Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 191, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2020.110231
Keywords
Cadmium; Chlamydomonas reinhardtii; Whole-genome re-sequencing; Transcriptome; Tolerance
Categories
Ask authors/readers for more resources
Cadmium (Cd), a common environmental toxic contaminant, is easily accumulated in living organisms, leading to numerous harmful effects. Chlamydomonas reinhardtii, a unicellular eukaryotic green algae strain, is a very suitable candidate for bioremediation of Cd-contaminated water. However, for the poor resistance to Cd, application of C. reinhardtii was restricted and genes mediating Cd tolerance in C. reinhardtii remain unclear. In this paper, adaptive laboratory evolution was performed with algae constant exposure to Cd over 420-day at environmentally relevant concentrations to select C. reinhardtii strains with high tolerance to Cd. Physiological indicators, such as cell proliferation, photosynthetic pigment contents and photosynthetic activity of photo system were detected to evaluate the Cd tolerance of selected algae strain ALE0.5. Then, whole-genome resequencing and transcriptome were applied to identify the genes related to Cd tolerance. Genes involved in photosynthesis (PSBP1), glutathione metabolism (CHLREDRAFT_167073, GPX5) and calcium transport (CHLR-EDRAFT_189266, CHLREDRAFT_191203, CHLREDRAFT_187187, CSE1) were related to Cd tolerance in C. reinhardtii. This study provides a basis for obtaining transgenic C. reinhardtii strains with high Cd tolerance used for bioremediation of Cd pollution in the future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available