Article
Green & Sustainable Science & Technology
Thomas Lendormi, Kais Jaziri, Fabrice Beline, Chrystelle Bureau, Cedric Midoux, Suzelle Barrington, Patrick Dabert, Sophie Le Roux
Summary: For small-scale farms, the development of rustic and cheap psychrophilic anaerobic digestion systems is an opportunity to treat manure, mitigate emissions, and promote renewable energy production. In this study, the most efficient inoculum for the start-up of psychrophilic anaerobic digestion of swine slurry at 13 ℃ was found to be swine manure stored for 2 months. After acclimation, the reactor produced a high methane yield, with a production efficiency of 55-68% at 13 ℃ compared to 37 ℃.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Environmental Sciences
Yura Jo, Roent Dune A. Cayetano, Gi-Beom Kim, Jungsu Park, Sang-Hyoun Kim
Summary: This study investigated the ammonia toxicity and acclimation of anaerobic microbiome during continuous anaerobic digestion of swine manure using unacclimated inoculum. The results showed that methane yield decreased significantly when ammonia level exceeded a certain concentration, but it was eventually recovered through further operation. Microbial analysis revealed shifts in both bacterial and archaeal populations under ammonia stress, with dominance of Clostridia in the recovered phase.
ENVIRONMENTAL RESEARCH
(2022)
Article
Microbiology
Ilayda Akacin, Seymanur Ersoy, Osman Doluca, Mine Gungormusler
Summary: This review provides a comprehensive overview of recent literature on the utilization of TGS and NGS technologies in microbial metagenomics research. It discusses the advantages and limitations of these technologies and presents real-time examples of novel applications in clinical microbiology and public health, food and agriculture, energy and environment, arts and space.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Environmental Sciences
Jiadong Yu, Lixin Zhao, Zonglu Yao, Jing Feng, Xufeng Yuan, Hongliang Wang, Yi Liang, Jiankun Chen, Yizhuo Du, Ruixia Shen
Summary: The study investigates the synergistic characteristics of abiotic and biotic transformation to improve the methane production efficiency in thermophilic and mesophilic sequencing batch dry anaerobic digestion (SBDAD). A pilot scale experiment using corn straw and cow dung as lignocellulosic material shows significant differences in biogas production and VFA concentration and composition. The combination of first-order hydrolysis and a modified Gompertz model increases cellulose and hemicellulose conversion efficiency by 112.03% and 90.09%, respectively, at thermophilic temperatures. The microbial community exhibits different functional network relationships under the two temperature conditions. Simulation of SBD-AD engineering shows reduced heat energy consumption and increased net energy production at thermophilic temperatures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Li-Jie Wu, Xiao-Xiao Li, Fan Yang, Quan Zhou, Rui-Peng Ren, Yong-Kang Lyu
Summary: Anaerobic digestion is a promising waste-to-energy technology, with hyperthermophilic and thermophilic microbial communities showing potential for efficiency improvement. Mesophilic digested sludge was used as inoculum, with hyperthermophilic digestion exhibiting higher hydrolysis ratio and thermophilic digestion showing higher methane proportion. Methanogenesis was found to be limited in the hyperthermophilic digestion, indicating the need for a pre-fermentation stage at 70 degrees C for enhanced hydrolysis.
Article
Microbiology
Indrani Mukhopadhya, Jennifer C. Martin, Sophie Shaw, Aileen J. McKinley, Silvia W. Gratz, Karen P. Scott
Summary: This study compares the bacterial profiles of faecal and mucosal microbiota and finds that faecal samples can be used as a convenient surrogate for rectal biopsy-associated microbiota. The results from bacterial culturing and 16S rRNA gene sequencing show similar taxonomic patterns.
Article
Biotechnology & Applied Microbiology
Jin Yeo, Chang-Gyu Kim, Jun-Hyeong Lee, Eunhye Song, Young-Man Yoon
Summary: This study examined the impact of water potential on anaerobic methane production and the microbial consortium. The water potential of the anaerobic digester ranged from -0.10 to -2.09 MPa, with an average of -1.23 MPa. The results showed that water potential was significantly correlated with various variables, and the methane production and utilization rates decreased as water potential decreased, with inhibition observed from approximately -1.65 MPa. At the microbial community level, the relative abundance of Methanosarcina decreased, while Methanoculleus significantly increased.
FERMENTATION-BASEL
(2023)
Article
Environmental Sciences
Bikram Basak, Swapnil M. Patil, Shouvik Saha, Mayur B. Kurade, Geon-Soo Ha, Sanjay P. Govindwar, Sean S. Lee, Soon Woong Chang, Woo Jin Chung, Byong-Hun Jeon
Summary: Acidification due to organic overloading is a major cause of process failures and decreased methane productivity in anaerobic digesters. This study demonstrates that bioaugmentation with acclimatized microbial consortium can effectively recover anaerobic digesters stalled due to acidosis and improve methane productivity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Harn Wei Kua, Xin He, Hailin Tian, Abhimanyu Goel, Tingting Xu, Wen Liu, Dingding Yao, Srikkanth Ramachandran, Xiao Liu, Yen Wah Tong, Yanjun Dai, Yinghong Peng, Chi-Hwa Wang
Summary: The potential of waste recovery strategies in reducing waste-related greenhouse gas emissions in Singapore was evaluated. The proposed strategies were found to be effective in reducing emissions from food, sewage, and plastic waste, with a significant reduction in greenhouse gas emissions compared to business-as-usual waste disposal methods.
RESOURCES CONSERVATION AND RECYCLING
(2022)
Article
Green & Sustainable Science & Technology
Abdul Haq, Ayesha Malik, Alam Khan, Joseph E. Weaver, Ling Wang, Haji Khan, Samiullah Khan, Aamer Ali Shah, Safia Ahmed, Asif Jamal, Francis L. de los Reyes III, Malik Badshah
Summary: The study shows that methanol-extracted residues from Jatropha curcas seeds can produce more biogas during anaerobic digestion, with a higher relative abundance of acetogenic bacteria. Additionally, anaerobic digestion of Jatropha seeds can achieve higher biogas yield at specific organic loading rates and hydraulic retention times.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Environmental Sciences
Parvin Hasani Zadeh, Antonio Serrano, Gavin Collins, Fernando G. Fermoso
Summary: The relationships between extracellular polymeric substances (EPS), soluble microbial product production, metal solubility, and methanogenic activity were investigated. Cobalt was found to be less toxic to methanogens than nickel, and the combined effects of Ni and Co on methane production in the bimetal experiment was higher than the sum of the effects of each metal alone. The protein content of EPS decreased with increasing concentrations of total metals, while no significant change was observed for carbohydrate content. Decreasing protein content of EPS was accompanied by reduced methanogenic activity and an increase in the soluble metal fraction.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Engineering, Environmental
Sameh Samir Ali, Haixin Jiao, Ahmed M. Mustafa, Eleni Koutra, Shimaa El-Sapagh, Michael Kornaros, Tamer Elsamahy, Maha Khalil, Laura Bulgariu, Jianzhong Sun
Summary: This study demonstrates the successful degradation of birchwood sawdust using a novel bacterial consortium SST-4, which can concurrently remove lignocellulose and creosote compounds, leading to efficient bioenergy production.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Ajay Thapa, Jeong-Hoon Park, Seung Gu Shin, Hong-Mok Jo, Min -Sang Kim, Yeongmi Park, Uijeong Han, Si-Kyung Cho
Summary: High-pressure anaerobic digestion (HPAD) is a promising technology for producing biogas enriched with high methane content. Experimental results showed that high pressure had a negligible effect on acidification, but significantly delayed methanogenesis. Microbial analysis revealed a shift in microbial community structure under high-pressure conditions. Several key microbial species were identified, including Methanobacterium, Methanomicrobiaceae, Alkaliphilus, and Petrimonas.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Eva Maria Prem, Mira Mutschlechner, Blaz Stres, Paul Illmer, Andreas Otto Wagner
Summary: Lignin intermediates may hinder anaerobic mineralisation, with phenyl acids serving as early indicators. The length and structure of side chains influence the methanogenic system, while specific microbial genera are associated with phenyl acid formation.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Environmental Sciences
Xiaowei Peng, Hong Su, Renjie Cai, Yejun Han
Summary: Microorganisms in marine sediments show promising potential for treating saline wastes due to their salt tolerance. The microbial communities in marine sediments have been found to exhibit excellent anaerobic digestion capabilities at various salinities, including high levels. The differences in bacterial taxonomic richness, nitrogen and sulfur cycle-related members, animal parasites, and archaeal compositions between marine sediment and wastewater plant inoculums highlight the unique microbial resources present in marine sediments for waste treatment applications.
ENVIRONMENTAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Xin Liu, Masaaki Fujiwara, Toshimitsu Kodera, Keiko Watanabe, Shinichi Akizuki, Masatoshi Kishi, Mitsuhiko Koyama, Tatsuki Toda, Syuhei Ban
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2020)
Article
Biotechnology & Applied Microbiology
Abd Wahab Farahin, Ikhsan Natrah, Norio Nagao, Fatimah Md Yusoff, Mohamed Shariff, Sanjoy Banerjee, Tomoyo Katayama, Masatoshi Nakakuni, Mitsuhiko Koyama, Kiyohiko Nakasaki, Tatsuki Toda
Summary: The study showed that Tetraselmis tetrathele has high tolerance to high concentrations of ammonium nitrogen and can continue to grow and increase production even at high levels.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shinichi Akizuki, Hiromi Joo, Mitsuhiko Koyama, Tatsuki Toda
Summary: This study found that deliberate starvation operation in an appropriate timeframe applied to a CSTR treating food waste is beneficial to proliferate cells and, at the same time, reduce residual substrate.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Environmental Sciences
Hapsari Puspitaloka, Hiroshi Mimoto, Quyen Ngoc Minh Tran, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: The study found that the organic matter degradation rate was faster under positive aeration, possibly due to better heat retention. Despite different temperatures and organic matter degradation rates, the microbial community involved in degrading easily degradable material was similar in both aeration types. Predicted key functional genes showed that protease gene abundance was similar in positive and negative aeration, indicating that high temperatures in positive aeration accelerated enzymatic activity without affecting microbial community or catabolic function.
WASTE AND BIOMASS VALORIZATION
(2022)
Article
Biotechnology & Applied Microbiology
Syazni Zainul Kamal, Quyen Ngoc Minh Tran, Mitsuhiko Koyama, Hiroshi Mimoto, Chikako Asada, Yoshitoshi Nakamura, Kiyohiko Nakasaki
Summary: Hydrothermal treatment (HTT) as a pretreatment for compost raw material has shown positive effects on food waste composting, including enhanced solubility of organic material, improved bioaugmentation, and reduced biohazard. HTT pretreatment resulted in less organic matter degradation and lower compost phytotoxicity compared to non-HTT-pretreated compost. High-throughput microbial community analysis revealed the dominance of Firmicutes and the presence of other bacterial groups in non-HTT-pretreated compost. Bacillus and Lysinibacillus were identified as the main contributors to food waste composting. HTT is advantageous for pretreating easily degradable food waste, leading to the production of compost with reduced phytotoxicity.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Environmental Sciences
Mitsuhiko Koyama, Ayami Kakiuchi, Fadhil Syukri, Tatsuki Toda, Quyen Ngoc Minh Tran, Kiyohiko Nakasaki
Summary: Recent research shows that inoculated Neurospora sp. can enhance organic matter decomposition and improve ammonia conversion in thermophilic composting of organic sludge. Pretreated sludge with Neurospora sp. exhibited significantly higher organic matter decomposition and improved ammonia conversion rate. Enzymes with high activity during pretreatment were identified to be involved in the degradation of hardly degradable fractions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Tosawat Loakasikarn, Yutaro Kubota, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: While seeding materials do not significantly affect the degradation of organic matter during composting, they do play a significant role in influencing microbial community succession. Enzyme gene abundance becomes similar across different seeding materials as composting progresses. Microorganisms present during composting may possess similar enzymatic potential despite being in different microbial communities.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Syazni Zainul Kamal, Mitsuhiko Koyama, Fadhil Syukri, Tatsuki Toda, Quyen Ngoc Minh Tran, Kiyohiko Nakasaki
Summary: The study aimed to identify enzymes that enhance sludge hydrolysis in a thermophilic composting system for ammonia recovery from shrimp pond sludge. Among the four hydrolytic enzyme classes assessed, proteases from Streptomyces griseus were the most effective at hydrolysing non-dissolved nitrogen in the sludge, increasing ammonia conversion efficiency in the process.
ENVIRONMENTAL RESEARCH
(2022)
Article
Agricultural Engineering
Masaaki Fujiwara, Mitsuhiko Koyama, Shinichi Akizuki, Syuhei Ban, Tatsuki Toda
Summary: This study found that the chemical composition and anaerobic digestibility of aquatic weeds differ from terrestrial plants. The amount and ratio of lignocellulosic components (cellulose, hemicellulose, and lignin) have significant effects on the anaerobic digestion process. While cellulose can be degraded and converted to methane, the presence of lignin and xylose limits the degradability of cellulose, thus regulating methane production from aquatic weeds.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Environmental Sciences
Jericho Victor Mercado, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: This study investigated the short-term changes in biochemical pathways and microbial contributions in anaerobic digestion under varying organic load shocks using a starch and high polypeptide mixture as substrate. Results showed that methane yield decreased with increasing organic load, microbial alpha diversity decreased, and specific microbial groups had varying contributions to different pathways with increasing organic load. The study provides insights into how anaerobic digesters respond to fluctuations in organic loading rate.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Jericho Victor Mercado, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: Fluctuations in the organic loading rate (OLR) of anaerobic digestion (AD) can lead to unstable methane production. This study investigated the impact of repeated high OLR shocks on the microbial community dynamics within AD. The results showed that less frequent organic load shocks led to a decrease in methane productivity and a breakdown of the microbiome network structure. The identification of influential species in the network structure revealed the importance of Anaerovorax, Spirochaetales, and Synergistales. It was inferred that the OLR shocks shifted the microbiome activity away from methane production pathways. Overall, developing a resilient AD microbiome would be necessary for small-scale AD systems that can withstand infrequent organic loading shocks.
Article
Engineering, Environmental
Thien-Phuc Nguyen, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: This study investigated the impact of oxygen supply rate (OSR) on composting of organic waste. The research revealed that lower OSR resulted in delayed degradation of organic matter and severe oxygen deficiency. It was also found that enzymes showed lower activity under deficient oxygen conditions. Additionally, specific microorganisms dominated during different stages of oxygen deficiency composting.
Article
Biotechnology & Applied Microbiology
Qilong Li, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: This study evaluated the effect of storage time on an enriched microbial community and found that inoculation with freshly enriched microorganisms and dry-stored microorganisms for 30 days showed higher levels of organic matter decomposition. The increase in the relative abundance of efficient decomposer microorganisms contributed to this effect. Additionally, the reuse of enriched microorganisms from long-term storage enhanced organic matter decomposition, indicating the potential of stored inocula in composting.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Green & Sustainable Science & Technology
Nunthaphan Vikromvarasiri, Mitsuhiko Koyama, Winarto Kurniawan, Nipon Pisutpaisal, Kiyohiko Nakasaki
Summary: The study found that intermittent substrate feeding can reduce the effects of intermediate volatile fatty acids, especially propionic acid and improve methane production. The microbial community changes suggested that Trichococcus is the key bacterium in glycerol degradation, while Delftia and Desulfuromonas may compete with methanogens. This study highlights the importance of microbial communities in methane production from glycerol.
Article
Environmental Sciences
Jericho Victor Mercado, Mitsuhiko Koyama, Kiyohiko Nakasaki
Summary: This study investigated the short-term response of the anaerobic digestion microbiome to organic load shocks, comparing the acclimatization to simple and complex substrates. The results showed that the microbiome acclimatized to a complex substrate maintained its methane productivity despite high organic load shocks, while the microbiome acclimatized to a simple substrate struggled to handle such shocks efficiently. The alpha-diversity of the microbiome decreased with an increase in organic load for the simple substrate acclimatization but remained unaffected for the complex substrate acclimatization, indicating that acclimatization to a complex substrate increased the robustness and flexibility of the microbiome towards organic load shocks.
ENVIRONMENTAL RESEARCH
(2023)
