Article
Chemistry, Multidisciplinary
Lorena Wilson, Andres Illanes, Carminna Ottone, Oscar Romero
Summary: The production of lactofructose syrup from lactose through enzymatic conversion is a prime example of the circular economy, showcasing the possibilities of co-immobilized multi-enzyme systems. Understanding the key aspects of enzyme co-immobilization and the operating conditions is essential for developing successful biotransformation technologies.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2022)
Article
Chemistry, Analytical
Cynthia Nagy, Ruben Szabo, Attila Gaspar
Summary: This review provides an overview of the current trends in the development and application of microfluidic immobilized enzyme reactor (mu-IMER) technology in proteomic analysis. It focuses on microfabrication and enzyme immobilization techniques and discusses the potential future prospects based on the advantages and limitations of different approaches.
Article
Electrochemistry
Wassim El Housseini, Francois Lapicque, Steve Pontvianne, Neus Vila, Ievgen Mazurenko, Alain Walcarius, Mathieu Etienne
Summary: The electrochemical regeneration of reduced form of NADH was achieved in a hybrid flow reactor. The stable immobilization of all catalysts, including a rhodium catalyst, was carefully optimized. The system showed high activity and stability, allowing for efficient biosynthesis reactions with low cofactor concentration.
Article
Biochemistry & Molecular Biology
Carlos Murguiondo, Anna Mestre, Juan A. Mendez-Liter, Manuel Nieto-Dominguez, Laura de Eugenio, Maria Molina-Gutierrez, Maria Jesus Martinez, Alicia Prieto
Summary: The recombinant beta-xylosidase (rBxTW1) and its mutant, immobilized as magnetic cross-linked enzyme aggregates (mCLEAs), maintained or improved their catalytic properties, including the synthesis of various xylosides. The mCLEAs showed recyclability and improved thermal and pH stability, making them suitable for multiple cycles of synthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Wilhelm R. Glomm, Sileshi G. Wubshet, Diana Lindberg, Katinka R. Dankel, Nils K. Afseth, Per M. Stenstad, Heidi Johnsen
Summary: Immobilizing enzymes onto magnetic particles enables enzyme reusability and cost reduction in processes like enzymatic protein hydrolysis. The immobilized protease on magnetic silica particles showed high reusability and storage stability, performing well in hydrolysis of chicken meat and turkey tendons.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Huanhuan Sun, Huasheng Wang, Hongfeng Zhan, Chao Fan, Zuwen Liu, Hai Yan, Yu Pan
Summary: The study analyzed the major biological characteristics of the microcystinase (MlrA) enzyme by utilizing the amino acid sequence information from Sphingopyxis sp. USTB-05. Results showed that MlrA has a highly conserved domain and functions best as a neutral protease with good stability under specific temperature and pH conditions. Metal ions like Cu2+ and Zn2+ were found to deactivate MlrA, offering insights for future research on MC biodegradation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Biotechnology & Applied Microbiology
Siti Hajar Hamdan, Jonathan Maiangwa, Mohd Shukuri Mohamad Ali, Yahaya M. Normi, Suriana Sabri, Thean Chor Leow
Summary: Thermal stability is crucial in the search for novel lipases, with thermophilic microorganisms offering new opportunities for biotechnological applications. Enzyme structure modifications are essential for optimizing stability in thermophilic conditions, particularly in the food industry and biodiesel production. There is a trade-off between enzyme use in organic solvents and product generation, but enhancing stability is key for maintaining enzymatic activity regardless of the environment.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Nadya Dencheva, Sandra Oliveira, Joana Braz, Dariya Getya, Marc Malfois, Zlatan Denchev, Ivan Gitsov
Summary: This study demonstrates the successful non-covalent immobilization of Trametes versicolor laccase on neat and magnetically responsive polyamide 6 microparticles for catechol oxidation. The immobilized laccase exhibited higher activity than the native enzyme, with high adsorption efficiency and reusability.
Article
Biophysics
Ke Wang, Rui Lv, Shiyong Sun, Faqin Dong, Mingxue Liu, Jin Liu, Xiaoqin Nie
Summary: This study demonstrates a novel approach to immobilize enzymes on nanostructured substrates, constructing nanobiocatalysts for enhanced enzymatic performance. The immobilized alpha-amylase showed higher catalytic activity and stability over a wide range of pH values and temperatures, likely due to allosteric modulation. The study lays the foundation for further industrial applications utilizing the phenomenon of allosteric activation of enzymes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Food Science & Technology
Ibukunoluwa Fola Olawuyi, Jong Jin Park, Gwang Deok Park, Won Young Lee
Summary: In this study, the relationship between the molecular structure of Okra pectins (OKPs) and their emulsifying properties was investigated. Enzymatic treatment was used to modify the structure of OKP, and it was found that the presence of neutral sugar side-chains is crucial for long-term emulsion stability. This study provides valuable information for the extraction of OKP with good emulsification performance, which can be used as a natural emulsifier.
Article
Food Science & Technology
Chunli Kong, Caiping Duan, Shunzhi Zhang, Rui Liu, Yuanlin Sun, Sumei Zhou
Summary: This study co-modified black wheat bran (BWB) through extrusion and enzyme treatment, resulting in increased content of soluble dietary fiber (SDF) and improved physicochemical properties and nutritive functions. The co-modified BWB exhibited higher water holding capacity, oil holding capacity, and cholesterol adsorption capacity, as well as a looser and more porous microstructure. In vitro fermentation experiments showed that the co-modified BWB had a higher content of beneficial bacteria and promoted the production of butyric acid, indicating its high potential as a prebiotic. These findings are important for developing high-fiber cereal products.
Article
Chemistry, Physical
Helena Hronska, Vladimir Stefuca, Ema Ondrejkova, Maria Blahova, Jozef Visnovsky, Michal Rosenberg
Summary: A new method combining chemical synthesis and enzymatic hydrolysis was developed to prepare glycoside substrates with high selectivity. The immobilization of Hex in polyvinyl alcohol hydrogel capsules showed improved stability and retained high activity.
Article
Engineering, Chemical
Kaven Nguyen, Ion Iliuta, Francis Bougie, Louis-Cesar Pasquier, Maria C. Iliuta
Summary: A novel enzymatic CO2 capture process was evaluated and found to significantly reduce energy consumption, capital cost, and operating expenses compared to the benchmark MEA process. This technology is green, energy-efficient, non-toxic, and low-cost, showing promise for industrial applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Hannaneh Rasouli, Ion Iliuta, Francis Bougie, Alain Garnier, Maria C. Iliuta
Summary: A hybrid enzymatic CO2 absorption process using immobilized enzymes on a membrane and dispersed in a liquid phase was proposed. This process can achieve high absorption rates even if one component of the enzymatic system does not work properly.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ruchi Sahu, Anil K. Meghavarnam, Savitha Janakiraman
Summary: The immobilized Rhodococcus rhodochrous (RS-6) cells in agar showed the most effective conversion of acrylonitrile, achieving complete conversion in 120 minutes, with enhanced tolerance for both the substrate and product, and 50% retention of conversion ability after seven cycles of reuse, making it economical for large-scale production of acrylamide.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)