4.7 Article

Bacterial shifts during in-situ mineralization bio-treatment to nonferrous metal(loid) tailings

Journal

ENVIRONMENTAL POLLUTION
Volume 255, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2019.113165

Keywords

Microbial treatment; Metal(loid) contamination; Bacterial communities; Sulfur metabolism

Funding

  1. National Science Foundation of China [41430106, 41573080, 41720104007, 41711530030, 41711530150]
  2. Ministry of Science and Technology of the Peoples Republic of China [S2016G2135]
  3. Centre National de la Recherche Scientifique, France [CNRS PRC1416]
  4. Royal Society Newton Mobility Grant [IE161198]
  5. National Natural Science Foundation International Joint collaboration China-Sweden [41430106]

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Nonferrous mine tailings have caused serious problems of co-contamination with metal(loid)s. It is still a global challenge to cost-effectively manage and mitigate the effect of the mining wastes. We conducted an in-situ bio-treatment of non-ferrous metal(loid) tailings using a microbial consortium of sulfate reducing bacteria (SRB). During the bio-treatment, the transformation of metal(loid)s (such as Cu, Fe, Mn, Pb, Sb, and Zn) into oxidizable and residual fractions in the subsurface tended to be higher than that observed in the surface. As well the mineral compositions changed becoming more complex, indicating that the sulfur reducing process of bio-treatment shaped the bio-transformation of metal(loid)s. The added SRB genera, especially Desulfotomaculum genus, colonized the tailings suggesting the coalescence of SRB consortia with indigenous communities of tailings. Such observation provides new insights for understanding the functional microbial community coalescence applied to bio-treatment. PICRUSt analysis revealed presence of genes involved in sulfate reduction, both assimilatory and dissimilatory. The potential for the utilization of both inorganic and organic sulfur compounds as S source, as well as the presence of sulfite oxidation genes indicated that SRB play an important role in the transformation of metal(loid)s. We advocate that the management of microorganisms involved in S-cycle is of paramount importance for the in situ bio-treatment of tailings, which provide new insights for the implementation of bio-treatments for mitigating the effect of tailings. (C) 2019 Published by Elsevier Ltd.

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