Journal
MACROMOLECULAR BIOSCIENCE
Volume 20, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201900312
Keywords
aligned nanofibrous membrane; electrospinning; scaffold; shape-memory; smooth muscle cells
Funding
- National Natural Science Foundation of China [31871864]
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Structural simulation of the smooth muscle layer plays an important role in tissue engineering of blood vessels for the replacement of damaged arteries. However, it is difficult to construct small-diameter tubular scaffolds to homogenously locate and align smooth muscle cells (SMCs). In this work, novel temperature responsive shape-memory scaffolds are designed for SMC culturing. The scaffolds are composed of an outer layer of poly(lactide-glycolide-trimethylene carbonate) (PLGATMC) for programming the deformation from planar to small-diameter tubular shape and an inner layer of aligned nanofibrous membrane of poly(lactide-glycolide)/chitosan (PLGA/CS) to regulate cell adhesion, proliferation, and morphology. The SMC behaviors and functions are dependent on the PLGA/CS ratios of membranes, and the scaffold with PLGA/CS 7:3 membrane exhibits the most suitable ability to regulate SMC behavior. The PLGA/CS@PLGATMC scaffold can be deformed into a temporary planar at 20 degrees C for convenient seeding and attachment of SMCs and then immediately self-rolled into 3D tube at 37 degrees C. The proposed strategy offers a practical approach for the development of small-diameter vascular scaffolds from 2D planar into 3D tubular shape by self-rolling.
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