Journal
BRITISH JOURNAL OF PHARMACOLOGY
Volume 173, Issue 9, Pages 1478-1490Publisher
WILEY
DOI: 10.1111/bph.13444
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Funding
- Core Facility for Integrated Microscopy at University of Copenhagen
- People Programme (Marie Curie Actions) of European Union's Seventh Framework Programme under REA [608765]
- Lundbeck Foundation
- Novo Nordisk Foundation [107118]
- Medical Research Council [MR/K019074/1]
- British Heart Foundation [PG/12/63/29824]
- British Heart Foundation [PG/12/63/29824] Funding Source: researchfish
- Lundbeck Foundation [R181-2014-4030] Funding Source: researchfish
- Medical Research Council [MR/K019074/1] Funding Source: researchfish
- Novo Nordisk Fonden [NNF13OC0006553] Funding Source: researchfish
- MRC [MR/K019074/1] Funding Source: UKRI
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Background and PurposeKCNQ-encoded voltage-dependent potassium channels (K(v)7) are involved in the regulation of vascular tone. In this study we evaluated the influence of K(v)7 channel activation on smooth muscle relaxation in rat penile arteries and corpus cavernosum from normal and spontaneously hypertensive, heart failure-prone (SHHF) rats - a rat model of human metabolic syndrome. Experimental ApproachQuantitative PCR and immunohistochemistry were used to determine the expression of KCNQ isoforms in penile tissue. Isometric tension was measured in intracavernous arterial rings and corpus cavernosum strips isolated from normal and SHHF rats. Key ResultsTranscripts for KCNQ3, KCNQ4 and KCNQ5 were detected in penile arteries and corpus cavernosum. KCNQ1 was only found in corpus cavernosum. Immunofluorescence signals to K(v)7.4 and K(v)7.5 were found in penile arteries, penile veins and corpus cavernosum. The K(v)7.2-7.5 activators, ML213 and BMS204352, relaxed pre-contracted penile arteries and corpus cavernosum independently of nitric oxide synthase or endothelium-derived hyperpolarization. Relaxations to sildenafil, a PDE5 inhibitor, and sodium nitroprusside (SNP), an nitric oxide donor, were reduced by blocking K(v)7 channels with linopirdine in penile arteries and corpus cavernosum. In SHHF rat penile arteries and corpus cavernosum, relaxations to ML213 and BMS204352 were attenuated, and the blocking effect of linopirdine on sildenafil-induced and SNP-induced relaxations reduced. KCNQ3, KCNQ4 and KCNQ5 were down-regulated, and KCNQ1 was up-regulated in corpus cavernosum from SHHF rats. KCNQ1-5 transcripts remained unchanged in penile arteries from SHHF rats. Conclusions and ImplicationsThese data suggest that K(v)7 channels play a role in erectile function and contribute to the pathophysiology of erectile dysfunction, an early indicator of cardiovascular disease.
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