Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 172, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2020.107347
Keywords
Enterocytozoon hepatopenaei; Calcofluor white stain; Histopathology; Fluorescence
Categories
Funding
- Pilot National Laboratory for Marine Science and Technology (Qingdao) [QNLM201706]
- China Agriculture Research System [CARS-48]
- Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0502]
- Key Research and Development Project of Shandong Province, China [2019GNC106095]
- China Postdoctoral Science Foundation [2019M650170]
- Postdoctoral Applied Research Foundation of Qingdao (2019)
- National Key R&D Program of China [2019YFD0900101]
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A fluorescent stain, calcofluor white (CFW), was used for detection of spores of the microsporidium Enterocytozoon hepatopenaei (EHP). EHP spores in suspension, in feces, or in the infected hepatopancreas of the shrimp Penaeus vannamei, can be easily stained with this chitin and cellulose binding dye to show distinct blue-white fluorescent oval walls. The dye does not stain the host tissues. EHP spores showed orange-red spots by staining with hematoxylin and phloxine (H&P) in the section. CFW staining provides a simple and rapid method for determining the presence of EHP spores in fecal or tissue samples.
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