Article
Biochemistry & Molecular Biology
Andressa Vilas Boas Nogueira, Marjan Nokhbehsaim, Anna Damanaki, Sigrun Eick, Christian Kirschneck, Agnes Schroeder, Jonathan Jantsch, James Deschner
Summary: The study revealed that the periodontopathogen F. alocis can stimulate the synthesis of visfatin and increase pro-inflammatory and proteolytic molecules in human macrophages via the TLR2 and MAPK pathways. Additionally, the pro-inflammatory cytokine TNF alpha demonstrates similar stimulatory effects on these molecules in macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Ezinne Aja, Arunima Mishra, Yuetan Dou, Hansel M. Fletcher
Summary: There is a direct correlation between environmental conditions, the oral microbial flora, and disease development in the periodontal pocket. Filifactor alocis, a significant organism in the pathogenic biofilm structure, exhibits pathogenic characteristics in the oxidative-stress microenvironment of the periodontal pocket. The characterization of FA519, a protein in F. alocis, suggests its involvement in oxidative stress resistance and modulation of important virulence attributes.
MICROBIOLOGY SPECTRUM
(2021)
Article
Cell Biology
Hyun Young Kim, Min-Kyoung Song, Yong Song Gho, Hong-Hee Kim, Bong-Kyu Choi
Summary: Periodontitis is an inflammatory disease caused by local infection in tooth-supporting tissue. While little is known about the causal effect of periodontitis on systemic bone resorption, this study found that extracellular vesicles from the periodontal pathogen Filifactor alocis induced bone loss and osteoclast differentiation via Toll-like receptor 2. Lipoproteins in the EVs were identified as responsible for the induced bone resorption.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Infectious Diseases
Rosa Romero-Martinez, Anushiravan Maher, Gerard Alvarez, Rui Figueiredo, Ruben Leon, Alexandre Arredondo
Summary: This study assessed the antimicrobial resistance profile of F. alocis and identified the presence of antimicrobial resistance genes. Six out of eight clinical isolates carried antimicrobial resistance genes, with five carrying tet(32) and one carrying erm(B). Overall, the isolates showed high susceptibility to the tested antibiotics except for the one carrying erm(B), which had lower susceptibility to azithromycin.
Article
Microbiology
Zeinab Razooqi, Carola Hoglund Aberg, Francis Kwamin, Rolf Claesson, Dorte Haubek, Jan Oscarsson, Anders Johansson
Summary: The study aimed to investigate the presence of two oral pathogens and their genotypes in relation to the development of periodontal disease. The results show that the presence of Aggregatibacter actinomyctemcomitans and Filifactor alocis is associated with increased periodontal pocket depth and progression of the disease.
Article
Dentistry, Oral Surgery & Medicine
Malissa Mangar, Arunima Mishra, Zhengrong Yang, Champion Deivanayagam, Hansel M. Fletcher
Summary: The survival and adaptation of Filifactor alocis in the inflammatory environment of the periodontal pocket require the ability to overcome oxidative stress. Its pathogenic characteristics include survival in the oxidative-stress microenvironment of the periodontal pocket and a probable ability to modulate microbial community dynamics. However, there is still a significant gap in our understanding of the mechanism of its oxidative stress resistance and its impact on biofilm virulence and pathogenicity.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Cell Biology
Marthe Jontvedt Jorgensen, Synne Jenum, Kristian Tonby, Rasmus Mortensen, Gerhard Walzl, Nelita Du Plessis, Anne Ma Dyrhol-Riise
Summary: The study found that cyclooxygenase-2 inhibitors can decrease the expression of monocytic MDSCs in the blood of tuberculosis patients, and reduce the production of specific immune regulatory factors in these cells. Additionally, cyclooxygenase-2 inhibitors also have an impact on mycobacterial infection and the function of monocytic MDSCs.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Arunima Mishra, Yuetan Dou, Charles Wang, Hansel M. Fletcher
Summary: This study found that Filifactor alocis can protect Porphyromonas gingivalis under oxidative stress conditions. The interaction between these two bacteria resulted in the modulation of several genes, including those involved in ABC transport and other cellular functions. These results suggest that F. alocis may stabilize the microbial community in the inflammatory microenvironment of the periodontal pocket by reducing oxidative stress.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cristina Cacheiro-Llaguno, Elena Hernandez-Subira, Manuel D. Diaz-Munoz, Manuel Fresno, Juan M. Serrador, Miguel A. Iniguez
Summary: This research investigates the inhibitory effect of dexamethasone on COX-2 transcriptional induction during T cell activation, revealing the immunomodulatory properties of glucocorticoids in human T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Longfei Xiao, Wanxu Sun, Yue Su, Ning Lu, Yanan He, Xihui Sheng, Xiaolong Qi, Kai Xing, Yong Guo, Di Chang, Xiangguo Wang, Junjin Zhao, Hemin Ni
Summary: In this study, we investigated the regulation of DHT on COX-2 expression and PGF2 alpha release in bovine EECs, finding that DHT dose-dependently increased COX-2 expression and PGF2 alpha release. This effect was mediated through AR-derived EGFR trans-activation and the PI3K/Akt cascade leading to NFkB activation, as confirmed by EGFR, PI3K/Akt, and NFkB inhibitors attenuating the DHT-mediated effect.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Piotr Wojcik, Michal Biernacki, Natalia Domian, Neven Zarkovic, Elzbieta Skrzydlewska
Summary: This study evaluated the impact of Celecoxib on apoptosis in keratinocytes, and the results suggested that COX-2 inhibition can attenuate UVB-induced apoptosis, with AEA playing a significant role as an anti-apoptotic agent in this process.
Article
Microbiology
Pruettichai Wisutep, Witchuda Kamolvit, Piriyaporn Chongtrakool, Anupop Jitmuang
Summary: This case report discusses a woman with a brain abscess in the left parietal lobe that presented as an atypical acute stroke-like syndrome. The pus samples from brain abscess aspiration revealed the presence of periodontal pathogens Porphyromonas gingivalis and Filifactor alocis. After receiving dental health care and 8 weeks of combined antimicrobial therapy, the patient made a full recovery.
Article
Biochemistry & Molecular Biology
Sonia Eligini, Susanna Colli, Aida Habib, Giancarlo Aldini, Alessandra Altomare, Cristina Banfi
Summary: Exposure of human endothelial cells to SIN-1 induced dose- and time-dependent expression of COX-2, leading to hypoglycosylation and reduced prostaglandin generation. This effect was specific to SIN-1 and involved de novo protein synthesis, impacting hexokinase activity and protein localization. The hypoglycosylated COX-2 showed altered degradation rates and may provide insights for potential pharmacological interventions to modulate COX-2 activity.
Review
Biochemistry & Molecular Biology
Agnieszka Kulesza, Leszek Paczek, Anna Burdzinska
Summary: The molecular mechanisms underlying the immunomodulatory properties of MSCs and the role of PGE2 and COX-2 in these properties are not fully understood. This article summarizes previous observations regarding the role of PGE2 and COX-2 in the immunomodulatory properties and other vital functions of MSCs. The most consistent results suggest that MSC-derived PGE2 inhibits the early maturation of dendritic cells, suppresses the proliferation of activated lymphocytes, and stimulates the differentiation of macrophages into M2 phenotype. COX-2/PGE2 also plays an important role in maintaining the basic life functions of MSCs and affects the formation of niches conducive to hematopoiesis and carcinogenesis.
Article
Environmental Sciences
Xiaoling Liu, Chunyan Yao, Yan Tang, Xiaoyan Liu, Chenggang Duan, Chunmei Wang, Fei Han, Ying Xiang, Long Wu, Yafei Li, Ailing Ji, Tongjian Cai
Summary: Excessive exposure to manganese may lead to neuroinflammation, and it can inhibit the expression of p53 through DNA methylation, further inducing the expression of COX-2 in microglial cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Correction
Dentistry, Oral Surgery & Medicine
Jens Weusmann, James Deschner, Jean-Claude Imber, Anna Damanaki, Natalia D. P. Leguizamon, Andressa V. B. Nogueira
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jens Weusmann, James Deschner, Jean-Claude Imber, Anna Damanaki, Natalia D. P. Leguizamon, Andressa V. B. Nogueira
Summary: The study found that glycine powder significantly increased the expression of proinflammatory genes and exploited the NF-kappa B pathway, while trehalose powder hardly interfered with cell function and did not trigger the NF-kappa B pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Birgit Rath-Deschner, Andressa V. B. Nogueira, Svenja Beisel-Memmert, Marjan Nokhbehsaim, Sigrun Eick, Joni A. Cirelli, James Deschner, Andreas Jaeger, Anna Damanaki
Summary: The study found that periodontitis and orthodontic tooth movement can increase the expression levels of IL-6 and CXCL2, with mechanical strain enhancing the stimulatory effect of Fusobacterium nucleatum on IL-6.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
N. Da Ponte Leguizamon, R. S. de Molo, G. Coletto-Nunes, A. V. B. Nogueira, S. Rocha, D. M. Neo-Justino, A. Soares-Costa, P. S. Cerri, U. H. Lerner, P. P. C. Souza, J. A. Cirelli
Summary: CsinCPI-2 inhibits inflammatory processes and alveolar bone loss caused by periodontal disease, as well as directly affecting osteoclastogenesis. In mice models, CsinCPI-2 significantly reduces inflammatory cell infiltration and prevents alveolar bone loss, offering potential for periodontal disease treatment.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Andressa V. B. Nogueira, Marjan Nokhbehsaim, Anna Damanaki, Sigrun Eick, Svenja Beisel-Memmert, Christian Kirschneck, Agnes Schroeder, Thamiris Cirelli, Natalia D. P. Leguizamon, Joni A. Cirelli, James Deschner
Summary: The impact of bacterial infection on the expression of growth hormone secretagogue receptor (GHS-R) was studied in periodontal cells and tissues, along with the evaluation of ghrelin. The results showed that bacterial infection upregulated GHS-R expression and protein level in periodontal tissues and human gingival fibroblasts (HGFs). Ghrelin was found to inhibit the proinflammatory effects of the bacteria on HGFs without affecting cell viability and proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Maria Eduarda Scordamaia Lopes, Camila Chierici Marcantonio, Rafael Scaf de Molon, Paulo Sergio Cerri, Cristiane Ribeiro Salmon, Luciana Souto Mofatto, Francisco Humberto Nociti Junior, James Deschner, Joni Augusto Cirelli, Andressa Vilas Boas Nogueira
Summary: The study found that obesity did not significantly impact bone loss resulting from periodontitis, but did affect the proteome of periodontal ligament tissues in experimental periodontitis, potentially identifying targets for personalized approaches.
JOURNAL OF PERIODONTAL RESEARCH
(2022)
Article
Infectious Diseases
Raluca Cosgarea, Christoph A. Ramseier, Soren Jepsen, Nicole Birgit Arweiler, Pia Merete Jervoe-Storm, Ionela Batori-Andronescu, Ralf Roessler, Torsten Conrad, Sigrun Eick, Anton Sculean
Summary: This study evaluated the effects of local drug delivery (LDD) or photodynamic therapy (PDT) as adjuncts to subgingival instrumentation (SI) in patients with persistent or recurrent periodontal pockets. The results showed that both LDD and PDT can significantly improve clinical, microbiological, and immunological outcomes in periodontal patients enrolled in supportive periodontal therapy (SPT) for up to 12 months, with no significant differences between the two treatments.
Article
Anatomy & Morphology
Jens Weusmann, James Deschner, Jean-Claude Imber, Anna Damanaki, Paulo Sergio Cerri, Natalia Leguizamon, Svenja Beisel-Memmert, Andressa Vilas Boas Nogueira
Summary: This study investigated the effects of a glycine powder and an erythritol/chlorhexidine (CHX) powder on human gingival fibroblasts (HGF). The results showed that both powders decreased cell viability and proliferation, and negatively influenced wound healing.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2022)
Article
Biochemical Research Methods
Camila Chierici Marcantonio, Maria Eduarda Scordamaia Lopes, Luciana Souto Mofatto, Cristiane Ribeiro Salmon, James Deschner, Francisco Humberto Nociti-Junior, Joni Augusto Cirelli, Andressa Vilas Boas Nogueira
Summary: This study aimed to evaluate the influence of obesity on the proteomic profile of periodontal ligament tissues during orthodontic tooth movement, identifying novel protein profiles associated with obesity. The results suggest that obese patients undergoing orthodontic treatment have distinct differences in the PDL proteome compared to healthy subjects. These findings provide valuable information for understanding tissue remodeling mechanisms in obese patients undergoing orthodontic treatment.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Andressa V. B. Nogueira, Maria Eduarda S. Lopes, Camila C. C. Marcantonio, Cristiane R. R. Salmon, Luciana S. S. Mofatto, James Deschner, Francisco H. H. Nociti-Junior, Joni A. A. Cirelli
Summary: This study aimed to evaluate the effects of obesity on the proteomic profile of the periodontal ligament in rats induced with a high-fat diet. The results showed differential expression of proteins between the obese and control groups, which were associated with obesity, suggesting that the periodontal ligament is responsive to obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna Damanaki, Svenja Beisel-Memmert, Marjan Nokhbehsaim, Ali Abedi, Birgit Rath-Deschner, Andressa V. B. Nogueira, James Deschner
Summary: The aim of this study was to investigate the effect of occlusal hypofunction on alveolar bone healing and the potential of enamel matrix derivative (EMD) for healing. A standardized fenestration defect was created in the mandibular first molar of Wistar rats, and occlusal hypofunction was induced. Regenerative therapy using EMD was applied to the defect. Three groups were established: normal occlusion without EMD treatment, occlusal hypofunction without EMD treatment, and occlusal hypofunction with EMD treatment. The results show that normal occlusal loading is beneficial for alveolar bone healing, while occlusal hypofunction delays bone regeneration. EMD partially compensates for the inhibitory effects of occlusal hypofunction on bone healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Pablo Cores Ziskoven, Andressa V. B. Nogueira, Lorena S. Gutierrez, Jens Weusmann, Sigrun Eick, Nurcan Buduneli, James Deschner
Summary: This study aimed to investigate the effects of Fusobacterium nucleatum on periodontal ligament (PDL) cells, and the modulatory role of apelin, to better understand the relationship between periodontitis and obesity. F. nucleatum increased the expressions of COX2, CCL2, and MMP1 in a dose- and time-dependent manner. The combination of F. nucleatum and apelin resulted in the highest expression levels of various inflammatory and tissue turnover molecules. F. nucleatum downregulated the expression of apelin and its receptor APJ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Egle Patrizia Milia, Luigi Sardellitti, Sigrun Eick
Summary: Pistacia lentiscus L. (PlL) has a long history of use in traditional medicine and its antimicrobial properties make it a potential alternative for oral infections. This review highlights the effectiveness of PlL polyphenol extracts in inhibiting periodontal pathogens and C. albicans, reducing inflammation, and preventing intraoral dysbiosis. The use of these extracts in toothpaste, mouthwashes, and local delivery devices could be beneficial for clinical management of oral diseases.