Article
Biochemistry & Molecular Biology
Huili Sun, Xiaoyun Cao, Xinyue Wang, Wei Zhang, Wenxu Li, Xiaoqian Wang, Siqi Liu, Deguo Lyu
Summary: Exogenous melatonin promotes anthocyanin accumulation in pears by activating the RBOH-mediated ROS signaling pathway. PuRBOHF plays a crucial role in anthocyanin biosynthesis and is involved in a positive feedback loop.
Article
Plant Sciences
Jianlong Liu, Zhiwei Deng, Hongwei Sun, Jiankun Song, Dingli Li, Shaoling Zhang, Ran Wang
Summary: The study investigated the mechanism underlying the red coloration of pear skin using the 'Xinqihong' cultivar as an experimental model. Transcriptome sequencing identified genes related to light, calcium, hormone signaling, and anthocyanin biosynthesis that contribute to the color differences in red pears. These findings provide valuable insights for improving fruit quality and commercial value.
Article
Plant Sciences
Weiting Liu, Yun Wei, Shoufeng Sha, Yaxiu Xu, Hongjian Li, Hui Yuan, Aide Wang
Summary: In this study, it was demonstrated that histone acetylation plays a role in promoting anthocyanin accumulation in pears through the regulation of key genes. The color of horticultural plants is an important trait, and the anthocyanin content directly affects the nutritional and commercial value of the fruits. Therefore, developing new varieties with bright colors is important for fruit breeding.
PLANT CELL REPORTS
(2023)
Article
Agronomy
Yunting Zhang, Shanlin Li, Xianjie Gu, Diya Lei, Bing Zhao, Honglan Tang, Yuanxiu Lin, Yan Wang, Mengyao Li, Qing Chen, Ya Luo, Xiaorong Wang, Haoru Tang, Yong Zhang
Summary: Red-skinned pear is a promising commercial fruit due to its attractive appearance and nutritious value, with anthocyanin being the determinant of its red coloration. Differences in anthocyanin accumulation exist among red pear cultivars with different genetic backgrounds, and the expression profiles of critical anthocyanin biosynthetic genes show a tendency towards decreased anthocyanin content during fruit development.
Article
Agriculture, Multidisciplinary
Sun Hui-li, Wang Xin-yue, Shang Ye, Wang Xiao-qian, Du Guo-dong, Lu De-guo
Summary: The application of preharvest melatonin significantly influenced the color development of Nanhong pears, enhancing the concentrations of anthocyanins and flavonols while reducing hydroxycinnamate and flavanol concentrations. The transcription levels of most anthocyanin biosynthetic genes and anthocyanin-related transcription factors were induced by melatonin treatment. Moreover, melatonin application stimulated the expression of melatonin biosynthesis-related genes, leading to an increase in endogenous melatonin concentration and providing insights into melatonin-induced fruit coloration for potential agricultural applications.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Horticulture
Yujie Liu, Haowei Cao, Zhixia Zhao, Yao Xu, Xieyu Li, Lijuan Xiao, Rui Zhai, Chengquan Yang, Lingfei Xu, Zhigang Wang
Summary: The red color of the pear peel in red-skinned European pear is due to the accumulation of anthocyanins. PbZAT12, a zinc finger protein gene, was found to regulate anthocyanin biosynthesis independent of PbMYB10. PbZAT12 positively influenced anthocyanin accumulation in 'Red Zaosu' pears and upregulated the expression levels of PbDFR, PbANS, and PbUFGT. It directly bound to the promoters of these genes and activated their transcriptional activity.
Article
Plant Sciences
Junbei Ni, Apekshika T. Premathilake, Yuhao Gao, Wenjie Yu, Ruiyan Tao, Yuanwen Teng, Songling Bai
Summary: Ethylene induces anthocyanin biosynthesis in most fruits, but inhibits it in pears. The transcription factor PpERF105 activated the expression of PpMYB140, which then inhibited the anthocyanin biosynthesis in pears. The competition between PpMYB140 and PpMYB114 led to the formation of a complex that further inhibited anthocyanin biosynthesis.
Article
Plant Sciences
Lu Zhang, Ruiyan Tao, Simai Wang, Yuhao Gao, Lu Wang, Shulin Yang, Xiao Zhang, Wenjie Yu, Xinyue Wu, Kunfeng Li, Junbei Ni, Yuanwen Teng, Songling Bai
Summary: This study revealed that PpZAT5 is an upstream negative regulator of PpBBX18, which decreases anthocyanin content in pear by inhibiting the expression of PpBBX18. Moreover, the expression of PpZAT5 shows an opposite pattern to that of anthocyanin-related structural genes induced by light.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xieyu Li, Fangxin Xiang, Wei Han, Bingqing Qie, Rui Zhai, Chengquan Yang, Zhigang Wang, Lingfei Xu
Summary: This study identified the function of PbbHLH2 containing the MIR-domain in regulating anthocyanin biosynthesis in Zaosu pear peel. The MIR-domain was shown to interact with R2R3-MYB TFs and activate the expression of late biosynthetic genes, ultimately increasing the anthocyanin content. These findings suggest a key role of the MIR domain in the interaction between MIR-domain bHLH and R2R3-MYB TFs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Horticulture
Lorenzo Bergonzoni, Sara Alessandri, Cecilia Domenichini, Luca Dondini, Giuseppina Caracciolo, Marco Pietrella, Gianluca Baruzzi, Stefano Tartarini
Summary: This paper reports the molecular analysis of 33 red-fleshed pear accessions using 18 SSR markers. The study identified cases of synonymy and homonymy, and found 6 unique genotypes among the samples. S-genotypes were established to further understand the genetic relationships among these landraces.
SCIENTIA HORTICULTURAE
(2023)
Article
Agronomy
Wantian Yao, Diya Lei, Xuan Zhou, Haiyan Wang, Jiayu Lu, Yuanxiu Lin, Yunting Zhang, Yan Wang, Wen He, Mengyao Li, Qing Chen, Ya Luo, Xiaorong Wang, Haoru Tang, Yong Zhang
Summary: This study investigates the accumulation of anthocyanins in red pears by subjecting red pear flesh callus to varying sugar sources, MS concentrations, light qualities, and temperatures. The findings reveal that sucrose induces anthocyanin accumulation and increases related metabolites and genes as sugar and MS concentrations increase. The use of red-blue light and a temperature of 15 degrees C further enhances the production of anthocyanins and secondary metabolites, as well as upregulates the synthesis of anthocyanin-related genes.
Article
Biochemistry & Molecular Biology
Yan Zhou, Muhammad Ali Mumtaz, Yonghao Zhang, Huangying Shu, Yuanyuan Hao, Xu Lu, Shanhan Cheng, Guopeng Zhu, Zhiwei Wang
Summary: This study identified key genes involved in anthocyanin synthesis through experiments with purple pepper fruit under different light conditions, providing a reference for further research on the regulatory mechanism of anthocyanin biosynthesis in peppers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Yifan Xing, Wenjing Sun, Yuying Sun, Jialin Li, Jie Zhang, Ting Wu, Tingting Song, Yuncong Yao, Ji Tian
Summary: This study investigated the dynamics of apple fruit phosphoproteome in response to light using high-performance liquid chromatography-tandem mass spectrometry analysis. The findings revealed that mitogen-activated protein kinase MdMPK6 directly interacted with and activated transcription factor MdHY5 through phosphorylation under light conditions, thereby promoting anthocyanin accumulation in apple fruit.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Meiyi Yang, Junxing Song, Xu Zhang, Ruitao Lu, Azheng Wang, Rui Zhai, Zhigang Wang, Chengquan Yang, Lingfei Xu
Summary: This study identified PbWRKY26 as a key gene involved in the regulation of malic acid accumulation in pear. The expression of PbWRKY26 was positively correlated with malic acid content in different pear cultivars. Additionally, PbWRKY26 was found to regulate a malate dehydrogenase gene, PbMDH3. This research provides valuable insights into the metabolic regulatory network associated with malate accumulation in pears.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Horticulture
Xiaonan Dai, Qingyu Li, Fudong Jiang, Zhizhong Song, Xiaoli Tang, Shuqi Su, Ruotong Yao, Haiying Yang, Yanqing Yang, Hongxia Zhang, Jianzhao Li
Summary: This study clarified the mechanism of anthocyanin accumulation in "Red Bartlett" pear through transcriptome analysis, and identified the genes and transcription factors associated with anthocyanin biosynthesis and regulation.
SCIENTIA HORTICULTURAE
(2022)