Article
Microbiology
Javier Moreno-Morales, Andrea Vergara, Tomislav Kostyanev, Jesus Rodriguez-Bano, Herman Goossens, Jordi Vila
Summary: The study evaluated the efficacy of a molecular diagnostic kit based on loop-mediated isothermal amplification technology in detecting the presence of carbapenemases in Acinetobacter spp. directly from BAL samples. The kit was able to rapidly identify carbapenemases in less than 30 minutes with a sensitivity of 10(3) CFU/ml, demonstrating significant advantages for clinical practice.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Lichao Fan, Bo Guan, Moxin Cheng, Chang Liu, Yao Tian, Ran Li, Yu Chen
Summary: In this study, we evaluated the performance of TB LAMP in diagnosing pediatric PTB. The results showed that TB-LAMP had higher sensitivity when used in combination with smear microscopy, and had higher specificity and sensitivity compared to other diagnostic methods. TB-LAMP using BALF samples provided faster results, allowing early and accurate diagnosis of PTB.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Matteo Boattini, Gabriele Bianco, Marco Iannaccone, Elisa Zanotto, Francesca Sidoti, Andre Almeida, Francesco Giuseppe De Rosa, Rossana Cavallo, Cristina Costa
Summary: The study evaluated the potential contribution of molecular assays in optimizing empirical antibiotic therapy for critically ill patients with pneumonia caused by carbapenemase- and/or CTX-M-producing Enterobacterales. Positive molecular data could have led to earlier optimization of antibiotic therapy in approximately 50% of cases.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Microbiology
Veronika Pilchova, Diana Seinige, Isabel Hennig-Pauka, Kathrin Buettner, Amir Abdulmawjood, Corinna Kehrenberg
Summary: The newly developed LAMP detection method for G. parasuis showed high specificity and sensitivity, with 100% analytical specificity and a detection limit of 10 fg/mu L. Additional validation with clinical samples and a colorimetric visual detection method without specialized equipment further demonstrated its effectiveness for field use.
Article
Infectious Diseases
Yo Sugawara, Hideharu Hagiya, Yukihiro Akeda, Dan Takeuchi, Noriko Sakamoto, Yuki Matsumoto, Daisuke Motooka, Isao Nishi, Kazunori Tomono, Shigeyuki Hamada
Summary: The study revealed the presence of carbapenemase-producing Enterobacterales (CPE) in healthy residents of Yangon, some of which were related to strains isolated from retail food and clinical settings in the area. This indicates the potential transmission of CPE from clinical and food sources to healthy individuals in the community.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Forough Babazadeh, Roghayeh Teimourpour, Mohsen Arzanlou, Hadi Peeridogaheh, Mehdi Yousefipour, Jafar MohammadShahi
Summary: The objective of this study was to evaluate the phenotypic and molecular characterization of ESBL/AmpC- and carbapenemase-producing K. pneumoniae isolates in Iran. The results showed that K. pneumoniae isolates had high resistance rates to cefazolin and cefotaxime, while meropenem and amikacin exhibited high sensitivity rates. The most frequent carbapenemase-encoding genes were bla(OXA-48) and bla(IMP), while bla(KPC) and bla(GTM) were not detected. Among the ESBL and AmpC beta-lactamase-encoding genes, bla(TEM) had the highest frequency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Wenjuan Feng, Siqiang Niu, Yanbin Chang, Xiaojiong Jia, Shifeng Huang, Ping Yang
Summary: This study demonstrated the use of colorimetric loop-mediated isothermal amplification (LAMP) for rapid and accurate detection of the five major carbapenemase gene families. The LAMP system showed higher sensitivity and retained the same specificity compared to PCR, making it a valuable tool for clinical diagnosis of CPE infections. Furthermore, the study showed concordant results between LAMP and PCR methods for clinical isolates, supporting the potential use of LAMP in routine clinical laboratories.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Laura Berneking, Lucia Asar, Anna Both, Benjamin Berinson, Martin Aepfelbacher, Marc Luetgehetmann, Holger Rohde
Summary: The Isoplex CRE-ART assay has been found to rapidly identify carbapenemase genes in CR-GNB, improving options for pathogen characterization in clinical microbiological and infection control diagnostics.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Review
Infectious Diseases
Oumar Ouchar Mahamat, Marie Kempf, Manon Lounnas, Abelsalam Tidjani, Mallorie Hide, Julio A. Benavides, Christian Carriere, Anne-Laure Banuls, Helene Jean-Pierre, Abdoul-Salam Ouedraogo, Yann Dumont, Sylvain Godreuil
Summary: The study investigated the knowledge gaps on ESBL-E and CPE in West and Central Africa using a 'One Health' approach, revealing major gaps in understanding ESBL and CPE in animals and the environment. The results emphasize the urgent need to improve active surveillance programs in each country and to support antimicrobial stewardship.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Infectious Diseases
Mihret Tilahun
Summary: Antibiotic resistance is a global issue, and this study assessed the prevalence of multidrug-resistant bacteria among surgical patients in northeast Ethiopia. The findings revealed a high prevalence of resistant strains and highlighted the need for enhanced infection prevention and control measures.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biophysics
Hyo Yong Kim, Jayeon Song, Hyun Gyu Park
Summary: An ultrasensitive isothermal method for detecting microRNA using target-induced chain amplification reaction (CAR) is described. The strategy involves a hairpin probe (HP) that opens upon binding to target miRNA, leading to exponential amplification reactions and the production of double-stranded DNA products that can be monitored through fluorescent signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Microbiology
Owen Higgins, Alexandra Chueiri, Louise O'Connor, Sinead Lahiff, Liam Burke, Dearbhaile Morris, Nicola Maria Pfeifer, Belen Gonzalez Santamarina, Christian Berens, Christian Menge, Manuela Canica, Vera Manageiro, Veljo Kisand, Marwa M. M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno Gonzalez-Zorn, Terry J. J. Smith
Summary: CTX-M ESBL-producing E. coli is an increasing public health concern, and accurate identification of CTX-M variants is crucial for disease monitoring and treatment. CTX-M-1 and CTX-M-15 are the main ESBL-producing Enterobacteriaceae associated with animal and human infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Tsuyoshi Sekizuka, Kentaro Itokawa, Rina Tanaka, Masanori Hashino, Koji Yatsu, Makoto Kuroda
Summary: Metagenomic analysis was conducted to evaluate the presence of antimicrobial resistance genes (ARGs) and heavy-metal resistance genes (HMRGs) in effluents from multiple urban wastewater treatment plants (WWTPs). The study revealed significant seasonal differences in the distribution of ARGs, and HMRGs were detected in all WWTPs.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Nathan D. Maulding, Spencer Seiler, Alexander Pearson, Nicholas Kreusser, Joshua M. Stuart
Summary: The SARS-CoV-2 pandemic has posed challenges in understanding the health effects caused by the novel infection. Transcriptomic RNA sequencing has provided new insights into viral mechanisms and host responses. The study reveals tissue specificity of SARS-CoV-2 expression, inferred lipopolysaccharide response, and co-regulation with CXCL, SPRR, and S100. These findings have implications for future therapeutic research.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Immunology
Jesus Rodriguez-Bano, Belen Gutierrez-Gutierrez
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Francesc Escrihuela-Vidal, Achim J. Kaasch, Maja Von Cube, Siegbert Rieg, Winfried V. Kern, Harald Seifert, Kyoung-Ho Song, Chun-Hsing Liao, Robert Tilley, Hannah Gott, Matt Scarborough, Claire Gordon, Martin J. Llewelyn, Richard Kuehl, Laura Morata, Alex Soriano, Jonathan Edgeworth, Enrique Ruiz De Gopegui, Emmanuel Nsutebu, Jose Miguel Cisneros, Vance G. Fowler, Guy Thwaites, Joaquin Lopez-Contreras, Gavin Barlow, Hugo Guillermo Ternavasio-De La Vega, Jesus Rodriguez-Bano, Luis Eduardo Lopez-Cortes
Summary: This study analysed the adherence and impact of quality-of-care indicators (QCIs) in the management of Staphylococcus aureus bloodstream infection. The results showed that adherence to QCIs was associated with reduced 90-day mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Alaa Atamna, Ili Margalit, Gida Ayada, Tanya Babich, Pontus Naucler, John Karlsson Valik, Christian G. Giske, Natividad Benito, Ruben Cardona, Alba Rivera, Celine Pulcini, Manal Abdel Fattah, Justine Haquin, Alasdair Macgowan, Bibiana Chazan, Anna Yanovskay, Ronen Ben Ami, Michal Landes, Lior Nesher, Adi Zaidman-Shimshovitz, Kate McCarthy, David L. Paterson, Evelina Tacconelli, Michael Buhl, Susanna Mauer, Jesus Rodriguez-Bano, Marina de Cueto, Antonio Oliver, Enrique Ruiz de Gopegui, Angela Cano, Isabel Machuca, Monica Gozalo-Marguello, Luis Martinez-Martinez, Eva M. Gonzalez-Barbera, Iris Gomez Alfaro, Miguel Salavert, Bojana Beovic, Andreja Saje, Manica Mueller-Premru, Leonardo Pagani, Virginie Vitrat, Diamantis Kofteridis, Maria Zacharioudaki, Sofia Maraki, Yulia Weissman, Mical Paul, Yaakov Dickstei, Dafna Yahav
Summary: This study aimed to evaluate outcomes of P. aeruginosa bacteremia among older adults (> 80 years) and found that disease severity, place of acquisition, and background conditions were associated with mortality, while antimicrobial regimen was not. Therefore, preventive efforts and early diagnosis might be beneficial for improving outcomes in older adults with P. aeruginosa bacteremia.
Letter
Infectious Diseases
Sonsoles Salto-Alejandre, Marta Carretero-Ledesma, Pedro Camacho-Martinez, Judith Berastegui-Cabrera, Carmen Infante, Regino Rodriguez-Alvarez, Jorge Alba, Patricia Perez-Palacios, Emilio Garcia-Diaz, Cristina Roca, Julia Praena, Maria Jose Blanco-Vidal, Sonia Santibanez, Rocio Valverde-Ortiz, Javier Nieto-Arana, Concepcion Garcia-Garcia, David Gutierrez-Campos, Natalia Maldonado, Gabriel Bernal, Miguel Angel Gomez-Bravo, Jose Manuel Sobrino, Manuela Aguilar-Guisado, Rocio Alvarez-Marin, Josune Goikoetxea-Aguirre, Jose Antonio Oteo, Zaira R. Palacios-Baena, Alvaro Pascual, Jose Antonio Lepe, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Jeronimo Pachon, Javier Sanchez-Cespedes, Elisa Cordero
JOURNAL OF INFECTION
(2023)
Letter
Infectious Diseases
Alexander Rombauts, Carmen Infante, Mikel del Alamo Martinez de Lagos, Jorge Alba, Adoracion Valiente, Carla Donado-Mazarron, Marta Carretero-Ledesma, Regino Rodriguez-Alvarez, Sonia Omatos, Zaira R. Palacios-Baena, Gabriela Abelenda-Alonso, Maria del Mar Silva-Sanchez, Ane Josune Goikoetxea-Agirre, Jose A. Oteo, Jesus Rodriguez-Bano, Elisa Cordero, Carlota Gudiol, Javier Sanchez-Cespedes, Jordi Carratala
JOURNAL OF INFECTION
(2023)
Article
Immunology
Joachim Doua, Jeroen Geurtsen, Jesus Rodriguez-Bano, Oliver A. Cornely, Oscar Go, Aina Gomila-Grange, Andrew Kirby, Peter Hermans, Andrea Gori, Valentina Zuccaro, Stefan Gravenstein, Marc Bonten, Jan Poolman, Michal Sarnecki
Summary: This multinational study describes the clinical profile of patients with invasive Escherichia coli disease (IED) in tertiary care hospitals. Most patients with IED were adults aged >= 60 years, and the urinary tract was the most common source of infection. Systemic inflammatory response syndrome (SIRS) was identified in 77.4% of patients, and the case-fatality rate was 20.0%. A proposed clinical case definition showed a 96.1% agreement with physician's diagnoses.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Nerea Castillo-Fernandez, Pedro Maria Martinez Perez-Crespo, Elena Salamanca-Rivera, Laura Herrera-Hidalgo, Aristides de Alarcon, Maria Dolores Navarro-Amuedo, Teresa Marrodan Ciordia, Maria Teresa Perez-Rodriguez, Juan Sevilla-Blanco, Alfredo Jover-Saenz, Jonathan Fernandez-Suarez, Carlos Arminanzas-Castillo, Jose Maria Reguera-Iglesias, Clara Natera Kindelan, Lucia Boix-Palop, Eva Leon Jimenez, Fatima Galan-Sanchez, Alfonso del Arco Jimenez, Alberto Bahamonde-Carrasco, David Vinuesa Garcia, Alejandro Smithson Amat, Jordi Cuquet Pedragosa, Isabel Maria Reche Molina, Ines Perez Camacho, Esperanza Merino de Lucas, Belen Gutierrez-Gutierrez, Jesus Rodriguez Bano, Luis Eduardo Lopez Cortes
Summary: A post-hoc analysis of two Spanish cohorts showed that sequential OPAT is as safe and effective as conventional hospitalization for treating patients with S. aureus bacteremia.
Review
Public, Environmental & Occupational Health
Usman Abubakar, Menier Al-Anazi, Zainab Alanazi, Jesus Rodriguez-Bano
Summary: The COVID-19 pandemic has led to changes in the rate of multidrug resistant gram positive and gram negative bacteria. Studies have shown an increase in MRSA and VRE infection/colonization rates, while ESBL E. coli and ESBL Klebsiella pneumoniae rates have decreased. Overall, there has been an increase in the rate of multidrug resistant CRE infection/colonization during the pandemic, while the rate of CRPA infection has decreased.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Medicine, General & Internal
Lucia Blasco, Inmaculada Lopez-Hernandez, Miguel Rodriguez-Fernandez, Javier Perez-Florido, Carlos S. Casimiro-Soriguer, Sarah Djebara, Maya Merabishvili, Jean-Paul Pirnay, Jesus Rodriguez-Bano, Maria Tomas, Luis Eduardo Lopez Cortes
Summary: This article describes a case study of a patient with a multidrug-resistant prosthetic vascular graft infection caused by Pseudomonas aeruginosa, which was successfully treated with a combination of phages (PT07, 14/01, and PNM) and ceftazidime-avibactam (CZA). After phage therapy, a new infection with a wild-type strain of P. aeruginosa occurred, but this strain was susceptible to β-lactams and quinolones. Microbiology and whole genome sequencing techniques were used to analyze the clinical strains. The clinical isolates before and after phage therapy showed a clonal relationship with important genomic changes that may have contributed to resistance to this therapy. Phenotypic studies revealed a decrease in the Minimum Inhibitory Concentration (MIC) to β-lactams and quinolones, as well as an increase in biofilm production and phage-resistant mutants in the clinical isolate of P. aeruginosa after phage therapy.
FRONTIERS IN MEDICINE
(2023)
Article
Infectious Diseases
Natalia Roson-Calero, Clara Balleste-Delpierre, Javier Fernandez, Jordi Vila
Summary: In recent decades, there has been an increasing trend in infections caused by multidrug-resistant (MDR) bacteria, which are associated with higher morbidity and mortality. Various interventions should be implemented to reduce the emergence and spread of MDR bacteria. This mini-review aims to compile and discuss different strategies for eradicating MDR bacteria from the gut microbiota.
Article
Medicine, General & Internal
Salvador Perez-Galera, Jose M. Bravo-Ferrer, Maria Paniagua, Tomislav Kostyanev, Marlieke E. A. de Kraker, Jan Feifel, Jesus Sojo-Dorado, Joost Schotsman, Rafael Canton, George L. Daikos, Biljana Carevic, Gorana Dragovac, Lionel K. Tan, Lul Raka, Adriana Hristea, Pierluigi Viale, Murat Akova, Jose Maria Reguera, Lucia Valiente de Santis, Julian Torre-Cisneros, Angela Cano, Emmanuel Roilides, Lili Radulovic, Cenk Kirakli, Evelyn Shaw, Matthew E. Falagas, Vicente Pintado, Herman Goossens, Marc J. Bonten, Belen Gutierrez-Gutierrez, Jesus Rodroguez-Bano
Summary: A study found that the main risk factors for CRE infections in hospitals with high incidence included previous colonization, urinary catheter use, and exposure to broad spectrum antibiotics.
Article
Health Care Sciences & Services
Nithya Babu Rajendran, Fabiana Arieti, Carla Alejandra Mena-Benitez, Liliana Galia, Maela Tebon, Julio Alvarez, Beryl Primrose Gladstone, Lucie Collineau, Giulia De Angelis, Raquel Duro, William Gaze, Siri Goepel, Souha S. Kanj, Annemarie Kaesbohrer, Direk Limmathurotsakul, Estibaliz Lopez de Abechuco, Elena Mazzolini, Nico T. Mutters, Maria Diletta Pezzani, Elisabeth Presterl, Hanna Renk, Jesus Rodriguez-Bano, Oana Sandulescu, Federico Scali, Robert Skov, Thirumalaisamy P. Velavan, Cuong Vuong, Evelina Tacconelli
Summary: Strategic and standardised approaches are crucial for analyzing and reporting surveillance data on antimicrobial resistance (AMR) and informing antibiotic policies. This paper describes a multidisciplinary initiative involving experts from the human, animal, and environmental sectors to develop proposals for structuring and reporting full-scale AMR and antimicrobial consumption/antimicrobial residue surveillance data. The recommendations from this study can support national and regional plans to reduce resistance rates and promote a One Health approach to antimicrobial policy.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Infectious Diseases
Sara Batista, Mariana Fernandez-Pittol, Lorena San Nicolas, Diego Martinez, Marc Rubio, Montserrat Garrigo, Jordi Vila, Griselda Tudo, Julian Gonzalez-Martin
Summary: Patients with chronic pulmonary diseases infected by Mycobacterium avium complex (MAC) often develop complications and suffer from treatment failure due to biofilm formation. There is a lack of correlation between in vitro susceptibility tests and the treatment of clinical isolates producing biofilm. This study found that the use of potential antibiofilm agents (PAAs) in combination with three-drug combinations can effectively reduce the bactericidal concentration in biofilm forms of MAC isolates.
Article
Critical Care Medicine
Marius Troseid, Jose R. Arribas, Lambert Assoumou, Aleksander Rygh Holten, Julien Poissy, Vida Terzic, Fulvia Mazzaferri, Jesus Rodriguez Bano, Joe Eustace, Maya Hites, Michael Joannidis, Jose-Artur Paiva, Jean Reuter, Isabel Puentmann, Thale D. J. H. Patrick-Brown, Elin Westerheim, Katerina Nezvalova-Henriksen, Lydie Beniguel, Tuva Borresdatter Dahl, Maude Bouscambert, Monika Halanova, Zoltan Peterfi, Sotirios Tsiodras, Michael Rezek, Matthias Briel, Serhat Unal, Martin Schlegel, Florence Ader, Karine Lacombe, Cecilie Delphin Amdal, Serge Rodrigues, Kristian Tonby, Alexandre Gaudet, Lars Heggelund, Joy Mootien, Asgeir Johannessen, Jannicke Horjen Moller, Beatriz Diaz Pollan, Anders Aune Tveita, Anders Benjamin Kildal, Jean-Christophe Richard, Olav Dalgard, Victoria Charlotte Simensen, Aliou Balde, Lucie de Gastines, Marta del Alamo, Burc Aydin, Fridtjof Lund-Johansen, Mary-Anne Trabaud, Alpha Diallo, Bente Halvorsen, John-Arne Rottingen, Evelina Tacconelli, Yazdan Yazdanpanah, Inge C. Olsen, Dominique Costagliola
Summary: The study conducted a placebo-controlled trial of baricitinib in severe/critical COVID cases. The results showed that baricitinib did not reduce mortality within 60 days, and there may be safety risks in vaccinated participants, which require further investigation.
Article
Urology & Nephrology
Marina Almenara-Tejederas, Maria A. Rodriguez-Perez, Maria J. Moyano-Franco, Marina De Cueto-Lopez, Jesus Rodriguez-Bano, Mercedes Salgueira-Lazo
Summary: This retrospective study analyzed 406 tunneled catheters implanted in 325 patients over a period of 14 years to evaluate the incidence of tunneled catheter-related bacteremia and identify potential factors associated with the first episode of bacteremia. The results showed a low incidence rate of bacteremia, which had minimal impact on catheter survival. Staphylococcus epidermidis was the most frequently isolated microorganism, and the Palindrome(R) catheter, jugular vein, and being the first vascular access were identified as significant protective factors against tunneled catheter-related bacteremia.
JOURNAL OF NEPHROLOGY
(2023)
