Article
Biochemistry & Molecular Biology
Guangqing Du, Youduo Wu, Wei Kang, Yongping Xu, Shuying Li, Chuang Xue
Summary: In this study, the strain Clostridium acetobutylicum ATCC 55025 and its mutant HKKO were engineered to improve butanol production. Knocking out the acetoacetate decarboxylase gene adc decreased acetone production and increased the butanol/ABE ratio. Overexpression of the acetaldehyde/alcohol dehydrogenase gene adhE2 further increased butanol production.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Guangqing Du, Chao Zhu, Mengmeng Xu, Lan Wang, Shang-Tian Yang, Chuang Xue
Summary: This study identified four orphan histidine kinases (HKs) that control sporulation and solventogenesis in Clostridium acetobutylicum. Single knockout of cac3319 halted sporulation at the initiation stage but increased butanol tolerance and production, while cac0437 knockout caused premature sporulation and inhibited the transition to solventogenesis, resulting in low butanol production.
Article
Energy & Fuels
Zhiyou Zhou, Yujie Jing, Saijin Wei, Qinghua Zhang, Shuaiying Peng, Xuejiao An, Hanguang Li
Summary: In this study, an exogenous regulatory strategy named oxidation-reduction potential (ORP) regulation was used to enhance butanol production. The findings showed that controlling the ORP value at -400 mV resulted in the highest butanol production, with increased yield and productivity. The study also investigated the physiological mechanisms of ORP regulation on butanol synthesis.
Article
Biotechnology & Applied Microbiology
Guangqing Du, Jian Che, Youduo Wu, Zhenzhong Wang, Zhiyang Jiang, Feng Ji, Chuang Xue
Summary: In this study, the disruption of hydA gene in Clostridium acetobutylicum led to increased butanol production and decreased acetone formation. Exogenous supplementation of methyl viologen further improved the butanol yield and ratio. The engineered strain showed potential for producing biobutanol using lignocellulosic biomass.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Energy & Fuels
F. Raganati, A. Procentese, G. Olivieri, M. E. Russo, P. Salatino, A. Marzocchella
Summary: This study demonstrates the intensive production of butanol using immobilized cells of Clostridium acetobutylicum in a novel continuous fermentation system. By implementing a stagewise simulated moving bed design and integrating the reaction and the product recovery sections, the system achieves high productivity and concentration of butanol.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Agricultural Engineering
Ruiling Gao, Lian Xiong, Mengkun Wang, Fen Peng, Hairong Zhang, Xinde Chen
Summary: Coupled fermentation of Clostridium acetobutylicum and oleaginous yeasts was conducted to produce acetone-butanol-ethanol (ABE) and lipids from sugarcane molasses. The results showed that the ABE fermentation wastewaters could be effectively treated and the added value of the products could be increased through coupling with lipid-producing fermentation process. Different types of wastewaters were analyzed and classified based on their carbon source content and structure. Oleaginous yeasts, including Trichosporon dermatis and Lipomyces starkeyi, were cultured on the two types of wastewaters and their lipid productions and removals of COD and ammonia were evaluated. The findings suggest that Trichosporon dermatis performed well on both types of wastewaters, while Lipomyces starkeyi achieved the highest lipid production on Type-2 wastewater.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Ibham Veza, Mohd Farid Muhamad Said, Zulkarnain Abdul Latiff
Summary: This review article discusses the history of the butanol industry and recent advances in butanol production by ABE fermentation, highlighting novel approaches such as searching for abundant and inexpensive substrates and optimizing processes. The challenges of biobutanol production, including low yield and end-product toxicity, as well as potential research routes, are summarized, along with the future research direction of butanol production by ABE fermentation.
BIOMASS & BIOENERGY
(2021)
Article
Biochemistry & Molecular Biology
Youduo Wu, Zhenzhong Wang, Xiao Ma, Chuang Xue
Summary: By optimizing high-temperature simultaneous saccharification and fermentation (SSF) technology and using genetically engineered thermotolerant Clostridium acetobutylicum, the production and productivity of lignocellulosic butanol were successfully increased, demonstrating its potential in improving butanol production efficiency.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Karlo Guerrero, Roberto Gallardo, Ernesto Gonzalez, Fabian Veliz, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: By adding butyrate and neutral red to the culture medium, and using electro-fermentation with an external electron source, the production of butanol can be increased. However, the presence of butyrate and the absence of neutral red in electro-fermentation are detrimental to the microorganism.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Jiapen Wang, Susu Zhang, Chunhai Li, Xinli Liu, Zhenshang Xu, Ting Wang
Summary: In this study, the N-terminal 20 amino acid of Lactobacillus amylovorus feruloyl esterase (N20) was used as a signal peptide to fuse with a xylanase Xyl derived from Bacillus paralicheniformis. The fused protein N20-Xyl could be effectively secreted from E. coli BL21(DE3) cells. Enzymatic characterization showed that Xyl and N20-Xyl had similar properties with optimal pH of 8.0 and temperature of 50°C. Xylooligosaccharides were prepared through simultaneous xylanase secretion and xylan degradation by using E. coli, with the main products being xylobiose, xylotriose, and xylotetraose. Moreover, several Lactobacillus strains were found to efficiently utilize these xylooligosaccharides for growth.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Daniele Molognoni, Maria Vega, Truus de Vrije, Ana Lopez Contreras, Montse Bosch, Pau Bosch-Jimenez, Eduard Borras
Summary: This study investigated glucose-based ABE electro-fermentation and found that setting the cathode potential (E-cat) at -0.8 V resulted in the optimal EF scenario for C. beijerinckii, leading to a higher specific growth rate and improved butanol production. The addition of 0.5 mM neutral red as a redox mediator further enhanced butanol productivity, with a very low energy consumption of 0.02 kWh/kg-butanol.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wei-Lun Chang, Wenjie Hou, Mengmeng Xu, Shang-Tian Yang
Summary: This study achieved continuous production of butanol from glucose and butyrate by immobilizing cells in a single-pass fibrous-bed bioreactor. The high density of immobilized cells improved the tolerance to butanol and butyric acid, resulting in significantly increased productivity.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Victor E. Balderas Hernandez, Carlos J. Salas-Montantes, Ana P. Barba-De la Rosa, Antonio De Leon-Rodriguez
Summary: This study aimed to achieve the autodisplay of the endo beta-1,4-xylanase (XynA) from Clostridium cellulovorans in Escherichia coli using the AIDA system for whole-cell biocatalysis. Optimal operational conditions were found to be at a temperature of 55 degrees C and pH 6.5, with xylanase activity inhibited by certain ions and compounds but improved by others, such as Ca2+, Co2+, and Mn2+. The results indicate that the pAIDA-xynA vector has the capability to express functional xylanase for whole-cell biocatalysis in hydrolysing xylans from hemicellulose feedstock.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Engineering, Environmental
Marwa M. El-Dalatony, Bikram Basak, Mayur B. Kurade, Hyun-Seog Roh, Min Jang, Byong-Hun Jeon
Summary: This study demonstrates the effective production of biohydrogen (bioH(2)) and ABE through ABE fermentation using sonication pretreated Chlamydomonas mexicana biomass as a cost-effective substrate. Sonication enhances the recovery and bioaccessibility of carbohydrates and proteins, resulting in higher yields of bioH(2) and volatile fatty acids. The study shows the efficient conversion of soluble carbohydrates and proteins into bioH(2) and ABE, which can be used for high-energy biofuel production with minimal waste generation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Energy & Fuels
Hiram Ramirez-Lagunes, Maria Guadalupe Aguilar-Uscanga, Maria Ines Infanzon-Rodriguez, Bernardo Sachman-Ruiz, Javier Gomez-Rodriguez, Cirilo Nolasco-Hipolito, Sandra del Moral
Summary: This study optimized xylanase production by evaluating different carbon and nitrogen sources, finding sugarcane bagasse to be the best carbon source and ammonium sulfate to be the best nitrogen source. These optimal conditions increased xylanase activity significantly, showing feasibility of producing xylanases using low-cost substrates and optimizing operating conditions.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Agriculture, Dairy & Animal Science
Abubakar Bello, Jerome Henri, Alexis Viel, Jonathan Paul Mochel, Blazej Pozniak
Summary: Poultry production is associated with the use of veterinary medicinal products to manage diseases. Ionophore coccidiostats, although prohibited for use in laying hens, are frequently found in commercial poultry eggs. Various factors influence the presence of residues in eggs, with the carryover of coccidiostats from feed to laying hens being the main reason. This paper reviews the causes of residue occurrence and highlights the potential application of pharmacokinetic modeling for predicting residue transfer in eggs.
Article
Geriatrics & Gerontology
Parvin Mohammadiarvejeh, Brandon S. Klinedinst, Qian Wang, Tianqi Li, Brittany Larsen, Amy Pollpeter, Shannin N. Moody, Sara A. Willette, Jon P. Mochel, Karin Allenspach, Guiping Hu, Auriel A. Willette
Summary: This study utilized longitudinal data from the UK Biobank to investigate cognitive aging in mid-life. The findings revealed the existence of a group of individuals who exhibited positive-aging, with cognitive performance similar to younger adults. Furthermore, the study identified vascular health and cellular bioenergetics as important biological factors associated with optimal cognitive aging, irrespective of age, sex, or socioeconomic status. These results suggest that lifestyle and pharmacological interventions may contribute to optimizing cognitive aging.
Article
Veterinary Sciences
Alec H. Sherman, Karl H. Kraus, Danielle Watt, Lingnan Yuan, Jonathan P. Mochel
Summary: This retrospective study evaluated the use of linear external skeletal fixation (ESF) in the treatment of nonarticular tibial fractures in dogs and cats. The study found that linear ESF applied using minimally invasive techniques was effective in the treatment of these fractures and could be considered as a minimally invasive option.
VETERINARY SURGERY
(2023)
Article
Veterinary Sciences
Jessica Perrucci, Rebecca Walton, Chelsea Zorn, Lingnan Yuan, Jonathan P. Mochel, April Blong
Summary: This study evaluated the use of inhalant anesthesia vs sedation for urinary catheter placement in male cats with urethral obstruction. The results showed no significant difference in complications or recurrence rates between inhalant anesthesia and sedation. Baseline serum biochemical data were significantly associated with complications, survival, and short-term recurrence rates.
JOURNAL OF FELINE MEDICINE AND SURGERY
(2023)
Article
Multidisciplinary Sciences
Benjamin K. Schneider, Jessica Ward, Samantha Sotillo, Catherine Garelli-Paar, Emilie Guillot, Marc Prikazsky, Jonathan P. Mochel
Summary: The RAAS plays a central role in the pathophysiology of congestive heart failure (CHF), justifying the use of ACE inhibitors (ACEi) in dogs and humans with cardiac diseases. Previous studies have shown that the pharmacological action of benazepril is relatively independent of doses greater than 0.25 mg/kg P.O., providing a rationale for the European labeled dose of benazepril in dogs with CHF. However, these studies relied on measures of ACE activity, which is a sub-optimal endpoint for characterizing the effect of ACEi on the RAAS.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Dipak Kumar Sahoo, Marilyn N. Martinez, Kimberly Dao, Vojtech Gabriel, Christopher Zdyrski, Albert E. Jergens, Todd Atherly, Chelsea A. Iennarella-Servantez, Laura E. Burns, Dwayne Schrunk, Donna A. Volpe, Karin Allenspach, Jonathan P. Mochel
Summary: To study the effects of drug-intestinal barrier interactions on therapeutic failure or toxicity, new methods are needed to evaluate drug-gut interactions in veterinary medicine. Three-dimensional (3D) organoids can provide a cost-effective system to address these difficulties without invasive in vivo assays. In this study, we demonstrate the growth and differentiation of canine-colonoid-derived intestinal epithelial cells in a 2D monolayer, and evaluate the permeability of passive beta-blockers in this canine-derived 2D model. The results show promising potential for species-specific assessments of drug permeability.
Article
Geriatrics & Gerontology
Brandon S. S. Klinedinst, Mihir K. K. Kharate, Parvin Mohammadiarvejeh, Mohammad Fili, Amy Pollpeter, Brittany A. A. Larsen, Shannin Moody, Qian Wang, Karin Allenspach, Jonathan P. P. Mochel, Auriel A. A. Willette
Summary: Communities around the world face the challenge of an aging population, where age is the primary risk factor for cognitive decline and diseases like Alzheimer's. However, a group of exceptional individuals known as super-agers have been found to maintain a healthy brain and cognitive function in old age. This study identified potential super-agers among UK Biobank participants and found that they have larger brain volume, better cognitive abilities, and less functional connectivity.
Review
Veterinary Sciences
Ronette Gehring, Jonathan P. Mochel, Ivo Schmerold
Summary: In Europe, the WHO, WOAH (formerly OIE), and EMA classification systems are the prevailing standards for guiding the use of antibiotics. These systems provide guidance for choosing antibiotics for both humans and animals. However, there are some differences in the classification of certain substances among these systems. Veterinarians should consider the EMA document for everyday clinical use of antibiotics and consult the OIE list under certain circumstances.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Khashayar Moshksayan, Anirudha Harihara, Sudip Mondal, Evan Hegarty, Todd Atherly, Dipak K. Sahoo, Albert E. Jergens, Jonathan P. Mochel, Karin Allenspach, Janet Zoldan, Adela Ben-Yakar
Summary: The OrganoidChip is a microfluidic imaging platform that efficiently immobilizes intestinal and cardiac organoids without affecting their viability and functionality. It enables high-throughput and high-resolution imaging at speeds much faster than conventional platforms, ensuring clear images and accurate assessment of organoid responses to drug treatment.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
L. Z. Barron, T. C. DeFrancesco, Y. -y. Chou, J. D. Bonagura, M. A. Tropf, S. D. Murphy, A. K. McManamey, L. Yuan, J. P. Mochel, J. L. Ward
Summary: This study aimed to compare sonographic measurements of the caudal vena cava (CVC) in healthy cats and cats with cardiogenic cavitary effusion (CCE), cardiogenic pulmonary edema (CPE), or non-cardiac causes of cavitary effusion (NCE). The results showed that cats with the diseases had larger CVC measurements compared to healthy cats. Furthermore, the caudal vena cava collapsibility index (CVC-CI) was helpful in distinguishing between cardiogenic and non-cardiogenic etiology.
JOURNAL OF VETERINARY CARDIOLOGY
(2023)
Article
Veterinary Sciences
Molly O'Brien, Jonathan P. Mochel, Kevin Kersh, Chong Wang, Jarrod Troy
Summary: This study aimed to investigate the pharmacokinetics and local complications of the NSAID phenylbutazone (PBZ) used for intravenous regional limb perfusion (IVRLP) in horses. The results showed that IVRLP with PBZ at a dose of 2.2 mg/kg did not provide any advantage compared to systemic PBZ administration, and the local complications were similar to placebo-IVRLP.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Nicole Bunnell, April Blong, Debosmita Kundu, Jonathan Paul Mochel, Rebecca Walton
Summary: This retrospective study analyzed the medical records of 33 cats diagnosed with hemoperitoneum between 2018 and 2022. The results showed that nearly half of the cats required blood transfusion, and transfusion was significantly associated with the survival rate.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Karin Allenspach, Chi-Hsuan Sung, Jose Joaquin Ceron, Camila Peres Rubio, Agnes Bourgois-Mochel, Jan S. Suchodolski, Lingnan Yuan, Debosmita Kundu, Joan Colom Comas, Kieran Rea, Jonathan P. Mochel
Summary: This study investigated the effects of DE-CA9 (TM) on healthy Beagle dogs and found that it can reduce oxidative stress markers and have positive effects on protein and lipid metabolism in serum and feces.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Dipak Kumar Sahoo, Karin Allenspach, Jonathan P. Mochel, Valerie Parker, Adam Joseph Rudinsky, Jenessa A. Winston, Agnes Bourgois-Mochel, Mark Ackermann, Romy M. Heilmann, Gabor Koeller, Lingnan Yuan, Tracey Stewart, Shannon Morgan, Kaitlyn R. Scheunemann, Chelsea A. Iennarella-Servantez, Vojtech Gabriel, Christopher Zdyrski, Rachel Pilla, Jan S. Suchodolski, Albert E. Jergens
Summary: Chronic inflammatory enteropathy (CE) is a common cause of persistent gastrointestinal signs and intestinal inflammation in dogs. This study aimed to investigate the effects of the synbiotic-IgY supplement on clinical signs, inflammatory indices, and mucosal microbiota in dogs with CE. Results indicated that dogs administered the supplement showed decreased levels of inflammatory markers, increased numbers of beneficial bacteria, and decreased numbers of harmful bacteria, suggesting a beneficial effect of the supplement on dogs with CE.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Miranda Buseman, April E. Blong, Lingnan Yuan, Jonathan P. Mochel, Rebecca A. L. Walton
Summary: This study evaluated the association between admission total plasma protein (TPP) and red blood cell transfusion in dogs diagnosed with hemoabdomen. The results showed that low TPP was associated with the need for transfusion, regardless of PCV levels. Other factors associated with transfusion administration included PCV levels, bicarbonate, base excess, lactate, and APPLE(fast) scores.
JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE
(2023)
