Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 122, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jcv.2019.104212
Keywords
HIV virus load; Point-of-Care technology
Categories
Funding
- Alere Inc. [FUJB 234/2016]
Ask authors/readers for more resources
Background: A new point-of-care (POC) HIV virus load technology has been recently developed and designed to be utilized in decentralized settings. Alere Technologies GmbH*, Germany, developed the mPIMA HIV-1/2 VL plasma WA which uses real time PCR technology with 50 mu l and a turnaround time of one hour. Objective: Analyze the performance of mPIMA to detect and quantify HIV-1 and HIV-2 and compare with Abbott M2000 assay fooling patients HIV-1 failing ARV therapy. Study design: In this study we evaluate the mPIMA HIV-1/2 VL plasma WA using 413 specimens from 270 patients failing ARV therapy, and compared its performance with Abbott RealTime HIV-1 Viral Load assay on the m2000 system. In addition, were determined VL in plasma specimens obtained from HIV-2 infected patients. Results: The results strongly indicate that mPIMA HIV-1/2 VL plasma WA can determine HIV-1 with concordance of 88.9 % (95 % CI 85.4-91.7) the reference WA when 1000 HIV-1 VL threshold was used as WHO cutoff to identify therapy failure. The overall correlation between HIV-1 VL was 0928 (Pearson correlation coefficient of Linear regression) and the Bland-Altman showed a mean difference of -0.20 Log cp/mL between the two technology. mPIMA HIV-1/2 VL plasma WA was also able to measure HIV-2 viral load in 16 specimens from Guinea-Bissau HIV-1/HIV-2 positive samples. Conclusions: These data support the use of mPIMA HIV-1/2 VL plasma WA to follow up patients and select patients failing ART, guiding immediate clinical decisions such as adherence counseling or ART regimen switch during the patient consultation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available