Article
Orthopedics
L. A. Seewald, I. G. Sabino, K. L. Montney, M. L. Delco
Summary: This study evaluated the release of mitochondrial DNA (mtDNA) from injured chondrocytes and investigated the utility of synovial fluid mtDNA concentration in early detection of posttraumatic osteoarthritis. Results showed that chondrocytes released mtDNA following inflammatory and mechanical stress, and mtDNA concentration in synovial fluid increased in cases of joint injury. There was a strong positive correlation between cartilage damage and mtDNA concentration in naturally occurring posttraumatic osteoarthritis. Mitoprotective treatment mitigated impact-induced mtDNA release. Further research on mtDNA as a sensitive marker for early articular injury and response to mitoprotective therapy is warranted.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Cell Biology
Weizhou Jiang, Huan Liu, Rongxue Wan, Yanjiao Wu, Zhanjun Shi, Wenhua Huang
Summary: Mechanical loading is crucial for chondrocyte health, with mitochondria playing a critical role as mechanotransducers. Research has focused on the link between mechanical loading-induced mitochondrial dysfunction and the pathogenesis of osteoarthritis. There is a need for further investigation into the mechanisms behind mitochondrial functional alterations induced by mechanical stimulation.
AGEING RESEARCH REVIEWS
(2021)
Article
Multidisciplinary Sciences
Alireza Savadipour, Robert J. Nims, Neda Rashidi, Jaquelin M. Garcia- Castorena, Ruhang Tang, Gabrielle K. Marushack, Sara J. Oswald, Wolfgang B. Liedtke, Farshid Guilak
Summary: Through AFM and FEM studies, we found that PIEZO1 and PIEZO2 mediate calcium (Ca2+) signaling in chondrocytes at moderately high levels of cellular deformation, while at the highest strains, PIEZO1 functions independently of PIEZO2. Biophysical factors that increase apparent chondrocyte membrane tension also enhance PIEZO1-driven Ca2+ signaling. We identified a threshold of membrane stretch ratio that leads to PIEZO1-induced chondrocyte mechanical injury, suggesting potential targets for osteoarthritis prevention or treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Orthopedics
Steven Ayala, Michelle L. Delco, Lisa A. Fortier, Itai Cohen, Lawrence J. Bonassar
Summary: The study aimed to investigate whether post-injury articulation of cartilage exacerbates damage, and results showed that cartilage samples experiencing both impact and sliding suffered greater magnitude of damage across greater tissue depths.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Orthopedics
Xiaoxia Hao, Shengjie Wang, Jiaming Zhang, Tao Xu
Summary: PTOA is a subset of OA caused by joint injury, accounting for over 12% of cases. While exercise is recommended for OA patients, body weight-supported treadmill training has shown to be more efficient in slowing down the development of PTOA compared to traditional treadmill training.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Review
Immunology
James Orman Early, Lauren E. Fagan, Annie M. Curtis, Oran D. Kennedy
Summary: Inflammation is an important biological response to tissue damage, but dysregulation of the process can lead to tissue damage. Mitochondria play a key role in orchestrating the inflammatory response, beyond their traditional function in energy production. Osteoarthritis, previously thought of as non-inflammatory, now appears to involve chronic inflammation as well.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Orthopedics
Marysol Luna, Jason D. Guss, Laura S. Vasquez-Bolanos, Adrian J. Alepuz, Sophie Dornevil, Jasmin Strong, Denise Alabi, Qiaojuan Shi, Tania Pannellini, Miguel Otero, Ilana L. Brito, Marjolein C. H. van der Meulen, Steven R. Goldring, Christopher J. Hernandez
Summary: Obesity may not influence cartilage loss after a single mechanical insult without surgery/fracture, suggesting that damage to surrounding tissues or repeated mechanical insult is necessary for obesity to affect cartilage degeneration.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Review
Orthopedics
S. Oliveira, R. Andrade, F. S. Silva, J. Espregueira-Mendes, B. B. Hinckel, A. Leal, O. Carvalho
Summary: The study investigated the effects and mechanotransduction pathways of therapeutic ultrasound on chondrocytes. A total of 31 in vitro studies were included, and ultrasound was found to stimulate cartilage production and suppress degradation through activation or inhibition of different signaling pathways.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Biotechnology & Applied Microbiology
Paolo Caravaggi, Elisa Assirelli, Andrea Ensini, Maurizio Ortolani, Erminia Mariani, Alberto Leardini, Simona Neri, Claudio Belvedere
Summary: This study aims to investigate the impact of cyclic joint loading on cartilage metabolism, identify mechanisms for preventing or slowing down OA progression, and provide preliminary data on its application. The proposed protocol integrates biomechanical data, medical imaging, and molecular information to analyze human OA knee cartilage explants with different degrees of degeneration. The comprehensive analysis is expected to provide new insights on the beneficial effects of physiological loading and contribute to the design of non-pharmacological treatments for limiting OA progression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Virginia M. Stiffel, Charles H. Rundle, Matilda H-C Sheng, Subhashri Das, Kin-Hing William Lau
Summary: This study developed a therapeutic strategy for osteoarthritis (OA) based on EphA4 signaling. It demonstrated that the strategy of injecting EfnA4-fc can effectively prevent and treat OA/PTOA by reducing the destruction of articular cartilage and promoting regeneration.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Biology
Marc Sebastian Huppertz, Justus Schock, Karl Ludger Radke, Daniel Benjamin Abrar, Manuel Post, Christiane Kuhl, Daniel Truhn, Sven Nebelung
Summary: The study demonstrated that T2 mapping and texture feature analysis are sensitive diagnostic tools for detecting and monitoring post-traumatic degenerative changes in cartilage. These methods can be utilized to assess cartilage at risk after injury.
Article
Nanoscience & Nanotechnology
Pengfei Wang, Qingpeng Liu
Summary: This study explored the effect of curcumin nanoparticles combined with dexmedetomidine on cartilage injury in posttraumatic osteoarthritis (PTOA) rats. The results showed that the combination therapy reduced IL-1 beta and TNF-alpha levels, inhibited MMP activation, and exerted anti-inflammatory and analgesic effects. Dexmedetomidine also reduced pain response and inflammatory factor release. The combination of these two treatments can protect cartilage and delay the progression of PTOA.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Review
Rheumatology
Mercedes Fernandez-Moreno, Ignacio Rego-Perez, Francisco J. Blanco
Summary: This article summarizes the evidence that suggests osteoarthritis is a mitochondrial disease, emphasizing the important role of mitochondria in the pathogenesis of OA, such as regulating events like cell apoptosis, autophagy, senescence, and inflammation. Mitochondrial genetic variation may exert signaling control over the nuclear epigenome, modulating the development of OA.
CURRENT OPINION IN RHEUMATOLOGY
(2022)
Article
Orthopedics
Tristan Maerz, Michael D. Newton, Mackenzie Fleischer, Samantha E. Hartner, Karissa Gawronski, Lucas Junginger, Kevin C. Baker
Summary: The study found acute catabolic bone remodeling following ACL rupture, with thinning of subchondral bone in the medial femoral condyle and gains in the lateral femoral condyle. NIR imaging demonstrated immediate and sustained reduction of bone anabolism, with a significant increase in bone catabolism at 14 days.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Orthopedics
Parvathy Thampi, Suzanne M. Tabbaa, Brian Johnstone, Marcus A. Wimmer, Michel P. Laurent, C. Wayne McIlwraith, David D. Frisbie
Summary: The equine model of posttraumatic osteoarthritis mimics certain aspects of the naturally occurring disease, both in horses and humans. This study aimed to assess articular cartilage degeneration in a posttraumatic OA model using established scoring systems and a novel surface topography analysis. The results suggest that surface topography analysis may provide a reliable objective approach to assess early changes in the cartilage surface in OA.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Clinical Neurology
Sertac Kirnaz, Sunidhi Singh, Charisse Capadona, Marianne Lintz, Jacob L. Goldberg, Lynn B. McGrath, Branden Medary, Fabian Sommer, Lawrence J. Bonassar, Roger Hartl
Summary: Low back pain is often associated with degenerative disc disease, and biological treatment approaches like cell injections have shown promising results. However, challenges such as managing disc cells and the microenvironment of intervertebral discs need to be overcome for long-term safety and efficacy.
WORLD NEUROSURGERY
(2022)
Article
Multidisciplinary Sciences
Thomas Wyse Jackson, Jonathan Michel, Pancy Lwin, Lisa A. Fortier, Moumita Das, Lawrence J. Bonassar, Itai Cohen
Summary: This study presents experiments and theory that explain the structural origins of the shear properties of articular cartilage through the mechanical interdependence of collagen and aggrecan networks in its extracellular matrix. The study also highlights the importance of slight changes in collagen and aggrecan concentrations near the cartilage surface, which can significantly weaken its modulus and potentially lead to tissue collapse.
Article
Orthopedics
Marianne Lintz, Remy E. Walk, Simon Y. Tang, Lawrence J. Bonassar
Summary: Diabetes has been found to impact the biochemical composition, collagen fiber architecture, and mechanical behavior of intervertebral discs. Diabetic discs showed increased glycosaminoglycan and collagen content, as well as disorganized and unaligned collagen fibers at the boundary between the nucleus pulposus and annulus fibrosus. These changes made the diabetic discs stiffer and more resistant to deformation compared to wild-type discs. The findings suggest that diabetes may predispose individuals to degenerative disc disease later in life.
Article
Surgery
Nicholas A. Vernice, Sarah Caughey, Nabih Berri, Jason Harris, Alicia Matavosian, Xue Dong, Ryan J. Bender, Lawrence Bonassar, Jason A. Spector
Summary: This study explores the use of 3D-printed polylactic acid scaffolds and decellularized xenografts to bioengineer nipples in situ. The results demonstrate the maintenance of scaffold shape, diameter, and projection, as well as an increase in tissue volume. Histologic analyses reveal the preservation of native cartilage architecture and the invasion of fibrovascular tissue after 6 months. These findings support the potential use of decellularized allografts to enhance tissue ingrowth within a PLA scaffold for nipple reconstruction.
ANNALS OF PLASTIC SURGERY
(2022)
Article
Materials Science, Multidisciplinary
B. Kim, J. M. Middendorf, N. Diamantides, C. Dugopolski, S. Kennedy, E. Blahut, I Cohen, N. Bouklas, L. J. Bonassar
Summary: This study investigates the relationship between scaffold architecture and mechanical response in collagen scaffolds used for tissue-engineered cartilage. It demonstrates that the differences in mechanical response between scaffold architectures are primarily detected at the micro-scale due to the disparity in pore architecture. Confocal strain mapping combined with digital image correlation is critical for designing and optimizing architectures for porous materials.
EXPERIMENTAL MECHANICS
(2022)
Article
Orthopedics
Karan Vishwanath, Scott R. McClure, Lawrence J. Bonassar
Summary: This study characterized the lubricating ability of pAAm hydrogel and revealed its effectiveness in lubricating both native and degraded cartilage explants, suggesting an affinity for the articulating surface of the cartilage.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Engineering, Biomedical
Nicole Diamantides, Leigh Slyker, Sara Martin, Marcos R. Rodriguez, Lawrence J. Bonassar
Summary: This study aimed to investigate the effects of high sugar concentrations and extended glycation times on high concentration collagen pre-glycation. It was found that successful collagen gelation and rheological properties were strongly dependent on the ratio of added sugars to added AGEs.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Cell & Tissue Engineering
Xue Dong, Carly Askinas, Jongkil Kim, John E. Sherman, Lawrence J. Bonassar, Jason Spector
Summary: The challenge of limited auricular chondrocyte yield in clinical translation of tissue-engineered ear scaffolds for ear reconstruction was addressed by co-culturing human mesenchymal stem cells (hMSCs) with HAuCs to promote healthy elastic cartilage formation. The study successfully achieved shaped human elastic cartilage in external scaffolds by co-implanting hAuCs and hMSCs in collagen, highlighting a crucial step towards clinical translation of auricular tissue engineering.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Cameron Darkes-Burkey, Xiao Liu, Leigh Slyker, Jason Mulderrig, Wenyang Pan, Emmanuel P. Giannelis, Robert F. Shepherd, Lawrence J. Bonassar, Nikolaos Bouklas
Summary: This study demonstrates the development of stiff and tough biohybrid composites by combining collagen with a zwitterionic hydrogel through simple mixing. The resulting composite material exhibits mechanical properties similar to soft biological tissues such as articular cartilage. The addition of collagen increases the elastic modulus and toughness of the composite, while also improving cytocompatibility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Biomedical
Jongkil Kim, Lawrence J. Bonassar
Summary: This study demonstrates the profound effects of gelation pH on cellular activity, collagen fibril structure, and mechanical properties of collagen gels. Acidic and basic gelation pH resulted in cell death, while neutral pH showed a relatively high cell viability. Physiologic gelation pH displayed the greatest collagen deposition, and collagen fibril structure was affected by gelation pH. The gelation pH also influenced the equilibrium modulus of the gels.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Multidisciplinary Sciences
Jingyang Zheng, Thomas Wyse Jackson, Lisa A. Fortier, Lawrence J. Bonassar, Michelle L. Delco, Itai Cohen
Summary: Cellular response to stimulation plays a crucial role in tissue processes, and understanding how cells coordinate their response is essential. Researchers have developed a tool called STRAINS that combines fluorescent micrographs, cell tracking, and machine learning to measure the spatiotemporal distribution of cell behaviors in tissue. They used STRAINS to analyze the mechanotransduction response of chondrocytes in cartilage after mechanical injury, revealing distinct biochemical pathways and spatial patterns of cellular response.
Article
Orthopedics
L. A. Seewald, I. G. Sabino, K. L. Montney, M. L. Delco
Summary: This study evaluated the release of mitochondrial DNA (mtDNA) from injured chondrocytes and investigated the utility of synovial fluid mtDNA concentration in early detection of posttraumatic osteoarthritis. Results showed that chondrocytes released mtDNA following inflammatory and mechanical stress, and mtDNA concentration in synovial fluid increased in cases of joint injury. There was a strong positive correlation between cartilage damage and mtDNA concentration in naturally occurring posttraumatic osteoarthritis. Mitoprotective treatment mitigated impact-induced mtDNA release. Further research on mtDNA as a sensitive marker for early articular injury and response to mitoprotective therapy is warranted.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Materials Science, Biomaterials
Serafina G. Lopez, Jongkil Kim, Lara A. Estroff, Lawrence J. Bonassar
Summary: In this study, the researchers removed proteoglycans (GAGs) from collagen gel-based tissue engineered constructs using chondroitinase ABC (cABC) and found that it improved collagen fiber alignment and mechanical properties without compromising compressive strength. The increased fiber organization also appeared to prevent the propagation of large defects under loading. This study provides a new method of modulating the extracellular matrix for improved collagen fiber formation and mechanical properties in tissue engineered constructs.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Physics, Multidisciplinary
Jonathan Michel, Gabriel Von Kessel, Thomas Wyse Jackson, Lawrence J. Bonassar, Itai Cohen, Moumita Das
Summary: Previous studies have focused on spatially homogeneous variation in concentration while ignoring structural correlation. This study introduces a model in which dilute fiber networks are built in a correlated manner, enabling tissues to attain rigidity with less material. The study also finds that the percolation threshold varies nonmonotonically with the degree of correlation.
PHYSICAL REVIEW RESEARCH
(2022)
Meeting Abstract
Cell & Tissue Engineering
B. Kim, I. Cohen, L. Bonassar
TISSUE ENGINEERING PART A
(2022)