Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 24, Issue 4, Pages 2507-2518Publisher
WILEY
DOI: 10.1111/jcmm.14944
Keywords
HBV; HNF4 alpha; non-hepatic cells; NTCP; nuclear hormone receptors; PPAR alpha; RXR alpha
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Funding
- Natural Science Foundation of Guangdong province [2014A030313483, 2015A030313447]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
- National Nature Science Foundation of China [81571994, 81570567, 81870432]
- Li Ka Shing Shantou University Foundation
- Guangdong Government under the Top-tier University Development Scheme for Research and Control of Infectious Diseases
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Hepatitis B virus (HBV) is a human hepatotropic virus. However, HBV infection also occurs at extrahepatic sites, but the relevant host factors required for HBV infection in non-hepatic cells are only partially understood. In this article, a non-hepatic cell culture model is constructed by exogenous expression of four host genes (NTCP, HNF4 alpha, RXR alpha and PPAR alpha) in human non-hepatic 293T cells. This cell culture model supports HBV entry, transcription and replication, as evidenced by the detection of HBV pgRNA, HBV cccDNA, HBsAg, HBeAg, HBcAg and HBVDNA. Our results suggest that the above cellular factors may play a key role in HBV infection of non-hepatic cells. This model will facilitate the identification of host genes that support extrahepatic HBV infection.
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