Article
Biochemistry & Molecular Biology
Marco D'Addio, Jasmin Frey, Carlotta Tacconi, Catharina D. Commerford, Cornelia Halin, Michael Detmar, Richard D. Cummings, Vivianne Otto
Summary: The interactions between sialoglycans on lymphatic endothelial cells (LEC) and macrophage-expressed Siglec-1 play a critical role in regulating macrophage localization and phenotype. The densely clustered, sialylated glycans on the SCS floor LEC were identified as a key component in the macrophage niche, providing anchorage for Siglec-1(+) LN-SCS macrophages. Defective binding of sialoglycans by Siglec-1 results in reduced numbers of Siglec-1(+) macrophages with a less proliferative and more anti-inflammatory phenotype.
Article
Biochemistry & Molecular Biology
Soran Mohammed, Natalie Ferry
Summary: The study compared the binding activity of mistletoe lectin isoform I (MLI) and Sambucus nigra agglutinin (SNA) towards sialic acid and beta-galactose through computational carbohydrate-protein docking and analysis of amino acid sequences, ultimately identifying their sialic acid binding domains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hirotaka Fudaba, Yasutomo Momii, Taisei Hirakawa, Kouhei Onishi, Daigo Asou, Wataru Matsushita, Yukari Kawasaki, Kenji Sugita, Minoru Fujiki
Summary: In this study, the expression of Siglec-15 on peritumoral macrophages was found to be significantly associated with longer overall survival in PCNSL patients, indicating a favorable outcome. Furthermore, Siglec-15 positivity in peritumoral macrophages and high-dose methotrexate-based chemotherapy were identified as independent predictors of overall survival.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Keyue Wu, Yiyang Li, Yuqi Lin, Baojun Xu, Jiajia Yang, Li Mo, Riming Huang, Xiaoyong Zhang
Summary: This study isolated and characterized a sulfated exopolysaccharide, AVP141-A, from the broth of marine-derived fungus. AVP141-A exhibited potential as an immune activator by significantly enhancing the activity of inflammation factors and activating specific signaling pathways in macrophages. These findings suggest that AVP141-A has the potential for further development as a marine fungal-derived therapeutic compound.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Su Ji Min, Heesu Lee, Myoung-Sook Shin, Jae Wook Lee
Summary: The study aimed to synthesize and analyze the anti-inflammatory effects of 23 coumarin derivatives on LPS-induced inflammation in RAW264.7 macrophages. Cytotoxicity tests showed that none of the compounds were toxic, and coumarin derivative 2 exhibited the highest anti-inflammatory activity by reducing nitric oxide production and suppressing proinflammatory cytokine expression. The compound also inhibited several key signaling pathways involved in inflammation. Overall, coumarin derivative 2 shows potential as an anti-inflammatory drug for the treatment of both acute and chronic inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Salvatore Desantis, Livio Galosi, Nicoletta Santamaria, Alessandra Roncarati, Lucia Biagini, Giacomo Rossi
Summary: This study examined the effects of multi-strain probiotics on the intestinal morphology and glycan composition of guinea fowl, demonstrating significant improvements in morphology and glycoprotein parameters in probiotic-treated group compared to control group. Probiotics have shown to induce region-specific changes in glycoprotein composition of the intestine, promoting gut health in poultry.
Article
Biochemistry & Molecular Biology
Lilli Arndt, Andreas Lindhorst, Julia Neugebauer, Anne Hoffmann, Constance Hobusch, Vasileia-Ismini Alexaki, Adhideb Ghosh, Matthias Blueher, Christian Wolfrum, Markus Glass, Martin Gericke
Summary: White adipose tissue fibrosis is strongly associated with inflammation and dysfunction due to obesity. IL-13 and IL-4 have been identified as critical mediators in fibrotic diseases, but their role in WAT fibrosis is still unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Florian Sicklinger, Ingmar Soren Meyer, Xue Li, Daniel Radtke, Severin Dicks, Moritz P. Kornadt, Christina Mertens, Julia K. Meier, Kory J. Lavine, Yunhang Zhang, Tim Christian Kuhn, Tobias Terzer, Jyoti Patel, Melanie Boerries, Gabriele Schramm, Norbert Frey, Hugo A. Katus, David Voehringer, Florian Leuschner
Summary: The study show that basophils play a crucial role in promoting tissue repair after myocardial infarction by increasing levels of IL-4 and IL-13 in the heart. Depletion of basophils leads to an increase in inflammatory response, scar thinning, and deterioration of cardiac function post-MI.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biology
Mimmi L. E. Lundahl, Morgane Mitermite, Dylan Gerard Ryan, Sarah Case, Niamh C. Williams, Ming Yang, Roisin Lynch, Eimear Lagan, Filipa M. Lebre, Aoife L. Gorman, Bojan Stojkovic, Adrian P. Bracken, Christian Frezza, Frederick J. Sheedy, Eoin M. Scanlan, Luke A. J. O'Neill, Stephen Gordon, Ed C. Lavelle, Simon Yona
Summary: This study provides new and unexpected insights into alternative macrophage activation states in the context of mycobacterial infection. Activation with IL-4 and IL-13 induces protective innate memory against mycobacterial challenge and enhances the killing capacity of macrophages. IL-4/13 trained macrophages retain heightened oxidative phosphorylation metabolism and impede heightened pro-inflammatory cytokine responses by inhibiting oxidative phosphorylation.
Article
Plant Sciences
Na-Ra Han, Kyeoung-Cheol Kim, Ju-Sung Kim, Seong-Gyu Ko, Hi-Joon Park, Phil-Dong Moon
Summary: The results of this study indicate that SH003 has immune-enhancing effects through the activation of macrophages, splenocytes, and NK cells, making it a potential immunostimulatory agent for diseases caused or exacerbated by immunodeficiency.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Dezhi Sui, Kaifan Liang, Yangxu Gui, Zhouchunxiao Du, Dongzhe Xin, Guojing Yu, Wantong Zhai, Xinrong Liu, Yanzhi Song, Yihui Deng
Summary: This study investigated the effects of SA derivatives on drug delivery systems, synthesizing four different SA derivatives and preparing SA derivative-modified liposomes. The results showed that an SA-cholesterol conjugate improved liposome stability, reduced plasma protein adsorption, and enhanced targeting of TAMs, leading to increased infiltration of CD8+ T cells in the tumor microenvironment. The findings provide valuable insights for research and development on SA-derivatives and offer a theoretical basis for clinical trials of SA-derivative-modified nanoparticles.
Review
Biochemistry & Molecular Biology
Anabel Gonzalez-Gil, T. August Li, Jean Kim, Ronald L. Schnaar
Summary: Most human Siglecs are expressed on immune cells and can down-regulate the immune response when binding to complementary sialoglycans. They have potential as drug targets for modifying inflammation and as immune checkpoints in cancer. Endogenous human sialoglycan immune regulators are being identified and may provide opportunities for therapeutic targeting of Siglecs.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Anabel Gonzalez-Gil, T. August Li, Jean Kim, Ronald L. Schnaar
Summary: Most human Siglecs are expressed on immune cells, carry inhibitory domains, and bind to endogenous sialoglycans. They have potential as drug targets for inflammation and cancer immunotherapy. The specific ligands for human Siglecs are starting to be identified, providing new therapeutic opportunities.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Article
Fisheries
Xiaohan Liu, Jiqing Gao, Xiao Wei, Xinze Zhang, Liping You, Yuanjin Liu, Lizhu Chen, Caili Liu, Chunxiao Sun, Xiuhui Tian, Quanli Zhou, Xiaomin Zhang
Summary: In this study, the potential mechanisms of PAMP binding and opsonic activities of a c-type lectin and a sialic acid-binding lectin from manila clam were investigated. The results showed that both lectins could bind to various pathogen-associated molecular patterns (PAMPs) and enhance the phagocytosis and encapsulation ability of hemocytes. These lectins may function as pattern recognition receptors (PRRs) involved in the innate immune responses of the clam.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Huiling Chen, Yanchao Zhang, Xing Li, Wei Zhang, Haoqi He, Bohai Du, Tianlan Li, Huanwen Tang, Yungang Liu, Li Li, Ming Shi
Summary: This study explores the in vitro toxicity of BPF on RAW264.7 macrophages. The results showed that a high concentration of BPF significantly reduced the survival rate of the macrophages, while a medium concentration caused apoptosis. Differential transcription analysis revealed changes in gene expression related to cell cycle, phagosome, lysosome, and antigen processing and presentation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima
Article
Multidisciplinary Sciences
Chika Yamashiro, Kotaro Sasaki, Yukihiro Yabuta, Yoji Kojima, Tomonori Nakamura, Ikuhiro Okamoto, Shihori Yokobayashi, Yusuke Murase, Yukiko Ishikura, Kenjiro Shirane, Hiroyuki Sasaki, Takuya Yamamoto, Mitinori Saitou
Article
Developmental Biology
Yuya Okuzaki, Hidenori Kaneoka, Takayuki Suzuki, Yota Hagihara, Yuki Nakayama, Seitaro Murakami, Yusuke Murase, Atsushi Kuroiwa, Shinji Iijima, Ken-ichi Nishijima
DEVELOPMENTAL BIOLOGY
(2019)
Article
Immunology
Hiroyuki Tateyama, Yusuke Murase, Hiroshi Higuchi, Yui Inasaka, Hidenori Kaneoka, Shinji Iijima, Ken-ichi Nishijima
Article
Developmental Biology
Yota Hagihara, Yuya Okuzaki, Kazuma Matsubayashi, Hidenori Kaneoka, Takayuki Suzuki, Shinji Iijima, Ken-ichi Nishijima
Article
Reproductive Biology
Hiroshi Ohta, Yukihiro Yabuta, Kazuki Kurimoto, Tomonori Nakamura, Yusuke Murase, Takuya Yamamoto, Mitinori Saitou
Summary: The study identified that forskolin and rolipram can enhance the proliferation/survival of mouse PGC-like cells, while cyclosporin A and fibroblast growth factors effectively improve the expansion of these cells in vitro. This improved culture system led to successful spermatogenesis and production of fertile offspring, offering insights into key questions in PGC biology such as germ cell migration, epigenetic reprogramming, and sex determination of the germline.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Yusuke Murase, Yukihiro Yabuta, Hiroshi Ohta, Chika Yamashiro, Tomonori Nakamura, Takuya Yamamoto, Mitinori Saitou
Article
Biotechnology & Applied Microbiology
Yusuke Kojima, Yuya Okuzaki, Ken-ichi Nishijima, Shuichiro Moriwaki, Seiya Asai, Hidenori Kaneoka, Shinji Iijima
Summary: By transferring the human erythropoietin gene under the control of a chicken lysozyme promoter-enhancer, efficient expression of the gene can be achieved in the oviduct. The -1.9 kb DHS plays a crucial role in oviduct-specific expression in genetically manipulated chickens, indicating that this approach can serve as an effective method for pharmaceutical production using transgenic chickens.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biology
Yoji Kojima, Chika Yamashiro, Yusuke Murase, Yukihiro Yabuta, Ikuhiro Okamoto, Chizuru Iwatani, Hideaki Tsuchiya, Masataka Nakaya, Tomoyuki Tsukiyama, Tomonori Nakamura, Takuya Yamamoto, Mitinori Saitou
Summary: The study identified a transcription factor circuitry that drives the germ line program, providing a paradigm for TF-based human gametogenesis.
LIFE SCIENCE ALLIANCE
(2021)
Article
Cell & Tissue Engineering
Yukiko Ishikura, Hiroshi Ohta, Takuya Sato, Yusuke Murase, Yukihiro Yabuta, Yoji Kojima, Chika Yamashiro, Tomonori Nakamura, Takuya Yamamoto, Takehiko Ogawa, Mitinori Saitou
Summary: This study successfully reconstituted the entire process of male germ-cell development using pluripotent stem cells (PSCs) in vitro, including primordial germ cell development, male germ-cell specification for spermatogonium development, and ensuing spermatogenesis. This creates a foundation for research on in vitro male gametogenesis.
Article
Cell Biology
Shihori Yokobayashi, Yukihiro Yabuta, Masato Nakagawa, Keisuke Okita, Bo Hu, Yusuke Murase, Tomonori Nakamura, Guillaume Bourque, Jacek Majewski, Takuya Yamamoto, Mitinori Saitou
Summary: The research demonstrates significant epigenomic heterogeneity in hiPSCs, with different germline potential correlating with X chromosome inactivation. This heterogeneity is primarily determined by genomic properties.
Article
Biochemistry & Molecular Biology
Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima, Ken-ichi Nishijima
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)