Article
Biochemistry & Molecular Biology
Semiha Dede, Mehmet Taspinar, Veysel Yuksek, Sedat Cetin, Ayse Usta
Summary: This study evaluated the protective effect of vitamin D against cytotoxicity caused by fluoride exposure. The findings demonstrated that vitamin D administration reduced the impact of fluoride on bone cells, leading to decreased expression of genes involved in apoptotic, autophagic, and necrotic pathways. This provides important insights into the molecular basis of vitamin D's protective and therapeutic effect against fluoride toxicity.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yu Jin Kim, Se-Kyeong Jang, Sung-Eun Hong, Chan Sub Park, Min-Ki Seong, Hyun-Ah Kim, Ki Soo Park, Chun-Ho Kim, In-Chul Park, Hyeon-Ok Jin
Summary: Targeting the YAP/TAZ-PSAT1 axis can sensitize tamoxifen-resistant MCF7 breast cancer cells by modulating the mTORC1-survivin axis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
G. R. Hancock, K. S. Young, D. J. Hosfield, C. Joiner, E. A. Sullivan, Y. Yildiz, M. Laine, G. L. Greene, S. W. Fanning
Summary: This study identified chemically unconventional antagonists of estrogen receptor alpha (ERα) that have moderate effects on the transcriptional activities of breast cancer cells, exhibit anti-proliferative properties, and engage transcriptional pathways similar to selective estrogen receptor degraders.
Article
Biochemistry & Molecular Biology
Lukasz Bozycki, Joanna Mroczek, Laurence Bessueille, Saida Mebarek, Rene Buchet, Slawomir Pikula, Agnieszka Strzelecka-Kiliszek
Summary: Saos-2 cells, an osteosarcoma cell line, demonstrated a better ability to mineralize compared to hFOB 1.19 cells, an osteoblastic cell line. Apatites were identified in MVs derived from Saos-2 cells, while hFOB 1.19 cells only contained amorphous calcium phosphate complexes. The presence of AnxA2, AnxA6, and FetuA were more pronounced in the plasma membrane of Saos-2 cells compared to hFOB 1.19 cells, suggesting a difference in mineralization process between the two cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hermizi Hapidin, Fairuza Munirah Mazlan, Hasmah Abdullah, Ima Nirwana Soelaiman
Summary: The semi-purified fractions of Quercus infectoria gall extract were found to enhance proliferation, improve mineralisation, and increase ALP activity of human foetal osteoblast cell line (hFOB 1.19).
Article
Cell Biology
Charly Jehanno, Frederic Percevault, Noureddine Boujrad, Pascale Le Goff, Coralie Fontaine, Jean-Francois Arnal, Michael Primig, Farzad Pakdel, Denis Michel, Raphael Metivier, Gilles Flouriot
Summary: Activation and nuclear accumulation of MRTFA in ER alpha positive breast cancer cells remodels both ER alpha location and functions by shifting its activity from nuclear genome regulation to extra-nuclear non-genomic signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Oncology
Taronish D. Dubash, Aditya Bardia, Brian Chirn, Brittany A. Reeves, Joseph A. LiCausi, Risa Burr, Ben S. Wittner, Sumit Rai, Hitisha Patel, Teeru Bihani, Heike Arlt, Francois-Clement Bidard, Virginia G. Kaklamani, Philippe Aftimos, Javier Cortes, Simona Scartoni, Alessio Fiascarelli, Monica Binaschi, Nassir Habboubi, A. John Iafrate, Mehmet Toner, Daniel A. Haber, Shyamala Maheswaran
Summary: This study compared the clinical outcomes of HR+ breast cancer patients who had received fulvestrant treatment with elacestrant and endocrine therapy. The results indicated that elacestrant had better efficacy than fulvestrant in patients with advanced cancers and diverse treatment histories. Patient-derived models were used to validate the sensitivity of elacestrant.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Chemistry, Medicinal
Jiawang Liu, Nirmal Rajasekaran, Ahamed Hossain, Changde Zhang, Shanchun Guo, Borui Kang, Hunsoon Jung, Hongjoong Kim, Guangdi Wang
Summary: Fulvestrant-3-boronic acid (ZB716), an oral selective estrogen receptor degrader (SERD) under clinical development, has been investigated in ADME studies to characterize its absorption, metabolism, and pharmacokinetics. ZB716 exhibits high plasma protein binding and low intestinal mucosal permeability, with high clearance in hepatocytes and metabolism primarily by CYP2D6 and CYP3A. In enzyme activity assays, ZB716 induces certain cytochrome P450 enzymes in a concentration-dependent manner. Pharmacokinetic studies in rats and dogs demonstrate oral bioavailability and dose-proportional drug exposure, with drug accumulation as measured by maximum concentration and area under the concentration-time curve (AUC).
Article
Oncology
Qian Liu, Yutao Shen, Yujia Xiong, Jiwei Bai, Yazhuo Zhang, Chuzhong Li
Summary: A study on gonadotropin-type pituitary adenoma tissue specimens and transcriptome-sequencing data revealed that low-level ESR1 expression was associated with longer progression-free survival in patients. The ErbB signaling pathway and STAT5B gene were found to be related to ESR1. The ER inhibitors AZD9496 and fulvestrant showed significant inhibitory effects on pituitary adenoma growth in vitro and in vivo, potentially through blocking the JAK2/STAT5B pathway.
Article
Endocrinology & Metabolism
Aylin Del Moral-Morales, Juan Carlos Gonzalez-Orozco, Ana Maria Hernandez-Vega, Karina Hernandez-Ortega, Karla Mariana Pena-Gutierrez, Ignacio Camacho-Arroyo
Summary: A study found that in human Glioblastomas (GBM), the pro-oncogenic actions of 17 beta-estradiol (E2) are mediated by EZH2, which promotes proliferation, migration, and invasion of GBM cells. Although E2 does not modify EZH2 expression, gene silencing and pharmacological inhibition of EZH2 can block these pro-oncogenic actions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Engin Kut, Serkan Menekse
Summary: This study retrospectively analyzed mHRPBC patients receiving fulvestrant in our center to evaluate its efficacy and clinical outcomes, and identify factors affecting its efficacy and clinical outcomes. The results showed that fulvestrant is effective in mHRPBC, particularly in patients with BMI under 30, without brain metastasis, without prior chemotherapy, under 65 years of age, and using fulvestrant in the early treatment line. The efficacy of fulvestrant may vary according to age and BMI.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Oncology
Kenichi Watanabe, Naoki Niikura, Yuichiro Kikawa, Mari Oba, Kokoro Kobayashi, Hiroshi Tada, Shinji Ozaki, Uhi Toh, Yutaka Yamamoto, Michiko Tsuneizumi, Toshitaka Okuno, Nobutaka Iwakuma, Takashi Takeshita, Takayuki Iwamoto, Hiroshi Ishiguro, Norikazu Masuda, Shigehira Saji
Summary: The combination of fulvestrant and palbociclib is a potentially safe and effective treatment option for patients with hormone receptor-positive/human epidermal growth factor receptor 2-negative metastatic breast cancer.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Multidisciplinary Sciences
Isti Daruwati, Abednego Kristande Gwiharto, Ahmad Kurniawan, Isa Mahendra, Tri Hanggono Achmad, Mukh Syaifudin, Muchtaridi Muchtaridi
Summary: The study examined the stability of estradiol under different storage conditions using radiolabeled compounds, and found that the internalization rate of estradiol in T-47D cells was significantly higher than in MCF7 cells.
Article
Peripheral Vascular Disease
Lauren A. Biwer, Brigett V. Carvajal, Qing Lu, Joshua J. Man, Iris Z. Jaffe
Summary: The study showed that deletion of endothelial MR in obese female animals can improve vascular dilation, and endothelial ER alpha plays a positive role in increasing nitric oxide to rescue vasodilation. MR and ER alpha may compete for striatin binding within endothelial cells to regulate NO.
Article
Biochemistry & Molecular Biology
Agnieszka Anna Rawluszko-Wieczorek, Julia Lipowicz, Marta Nowacka, Kamila Ostrowska, Paulina Pietras, Malgorzata Blatkiewicz, Marcin Rucinski, Pawel Piotr Jagodzinski, Michal Nowicki
Summary: The presence of estrogen receptor beta (ER beta) is inversely correlated with the occurrence of colorectal cancer (CRC), and low expression of ER beta is associated with poorer overall survival of CRC patients. ER beta can inhibit tumorigenesis through various molecular pathways and also has an impact on the hypoxic response in CRC cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Critical Care Medicine
Daniel Kotok, Libing Yang, John W. Evankovich, William Bain, Daniel G. Dunlap, Faraaz Shah, Yingze Zhang, Dimitris Manatakis, Panayiotis Benos, Ian J. Barbash, Sarah F. Rapport, Janet S. Lee, Alison Morris, Bryan J. McVerry, Georgios D. Kitsios
JOURNAL OF CRITICAL CARE
(2020)
Article
Biochemistry & Molecular Biology
Xiaoyu Ge, Vineet K. Raghu, Panos K. Chrysanthis, Panayiotis Benos
NUCLEIC ACIDS RESEARCH
(2020)
Article
Critical Care Medicine
Georgios D. Kitsios, Haopu Yang, Libing Yang, Shulin Qin, Adam Fitch, Xiao-Hong Wang, Katherine Fair, John Evankovich, William Bain, Faraaz Shah, Kelvin Li, Barbara Methe, Panayiotis Benos, Alison Morris, Bryan J. McVerry
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Article
Respiratory System
Eleanor Valenzi, Haopu Yang, John C. Sembrat, Libing Yang, Spencer Winters, Rachel Nettles, Daniel J. Kass, Shulin Qin, Xiaohong Wang, Michael M. Myerburg, Barbara Methe, Adam Fitch, Jonathan K. Alder, Panayiotis Benos, Bryan J. McVerry, Mauricio Rojas, Alison Morris, Georgios D. Kitsios
Summary: The study revealed the heterogeneity of lung microbiota in patients with IPF, with different bacterial loads and compositions associated with distinct clinical outcomes.
Article
Multidisciplinary Sciences
Nayra Cardenes, John Sembrat, Kentaro Noda, Tyler Lovelace, Diana Alvarez, Humberto E. Trejo Bittar, Brian J. Philips, Mehdi Nouraie, Panayiotis V. Benos, Pablo G. Sanchez, Mauricio Rojas
Summary: The study suggests that EVLP of diseased human lungs is suitable for translational research on chronic lung diseases. By perfusing the lungs for 6 hours with EVLP, researchers were able to assess airway mechanics, pulmonary hemodynamics, gas exchange, and biochemical parameters continuously. Tissue biopsies were also collected to isolate RNA and DNA for identifying unique gene expressions of different diseases, demonstrating the utility of EVLP as a relevant experimental model for understanding pulmonary pathophysiology and various human lung diseases.
SCIENTIFIC REPORTS
(2021)
Letter
Critical Care Medicine
Tamara Cruz, Minxue Jia, John Sembrat, Tracy Tabib, Naomi Agostino, Tullia C. Bruno, Dario Vignali, Pablo Sanchez, Robert Lafyatis, Ana L. Mora, Panayiotis Benos, Mauricio Rojas
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Respiratory System
Milica Vukmirovic, Xitin Yan, Kevin F. Gibson, Mridu Gulati, Jonas C. Schupp, Giuseppe DeIuliis, Taylor S. Adams, Buqu Hu, Antun Mihaljinec, Tony N. Woolard, Heather Lynn, Nkiruka Emeagwali, Erica L. Herzog, Edward S. Chen, Alison Morris, Joseph K. Leader, Yingze Zhang, Joe G. N. Garcia, Lisa A. Maier, Ronald G. Collman, Wonder P. Drake, Michael J. Becich, Harry Hochheiser, Steven R. Wisniewski, Panayiotis V. Benos, David R. Moller, Antje Prasse, Laura L. Koth, Naftali Kaminski
Summary: The study identified specific gene expression patterns in bronchoalveolar lavage (BAL) cells that characterize major pulmonary phenotypes of sarcoidosis, suggesting the presence of distinct disease molecular endotypes. Major associations were found between transcriptional programs and pulmonary manifestations of sarcoidosis, as well as potential endotypes reflecting different patterns of organ involvement. Validation of these endotypes was performed using gene expression data, pulmonary function tests, and cell differentials from an independent sarcoidosis cohort.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Article
Critical Care Medicine
Vineet K. Raghu, Christopher M. Horvat, Patrick M. Kochanek, Ericka L. Fink, Robert S. B. Clark, Panayiotis V. Benos, Alicia K. Au
Summary: This study aimed to evaluate the relationships between serum biomarkers and clinical variables with the development of neurologic complications, finding that neuronal and astrocyte biomarkers may be direct causal determinants for the development of these complications.
PEDIATRIC CRITICAL CARE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Tracy Tabib, Mengqi Huang, Nina Morse, Anna Papazoglou, Rithika Behera, Minxue Jia, Melissa Bulik, Daisy E. Monier, Panayiotis V. Benos, Wei Chen, Robyn Domsic, Robert Lafyatis
Summary: Research indicates that dermal myofibroblasts in systemic sclerosis arise in a two-step process from progenitor fibroblasts expressing SFRP2/DPP4. These cells exhibit distinct transcriptome expression patterns, with some showing signs of proliferation. Only a fraction of progenitor cells differentiate into myofibroblasts under the control of various upstream transcription factors.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Laura M. Molina, Junjie Zhu, Qin Li, Tirthadipa Pradhan-Sundd, Yekaterina Krutsenko, Khaled Sayed, Nathaniel Jenkins, Ravi Vats, Bharat Bhushan, Sungjin Ko, Shikai Hu, Minakshi Poddar, Sucha Singh, Junyan Tao, Prithu Sundd, Aatur Singhi, Simon Watkins, Xiaochao Ma, Panayiotis Benos, Andrew Feranchak, George Michalopoulos, Kari Nejak-Bowen, Alan Watson, Aaron Bell, Satdarshan P. Monga
Summary: YAP1 plays a key role in bile duct development, while hepatocytes achieve adaptation through reduced metabolic and synthetic function to survive for 8 months, blocking the formation of the biliary tree.
Article
Medicine, Research & Experimental
Georgios D. Kitsios, Daniel Kotok, Haopu Yang, Malcolm A. Finkelman, Yonglong Zhang, Noel Britton, Xiaoyun Li, Marina S. Levochkina, Amy K. Wagner, Caitlin Schaefer, John J. Villandre, Rui Guo, John W. Evankovich, William Bain, Faraaz Shah, Yingze Zhang, Barbara A. Methe, Panayiotis Benos, Bryan J. McVerry, Alison Morris
Summary: The study suggests that elevated levels of BDG in critically ill patients are associated with inflammation and poor outcomes. BDG measurements can predict ventilator-free days and survival, and may translocate from either the lung or gut compartment.
Editorial Material
Physiology
George Bebis, Doron Levy, Russell Rockne, Ernesto Augusto Bueno Da Fonseca Lima, Panayiotis V. Benos
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Computer Science, Artificial Intelligence
Xin Bing, Tyler Lovelace, Florentina Bunea, Marten Wegkamp, Sudhir Pai Kasturi, Harinder Singh, Panayiotis Benos, Jishnu Das
Summary: High-dimensional cellular and molecular profiling of biological samples highlights the need for analytical approaches that can integrate multi-omic datasets to generate prioritized causal inferences. Here, we present Essential Regression (ER), a novel interpretable machine-learning approach that addresses these problems by identifying latent factors and their likely cause-effect relationships with system-wide outcomes/properties of interest. ER outperforms other methods in terms of prediction and can be coupled with probabilistic graphical modeling to strengthen causal inferences. The utility of ER is demonstrated using multi-omic system immunology datasets to generate and validate novel cellular and molecular inferences in various contexts.
Article
Cell Biology
Anthony R. Cillo, Ashwin Somasundaram, Feng Shan, Carly Cardello, Creg J. Workman, Georgios D. Kitsios, Ayana T. Ruffin, Sheryl Kunning, Caleb Lampenfeld, Sayali Onkar, Stephanie Grebinoski, Gaurav Deshmukh, Barbara Methe, Chang Liu, Sham Nambulli, Lawrence P. Andrews, W. Paul Duprex, Alok Joglekar, Panayiotis Benos, Prabir Ray, Anuradha Ray, Bryan J. McVerry, Yingze Zhang, Janet S. Lee, Jishnu Das, Harinder Singh, Alison Morris, Tullia C. Bruno, Dario A. A. Vignali
Summary: Through extensive analysis, it has been found that immune cell states are closely related to mortality in critically ill patients with COVID-19, and single-cell RNA sequencing is helpful in deciphering immune states. Additionally, persistently high levels of interferon signaling module and upregulation of CXCL10 in SARS-CoV-2-infected myeloid cells are associated with an increased risk of death.
CELL REPORTS MEDICINE
(2021)
Article
Biochemical Research Methods
Vineet K. Raghu, Xiaoyu Ge, Arun Balajiee, Daniel J. Shirer, Isha Das, Panayiotis Benos, Panos K. Chrysanthis
Summary: Genome sequencing technologies have the potential to transform clinical decision making and biomedical research by enabling high-throughput measurements of the genome at a granular level. It is important to integrate high-throughput genomic data with demographic, phenotypic, environmental, and behavioral information, and infer relationships between these data types for better understanding of disease mechanisms and prediction of medical interventions. A new methodology called piPref-Div has been proposed to select informative variables for probabilistic graphical models, improving breast cancer outcome prediction and providing biologically interpretable views of gene expression data.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2021)