Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 145, Issue -, Pages 53-63Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.12.171
Keywords
O-phosphorylation; N-phosphorylation; NMR spectroscopy; Cell signaling process
Funding
- National Natural Science Foundation of China [21778042, 41876072, 91856126]
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Protein phosphorylation is a major switch mechanism for cell signaling process regulation within eukaryotic cells. Abnormal phosphorylation is either a cause or consequence of human diseases. It is imperative to comprehensively delve into the relationship between phosphorylation events and cell signaling transduction. NMR spectroscopy, a potent tool for monitoring protein phosphorylation events, is applicable for identifying the phospho-sites, quantifying the kinetic rate, discovering kinase/phosphatase inhibitors and delineating phosphorylation crosstalk with other post-translational modifications. Here, we decipher the recent progress in the investigation of eukaryotic protein O-phosphorylation by NMR spectroscopy. We focus specifically on the dynamic establishment of O-phosphorylation and its role in the cell signaling processes. Simultaneously, we positively propose a strategy for the investigation of acid-labile and high-energy N-phosphorylation by NMR spectroscopy. We expect that the strategy could enrich the investigation of protein N-phosphorylation. (C) 2019 Published by Elsevier B.V.
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