Article
Zoology
Yongwen Lin, Jinhua Huang, Komivi S. Akutse
Summary: The study demonstrates that plant volatiles can enhance the virulence of Lecanicillium lecanii by regulating chitinase activity. This provides important methods for enhancing the virulence of L. lecanii.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Microbiology
Yan Chen, Ning Zhou, Xueman Chen, Guoguang Wei, Alei Zhang, Kequan Chen, Pingkai Ouyang
Summary: In this study, a gene encoding beta-N-acetylglucosaminidase, designated NAGaseA, was cloned and functionally expressed in Escherichia coli. NAGaseA showed both transglycosylation and hydrolytic activities, making it a potential candidate for GlcNAc production.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Yanjun Zhang, Xiao Zhang, Weiliang Qiu
Summary: Lecanicillium lecanii (Zimmermann) Zare & Gams is used as an effective biopesticide for controlling sap-sucking insect pests on agricultural crops. However, its low fungicide tolerance limits its large-scale field application. In this study, a composite mutagenesis system was established to improve the propamocarb tolerance in L. lecanii. The mutagenesis efficiency was assessed by analyzing the changes in mutants in various aspects. The obtained mutant showed the highest ability to resist or avoid the damaging effects of propamocarb, indicating its potential for field application.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lidia Blaszczyk, Agnieszka Waskiewicz, Karolina Gromadzka, Katarzyna Mikolajczak, Jerzy Chelkowski
Summary: This study investigated the communities of Sarocladium and Lecanicillium found in maize seeds collected from fields in Poland, revealing their potential to produce selected bioactive substances. The results showed the ability of these fungi to synthesize specific compounds, as well as the need to reconsider the approach to studying the biological role of fungi inhabiting maize seeds.
Article
Entomology
Mariappan Sankara Rama Subramaniam, Azariah Babu, Bhabesh Deka
Summary: In this study, Lecanicillium lecanii was isolated from tea ecosystem and formulated into a wettable powder for controlling tea thrips, showing significant efficacy. Addition of jaggery under suitable conditions enhanced the efficacy of the biopesticide. The powder formulation was found to be safe for natural enemies in the tea ecosystem.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Nisha Sogan, Smriti Kala, Neera Kapoor, B. N. Nagpal, Ayyagari Ramlal, Aparna Nautiyal
Summary: Mosquitoes are carriers of various diseases and pose a major health concern. The use of synthetic insecticides for vector control has led to environmental and health issues. In this study, a bioactive compound from the entomopathogenic fungus LL was developed into granules which showed high toxicity against malaria-causing mosquitoes. The developed formulation was stable and had lethal effects on the mosquitoes, as supported by SEM and histopathology analysis.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Agronomy
Yusuf Ali Abdulle, Talha Nazir, Samy Sayed, Samy F. Mahmoud, Muhammad Zeeshan Majeed, Hafiz Muhammad Usman Aslam, Zubair Iqbal, Muhammad Shahid Nisar, Azhar Uddin Keerio, Habib Ali, Dewen Qiu
Summary: Whiteflies, polyphagous pests of solanaceous crops worldwide, can be controlled through the use of a partially purified protein extracted from the entomopathogenic fungus Lecanicillium lecanii. This protein showed a significant impact on reducing the fecundity rate of Bemisia tabaci, and upregulated defense pathways in cotton plants. Further research on the purification and characterization of this protein is necessary to explore its potential as a biological control method against B. tabaci and other related pests.
Article
Chemistry, Applied
Jing Li, Kunpeng Gao, Francesco Secundo, Xiangzhao Mao
Summary: Chitin is converted to GlcNAc by chitinases, essential for food and pharmaceutical industries. Two novel beta-N-acetylglucosaminidases were cloned and characterized, enabling the one-pot production of pure GlcNAc from chitin through enzymatic reactions.
Article
Biochemistry & Molecular Biology
Qifan Wu, Suwei Dong, Weimin Xuan
Summary: This article briefly introduces the methods for generating N-glycosylated proteins and peptides through endoglycosidase-catalyzed reactions and highlights the current challenges and opportunities in the scientific community.
Article
Biochemistry & Molecular Biology
Mireia Castejon-Vilatersana, Magda Faijes, Antoni Planas
Summary: This study focused on mutational strategies in the donor subsites of lacto-N-biosidase to reduce hydrolytic activity and enable transglycosylation for the synthesis of LNT. Mutants with residual hydrolase activity between 0.05% to 1.6% of the wild-type enzyme showed transglycosylating enzymes with LNT yields ranging from 10-30%. Mutations in Trp394 had a significant impact on the transglycosylation/hydrolysis ratio, with the W394F mutant demonstrating the highest biocatalytic efficiency in producing LNT at a yield of 32%.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xiao Zhang, Chong Ou, Huiying Liu, Sunaina Kiran Prabhu, Chao Li, Qiang Yang, Lai-Xi Wang
Summary: The study introduced a versatile and robust chemoenzymatic method for one-pot site-specific functionalization of antibodies, utilizing selectively modified disaccharide oxazoline derivatives as donor substrates. The method enabled direct labeling and functionalization of antibodies without the need for intermediates and enzyme separation, showing potential for applications in antibody-drug conjugation and beyond.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Burcu Pekdemir, Hatice Duman, Aysenur Arslan, Merve Kaplan, Melda Karyelioglu, Tolgahan ozer, Haci Mehmet Kayili, Bekir Salih, Bethany M. Henrick, Rebbeca M. Duar, Sercan Karav
Summary: Conjugated N-glycans are bioactive prebiotic compounds that selectively stimulate beneficial microbes. This study investigates the immobilization methods of the EndoBI-1 enzyme to develop its optimal application in food processes. The immobilization methods do not affect the enzyme specificity and result in the same glycan profile, allowing for full glycan release. These findings suggest the potential for future scale-up and commercialization of EndoBI-1.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Ye-Ming Zhou, Jun-Rui Zhi, Jiao-Jiao Qu, Xiao Zou
Summary: This study improved the understanding of the internal phylogeny of Lecanicillium by estimating its divergence times. The results showed that Lecanicillium has eight independent clades with different divergence times. Additionally, a new spider-pathogenic fungus species was described.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Huan Liu, Zengwei Liang, Yu Wang, Yingze Li, Ya Wang, Xin Guo, Wanyi Guan, Wei Zou, Zhigang Wu
Summary: A method to prepare long-acting GLP-1 through glycosylation has been developed, resulting in increased in vitro half-life and enhanced glucose-stabilizing capability. Selection of the appropriate modification site is crucial for improving the therapeutic properties of the modified peptides.
Article
Biochemistry & Molecular Biology
Yihao Liu, Junwen Ma, Ran Shi, Ting Li, Qiaojuan Yan, Zhengqiang Jiang, Shaoqing Yang
Summary: A beta-N-acetylhexosaminidase gene (CmHex187) from Catenibacterium mitsuokai was successfully expressed in Escherichia coli, showing strong transglycosylation activity with a high conversion ratio in the synthesis of an important HMO component, lacto-N-triose II (LNT2). The enzyme displayed optimal pH and temperature, promoting the growth of tested Lactobacillus and Bifidobacteria strains, making it a promising candidate for LNT2 production.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Danelia Ramirez-Ramirez, Monica L. Salgado-Lucio, Ana L. Roa-Espitia, Reyna Fierro, Humberto Gonzalez-Marquez, Joaquin Cordero-Martinez, Enrique O. Hernandez-Gonzalez
JOURNAL OF CELLULAR BIOCHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Fernando Gonzalez Chavez, Hugo Najera, Marco Antonio Leyva, Omar Solorza-Feria, Federico Gonzalez, Hiram Isaac Beltran
CRYSTAL GROWTH & DESIGN
(2020)
Article
Biochemistry & Molecular Biology
Jose Benito Lopez-Salguero, Reyna Fierro, Jean-Claude Michalski, Irma Jimenez-Morales, Tony Lefebvre, Oscar Mondragon-Payne, Steffi F. Baldini, Anne-Sophie Vercoutter-Edouart, Humberto Gonzalez-Marquez
GLYCOCONJUGATE JOURNAL
(2020)
Article
Veterinary Sciences
Ernesto Rodriguez-Tobon, Reyna Fierro, Humberto Gonzalez-Marquez, Francisco A. Garcia-Vazquez, Edith Arenas-Rios
Summary: The use of GSH as an antioxidant during sperm capacitation in boars improves sperm capacitation rate and in vitro fertilization success rate, but does not significantly impact embryo development.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Ivan Oseguera-Lopez, Serafin Perez-Cerezales, Paola Berenice Ortiz-Sanchez, Oscar Mondragon-Payne, Raul Sanchez-Sanchez, Irma Jimenez-Morales, Reyna Fierro, Humberto Gonzalez-Marquez
Article
Toxicology
Mayra Pamela Becerra-Amezcua, Monica Alejandra Rincon-Guevara, Irma Hernandez-Calderas, Xochitl Guzman-Garcia, Isabel Guerrero-Legarreta, Humberto Gonzalez-Marquez
Summary: Proteomic techniques were used to identify the most abundant proteins in the venom of Chrysaora chesapeakei from the Gulf of Mexico estuaries, revealing main toxins including metalloproteinase-zinc disintegrin and astacin-type metalloprotease. These components could be responsible for the main effects of this jellyfish venom. Additionally, nonpoisonous metalloproteinases were found in the connective tissue of the tentacles.
Article
Biochemistry & Molecular Biology
Leslye Samano-Hernandez, Reyna Fierro, Aude Marchal, Jean-Louis Gueant, Humberto Gonzalez-Marquez, Rosa-Maria Gueant-Rodriguez
Summary: NAFLD is a common chronic liver disease, and the study indicates that sitagliptin treatment is not effective in preventing NAFLD development, and may even cause adverse effects in the MCD rat model.
Article
Biochemistry & Molecular Biology
Ricardo Ceron, Mariana Peimbert, Arturo Rojo-Dominguez, Hugo Najera
Summary: This study reveals that lysozyme can form amyloid fibers under specific conditions, and the presence of copper ions inhibits their formation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Miguel Martinez-Jardines, Rosa O. Gonzalez-Robles, H. Gonzalez-Marquez, Anne-Claire Texier, Flor de Maria Cuervo-Lopez
Summary: The metabolic improvement and reduction of the inhibitory effect of 2-chlorophenol (2-CP) on the nitrification process occurring in nitrifying SBR systems can be associated with changes in the microbial community. The stable, complete, and efficient nitrifying process achieved in both SBRs could be related to the higher bacterial community richness and to the regular maintenance of ammonium and nitrite oxidizing bacteria tolerant to 2-CP along experimentation. Total 2-CP consumption might be associated with ammonium-oxidizing and/or heterotrophic bacteria related to Nitrosospira, Luteimonas, and Cohnella.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Paola Berenice Ortiz-Sanchez, Ana L. Roa-Espitia, Reyna Fierro, Aidee S. Lopez-Torres, Irma Jimenez-Morales, Ivan Oseguera-Lopez, Enrique O. Hernandez-Gonzalez, Humberto Gonzalez-Marquez
Summary: This study examines the effects of PFOS and PFOA on boar spermatozoa functionality and changes in the plasma membrane during capacitation. The results indicate that both substances reduce the capacitation ability of spermatozoa and impede the progesterone-induced acrosomal reaction. PFOS and PFOA also cause changes in the plasma membrane, including deregulation of membrane potential and alteration of membrane microdomain organization.
REPRODUCTIVE TOXICOLOGY
(2022)
Article
Food Science & Technology
Fernando Lazcano-Perez, Karina Bermeo, Hector Castro, Zayil Salazar Campos, Isabel Arenas, Ariana Zavala-Moreno, Sheila Narayan Chavez-Villela, Irma Jimenez, Roberto Arreguin-Espinosa, Reyna Fierro, Humberto Gonzalez-Marquez, David E. Garcia, Judith Sanchez-Rodriguez
Summary: Sea anemone venom, specifically the low molecular weight fraction, was found to impair the motility and capacitation of boar sperm cells and inhibit the HVA calcium current in rat chromaffin cells. These findings have implications for the pharmacological characterization of cnidarian venoms and toxins on voltage-gated calcium channels.
Article
Endocrinology & Metabolism
Lorena Ruiz-Valderrama, Jaqueline Posadas-Rodriguez, Herlinda Bonilla-Jaime, Maria del Rosario Tarrago-Castellanos, Humberto Gonzalez-Marquez, Isabel Arrieta-Cruz, Leticia Gonzalez-Nunez, Arturo Salame-Mendez, Ahiezer Rodriguez-Tobon, Jose Guadalupe Morales-Mendez, Edith Arenas-Rios
Summary: Obesity is associated with male infertility due to increased oxidative stress on sperm. This study found that overweight and obesity led to lower testosterone levels and poor sperm quality, although fertility was not greatly affected. However, the increased activities of CAT and GPX enzymes seemed to protect sperm from oxidative stress by counteracting the increase in ROS.
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Anahi Guadalupe Figueroa-Torres, Lisneth Osiris Matias-Aguilar, Erika Coria-Ramirez, Edmundo Bonilla-Gonzalez, Humberto Gonzalez-Marquez, Isabel Ibarra-Gonzalez, Jose Rubicel Hernandez-Lopez, Jesus Hernandez-Juarez, Victor Manuel Dominguez-Reyes, Irma Isordia-Salas, Abraham Majluf-Cruz
SAGE OPEN MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
