Article
Medicine, General & Internal
Farah Omran, Alice M. Murphy, Awais Z. Younis, Ioannis Kyrou, Jana Vrbikova, Vojtech Hainer, Petra Sramkova, Martin Fried, Graham Ball, Gyanendra Tripathi, Sudhesh Kumar, Philip G. McTernan, Mark Christian
Summary: This study investigated the impact of endotoxin on mitochondrial function and browning capacity in human adipocytes. The results showed that endotoxin directly contributes to impaired mitochondrial function and reduced browning capacity of adipocytes, exacerbating metabolic consequences. Bariatric surgery, which reduces endotoxin levels, is associated with improved adipocyte functionality.
Article
Chemistry, Medicinal
Minji Choi, Sulagna Mukherjee, Jong Won Yun
Summary: Trigonelline, a major alkaloid component of fenugreek, has potential as an anti-obesity compound by inducing browning of white adipocytes and improving lipid metabolism through regulation of gene and protein expression, as well as mitochondrial biogenesis pathways.
PHYTOTHERAPY RESEARCH
(2021)
Article
Medicine, Research & Experimental
Trang Thi Huyen Dang, Jong Won Yun
Summary: Our study revealed a previously unknown mechanism in the regulation of browning by CYP2E1 in 3T3-L1 white adipocytes, suggesting that CYP2E1 is a promising molecular target for the treatment of obesity and its related diseases. It was found that deficiency of CYP2E1 results in reduced adipogenesis and lipogenesis as well as induction of brown adipocyte-like phenotype. Moreover, CYP2E1 inhibition deters the activation of the 83-adrenergic receptor and its downstream targets, highlighting its potential role in lipid metabolism and browning in white adipocytes.
Article
Pharmacology & Pharmacy
Trang Thi Huyen Dang, Minji Choi, Huong Giang Pham, Jong Won Yun
Summary: Studies have shown that CYP2F2 can negatively regulate lipid metabolism and browning in adipocytes by inhibiting the activation of β-3 adrenergic receptor and its downstream regulators. Inhibitors of CYP2F2 could potentially be used as drugs for treating obesity by enhancing energy expenditure.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuna Kim, Hyanggi Ji, Dehun Ryu, Eunae Cho, Deokhoon Park, Eunsun Jung
Summary: This study investigated the effects of Albizia julibrissin Leaf extracts (AJLE) on adipocytes. AJLE inhibited adipogenesis by reducing the expression of PPARγ and C/EBPs, which regulate fat synthesis and storage. It also induced the differentiation of brown adipocytes and increased mitochondrial biogenesis by increasing the expression of brown adipocyte markers and mitochondrial genes. AJLE's ability to inhibit adipogenesis and induce brown adipocyte differentiation may have therapeutic potential in obesity and related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuelei Zhao, Xuexin Li, Ye Tian, Jindi Zhao, Wensai Yu, Lifan Zhang, Wei Wei, Jie Chen
Summary: This study found that nonivamide can induce browning of porcine adipocytes, inhibit adipogenesis and promote lipolysis, as well as up-regulate thermogenesis gene markers and mitochondrial biogenesis-related genes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Francesca Cuomo, Carmela Dell'Aversana, Teresa Chioccarelli, Veronica Porreca, Francesco Manfrevola, Chiara Papulino, Vincenzo Carafa, Rosaria Benedetti, Lucia Altucci, Gilda Cobellis, Gilda Cobellis
Summary: Maintaining energy balance is crucial for human health. This study highlights the role of Hif3 alpha in the transformation of white adipocytes to brown adipocytes, which activates beneficial thermogenesis and rewires energy metabolism. These findings have important implications for combating obesity and type 2 diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Nutrition & Dietetics
Vittoria Schirinzi, Carolina Poli, Chiara Berteotti, Alessandro Leone
Summary: The increasing prevalence of overweight and obesity indicates that current strategies are insufficient to tackle this epidemic. Brown adipose tissue (BAT) is receiving attention as a potential weapon against obesity. Research is focused on developing strategies to increase BAT activity and energy expenditure. This review summarizes knowledge on molecules that promote white-to-brown adipose tissue conversion and assesses the potential role of thermogenic nutraceuticals in combating obesity.
Article
Biochemistry & Molecular Biology
Subramani Manigandan, Sulagna Mukherjee, Jong Won Yun
Summary: The study identified FAM107A as a factor that interferes with fat browning in white adipocytes. Deficiency of FAM107A promoted mitochondrial biogenesis and significantly upregulated core fat-browning marker proteins, as well as beige-specific genes. The data revealed a previously unknown mechanism of FAM107A in the regulation of lipid metabolism and its significant role in metabolic homeostasis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Food Science & Technology
In-Seon Bae, Jeong Ah Lee, Soo-Hyun Cho, Hyoun-Wook Kim, Yunseok Kim, Kangmin Seo, Hyun-Woo Cho, Min Young Lee, Ju Lan Chun, Ki Hyun Kim
Summary: This study demonstrates for the first time that rabbit meat extract induces browning of white adipocytes through activation of the AMPK pathway, leading to a reduction in adipocyte differentiation and triglyceride content, and an increase in hormone-sensitive lipase activity.
Article
Food Science & Technology
Siru Chen, Yankun Fu, Tianlin Wang, Zhenglin Chen, Peijun Zhao, Xianqing Huang, Mingwu Qiao, Tiange Li, Lianjun Song
Summary: This study found that 2'-fucosyllactose (2'-FL) can promote the browning of white adipose tissue, reduce lipid accumulation, and increase mitochondrial density and the proportion of UCP1-positive cells.
Article
Food Science & Technology
Se-Jun Park, Dong-Hyun Shon, Yang-Hwan Ryu, Yong Ko
Summary: Herbal extracts from Ephedra sinica show dual effects on adipocytes, inhibiting adipogenesis in immature cells and promoting browning in mature cells.
Article
Biochemistry & Molecular Biology
Manigandan Subramani, Jong Won Yun
Summary: The study reveals that deficiency of LCP1 induces browning of white adipocytes by promoting mitochondrial biogenesis and enhancing expressions of key brown fat-specific genes and proteins. Additionally, loss of LCP1 promotes lipid catabolism, suppresses adipogenesis, and ameliorates cellular stress in adipocytes. The mechanism involves activation of beta 3-AR and the ERK signaling pathway independently.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Food Science & Technology
Huong Giang Pham, Trang Thi Huyen Dang, Jong Won Yun
Summary: Salvianolic acid B (Sal B) promotes white fat browning, suppresses lipogenesis, and enhances lipolysis in white adipocytes, suggesting its beneficial effects in preventing and treating obesity.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biochemistry & Molecular Biology
Subramani Manigandan, Jong Won Yun
Summary: This study reveals the role of CYFIP2 in adipocyte browning, showing that its deficiency promotes fat browning and mitochondrial biogenesis. Additionally, CYFIP2 deficiency promotes lipid catabolism and suppresses adipogenesis, lipogenesis, and autophagy. The study also uncovers the underlying mechanism by which CYFIP2 induces browning in white adipocytes, involving the activation of mTORC1 and suppression of the GABA-BR signaling pathway.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Cell Biology
Andrea Irazoki, Marta Martinez-Vicente, Pilar Aparicio, Cecilia Aris, Esmaeil Alibakhshi, Maria Rubio-Valera, Juan Castellanos, Luis Lores, Manuel Palacin, Anna Guma, Antonio Zorzano, David Sebastian
Summary: Sarcopenia is a major factor contributing to disability in aged individuals, with chronic inflammation playing a role in its development. The key unresolved question is the factors driving inflammation during aging and participating in sarcopenia development. Mitochondrial dysfunction and alterations in mitophagy induce inflammatory responses, but whether accumulation of damaged mitochondria in muscle triggers inflammation during aging is still unknown.
Article
Physiology
Cristina Martinez, Jessica Latorre, Francisco Ortega, Maria Arnoriaga-Rodriguez, Aina Lluch, Nuria Oliveras-Canellas, Francisco Diaz-Saez, Julian Aragones, Marta Camps, Anna Guma, Wifredo Ricart, Jose Manuel Fernandez-Real, Jose Maria Moreno-Navarrete
Summary: This study investigated the association between circulating NRG4 and insulin sensitivity in humans. The results showed a negative correlation between serum NRG4 and insulin sensitivity, and a positive correlation with the inflammatory marker hsCRP. In vitro experiments also revealed that NRG4 had an impact on hepatocyte metabolism. However, further confirmation is needed in human primary hepatocytes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jessica Latorre, Cristina Martinez, Francisco Ortega, Nuria Oliveras-Canellas, Francisco Diaz-Saez, Julian Aragones, Marta Camps, Anna Guma, Wifredo Ricart, Jose Manuel Fernandez-Real, Jose Maria Moreno-Navarrete
Summary: This study investigates the potential role of EGFR, ErbBs receptors and neuregulins in human adipose tissue physiology in obesity. The results show the association between adipose tissue ERBB2 and obesity, the relevance of EGFR on human adipogenesis, and suggest a possible adipogenic role of NRG2.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Andrea Irazoki, Isabel Gordaliza-Alaguero, Emma Frank, Nikolaos Nikiforos Giakoumakis, Jordi Seco, Manuel Palacin, Anna Guma, Lykke Sylow, David Sebastian, Antonio Zorzano
Summary: Some forms of mitochondrial dysfunction induce sterile inflammation through mitochondrial DNA recognition by intracellular DNA sensors. Opposite mitochondrial morphologies induce distinct inflammatory signatures, caused by differential activation of DNA sensors TLR9 or cGAS. Skeletal muscle mitochondrial fragmentation promotes TLR9-dependent inflammation, muscle atrophy, reduced physical performance and enhanced IL6 response to exercise, which improved upon chronic anti-inflammatory treatment.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ines Paccetti-Alves, Marta S. P. Batista, Catarina Pimpao, Bruno L. Victor, Graca Soveral
Summary: The natural polyphenolic compound Rottlerin (RoT) has shown potential as an anticancer agent by inhibiting target molecules implicated in tumorigenesis. It has been found to inhibit human AQP3 activity with an IC50 in the micromolar range. Molecular docking and simulations have revealed the structural determinants of RoT that explain its ability to inhibit AQP3. This multidisciplinary approach provides new information for aquaporin research and future drug design.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Monika Mlinaric, Ivan Lucic, Lidija Milkovic, Ines V. da Silva, Ivana Tartaro Bujak, Vesna Musani, Graca Soveral, Ana Cipak Gasparovic
Summary: Aquaporin 3 (AQP3) is a membrane protein that transports hydrogen peroxide, water, and glycerol. It is implicated in tumor progression and malignancy in breast cancer and may be a target for therapy. This study examined the relationship between AQP3 expression, disruption of lipid raft harboring EGFR, and the EGFR/PI3K/Akt signaling pathway in breast cancer cell lines. The results indicate that AQP3 may not directly affect the PI3K/Akt pathway but rather its role is cell-line specific.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ines V. da Silva, Diogo Coelho, Jose Antonio Mestre Prates, Graca Soveral, Paula A. Lopes
Summary: This study investigated the potential effect of a dietary amino acid mixture on LPS-challenged weaned piglets and found that the amino acid mixture may contribute to immune recovery. Further analysis revealed tissue-specific variation in the gene expression of AQPs and cytokines, distinguishing the small intestine and kidney from the liver and spleen.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Genetics & Heredity
Paula A. Lopes, Elisabete Fonseca, Ines V. da Silva, Emanuel Vigia, Jorge Paulino, Graca Soveral
Summary: This study analyzed the expression levels of AQP genes in pancreatic cancer tissues using RT-qPCR and found that the coordinated gene expression of AQPs was disrupted in tumor tissues.
Article
Biochemistry & Molecular Biology
Gema Fruhbeck, Leire Mendez-Gimenez, Sara Becerril, Beatriz Ramirez, Ana Wenting Hernandez-Pardos, Javier A. Cienfuegos, Victor Valenti, Rafael Moncada, Victoria Catalan, Javier Gomez-Ambrosi, Ines V. da Silva, Graca Soveral, Amaia Rodriguez
Summary: We investigated the role of AQP7 in the process of BAT whitening and found that its expression is upregulated in diet-induced obesity (DIO). Interestingly, the expression of AQP7 is downregulated after cold exposure or bariatric surgery, which is associated with the improvement of BAT whitening. Furthermore, AQP7 expression is regulated by specific lipogenic signals and correlated with the expression of lipogenic genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
J. Pimenta, I. V. da Silva, A. Casini, G. Soveral
Meeting Abstract
Biochemistry & Molecular Biology
C. Pimp, I. V. da Silva, I. Paccetti-Alves, A. F. Mosca, A. Casini, G. Soveral
Meeting Abstract
Biochemistry & Molecular Biology
P. M. Silva, I. V. da Silva, M. J. Sarmento, F. A. Carvalho, G. Soveral, N. C. Santos
Meeting Abstract
Biochemistry & Molecular Biology
I. V. da Silva, P. M. Silva, N. C. Santos, G. Soveral
Meeting Abstract
Peripheral Vascular Disease
Ines Vieira Da Silva, Ana Carolina Santos, Andreia Matos, Joana Ferreira, Alda Pereira Da Silva, Graca Soveral, Maria Clara Bicho, Irene Rebelo, Manuel Bicho
JOURNAL OF HYPERTENSION
(2022)