Article
Medicine, General & Internal
Stephen K. Brannan, Sharon Sawchak, Andrew C. Miller, Jeffrey A. Lieberman, Steven M. Paul, Alan Breier
Summary: The study showed that combined use of xanomeline and trospium in patients with schizophrenia led to a significant improvement in symptoms but was associated with cholinergic and anticholinergic adverse events, highlighting the need for larger and longer trials to determine efficacy and safety.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Multidisciplinary Sciences
Ana C. Laly, Kristina Sliogeryte, Oscar J. Pundel, Rosie Ross, Michael C. Keeling, Deepa Avisetti, Ahmad Waseem, Nuria Gavara, John T. Connelly
Summary: The keratin cytoskeleton plays a critical role in sensing matrix rigidity, with keratinocytes adapting to increased stiffness by forming a rigid interconnected network and boosting cell stiffness. Mutations disrupting keratin stability hinder the normal mechanical response and impair mechanotransduction. Depletion of cytolinker proteins, however, enhances mechanoresponsiveness and up-regulates lamin A/C.
Review
Cell Biology
Gerhard Wiche
Summary: This essay explores the role of plectin and its various isoforms in mediating intermediate filament network functions. It discusses how plectin acts as an IF recruiter and its global role as a modulator of IF functionality. The essay also presents a working model that describes a unifying mechanism underlying how plectin-IF networks control actomyosin-generated forces and contribute to mechanotransduction.
Article
Multidisciplinary Sciences
Anna Schepers, Charlotta Lorenz, Peter Nietmann, Andreas Janshoff, Stefan Klumpp, Sarah Koster
Summary: The mechanical properties of cells are largely determined by the cytoskeleton, with the intermediate filament network being the most extensible and stress-resilient. A multiscale approach is used to analyze the contributions of single-filament mechanics, filament length, and interactions between filaments to vimentin IF network mechanics. Hydrophobic contributions primarily affect filament elongation kinetics, while electrostatics influence filament-filament interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Bhuvanasundar Renganathan, James P. Zewe, Yuan Cheng, Jean-Michel Paumier, Mark Kittisopikul, Karen M. Ridge, Puneet Opal, Vladimir I. Gelfand
Summary: Gigaxonin acts as an adaptor protein for E3 ubiquitin ligase and is essential for ubiquitination and degradation of intermediate filament (IF) proteins. Mutations in the GAN gene, which encodes gigaxonin, cause giant axonal neuropathy characterized by abnormal accumulation of IFs. This study shows that loss of gigaxonin inhibits IF transport along microtubules by the motor protein kinesin-1, leading to abnormal IF distribution.
Article
Neurosciences
Laura McMartin, Marianna Kiraly, H. Craig Heller, Daniel V. Madison, Norman F. Ruby
Summary: The study found that loss of circadian timing leads to dentate granule cells maintaining a more depolarized resting membrane potential and higher levels of tonic inhibition, which may explain the spatial memory deficits observed in circadian-arrhythmic hamsters. Increased inhibition in response to muscarinic receptor activation could attenuate excitation in dentate-CA3 microcircuits.
Article
Pharmacology & Pharmacy
Banu Aydin, Mehmet Zafer Goren, Zehra Kanli, Hulya Cabadak
Summary: In this study, the mechanism of action of beta-adrenergic receptors agonists and antagonists on apoptosis in chronic myeloid leukaemia cells was investigated. The combined treatment of beta-adrenergic and cholinergic drugs was found to decrease cell proliferation in K562 cells by inducing the mitochondrial-dependent intrinsic apoptosis pathway.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Afrooz Dabbaghizadeh, Alexandre Pare, Zacharie Cheng-Boivin, Robin Dagher, Sandra Minotti, Marie-Josee Dicaire, Bernard Brais, Jason C. Young, Heather D. Durham, Benoit J. Gentil
Summary: Autosomal Recessive Spastic Ataxia of the Charlevoix Saguenay (ARSACS) is a genetic disease caused by mutation in the SACS gene, resulting in loss of function of the protein sacsin. The SacsJ domain of sacsin plays a key role in regulating the neurofilament (NF) and vimentin intermediate filament (IF) networks in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anton S. Shakhov, Irina B. Alieva
Summary: The endothelium plays a key role in the transcytosis of lipoproteins. Vimentin, as a main protein of endothelial intermediate filaments, is involved in cell polarization, migration, and control over endothelial cell functions. Vimentin also serves as an indicator of cardiovascular system state and participates in the development of atherosclerosis.
Article
Computer Science, Interdisciplinary Applications
Muhammad Safeer, Mohamed A. Khadimallah, Muhammad Tai, Muzamal Hussain, Elimame Elaloui, Abdelouahed Tounsi
Summary: The study discovered protein structures that form intermediate filaments through computerized analysis of the amino acid sequence of human epidermal keratin. It found that the nonlocal parameter has a significant impact on the dynamics of intermediate filaments, influencing their buckling behavior under different conditions and strengths of the nonlocal parameter.
COMPUTERS AND CONCRETE
(2021)
Article
Biochemistry & Molecular Biology
Dina Aweida, Shenhav Cohen
Summary: Maintenance of desmin intermediate filaments is crucial for muscle function. ATAD1, a AAA-ATPase, plays a key role in facilitating disassembly and degradation of ubiquitinated desmin, preventing muscle loss and atrophy. ATAD1 interacts with PLAA and UBXN4 to enhance its function.
Article
Multidisciplinary Sciences
Xiaoming Zhou, Yi Lin, Masato Kato, Eiichiro Mori, Glen Liszczak, Lillian Sutherland, Vasiliy O. Sysoev, Dylan T. Murray, Robert Tycko, Steven L. McKnight
Summary: Low complexity head domains of neurofilament light and desmin intermediate filaments can switch between conformational disorder and beta-strand-enriched polymers. Solid-state NMR studies reveal spectral patterns consistent with structural order, and phosphorylation and disease-causing mutations can alter self-association of these domains. The facultative structural assembly via labile, beta-strand-enriched self-interactions may broadly influence cell morphology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jingyi Huang, Hongliang Li, Yao Zhang, Jiaye Liu, Hongying Cao, Yongling Long
Summary: Overactive bladder (OAB) is a common chronic symptom complex with a high prevalence in women worldwide and has caused a social burden. The pathogenesis of OAB remains unclear. In this study, a rat model of OAB was established and it was found that the rats in the model group had significantly increased urine volume, urodynamic parameters, contractile responses of detrusor muscles, bladder wall thickness, muscle-to-collagen ratio, as well as the gene and protein expression levels of choline acetyltransferase (ChAT) and solute carrier family 17 member 9 (SLC17A9). These findings suggest that OAB may be associated with increased excitatory purinergic and cholinergic expression.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Saima Usman, Hebah Aldehlawi, Thuan Khanh Ngoc Nguyen, Muy-Teck Teh, Ahmad Waseem
Summary: This study systematically investigated the effects of different sized tags on the formation of vimentin filaments. The results showed that N-terminally tagged vimentin failed to form filaments and destabilized when integrated into a pre-existing network.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Elvira Infante, Sandrine Etienne-Manneville
Summary: Cell migration is a dynamic process crucial for the development of multicellular organisms and immune system responses. The cytoskeletal component known as intermediate filaments plays a key role in cell migration by integrating mechanical properties and signaling functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Jagmohan Singh, Ipsita Mohanty, Satish Rattan
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2018)
Article
Pharmacology & Pharmacy
Ipsita Mohanty, Subas Chandra Parija, Sujit Suklabaidya, Satish Rattan
EUROPEAN JOURNAL OF PHARMACOLOGY
(2018)
Article
Pharmacology & Pharmacy
Matthew M. Meleka, Alethia J. Edwards, Jingsheng Xia, Shelby A. Dahlen, Ipsita Mohanty, Matthew Medcalf, Shaili Aggarwal, Kevin D. Moeller, Ole V. Mortensen, Patrick Osei-Owusu
PHARMACOLOGICAL RESEARCH
(2019)
Article
Pathology
Chellappagounder Thangavel, Cristiano M. Gomes, Stephen A. Zderic, Elham Javed, Sankar Addya, Jagmohan Singh, Sreya Das, Ruth Birbe, Robert B. Den, Satish Rattan, Deepak A. Deshpande, Raymond B. Penn, Samuel Chacko, Ettickan Boopathi
AMERICAN JOURNAL OF PATHOLOGY
(2019)
Article
Multidisciplinary Sciences
Ipsita Mohanty, Jagmohan Singh, Satish Rattan
SCIENTIFIC REPORTS
(2019)
Article
Gastroenterology & Hepatology
Arjun Singh, Ipsita Mohanty, Jagmohan Singh, Satish Rattan
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Pawan Sharma, Santosh K. Yadav, Sushrut D. Shah, Elham Javed, John M. Lim, Shi Pan, Ajay P. Nayak, Reynold A. Panettieri, Raymond B. Penn, Taku Kambayashi, Deepak A. Deshpande
Summary: This study demonstrated that inhibition of DGK disrupted the DAG:PA ratio, resulting in the inhibition of Gq-PLC activation in a negative feedback manner, thus protecting against ASM contraction.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Benjamin Lau, Hossein Fazelinia, Ipsita Mohanty, Serena Raimo, Margarita Tenopoulou, Paschalis-Thomas Doulias, Harry Ischiropoulos
Summary: This study identified and analyzed S-nitrosocysteine residues in mouse heart proteins, with a significant enrichment of these modifications in mitochondrial proteins participating in metabolism. The major functional pathways impacted by protein S-nitrosylation include regulatory proteins in glycolysis, TCA cycle, oxidative phosphorylation, and ATP production. The meta-analysis of the proteomic data supports the hypothesis that nitric oxide signaling via protein S-nitrosylation is a regulator of cardiomyocyte metabolism to ensure optimal cardiac function.
Article
Genetics & Heredity
Ipsita Mohanty, Sudeshna Banerjee, Arabinda Mahanty, Sasmita Mohanty, Nihar Ranjan Nayak, Subas Chandra Parija, Bimal Prasanna Mohanty
Summary: This study investigated acid stress-induced vasorelaxation using a goat mesenteric artery model and identified proteins associated with vasorelaxation. The results showed that the expression of actin and ATP synthase beta increased under acidosis, which may contribute to the altered vasoreactivity observed.
Meeting Abstract
Hematology
Ipsita Mohanty, Jennifer Koch, Patrick Osei-Owusu
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Meeting Abstract
Peripheral Vascular Disease
Patrick Osei Owusu, Ipsita Mohanty, Jennifer N. Koch, James A. McCormick
Article
Chemistry, Medicinal
Subash Chandra Parija, Ipsita Mohanty
PHARMACOGNOSY MAGAZINE
(2018)
Meeting Abstract
Peripheral Vascular Disease
Shelby A. Dahlen, Ipsita Mohanty, Ursula Djameh, Alethia Edwards, Jennifer Koch, Patrick Osei Owusu