Journal
JOURNAL OF BIOPHOTONICS
Volume 13, Issue 6, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201960126
Keywords
POCT; Raman reporters; rational design; SERS
Categories
Funding
- Deutsche Forschungsgemeinschaft [SCHL 594/12-1]
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The characteristic vibrational spectroscopic fingerprint of Raman reporter molecules adsorbed on noble metal nanoparticles is employed for the identification of target proteins by the corresponding surface-enhanced Raman scattering (SERS) nanotag-labeled antibodies. Here, we present the modular synthesis of thiolated polyenes with two to five C=C double bonds introduced via stepwise Wittig reactions. The experimental characterization of their electronic and vibrational properties is complemented by density functional theory calculations. Highly SERS-active nanotags are generated by using the thiolated polyenes as Raman reporter molecules in Au/Au core/satellite supraparticles with multiple hot spots. The cytokines IL-1 beta and IFN-gamma are detected in a duplex SERS-based lateral flow assay on a nitrocellulose test strip by Raman microscopy. The thiolated polyenes are suitable for use in immuno-SERS applications such as point-of-care testing as well as cellular and tissue imaging.
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