Drop coating deposition Raman scattering of selected small molecules of biological importance

Drop coating deposition Raman scattering spectroscopy was used to study selected small biologically important molecules. This technique combines the advantages of low concentrations and small volumes of the initial aqueous solution. Detection limits were found to be 10(-6), 10(-7), and 10(-8) M deposition concentration for riboflavin, acetylsalicylic acid, and methylene blue, respectively. These detection limits are comparable or even better than those of surface-enhanced Raman scattering (SERS) spectroscopy using commercial Ag substrate.