Journal
JOURNAL OF FISH DISEASES
Volume 43, Issue 5, Pages 531-540Publisher
WILEY
DOI: 10.1111/jfd.13146
Keywords
antiviral effect; aptamer; grouper iridovirus; Illicium verum Hook; f; extract
Funding
- Innovation-driven development special fund project of Guangxi [AA17204044, AA17204095-10]
- Key Research and Development Programs of Guangxi [2018AB52003]
- National Natural Science Foundation of China [41966004, 41706145]
- Natural Science Foundation of Guangxi Province [2019GXNSFAA245054, AD19245022]
- Basic Research Fund of Guangxi Academy of Sciences [2018YJJ903, 2019YJJ1005, 2018YJJ902]
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Grouper iridovirus causes high mortality rates in cultured groupers, and effective treatment for grouper iridovirus infection is urgently required. Illicium verum Hook. f. is a well-known medicinal plant with a variety of biological activities. The aim of this study was to analyse the use of I. verum extracts to treat grouper iridovirus infection. The safe working concentration of each I. verum extract was identified both in vitro and in vivo as follows: I. verum aqueous extract (IVAE) <= 500 mu g/ml; I. verum ethanol extract (IVEE) <= 250 mu g/ml; shikimic acid (SKA) <= 250 mu g/ml; trans-anethole (TAT) <= 800 mu g/ml; 3,4-dihydroxybenzoic acid (DDBA) <= 400 mu g/ml; and quercetin (QCE) <= 50 mu g/ml. The inhibitory activity of each I. verum extract against grouper iridovirus infection was analysed using aptamer (Q2)-based fluorescent molecular probe (Q2-AFMP) and RT-qPCR. All of the I. verum extracts displayed dose-dependent antiviral activities against grouper iridovirus. Based on the achieved per cent inhibition, IVAE, IVEE, DDBA and QCE were associated with the greatest antiviral activity (all > 90%). Together, our results indicate that I. verum extracts have effective antiviral properties, making it an excellent potential source material for the development of effective treatment for grouper iridovirus infection.
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