Article
Biochemistry & Molecular Biology
Katia Todoerti, Maria Eugenia Gallo Cantafio, Manuela Oliverio, Giada Juli, Carmine Rocca, Rita Citraro, Pierfrancesco Tassone, Antonio Procopio, Giovambattista De Sarro, Antonino Neri, Giuseppe Viglietto, Nicola Amodio
Summary: Synthetic HTOL shows anti-tumor activity against multiple myeloma cells by reducing cell viability without affecting healthy cells, antagonizing key survival pathways for malignant plasma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Aya Fujikawa, Yasuhiro Ishihara, Emi Ohta, Tatsuo Nehira, Hisashi Mura, Mylene M. Uy, Shinji Ohta
Summary: Pimelocerus perforatus is a significant pest problem in olive cultivation in Japan. Two new racemic fluorescent benzoxazines, pimeforazine A and pimeforazine B, were isolated from P. perforatus. Their structures and absolute configurations were determined, and they were found to exhibit neuroprotective effects.
JOURNAL OF NATURAL PRODUCTS
(2022)
Review
Biochemistry & Molecular Biology
Doaa R. R. Adam, Manal M. M. Alem
Summary: Erectile dysfunction is a global public health concern associated with cardiovascular risk factors, and is considered a clinical marker for future adverse events.
Article
Food Science & Technology
Marta Gallardo-Fernandez, Ruth Hornedo-Ortega, Ana B. Cerezo, Ana M. Troncoso, M. Carmen Garcia-Parrilla
Summary: This study investigates the capacity of the metabolites DOPAL, MOPET, and MOPAL to prevent the aggregation and toxic effects of alpha syn fibrils. The results show that DOPAL exerts the greatest effect, preventing aggregation and alpha syn-induced neurotoxicity, and has a potent destabilising effect on the alpha syn fibrils. In addition, DOPAL can counteract the toxicity induced by alpha syn, possibly through the modulation of SIRT-2 and Hsp70.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Chemistry, Applied
Yanfang Lv, Qianqian Liang, Ying Li, Xinxin Liu, Defu Zhang, Xuepeng Li
Summary: This study investigated the interaction between Hydroxytyrosol (HT) and bovine serum albumin (BSA) in vitro, revealing that HT bound to the active site of BSA via hydrophobic forces, leading to conformational changes and fluorescence quenching. Higher concentrations of HT were found to increase the alpha-helix content of BSA.
FOOD HYDROCOLLOIDS
(2022)
Review
Chemistry, Medicinal
Heitor Gomes de Araujo-Filho, Jucilene F. dos Santos, Mikaella T. B. Carvalho, Laurent Picot, Ingrid Fruitier-Arnaudin, Hugo Groult, Lucindo J. Quintans-Junior, Jullyana S. S. Quintans
Summary: Limonene (LIM), a monoterpene abundant in Citrus fruits peels, has shown potential as a natural anticancer compound by inhibiting tumor initiation, growth, and angiogenesis, as well as inducing cancer cells apoptosis.
PHYTOTHERAPY RESEARCH
(2021)
Article
Chemistry, Medicinal
Andrea Spallarossa, Federica Rapetti, Maria Grazia Signorello, Camillo Rosano, Erika Iervasi, Marco Ponassi, Chiara Brullo
Summary: A library of novel derivatives of imidazo-pyrazole scaffold has been designed and synthesized with the goal of identifying potential antiproliferative/anti-inflammatory agents with multi-target mechanisms. These derivatives showed promising anticancer activity and antioxidant properties, and exhibited favorable drug-like and pharmacokinetic properties in in silico calculations.
Review
Pharmacology & Pharmacy
Bedanta Bhattacharjee, Ayesha Farhana Syeda, Damanbhalang Rynjah, Shalam M. Hussain, Shekhar Chandra Bora, Padmanath Pegu, Ram Kumar Sahu, Jiyauddin Khan
Summary: Head and neck squamous cell carcinoma is a deadly form of cancer that commonly occurs in the lining of epithelial cells. MicroRNAs play a critical role in the development and treatment of this cancer. This article emphasizes the molecular signaling pathways and discusses the potential of nanotechnology-based therapies.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Toxicology
Jaqueline Cristina Fernandes, Bruna Alves Fenerich, Antonio Bruno Alves-Silva, Natasha Peixoto Fonseca, Juan Luiz Coelho-Silva, Priscila Santos Scheucher, Eduardo Magalhaes Rego, Lorena Lobo Figueiredo-Pontes, Joao Agostinho Machado-Neto, Fabiola Traina
Summary: This study compared the potency and efficacy of three inhibitors of IGF1R-related pathways in a JAK2(V617F)-driven cellular model, finding that these inhibitors all have antineoplastic activity with NT157 being the most potent. The downregulation of the JAK2/STAT signaling pathway was a beneficial off-target effect of all three inhibitors.
TOXICOLOGY IN VITRO
(2022)
Review
Biochemistry & Molecular Biology
Anmol Sharma, Heena Khan, Thakur Gurjeet Singh, Amarjot Kaur Grewal, Agnieszka Najda, Malgorzata Kawecka-Radomska, Mohamed Kamel, Ahmed E. Altyar, Mohamed M. Abdel-Daim
Summary: The ubiquitin-proteasome pathway is crucial in regulating various biological functions and plays a significant role in tumor development. Inhibiting specific enzymes within this pathway can be an effective treatment for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ke Liu, Bixia Hong, Shuqi Wang, Fuxing Lou, Yecheng You, Ruolan Hu, Amna Shafqat, Huahao Fan, Yigang Tong
Summary: Cepharanthine, a natural bisbenzylisoquinoline alkaloid, is the only approved drug of its kind for human use. It has been used in the clinic for over 70 years and has various medicinal properties, including anti-tumor, anti-inflammatory, anti-pathogen activities, among others. Recently, it has been found to greatly inhibit SARS-CoV-2 infection. This article provides a comprehensive description of the pharmacological properties, mechanisms, safety, and potential clinical applications of cepharanthine, highlighting its potential as a drug for the treatment of different diseases.
Review
Pharmacology & Pharmacy
Huaguo Liang, Qingsong Huang, Li Zou, Peng Wei, Jiazheng Lu, Yongli Zhang
Summary: Methyl gallate (MG), a polyphenolic compound found in natural plants, has various biological functions, including anti-tumor, anti-inflammatory, anti-oxidant, neuroprotective, hepatoprotective, and anti-microbial activities, making it a promising candidate for further study and development.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Engineering, Chemical
Tania B. Ribeiro, Teresa Bonifacio-Lopes, Pilar Morais, Armenio Miranda, Joao Nunes, Antonio A. Vicente, Manuela Pintado
Summary: Incorporating liquid-enriched powder (LOPP) and pulp-enriched powder (POPP) from olive pomace into yoghurt can enhance its dietary fiber content, provide hydroxytyrosol and unsaturated fatty acids, and improve the lipids-phenolics interaction. The addition of POPP and olive oil positively influences the unsaturated fatty acids profile of yoghurts, while all olive pomace powder-fortified yoghurts exhibit higher total phenolic content and antioxidant activity compared to the control.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Food Science & Technology
Africa Fernandez-Prior, Alejandra Bermudez-Oria, Maria del Carmen Millan-Linares, Juan Fernandez-Bolanos, Juan Antonio Espejo-Calvo, Guillermo Rodriguez-Gutierrez
Summary: New liquid effluents based on the use of acetic acid in the table olive industry allow for easier extraction of bioactive compounds, particularly hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG). The anti-inflammatory activity of these phenolic extracts was demonstrated by studying the expression of cytokines and the levels of these proteins.
Review
Chemistry, Medicinal
Wen Hou, Wei Dai, Hao Huang, Sheng-Lan Liu, Jun Liu, Le-Jun Huang, Xian-Hua Huang, Jun-Lin Zeng, Zhi-Wei Gan, Zhen-Yu Zhang, Jin-Xia Lan
Summary: Heterocycles, such as pyrazine, are commonly found in clinically used drugs and possess enhanced target binding ability compared to alkanes due to their electron-accepting capacity and hydrogen bonding capability. In this review, we focus on the structure, in vitro and in vivo activity, and mechanisms of action of the most active pyrazine compounds, particularly those fused with other heterocycles, such as pyrrole or imidazole, which have been extensively studied for their antineoplastic activity. This review provides valuable information for the development of medications based on heterocyclic compounds, especially pyrazine.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Genetics & Heredity
Luigia Cinque, Lucia Micale, Elena Manara, Andrea Esposito, Orazio Palumbo, Andrea Maria Chiariello, Simona Bianco, Giulia Guerri, Matteo Bertelli, Maria Grazia Giuffrida, Laura Bernardini, Angelantonio Notarangelo, Mario Nicodemi, Marco Castori
Summary: Cooks syndrome is an ultra-rare limb malformation caused by tandem microduplications involving KCNJ2 and SOX9. A unique case associated with a de novo translocation affecting the KCNJ2 locus was reported, suggesting a complex rearrangement in this region may mimic the developmental consequences of in tandem duplications affecting the SOX9-KCNJ2 locus.
Article
Health Care Sciences & Services
Sandro Michelini, Karen L. Herbst, Vincenza Precone, Elena Manara, Giuseppe Marceddu, Astrit Dautaj, Paolo Enrico Maltese, Stefano Paolacci, Maria Rachele Ceccarini, Tommaso Beccari, Elisa Sorrentino, Barbara Aquilanti, Valeria Velluti, Giuseppina Matera, Lucilla Gagliardi, Giacinto Abele Donato Miggiano, Matteo Bertelli
Summary: The aim of this study was to design a next-generation sequencing (NGS) panel to help in the diagnosis of lipedema by identifying genes specific for lipedema but also genes for overlapping diseases, and targets for tailored treatments.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
Magdalena Ruskova, Maria Buckova, Adam Achs, Andrea Puskarova, Jer-Horng Wu, Tomas Kuchta, Zdeno Subr, Domenico Pangallo
Summary: This study selected an effective sample preparation procedure and combined it with a newly developed improved RT-PCR to detect viruses in wastewater samples. A model viral system was constructed using gene editing technology to evaluate the effectiveness of four viral RNA concentration/extraction methods. The research found that for wastewater samples with low viral loads, the OSN-qRT-PCR detection method performed the best and had the highest sensitivity.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2022)
Article
Food Science & Technology
Matej Plany, Jozef Sitarcik, Jelena Pavlovic, Jaroslav Budis, Janka Korenova, Tomas Kuchta, Domenico Pangallo
Summary: The use of third generation sequencing technologies allows for longer sequence reads, making it possible to use the longest amplicons as specific bacterial markers for metabarcoding studies. Nanopore sequencing technologies are increasingly used worldwide to analyze microbiomes in environmental and food samples. This study optimized a PCR-based method for sequencing almost the entire ribosomal operon sequences and applied it to the analysis of bacterial consortia in dairy fermented products.
Review
Biotechnology & Applied Microbiology
Patricia Sanmartin, Pilar Bosch-Roig, Domenico Pangallo, Lucia Krakova, Miguel Serrano
Summary: The organisms inhabiting built cultural heritage can passively or actively participate in conservation processes. Active participants are focused on for their positive roles in various processes, as well as providing ecosystem services. These organisms can also evolve or mutate in response to changes and acquire new capacities. Recent studies have opened up new avenues of research involving a wide variety of organisms and viruses, which are expected to gain more attention in the future.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Carmelo Gusmano, Rosita A. Condorelli, Andrea Bernini, Jurgen Kaftalli, Paolo Enrico Maltese, Stefano Paolacci, Astrit Dautaj, Giuseppe Marceddu, Matteo Bertelli, Sandro La Vignera, Aldo E. Calogero
Summary: This study investigated five unrelated patients with cHH/KS and identified a new pathogenic variant in the CHD7 gene and three new variants of unknown significance in the IL17RD, FGF17, and DUSP6 genes. Further functional studies are needed to confirm their association with cHH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Bence Farkas, Hana Vojtkova, Zuzana Farkas, Domenico Pangallo, Peter Kasak, Antonio Lupini, Hyunjung Kim, Martin Urik, Peter Matus
Summary: Manganese oxides are crucial in natural geochemical barriers, as they have reactive properties towards essential and potentially toxic trace elements. Microorganisms can actively alter conditions in their microenvironment and dissolve minerals, as well as precipitate bioavailable manganese ions into biogenic minerals. These microbial transformations significantly impact the geochemistry of manganese and elements associated with its oxides, potentially leading to environmental consequences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Ruskova, Alena Opalkova Siskova, Katarina Mosnackova, Custodia Gago, Adriana Guerreiro, Maria Buckova, Andrea Puskarova, Domenico Pangallo, Maria Dulce Antunes
Summary: This study focuses on encapsulating essential oils (lemongrass and oregano) into PLA and PHB packaging films and evaluating their antimicrobial and antioxidant properties for strawberry preservation and shelf-life extension. The results show that the tested active packaging film with essential oils can effectively maintain the quality and extend the shelf life of strawberries, with PLA/PHB/ATBC + 5% lemongrass essential oil performing slightly better than PLA/PHB/ATBC + 5% oregano essential oil.
Article
Medicine, General & Internal
Giorgio Placidi, Paolo Enrico Maltese, Maria Cristina Savastano, Elena D'Agostino, Valentina Cestrone, Matteo Bertelli, Pietro Chiurazzi, Martina Maceroni, Angelo Maria Minnella, Lucia Ziccardi, Vincenzo Parisi, Stanislao Rizzo, Benedetto Falsini
Summary: This study investigated the age-related changes and central retinal impairment in patients with EYS gene mutations. It found a positive correlation between RP stage scoring system (RP-SSS) and age, as well as a correlation between RP-SSS and central retina atrophy (CRA) area. Additionally, visual acuity and ellipsoid zone width were correlated with CRA, but not electroretinogram (ERG).
Article
Engineering, Biomedical
Maria Rachele Ceccarini, Francesca Ripanti, Veronica Raggi, Alessandro Paciaroni, Caterina Petrillo, Lucia Comez, Kevin Donato, Matteo Bertelli, Tommaso Beccari, Luca Valentini
Summary: In this study, adhesive patches made of silkworm-regenerated silk and DNA were developed to protect human skin from the sun's rays. The addition of DNA increased the crystallinity of silk fibroin (SF), as observed through infrared spectroscopy. UV-Visible absorption and circular dichroism spectroscopy confirmed the strong UV absorption and presence of B-form DNA in the SF matrix. Biological results showed that the SF/DNA patches increased the viability of keratinocytes after UV exposure. These patches have promising applications in wound dressing and biomedical purposes.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Genetics & Heredity
Francesca Cristofoli, Muharrem Daja, Paolo Enrico Maltese, Giulia Guerri, Benedetta Tanzi, Roberta Miotto, Gabriele Bonetti, Jan Miertus, Pietro Chiurazzi, Liborio Stuppia, Valentina Gatta, Stefano Cecchin, Matteo Bertelli, Giuseppe Marceddu
Summary: We have developed MAGI-ACMG, a classification algorithm that allows the classification of sequencing variants according to the recommendations of ACMG and ACGS. This algorithm utilizes the VarSome API, integrates the AutoPVS1 tool for precise evaluation, and performs customized assignment of criteria. Additionally, we propose a sub-classification scheme for variants of uncertain significance and introduce a pathogenicity potential criterion for upgrading clinical significance.
Article
Environmental Sciences
Magdalena Ruskova, Maria Buckova, Andrea Puskarova, Marianna Cichova, Veronika Janska, Adam Achs, Zdeno Subr, Tomas Kuchta, Domenico Pangallo
Summary: Wastewater monitoring is an important approach for detecting and controlling the SARS-CoV-2 pandemic. In this study, a one-step single-tube nested qRT-PCR assay was developed to increase the sensitivity of qRT-PCR. The assay demonstrated higher detection rates and robustness in samples with low viral loads.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Jelena Pavlovic, Zuzana Farkas, Lucia Krakova, Domenico Pangallo
Summary: Fungi are the main contaminants of books and archival documents, and they can degrade paper and produce pigments. Synthetic colors on paper can also be degraded by enzymes. This review explores the potential of enzymes in removing fungal and synthetic pigments, and discusses their potential applications.
APPLIED SCIENCES-BASEL
(2022)
Article
Psychiatry
Maria Rachele Ceccarini, Vincenza Precone, Elena Manara, Stefano Paolacci, Paolo Enrico Maltese, Valentina Benfatti, Kristjana Dhuli, Kevin Donato, Giulia Guerri, Giuseppe Marceddu, Pietro Chiurazzi, Laura Dalla Ragione, Tommaso Beccari, Matteo Bertelli
Summary: This study aimed to increase knowledge of genes associated with anorexia nervosa (AN) and their diagnostic offer using next generation sequencing (NGS). They found potentially deleterious variants in genes associated with syndromic forms of anorexia and identified candidate genes playing a possible causative role in AN etiology through family segregation studies. The study confirmed the involvement of the NNAT gene in the pathogenesis of AN and highlighted the importance of NGS-based testing in the diagnostic evaluation of AN.
EATING AND WEIGHT DISORDERS-STUDIES ON ANOREXIA BULIMIA AND OBESITY
(2022)
