Article
Biochemistry & Molecular Biology
Ling Chen, Yuan Lv, Feifei Xu, Fang Zhong
Summary: This study evaluated the effects of collagen peptides enriched with X-Hyp or X-Hyp-Gly on skin properties and found that oral supplementation with these peptides significantly improved skin elasticity and collagen synthesis, suggesting their potential for enhancing skin health.
Article
Chemistry, Applied
Yu Iwasaki, Mizuho Nakatogawa, Ayaka Shimizu, Yoshio Sato, Yasutaka Shigemura
Summary: This study demonstrated that gelatin ingestion led to significant increases in prolyl-hydroxyproline and hydroxyprolyl-glycine levels in the plasma. Low molecular weight gelatin hydrolysate was more effective in enhancing the concentrations of these peptides.
Article
Food Science & Technology
Jihyun Won, Juhyung Kang, Keumhan Noh, Hee-Chul Chung, Wonku Kang
Summary: This study represents the first individual pharmacokinetics of Gly-Pro-Hyp, Pro-Hyp, and Gly-Pro in vivo. Gly-Pro-Hyp and Pro-Hyp showed a double-peak phenomenon after oral administration, while Gly-Pro had low levels in systemic circulation. Gly-Pro-Hyp tended to exhibit higher first-pass metabolism than Pro-Hyp.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yoshifumi Kimira, Takahiro Sato, Mayu Sakamoto, Yoshihiro Osawa, Hiroshi Mano
Summary: This study investigates the effects of Pro-Hyp on the differentiation of ATDC5 chondrogenic cells under hypoxic conditions. The results show that Pro-Hyp significantly promotes the early differentiation of chondrocytes under physiological hypoxic conditions, suggesting its potential role as a remodeling factor or extracellular matrix remodeling signal that regulates chondrocyte differentiation in hypoxic cartilage.
Article
Biochemistry & Molecular Biology
Anjeeta Rani, Anamika Sindhu, Tzu-Jou Yao, Jia-Cherng Horng, Pannuru Venkatesu
Summary: Collagen mimetic peptides (CMPs) have promising applications in tissue engineering, regenerative medicine, and biomaterials. This study explores the self-assembly of small CMPs to form large-scale collagen structures, and demonstrates the use of choline chloride (ChCl) to manipulate the stability and aggregation of CMP triple helices. These findings contribute to the understanding of factors responsible for triple helix stability and have potential implications for the discovery of new biomaterials.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yoshifumi Kimira, Konosuke Osawa, Yoshihiro Osawa, Hiroshi Mano
Summary: Glucocorticoids can induce muscle atrophy, but this study found that Prolyl-hydroxyproline (Pro-Hyp) has potential therapeutic effects on dexamethasone-induced muscle atrophy.
Article
Biochemistry & Molecular Biology
Seiko Koizumi, Yoko Okada, Shiroh Miura, Yuuki Imai, Keiji Igase, Yasumasa Ohyagi, Michiya Igase
Summary: In this study, the effects of collagen peptides (CP) containing high concentrations of prolyl-hydroxyproline and hydroxyprolyl-glycine on advanced glycation end products (AGEs) levels in the skin and subcutaneous blood vessel walls were investigated. A total of 31 individuals aged 47-87 years were randomly assigned to receive either CP or a placebo for 12 weeks. The CP group showed significantly lower AGEs levels and a slightly lower insulin resistance index compared to the placebo group, suggesting that fish-derived CP may be effective in reducing AGEs levels and improving insulin resistance.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Takaki Tominaga, Jiapeng Huang, Shuo Wang, Miwa Noguchi, Yishan Tong, Momoko Asano-Oritani, Katsuhiko Suzuki
Summary: Several food components, including polyphenols and phytonutrients, have immunomodulatory effects. Collagen, known for its various bioactivities, can promote wound healing and relieve symptoms of bone/joint disease. This study investigated the immunomodulatory effects of collagen-derived dipeptides and amino acids on macrophages and peripheral blood mononuclear cells (PBMC), and found that there is no difference in cytokine secretion between dipeptides and amino acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Qi Tian, Shi-Ming Li, Bo Li
Summary: This study identified eleven peptides in the absorbates of fish skin collagen hydrolysates, with OGSA showing the strongest inhibitory effect on platelet aggregation and thrombus formation. Molecular docking results suggested that OG-containing peptides may target the P2Y12 receptor. The abundant PG(OG) sequence in the collagen amino acid sequence of H. Molitrix indicates potential for developing the hydrolysates as dietary supplements for preventing cardiovascular diseases.
Article
Fisheries
Pedro L. Castro, Sergio Plasencia, Maria Jesus Zamorano, Luis Guerrero, Anna Claret, Jose A. Beltran, Juan Calanche, Rafael Gines
Summary: The study demonstrated that supplementation of 1.2% or 2% crystalline L-Hyp in high plant-protein diets positively affected the growth performance of adult European sea bass and increased total collagen deposition in muscle.
AQUACULTURE REPORTS
(2021)
Article
Multidisciplinary Sciences
Shiro Jimi, Seiko Koizumi, Kenji Sato, Motoyasu Miyazaki, Arman Saparov
Summary: The study showed that Pro-Hyp can accelerate muscle tissue regeneration and reduce scars formation. Experimental results indicated that Pro-Hyp is beneficial for wound healing, reducing fibrosis, and promoting muscle regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Soo-Il Kim, Sin-Hye Park, Woojin Na, Yong Chul Shin, Moon-Sik Oh, Young Eun Sim, Yulong Zheng, Ae Hyang Kim, Il-Jun Kang, Young-Hee Kang
Summary: Pangasius hypophthalmus fish skin collagen hydrolysates (fsCH) can retard postmenopausal osteoporosis by inhibiting bone loss and enhancing bone density.
Article
Dentistry, Oral Surgery & Medicine
Cristiane Mayumi Inagati, Debora Lopes Salles Scheffel, Giovana Anovazzi, Juliana Rosa Luiz Alonso, Marcelly Tupan Christoffoli, David Henry Pashley, Carlos Alberto De Souza Costa, Josimeri Hebling
Summary: This study compared the proteolytic activity and degradation rate of human and bovine dentin matrices, finding differences in some aspects but no statistical difference after long-term incubation.
JOURNAL OF APPLIED ORAL SCIENCE
(2021)
Article
Chemistry, Organic
Wei-Ming Wang, Chen-Hsu Yu, Jing-Yuan Chang, Ting-Hsuan Chen, Yan-Chen Chen, Yi-Ting Sun, Szu-Huan Wang, Shu-Chuan Jao, Richard P. Cheng
Summary: The study monitored the reversible thermal folding and unfolding of heterotrimeric collagen triple helices with varying number of Pro-Hyp-Gly triplets, showing that increasing the number of triplets leads to higher T-m and Delta G(unfold). Inserting a Pro-Hyp-Gly triplet increased Delta G(unfold) by 2.0 +/- 0.2 kcal mol(-1). Moreover, the T-m difference between the most stable and second most stable combinations decreased with increasing number of Pro-Hyp-Gly triplets.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ji Eun Lee, Yong Chool Boo
Summary: The study introduces a new strategy utilizing glycianamide and ascorbic acid (AA) to enhance collagen production in cells, leading to improved wound healing capabilities.
Article
Food Science & Technology
Tomoko T. Asai, Hitoshi Takamura, Sumi Sugiyama
Summary: This study investigated the mechanism by which the addition of rapeseed oil (RO) or milk fat (MF) affects gel formation in hen eggs. The results showed that most RO droplets were released from the liquid egg before heating but became trapped after heating, while MF globules remained trapped both before and after heating. Differential scanning calorimetry and transmission electron microscopy revealed that RO droplets were trapped in a void protein matrix due to enhanced protein denaturation, while MF globules suppressed protein denaturation and were covered with a fine matrix. Therefore, the addition of fat or oil to liquid egg affects the interactions between proteins and lipids, as well as the degree of protein denaturation during heating, promoting the generation of specific textures in egg-based gels.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)