Journal
FOOD CONTROL
Volume 106, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2019.106713
Keywords
Evaluation; ELISA; Veterinary antibiotics; Detection; Milk
Categories
Funding
- Special Fund for Agroscientific Research in the Public Interest [201403071]
- Project of Risk Assessment on Raw Milk [GJFP2019008]
- Agricultural Science and Technology Innovation Program [ASTIP-IAS12]
- Modern Agro-Industry Technology Research System of China [CARS-36]
- Scientific Research Project for Major Achievements of The Agricultural Science and Technology Innovation Program (ASTIP) [CAAS-ZDXT2019004]
- University of Liege-Gembloux Agro-Bio Tech
- AgriculturelsLife
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Antibiotics are increasingly found in dairy products. To ensure consumer safety, rapid screening methods which are accurate and cost-effective are highly in demand. A multiplexed detection technology was tested for quantification of these four kinds of antibiotics simultaneously in milk. The visual microarray technology based on enzyme-linked immunosorbent assays was utilized in this method, which can detect multiple target molecules simultaneously. This colorimetric technology provides direct results that can be detected using common commercial microplate scanners. The limit of detection (LOD) were calculated as 3.30, 3.39, 2.42 and 4.88 mu g kg(-1) for quinolones, tetracycline, lincomycin and streptomycin, respectively. The limits of quantity (LOQ) were defined as 8.97, 8.43, 5.36 and 10.97 mu g kg(-1) for quinolones, tetracycline, lincomycin and streptomycin, respectively. The accuracy of method was also adequate for these four substances with the recovery rates ranging from 77.6% to 116.4% were obtained for the detection of practical samples. According to our evaluation, this methodology can be used for rapid screening of antibiotics in internal quality control programs in dairy industry.
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