Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 31, Issue 4, Pages 768-772Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.9b00097
Keywords
zebrafish; mass spectrometry imaging; cryosectioning; IR-MALDESI; lipids
Funding
- National Institutes of Health [R01GM087964]
- National Institute of Environmental Health Sciences, North Carolina State University, Center for Human Health and the Environment [P30ES025128, T32ES007046]
- Environmental Protection Agency [STAR 835541]
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The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease. In this work, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebrafish tissue sections. Detailed methods for overcoming the challenges of cryosectioning adult male zebrafish for MSI and complementary histological imaging are described. Representative two-dimensional ion maps demonstrated organ specific localization of lipid analytes allowing for visualization of areas of interest including the brain, liver, intestines, and skeletal muscle. A high resolving power mass spectrometer was utilized for accurate mass measurements, which permitted the use of open-source, web-based tools for MS1 annotations including METASPACE and METLIN. Whole-body MSI with IR-MALDESI allowed for broad lipid coverage with high spatial resolution, illustrating the potential of this technique for studying lipid-related diseases using zebrafish as a model organism.
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