Article
Medicine, Research & Experimental
Aichun Li, Qin Yang, Guohua Lou, Yanning Liu, Hongguang Xia, Zhi Chen
Summary: In this study, F-nec was identified as a novel potent RIP1 inhibitor that efficiently blocked TNF-alpha-induced necroptosis in human and mice cells. Pre-treatment with F-nec prevented hepatic necrosis and attenuated LPS/GalN-induced acute liver failure by suppressing JNK-triggered inflammation, highlighting its potential for use in the treatment of inflammatory liver diseases driven by RIP1.
Article
Cell Biology
Yingjie Liu, Jun Peng, Xiaochuan Xiong, Liang Cheng, Xiaobing Cheng
Summary: This study found that Tofacitinib inhibits the proliferation and inflammation of rheumatoid arthritis synovial fibroblasts through modulating the STAT6/miR-425-5p/IGF1 signal axis, providing a novel therapeutic strategy for improving rheumatoid arthritis.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Cell Biology
Michele Patricia Rode, Adny Henrique Silva, Julia Cisilotto, Daiane Rosolen, Tania Beatriz Creczynski-Pasa
Summary: This study evaluated exosomal miRNAs derived from metastatic prostate cancer cell lines LNCaP and PC-3, identifying 14 candidates for prognostic biomarkers. miR-425-5p was associated with residual tumor, pathological staging, and TP53 status in prostate cancer samples.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Xiaoyan Dou, Xiaoliang Yu, Shujing Du, Yu Han, Liang Li, Haoran Zhang, Ying Yao, Yayun Du, Xinhui Wang, Jingjing Li, Tao Yang, Wei Zhang, Chengkui Yang, Feng Ma, Sudan He
Summary: MiR-324-5p negatively regulates necroptosis by targeting MLKL expression, and its downregulation by interferons facilitates the activation of necroptosis for host antiviral defense.
Review
Chemistry, Medicinal
Philip A. Harris
Summary: RIP1 kinase inhibitors are a new field of interest for treating diseases in the inflammatory, neurodegenerative, and oncology areas, as evidenced by the large amount of patent activity. Despite the widespread interest, only a few of these inhibitors have progressed to clinical trials, and further research is needed to determine the optimal clinical use of RIP1 inhibition.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2021)
Article
Biochemistry & Molecular Biology
Yuguo Yi, Weigao Zhang, Liang Tao, Qianchao Shao, Qian Xu, Yuxin Chen, Haibing Zhang, Jianfa Zhang, Dan Weng
Summary: This study investigated the role of RIP1 kinase in APAP-induced acute liver injury through genetic or pharmacological inhibition, providing evidence that RIP1 kinase activity plays an important role in the pathogenesis of APAP-induced liver injury. The results demonstrated that RIP1 kinase inactivation significantly attenuated APAP-induced liver injury and mortality, and that Nec-1, a RIP1 kinase inhibitor formulated with PEG400, could efficiently alleviate hepatotoxicity induced by APAP.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sara Dominguez, Eugene Varfolomeev, Robert Brendza, Kim Stark, Joy Tea, Jose Imperio, Hai Ngu, Timothy Earr, Oded Foreman, Joshua D. Webster, Amy Easton, Domagoj Vucic, Baris Bingol
Summary: Genetic inactivation of RIP1 kinase activity did not benefit disease progression in SOD1-Tg mice, while inhibition of kinase activity may provide benefit in acute injury models.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Medicine, Research & Experimental
Tingting Xu, Mengyan Xie, Xinming Jing, Huning Jiang, Xi Wu, Xinzhu Wang, Yongqian Shu
Summary: In this study, bioinformatic analysis and experimental validation revealed that miR-26b-5p was downregulated in Helicobacter pylori-infected gastric cancer (GC) cells and GC tissues. Low expression of miR-26b-5p promoted GC proliferation in vitro and in vivo and was associated with poor prognosis in GC patients. Mechanistically, miR-26b-5p directly targeted PDE4B and CDK8, leading to decreased phosphorylation and nuclear translocation of STAT3, which regulated GC proliferation. Importantly, miR-26b-5p was transcriptionally suppressed by STAT3, forming a miR-26b-5p-PDE4B/CDK8-STAT3 positive feedback loop. This newly identified feedback loop may serve as a promising therapeutic target for inflammation-related GC progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Reproductive Biology
Yinling Zhao, Donglan Yuan, Dandan Zhu, Tianhui Xu, Aihua Huang, Li Jiang, Chiwen Liu, Hua Qian, Xinhua Bu
Summary: In this study, it was found that MSC-AS1 and miR-425-5p were aberrantly expressed in ovarian cancer, with MSC-AS1 inhibiting ovarian cancer progression by modulating the levels of miR-425-5p. These findings may provide a promising therapeutic target for the treatment of ovarian cancer.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Cell Biology
Sara R. Oliveira, Pedro A. Dionisio, Maria M. Gaspar, Leonor Correia Guedes, Miguel Coelho, Mario M. Rosa, Joaquim J. Ferreira, Joana D. Amaral, Cecilia M. P. Rodrigues
Summary: The study reveals a protective role of miR-335 in experimental models of Parkinson's disease, showing its ability to combat inflammation and neurodegenerative events.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xin Chen, Rongfeng Zhu, Jinjin Zhong, Yongfa Ying, Wenxin Wang, Yating Cao, Hanyi Cai, Xiang Li, Jianwei Shuai, Jiahuai Han
Summary: Researchers used super-resolution microscopy to study the nanoscale structure of cellular necrosomes and discovered rod-shaped mosaic structures formed by RIP1 and RIP3 oligomers. They also revealed the roles and regulatory mechanisms of RIP1 and RIP3 in cell death.
NATURE CELL BIOLOGY
(2022)
Review
Cell Biology
Giovanni Luca Beretta, Nadia Zaffaroni
Summary: Necroptosis is a cell death mechanism characterized by inflammatory reactions and is involved in viral defense, inflammatory diseases, and drug response of tumors such as prostate cancer.
Article
Cell Biology
Lan Gao, Zhongjie Zhai, Qindong Shi, Jinqi Yan, Linjing Zhou, Yongxin Wu, Qinjing Zeng, Gang Tian, Hao Li
Summary: MiR-501-5p expression is down-regulated in septic patients with myocardial dysfunction. Overexpression of miR-501-5p improves cardiac function, reduces myocardial injury, and attenuates apoptosis and inflammation. MiR-501-5p exerts its effects by inhibiting the binding of NR4A3 and Bcl-2.
Article
Biochemistry & Molecular Biology
Cristina Morsiani, Salvatore Collura, Federica Sevini, Erika Ciurca, Valentina Rosa Bertuzzo, Claudio Franceschi, Gian Luca Grazi, Matteo Cescon, Miriam Capri
Summary: The study aimed to evaluate the changes in circulating microRNA (c-miR) in the blood of liver transplant recipients before and after transplantation, and to correlate their levels with gold standard biomarkers and outcomes such as rejection or complications. The results showed that c-miR changes were associated with follow-up time, and miR-122-5p, miR-92a-3p, and miR-18a-5p exhibited the same trend after transplantation, with an increase in their levels in patients with complications. Compared to changes in standard haemato-biochemical parameters, the importance of c-miRs as potential noninvasive biomarkers for monitoring patient outcomes was confirmed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Huiling Zhou, Lijun Liu, Xiaolong Ma, Jian Wang, Jinfu Yang, Xinmin Zhou, Yifeng Yang, Haidan Liu
Summary: Vinblastine, a first-line anti-tumor drug, has been associated with myocardial damage, the underlying molecular mechanism of which remains unknown. This study demonstrates that necroptosis is involved in vinblastine-induced myocardial damage, and targeting necroptosis could potentially alleviate chemotherapy-related myocardial injury.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)