs and miRNAs, influencing cellular gene expression. The study found that a specific viral suppressor protein, p19, can bind to different miRNAs with varying affinities due to specific features in the RNA and protein molecules. These findings provide important insights into the efficiency and adaptability of the plant antiviral immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Linyu Liu, Haiyan Wang, Yan Fu, Wen Tang, Pingjuan Zhao, Yanli Ren, Zhixin Liu, Kunxin Wu, Xiuchun Zhang
Summary: The capsid protein of Turnip crinkle virus interacts with SGS3, enhancing viral accumulation and suppressing RNA silencing-mediated antiviral defense.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Weijun Cui, Shu Wang, Kelei Han, Ersong Zheng, Mengfei Ji, Binghua Chen, Xuming Wang, Jianping Chen, Fei Yan
Summary: The study revealed the important immune role of Ferredoxin 1 in plant virus infection and how viruses regulate its transcription levels through ABA and ABI5.
Article
Microbiology
Shusuke Kawakubo, Hangil Kim, Minoru Takeshita, Chikara Masuta
Summary: Host adaptation is a crucial factor in virus evolution, and this study reveals the host adaptation of Leek yellow stripe virus (LYSV) during its coevolution with Allium plants. LYSV accumulates abundantly in garlic and its relatives, but remains at a low level in leek. The viral P1 and HC-Pro proteins exhibit significant synergism in RSS activity in leek.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Shusuke Kawakubo, Hangil Kim, Minoru Takeshita, Chikara Masuta
Summary: This study investigates the host-adaptive evolution of leek yellow stripe virus (LYSV) through Bayesian phylogenetic comparative methods and a functional assay of viral RNA silencing suppressor (RSS) activity. It reveals that LYSV has evolved in response to different Allium plant hosts, with distinct evolutionary backgrounds between leek and garlic isolates. The research also demonstrates that viral RSS activity may play a crucial role in the host-specific adaptation of LYSV, specifically in leek.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Wenshuang Xie, Dee Marie Marty, Junhuan Xu, Nitika Khatri, Kristen Willie, Wanderson Bucker Moraes, Lucy R. Stewart
Summary: The results demonstrate that MDMV-based vector can be used as a tool for simultaneous gene expression and multi-gene silencing in maize.
Article
Plant Sciences
Yan Wang, Wei Dong, Qin Zhang, Zhen-Peng Li, Yue-Xing Wang, Xiu-Fen Li, Long-Jiang Huang
Summary: An efficient and scalable process for the synthesis of 19-hydroxyprogesterone was developed, achieving a total yield of 34.5%, which is significantly higher than the previously reported process (11.0% total yield). The plausible ring-opening mechanism of the 6,19-epoxy bridge in compound 7 was proposed, and the structures of intermediates were confirmed by LC-MS analysis.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2023)
Article
Environmental Sciences
Wei Huang, Guodong Cao, Chengliang Deng, Yanyan Chen, Tao Wang, Da Chen, Zongwei Cai
Summary: This study investigated the effects of TCS exposure on kidney function in mice. The results showed that TCS caused renal injury, oxidative stress, inflammation, and fibrosis in a dose-dependent manner. Metabolite analysis revealed lipid accumulation and defective fatty acid metabolism in TCS-exposed mouse kidney, which may contribute to renal function impairment.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Chemistry, Analytical
Akang Dan, Shasha Zhang, Zhongliang Chen, Jinghan Dong, Wenjun Zheng, Yuxin Tu, Zian Lin, Zongwei Cai
Summary: A simple and highly selective analytical method for phthalate monoesters (mPAEs) was developed using Cu2+-immobilized magnetic covalent organic frameworks. The method showed wide linear ranges, low limits of detection, and good correlation coefficients for the detection of mPAEs. The method was successfully applied to the sensitive detection of mPAEs in mouse plasma samples.
Article
Meteorology & Atmospheric Sciences
Jianguo Wu
Summary: This study used fuzzy set-based classification, Monte Carlo scheme, and climate shift data to determine the risk of reduction in distributional ranges of alpine graminoid species. The results showed that the main factor leading to range shifts was changes in the temperature index. Adaptation measures are needed to cope with future climatic conditions.
THEORETICAL AND APPLIED CLIMATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Huiyan Li, Huilin Zhang, Xiaofang He, Peiran Zhao, Tong Wu, Jinxuan Xiahou, Yelin Wu, Yanyan Liu, Yang Chen, Xingwu Jiang, Guanglei Lv, Zhenwei Yao, Jian Wu, Wenbo Bu
Summary: A communication interception strategy is developed to block spatiotemporal crosstalk between lysosomes and endoplasmic reticulum (ER) using amorphous-core@crystalline-shell Fe@Fe3O4 nanoparticles (ACFeNPs). This strategy induces autophagy and ER stress, leading to a significant therapeutic effect for cancer treatment.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Pengfei Hou, Yumiao Tian, Yu Xie, Fei Du, Gang Chen, Aleksandra Vojvodic, Jianzhong Wu, Xing Meng
Summary: This study develops a neural network potential (NNP) for aqueous MXene systems using deep neural networks and an active learning scheme, providing ab initio precision at low cost. The oxidation behaviors of super large aqueous MXene systems are investigated at nanosecond timescales, revealing the atomic-level process of MXenes oxidation and the inhibitory effects of free protons and oxides on subsequent oxidation reactions. These findings contribute to the development of effective protection strategies for controlling the stability of MXenes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Applied
Yinchun Fang, Jianguo Wu, Guojie Ma, Qufu Wei
Summary: Polylactic acid (PLA) fibre is a renewable and biodegradable synthetic polymer that has gained attention in textiles. However, its dyeing process faces challenges, and a waterless dyeing method using disperse dyes is being explored as an alternative. This study investigated the waterless dyeing of PLA fibre using different disperse dyes and decamethylcyclopentasiloxane (D5) as the medium. Optimal dyeing process conditions were determined, and it was found that disperse dyes with a monoazo structure were most suitable for PLA waterless dyeing. The research provides a basis for developing suitable dyes for waterless dyeing of PLA using D5.
COLORATION TECHNOLOGY
(2023)
Article
Environmental Sciences
Yan Ding, Dongqi Wang, Guanghui Zhao, Shu Chen, Taihu Sun, Hechen Sun, Chenyang Wu, Yizhe Li, Zhongjie Yu, Yu Li, Zhenlou Chen
Summary: Plant litter input is a crucial factor in the turnover of soil/sediment organic carbon (SOC). This study investigated the effects of leaf litter and stem litter input on SOC dynamics using the 13C isotope technique. It was found that both leaf and stem litter input facilitated SOC accumulation, with leaf litter having a higher contribution. However, leaf litter input also promoted SOC mineralization more than stem litter input. Overall, litter input led to a net increase in SOC accumulation but accelerated the loss of native SOC.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Biomedical
Yuanrong Li, Jianguo Wu, Chuanjiang He, Honghui He, Mingjun Xie, Ke Yao, Jing He, Yan Duan, Liujing Zhaung, Ping Wang, Yong He
Summary: Many mammalian tissues have specific cellular arrangements under stress stimulus, but conventional 3D encapsulation fails to replicate these arrangements accurately, so advanced cellular arrangement approaches are needed. In this study, a novel 3D prestress bioprinting approach is developed, using static sustained tensile stress, molecular chain orientation, and slow crosslinking in bioink to guide tissue construction. The semi-crosslinking state of the bioink provides excellent elasticity for applying stress on cells during the printing process. After printing, the bioink gradually crosslinks and maintains the applied stress force to induce cell-orientated growth. This approach allows for directional arrangement of multiple cell types, with adjustable internal stress in the hydrogel filament, and has potential applications in engineering skeletal muscles, tendons, ligaments, vascular networks, or combinations thereof.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Industrial
Ruiyu Xu, Song Huang, Zheren Song, Yuanyuan Gao, Jianguo Wu
Summary: This article proposes a deep mixed-effects modeling approach for anomaly detection in additive manufacturing. It captures the relationship between the melt pool temperature and other sensing data using a deep neural network, and incorporates random-effect and residual terms to account for variations and autocorrelations. Control charts based on T2 and generalized likelihood ratio statistics are developed for online monitoring.
Article
Environmental Sciences
Guanghui Zhao, Taihu Sun, Dongqi Wang, Shu Chen, Yan Ding, Yilan Li, Guitao Shi, Hechen Sun, Shengnan Wu, Yizhe Li, Chenyang Wu, Yufang Li, Zhongjie Yu, Zhenlou Chen
Summary: This study used stable isotopes to investigate the sources and transformation processes of nitrate in Suzhou Creek. The results showed that treated wastewater, soil nitrogen, and nitrogen fertilizer were the main sources of nitrate in the river. Reducing nitrate concentrations in treated wastewater is crucial for addressing nitrogen pollution in urban rivers.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Guodong Cao, Wei Wang, Jing Zhang, Pengfei Wu, Han Qiao, Huankai Li, Gefei Huang, Zhu Yang, Zongwei Cai
Summary: para-Phenylenediamine quinones (PPD-Qs) are newly discovered transformation products derived from para-phenylenediamine (PPD) antioxidants. Their occurrence and fate in wastewater treatment plants (WWTPs) need further investigation to improve pollutant removal efficiency.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Geochemistry & Geophysics
Imali Kaushalya Herath, Guitao Shi, Qian Zhao, Yilan Li, Danhe Wang, Su Jiang, Yangjie Li, Zhenlou Chen, Roberto Bargagli
Summary: This study analyzed the sources and cycling of nitrate (NO3-) in Antarctic soils. The research found that NO3- concentrations and isotopic values were consistent across the latitudinal gradient, with the majority of NO3- derived from nitrification process. Atmospheric deposition contributed minimally to the NO3- budget, and there was evidence of denitrification and cycling between NO3- and nitrite (NO2-). These findings provide new insights into the nitrogen cycle in Antarctic terrestrial ecosystems.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2023)
Review
Engineering, Biomedical
Haiyan Li, Keerthi Subramanian Iyer, Lei Bao, Jiali Zhai, Jiao Jiao Li
Summary: Granular microporous hydrogels made of non-spherical microgels show enhanced characteristics compared to traditional nanoporous hydrogels, promoting cell viability, migration, and tissue regeneration. These hydrogels have higher macroporosity and improved mechanical properties, allowing for better alignment and differentiation of cells in anisotropic tissue. The development of these hydrogels is gaining increasing interest in regenerative medicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Hui Chen, Cheng Jin, Li Xie, Jian Wu
Summary: Succinate, an intermediate of the TCA cycle, is crucial in mitochondrial metabolism, energy production, and is also considered a signaling molecule in metabolism and hepatic diseases. Its downstream signaling pathway through GPR91 leads to various intracellular responses, including succinylation, ROS production, HIF-1 alpha stabilization, and significant impact in cellular metabolism due to its role in the TCA cycle. Understanding the signaling mechanisms of succinate in hepatic fibrosis, metabolic reprogramming, inflammatory or immune responses, and carcinogenesis is of great interest. This review aims to provide the current understanding of succinate in mediating metabolism, inflammatory and immune responses in liver diseases, with the aim of establishing a molecular basis for therapeutic strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)