Article
Oncology
Dmitry Chistyakov, Mariia Guryleva, Elena S. Stepanova, Lyubov M. Makarenkova, Elena Ptitsyna, Sergei Goriainov, Arina Nikolskaya, Alina A. Astakhova, Anna S. Klimenko, Olga A. Bezborodova, Elena A. Rasskazova, Olga G. Potanina, Rimma A. Abramovich, Elena R. Nemtsova, Marina G. Sergeeva
Summary: This study investigates the alterations in oxylipin metabolism in breast cancer patients and identifies 18 differentially expressed oxylipins in the blood plasma of breast cancer patients at different stages. These oxylipin profiles could be used to evaluate the early stages of breast cancer.
Article
Biochemical Research Methods
Huifang Zhong, Qinggang Xie, Fang Li, Zhiyi Yang, Kaifeng Li, Qian Luo
Summary: This study developed a rapid, sensitive, and reproducible SPE-UPLC-MS/MS method for the comprehensive and specific quantification of oxylipins and their precursors in breast milk.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Mohd Abul Kalam, Muzaffar Iqbal, Abdullah Alshememry, Musaed Alkholief, Aws Alshamsan
Summary: A sensitive UPLC-MS/MS analytical method was developed and validated for the quantification of TDZ in rabbit?s aqueous humor. The method was linear in the concentration range, sensitive and reliable, and successfully employed for ocular pharmacokinetic study of TDZ after topical ocular application of TZP-containing formulations in rabbit eyes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Abdul-Aziz M. M. El-Attar, Ahmed A. Mohamed, Mohamed A. Hasan, Ahmed M. Abdel-Raoof
Summary: A highly sensitive, selective, accurate UPLC-MS/MS method was developed and validated for simultaneous quantification of azithromycin, tetracycline HCl, and diclofenac sodium in rabbit aqueous humor. Central composite design was used for extraction yield and LC condition optimization, achieving around 90% extraction efficiency under the best conditions.
MICROCHEMICAL JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Yunyun Zhan, Anzhou Wang, Yige Yu, Jie Chen, Xinhao Xu, Jingjing Nie, Jingjing Lin
Summary: This paper investigates the inhibitory effect of vortioxetine on cytochrome P450 and the type of inhibitory mechanism in human and rat liver microsomes. It reveals different types of inhibition in human and rat models and provides guidance for safe clinical use.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Food Science & Technology
Bianca Ferraz Teixeira, Fernanda Furlan Goncalves Dias, Thais Maria Ferreira de Souza Vieira, Juliana Maria Leite Nobrega de Moura Bell, Ameer Y. Taha
Summary: The study evaluated the impact of different extraction and base hydrolysis protocols on bound oxylipins in bovine milk, finding that 95% of oxylipins in milk were esterified, mostly derived from linoleic acid. Linoleic acid-derived metabolites were the most abundant oxylipin species in both free and bound lipid pools in milk.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Chemistry, Analytical
Mohd Abul Kalam, Sulaiman S. S. Alhudaithi, Adel Ali Alhowyan, Muzaffar Iqbal, Mohammad Raish, Abdullah K. K. Alshememry, Musaed Alkholief, Aliyah A. A. Almomen, Aws Alshamsan
Summary: A highly perceptive UPLC-MS/MS method was developed to quantitatively determine 5-FU in rabbit aqueous humor (AqH), using allopurinol as the internal standard. The method was validated and found to be rapid, sensitive, accurate, and capable of quantifying 5-FU in AqH. The method was applied to study the ocular pharmacokinetics of 5-FU in rabbits.
Article
Biology
Nadezhda V. Azbukina, Dmitry V. Chistyakov, Sergei V. Goriainov, Vladislav I. Kotelin, Elena V. Fedoseeva, Sergey Yu. Petrov, Marina G. Sergeeva, Elena N. Iomdina, Evgeni Yu. Zernii
Summary: The analysis of eye liquids from a cohort of primary open-angle glaucoma patients identified signaling lipids that suggest a role of arachidonic acid/platelet-activating factor (PAF)-dependent pathways and oxidative stress in the pathogenesis of the disease, providing novel targets for its diagnostics and treatment.
Article
Chemistry, Applied
Xiuli Jiang, Shihui Chen, Zili Guo, Dan Gu, Xianrui Liang
Summary: The study identified 25 compounds in Berchemia lineata extracts, including naphthopyrones and flavonoids, with weak inhibitory effects on CYP3A4 enzyme activity. The main metabolic pathway of the extracts' main component was found to be hydroxylation in its aglycone.
NATURAL PRODUCT RESEARCH
(2021)
Article
Immunology
Huiji Zhou, Luxian Zhou, Bo Li, Rongcai Yue
Summary: This study aimed to investigate the in vitro biological potency of Dendrobium officinale flowers (DOF) and screen its active components. The results showed that DOF extract has potential antioxidant capacity, anti-cyclooxygenase2 (COX-2) effect, anti-glycation potency, and anti-aging effects. A total of 34 compounds were identified using UPLC-ESI-QTOF-MS/MS, and the major potential antioxidants in DOF extract were determined through online ABTS radical analysis. Specific components in DOF and its active compound targeted related enzymes and highlighted their potential application in anti-aging.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Karolina Pietrowska, Diana Anna Dmuchowska, Adrian Godlewski, Emil Tomasz Grochowski, Malgorzata Wojnar, Wioleta Gosk, Joanna Konopinska, Adam Kretowski, Michal Ciborowski
Summary: The study aimed to quantitatively evaluate the symmetry in concentrations of different metabolites in aqueous humor. The comparison of both eyes revealed no significant differences in metabolite concentrations except for taurine. Most metabolites showed moderate-to-strong positive interocular correlation, indicating that a single eye could represent the fellow eye in terms of metabolite concentration.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Ophthalmology
Natsuka Kimura, Hidenori Takahashi, Shinichi Sakamoto, Yasuo Yanagi, Nozomi Maeshima, Ayaka Minamimoto, Noriko Iwamoto, Takashi Shimada, Ryozo Nagai, Kenichi Aizawa
Summary: This study developed a microvolume analytical method for measuring the aflibercept concentration in human intraocular fluid and plasma. By validating the method's accuracy and stability, it demonstrated the importance of reliable aflibercept analysis in human intraocular fluid.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Microbiology
Jun Ren, Menghuan Yu, Wenjin Gao, Chuanfan Ding, Shengjie Li, Shaoning Yu, Wenjun Cao
Summary: Prompt diagnosis and treatment of infective endophthalmitis are crucial. In this study, we investigated the feasibility of using Fc-MBL@Fe3O4 enrichment combined with MALDI-TOF MS profiling to identify pathogens in aqueous humor. We found that the enrichment efficiency of Fc-MBL@Fe3O4 was high, and identification could be completed within a short time, with a minimum bacterial concentration requirement of 2.20 x 10^6 CFU. For aqueous humor samples with initial bacterial counts in the tens to hundreds, bacterial identification through culture in micro-LB broth took 6.5 to 9.5 hours.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Mimi Sun, Mengmeng Yuan, Hong Wang, Runxin Yin, Chenjia Yan, Meihua Yin, Zenghu Su, Xinyu Lei, Xiangyu Fu, Senfeng Sun, Yazhuo Huang, Hongbo Xu, Shizhong Chen
Summary: In this study, an online system combining UPLC-PDA-ESI-Q-TOF-MS-LOX-FLD was developed for the identification and evaluation of anti-inflammatory active ingredients in Polygala tenuifolia Willd. A total of 101 compounds were identified, and 38 compounds showed strong LOX-binding activity. Through validation, 7 active compounds that combined with LOX to exert anti-inflammatory effects were identified for the first time.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Jinpeng Zhu, Xiaohong Kou, Caie Wu, Gongjian Fan, Tingting Li, Jinfeng Dou, Dongbei Shen
Summary: The ultrasound-assisted aqueous two-phase extraction (UA-ATPE) technique was used to extract flavonoids from jujube peels (JPs). The optimized extraction conditions were determined, and the extraction mechanism of UA-ATPE was explored.
Article
Medicine, Research & Experimental
Dmitry Kostyushev, Anastasiya Kostyusheva, Sergey Brezgin, Natalia Ponomareva, Natalia F. Zakirova, Aleksandra Egorshina, Dmitry V. Yanvarev, Ekaterina Bayurova, Anna Sudina, Irina Goptar, Anastasiya Nikiforova, Elena Dunaeva, Tatiana Lisitsa, Ivan Abramov, Anastasiia Frolova, Alexander Lukashev, Ilya Gordeychuk, Andrey A. Zamyatnin Jr, Alexander Ivanov, Vladimir Chulanov
Summary: CRISPR-Cas9 systems show remarkable anti-HBV activity by targeting the hepatitis B virus major genomic form, cccDNA. However, inactivating cccDNA alone is not enough to cure the infection, as HBV replication rebounds due to the de novo formation of cccDNA from its precursor, rcDNA. Depleting rcDNA before delivering CRISPR-Cas9 RNPs can prevent viral rebound and promote resolution of HBV infection. These findings lay the groundwork for developing a virological cure of HBV infection using a single dose of short-lived CRISPR-Cas9 RNPs, with the help of widely used reverse transcriptase inhibitors to block cccDNA replenishment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Pharmacology & Pharmacy
Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Alla Kondrashova, Anastasia Frolova, Olga Slatinskaya, Landysh Fatkhutdinova, Georgy Maksimov, Mikhail Zyuzin, Ilya Gordeychuk, Alexander Lukashev, Sergey Makarov, Alexander Ivanov, Andrey A. Zamyatnin Jr, Vladimir Chulanov, Alessandro Parodi, Dmitry Kostyushev
Summary: Biological nanoparticles, such as extracellular vesicles (EVs) and extracellular vesicle-mimetic nanovesicles (EMNVs), have been extensively studied as drug delivery vehicles in medical applications. This study investigated the properties and drug delivery abilities of EVs and EMNVs, and analyzed their use for delivering the chemotherapeutic drug doxorubicin. EMNVs were found to be more effectively internalized, while EVs showed higher intracellular release of doxorubicin.
Review
Biotechnology & Applied Microbiology
Sergey Brezgin, Alessandro Parodi, Anastasiya Kostyusheva, Natalia Ponomareva, Alexander Lukashev, Darina Sokolova, Vadim S. Pokrovsky, Olga Slatinskaya, Georgy Maksimov, Andrey A. Zamyatnin Jr, Vladimir Chulanov, Dmitry Kostyushev
Summary: Extracellular vesicles (EVs), cell-derived biological nanoparticles, have gained significant interest for drug delivery due to their ideal biocompatibility, safety, ability to cross biological barriers, and surface modification potential. However, the translation of EVs into practical applications has been challenging due to issues in up-scaling, synthesis, and quality control methods. Advances in manufacturing technology now allow EVs to carry various therapeutic cargoes, and new technologies have been introduced to improve EV production, isolation, characterization, and standardization.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Oncology
Mojdeh Soltani, Mustafa Ghanadian, Behrooz Ghezelbash, Abolfazl Shokouhi, Andrey A. A. Zamyatnin Jr, Alexandr V. V. Bazhin, Mazdak Ganjalikhani-Hakemi
Summary: Through this study, we found that PD-L1 stimulation can promote the proliferation and survival of AML stem cells, possibly through regulating the metabolic pathways of leukemic cells. PD-L1 stimulation affects glucose and fatty acid metabolism, increasing the expression of the pentose phosphate pathway and glycolysis in HL-60 and THP-1 cells, as well as increasing the expression of CPT1A while decreasing ACC1 expression.
Editorial Material
Oncology
Lyudmila V. Savvateeva, Evgeni Yu. Zernii, Andrey A. Zamyatnin
CURRENT CANCER DRUG TARGETS
(2023)
Review
Biochemistry & Molecular Biology
Larisa N. Ikryannikova, Neonila V. Gorokhovets, Darya A. Belykh, Leonid K. Kurbatov, Andrey A. Zamyatnin Jr
Summary: This article introduces the research on the relationship between bacteria and human tumors, including the efforts and achievements of ancient and modern scientists, as well as the challenges and achievements of 21st century science in using bacteria for cancer treatment. It also discusses the future possibilities of bacterial cancer therapy, including the creation of bacterial microrobots or bacteriobots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Viktoriia E. Baksheeva, Veronika V. Tiulina, Elena N. Iomdina, Sergey Yu. Petrov, Olga M. Filippova, Nina Yu. Kushnarevich, Elena A. Suleiman, Remi Eyraud, Francois Devred, Marina V. Serebryakova, Natalia G. Shebardina, Dmitry V. Chistyakov, Ivan I. Senin, Vladimir A. Mitkevich, Philipp O. Tsvetkov, Evgeni Yu. Zernii
Summary: Primary open-angle glaucoma (POAG) is a common neurodegenerative disorder that causes blindness and is characterized by optic nerve and retinal ganglion cell damage. Early detection and treatment of the disease are crucial, but difficult due to its asymptomatic nature and lack of objective diagnostic approaches. Recent studies have shown that glaucoma involves complex metabolomic and proteomic changes in eye liquids, including tear fluid. However, the analysis of tear fluid is technically challenging and not suitable for clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina P. Kolesova, Vera S. Egorova, Anastasiia O. Syrocheva, Anastasiia S. Frolova, Dmitry Kostyushev, Anastasiia Kostyusheva, Sergey Brezgin, Daria B. Trushina, Landysh Fatkhutdinova, Mikhail Zyuzin, Polina A. Demina, Evgeny V. Khaydukov, Andrey A. Zamyatnin, Alessandro Parodi
Summary: Proteolytic activity plays a crucial role in cell homeostasis and function, with implications in cancer progression and treatment response. Endosomes, which are often the final destination of internalized nanoparticles, are major sites of cellular proteolytic activity. However, little is known about the impact of nanoparticles on endosomal biology. In this study, we generated albumin nanoparticles with varying resistance to proteolysis and demonstrated a relationship between their sensitivity to proteases and drug delivery properties, irrespective of the degree of proteolytic degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Dmitry Kostyushev, Sergey Brezgin, Anastasiya Kostyusheva, Natalia Ponomareva, Ekaterina Bayurova, Natalia Zakirova, Alla Kondrashova, Irina Goptar, Anastasiya Nikiforova, Anna Sudina, Yurii Babin, Ilya Gordeychuk, Alexander Lukashev, Andrey A. Zamyatnin Jr, Alexander Ivanov, Vladimir Chulanov
Summary: APOBEC/AID cytidine deaminases play an important role in innate immunity and antiviral defenses. Researchers developed a CRISPR-activation-based approach to induce APOBEC/AID overexpression and control its effects on HBV replication and cellular toxicity. The study highlights the potential for precise control of APOBEC/AID activation as a strategy for suppressing HBV replication without toxicity.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Review
Biology
Anastasia S. S. Frolova, Olga E. E. Chepikova, Anna S. S. Deviataikina, Alena D. D. Solonkina, Andrey A. A. Zamyatnin
Summary: Cell death is an important process for multicellular organisms, and proteases play a crucial role in breaking down proteins and maintaining a healthy cellular environment. The activity of nuclear proteins determines the fate of the cell during certain types of cell death, making the regulation of nuclear proteases crucial. Understanding the roles and regulation of nuclear proteases in cell death could lead to new therapeutic targets for improving treatment outcomes.
Review
Biochemistry & Molecular Biology
Peter A. Ivanov, Tatiana V. Gasanova, Maria N. Repina, Andrey A. Zamyatnin Jr
Summary: Virus-specific proteins can trigger hypersensitive response (HR) in plants, which is a type of cell death. The main cell death signaling pathway involves the interaction between HR-inducing proteins and nucleotide-binding leucine-rich repeats (NLR) proteins encoded by plant resistance genes. NLR proteins can act as both sensors and helpers, or form an activation network leading to oligomerization and formation of membrane-associated resistosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Immunology
Jiazheng Sun, Andrey A. Zamyatnin, Xiangliang Yuan, Yi Xiao, Hongzhong Li
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Vera S. Egorova, Ekaterina P. Kolesova, Manu Lopus, Neng Yan, Alessandro Parodi, Andrey A. Zamyatnin
Summary: Cathepsin B is a lysosomal cysteine protease involved in various cellular processes and its overexpression and activity have been linked to pathological conditions such as cancer. Targeting cathepsin B activity has become a promising strategy for tumor-specific therapy and drug delivery. This review summarizes different technologies designed to improve cancer treatments by responsive to cathepsin B activity, which plays a key role in disease progression and treatment response.
Article
Biochemistry & Molecular Biology
Svetlana Yu. Maklakova, Anton V. Lopukhov, Alexandr D. Khudyakov, Sergey V. Kovalev, Maria P. Mazhuga, Olga E. Chepikova, Andrey A. Zamyatnin Jr, Alexander G. Majouga, Natalia L. Klyachko, Elena K. Beloglazkina
Summary: This study reports the synthesis and evaluation of atorvastatin conjugates targeting the galactose-specific hepatic asialoglycoprotein receptor (ASGPR). The prepared conjugates showed increased water solubility and demonstrated potent binding to the ASGPR. The conjugates with an amide bond displayed optimal stability and effectively inhibited HMG-R activity. These findings suggest that these compounds have the potential to be developed as orally administered prodrugs of atorvastatin.
RSC MEDICINAL CHEMISTRY
(2023)