Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 50, Issue 4, Pages 505-514Publisher
WILEY
DOI: 10.1002/eji.201948355
Keywords
Avidity regulation; Functional avidity; Prime; boost; T-cell receptor affinity; T-cell vaccination
Categories
Funding
- NIBIB NIH HHS [R01 EB022433] Funding Source: Medline
- The Swiss National Science Foundation [310030-179459] Funding Source: Medline
- Cancer Research Institute Funding Source: Medline
- Ludwig Institute for Cancer Research Funding Source: Medline
- Alfred and Annemarie von Sick Funding Source: Medline
- Université de Lausanne Funding Source: Medline
- Swiss Cancer Research [3971-08-2016, 4291-08-2017] Funding Source: Medline
Ask authors/readers for more resources
It is known that for achieving high affinity antibody responses, vaccines must be optimized for antigen dose/density, and the prime/boost interval should be at least 4 weeks. Similar knowledge is lacking for generating high avidity T-cell responses. The functional avidity (FA) of T cells, describing responsiveness to peptide, is associated with the quality of effector function and the protective capacity in vivo. Despite its importance, the FA is rarely determined in T-cell vaccination studies. We addressed the question whether different time intervals for short-term homologous vaccinations impact the FA of CD8 T-cell responses. Four-week instead of 2-week intervals between priming and boosting with potent subunit vaccines in C57BL/6 mice did not improve FA. Equally, similar FA was observed after vaccination with virus-like particles displaying low versus high antigen densities. Interestingly, FA was stable in vivo but not in vitro, depending on the antigen dose and the time interval since T-cell activation, as observed in murine monoclonal T cells. Our findings suggest dynamic in vivo modulation for equal FA. We conclude that low antigen density vaccines or a minimal 4-week prime/boost interval are not crucial for the T-cell's FA, in contrast to antibody responses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available